Cytotoxic effect of Berberis libanotica roots extracts on human cancer cells and antioxidant activities

In the present study, we evaluated the cytotoxic effect and the antioxidant activity of methanolic and ethanolic roots extracts obtained from Berberis libanotica a Lebanese medicinal tree. Cytotoxic activity was assessed on the colon cancer HT 29, HCT 116, Caco-2 and breast cancer MCF-7 and MDA-MB-231cell lines, using the MTT viability assay. Both extracts inhibited cancer cells proliferation in a doseand time depending manner without being cytotoxic against the normal MCF-10 cell line. Our results suggest that methanolic extract could induce a caspase-independent cell death in the colon and breast tumor cells HT 29 and MCF-7, respectively. DPPH and FRAP assays showed a moderate to strong antioxidant activity of the methanolic and ethanolic extracts with EC50 values of 0.13 ± 0.001 and 0.1± 0.002 mg/ml, respectively. Collectively, these findings suggest that Berberis libanotica roots could serve as a promising source of antioxidant and anticancer bioactive compounds.

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Cytotoxicity of Meroterpenoids from Sargassum Siliquastrum against Human Cancer Cells

Natural Product Communications ◽

10.1177/1934578x1300800403 ◽

2013 ◽

Vol 8 (4) ◽

pp. 1934578X1300800

Author(s):

Jung Im Lee ◽

Myoung K. Kwak ◽

Hee Y. Park ◽

Youngwan Seo

Keyword(s):

Cancer Cells ◽

Cell Lines ◽

Brown Alga ◽

Cytotoxic Effect ◽

Human Cancer ◽

Human Cancer Cells ◽

Crude Extracts ◽

Bioassay Guided Fractionation ◽

Ht 29 ◽

Mcf 7

The cytotoxicity of the brown alga Sargassum siliquastrum on human cancer cells (AGS, HT-29, HT-1080, and MCF-7) was investigated. Bioassay-guided fractionation of the crude extracts showed that the 85% aq. methanol (MeOH) fraction was the most toxic. Seven known meroterpenoids (1-7) were isolated from this cytotoxic fraction. Each compound was evaluated for its cytotoxic effect on human cancer cells. Compounds 1, 2, and 4 showed strong cytotoxicity against AGS, HT-29, and HT-1080 cell lines, with IC50 values ranging from 0.5 to 5.7 μg/mL.

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Cytotoxic Effect on Human Cancer Cells and Antioxidant Activity of Extracts of Codonopsis lanceolata Root using Different Solvent Fractions

Journal of Crop Science and Biotechnology ◽

10.1007/s12892-018-0154-0 ◽

2018 ◽

Vol 21 (5) ◽

pp. 507-514

Author(s):

Hee Ock Boo ◽

Jeong Hun Park ◽

Hag Hyun Kim ◽

Soo Jeong Kwon ◽

Sun Hee Woo

Keyword(s):

Antioxidant Activity ◽

Cancer Cells ◽

Cytotoxic Effect ◽

Human Cancer ◽

Codonopsis Lanceolata ◽

Human Cancer Cells

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Rhodium(i) N-heterocyclic carbene complexes: synthesis and cytotoxic properties

New Journal of Chemistry ◽

10.1039/d1nj00144b ◽

2021 ◽

Vol 45 (11) ◽

pp. 5176-5183

Author(s):

Ichraf Slimani ◽

Serap Şahin-Bölükbaşı ◽

Mustafa Ulu ◽

Enes Evren ◽

Nevin Gürbüz ◽

...

Keyword(s):

Cancer Cells ◽

Mtt Assay ◽

Incubation Time ◽

Human Cancer ◽

Cytotoxic Activities ◽

Carbene Complexes ◽

Human Cancer Cells ◽

Benzimidazolium Salts ◽

Ht 29

A series of benzimidazolium salts and their [RhCl(NHC)(COD)] complexes were synthesized. All compounds were screened for in vitro cytotoxic activities against a panel of human cancer cells (HT-29 colon, Ishikawa endometrial, U-87 glioblastoma) using the MTT assay for 48 h incubation time.

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Antioxidant and Cytotoxic Activities of Myrtaceae Essential Oils Rich in Terpenoids From Brazil

Natural Product Communications ◽

10.1177/1934578x21996156 ◽

2021 ◽

Vol 16 (2) ◽

pp. 1934578X2199615

Author(s):

Lucas Botelho Jerônimo ◽

Jamile S. da Costa ◽

Laine C. Pinto ◽

Raquel C. Montenegro ◽

William N. Setzer ◽

...

Keyword(s):

Essential Oils ◽

Cancer Cells ◽

Antioxidant Activities ◽

Human Cancer ◽

Psidium Guajava ◽

Cytotoxic Activities ◽

Chemical Compositions ◽

Main Compound ◽

Human Cancer Cells ◽

High Concentrations

This work analyzed the chemical compositions and evaluated the antioxidant and cytotoxic activities of essential oils (EO) of Eugenia patrisii (Epat), Eugenia stipitata (Esti), Myrcia splendens (Mspl), Myrcia sylvatica (Msyl), Psidium guajava (Pgua), and Psidium guineense (Pgui-1 and Pgui-2) from the Brazilian Amazon. Sesquiterpenoids were found in high concentrations in the oils of E. patrisii and M. splendens, which were rich in E-caryophyllene (32.0% and 45.8%); E. stipitata and M. sylvatica, which displayed germacrene D (11.8%) and germacrene B (24.5%); and P. guajava that showed epi-β-bisabolol (16.1%) as the main compound. However, P. guineense samples (Pgui-1 and Pgui-2) were rich in monoterpenoids such as limonene (Pgui-1: 30.2%; Pgui-2 30.4%) and α-pinene (Pgui-1: 22.5%; Pgui-2: 17.7%). The samples showed a weak and moderate antioxidant activities in the DPPH assay, displaying inhibition rates from 11.5% to 38.6% (at 10 mg/mL). All samples were cytotoxic against human cancer cells by the MTT method. Epat oil showed higher activity against melanoma (SKMEL-19, IC505.8 µg/mL), gastric (AGP01, IC503.2 µg/mL), and colon (HCT116, IC506.7 µg/mL). Meanwhile, the samples Pgua and Pgui were more active against breast cancer cells (MCF7, IC5012.4 µg/mL and 11.6 µg/mL, respectively).

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Effect of Calomelanone, a Dihydrochalcone Analogue, on Human Cancer Apoptosis/Regulated Cell Death in an In Vitro Model

BioMed Research International ◽

10.1155/2020/4926821 ◽

2020 ◽

Vol 2020 ◽

pp. 1-16

Author(s):

Wasitta Rachakhom ◽

Ratana Banjerdpongchai

Keyword(s):

Cell Death ◽

Cancer Cells ◽

Hepg2 Cells ◽

Cytotoxic Effect ◽

Human Cancer ◽

Autophagic Flux ◽

Dependent Manner ◽

Anticancer Activities ◽

Human Cancer Cells ◽

Regulated Cell Death

Calomelanone, 2 ′ ,6 ′ -dihydroxy-4,4 ′ -dimethoxydihydrochalcone, possesses anticancer activities. This study was conducted to investigate the cytotoxic effect of calomelanone, a dihydrochalcone analogue, on human cancer cells and its associated mechanisms. The cytotoxic effect of calomelanone was measured by MTT assay. Annexin V-FITC/propidium iodide and DiOC6 staining that employed flow cytometry were used to determine the mode of cell death and reduction of mitochondrial transmembrane potential (MTP), respectively. Caspase activities were measured using specific substrates and colorimetric analysis. The expression levels of Bcl-2 family proteins were determined by immunoblotting. Reactive oxygen species were also measured using 2 ′ ,7 ′ -dihydrodichlorofluorescein diacetate and dihydroethidium (fluorescence dyes). Calomelanone was found to be toxic towards various human cancer cells, including acute promyelocytic HL-60 and monocytic leukemic U937 cells, in a dose-dependent manner at 24 h and human hepatocellular HepG2 cells at 48 h. However, the proliferation of HepG2 cells increased at 24 h. Calomelanone was found to induce apoptosis in HL-60 and U937 at 24 h and HepG2 apoptosis at 48 h via the intrinsic pathway by inducing MTP disruption. This compound also induced caspase-3, caspase-8, and caspase-9 activities. Calomelanone upregulated proapoptotic Bax and Bak and downregulated antiapoptotic Bcl-xL proteins in HepG2 cells. Moreover, signaling was also associated with oxidative stress in HepG2 cells. Calomelanone induced autophagy at 24 h of treatment, which was evidenced by staining with monodansylcadaverine (MDC) to represent autophagic flux. This was associated with a decrease of Akt (survival pathway) and an upregulation of Atg5 (the marker of autophagy). Thus, calomelanone induced apoptosis/regulated cell death in HL-60, U937, and HepG2 cells. However, it also induced autophagy in HepG2 depending on duration, dose, and type of cells. Thus, calomelanone could be used as a potential anticancer agent for cancer treatment. Nevertheless, acute and chronic toxicity should be further investigated in animals before conducting investigations in human patients.

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Inhibition of NADPH Oxidases Activity by Diphenyleneiodonium Chloride as a Mechanism of Senescence Induction in Human Cancer Cells

Antioxidants ◽

10.3390/antiox9121248 ◽

2020 ◽

Vol 9 (12) ◽

pp. 1248

Author(s):

Katarzyna Piszczatowska ◽

Dorota Przybylska ◽

Ewa Sikora ◽

Grażyna Mosieniak

Keyword(s):

Cancer Cells ◽

Therapeutic Potential ◽

Human Cancer ◽

Cell Metabolism ◽

Human Colon ◽

Nadph Oxidases ◽

Human Colon Cancer ◽

Inhibition Of Proliferation ◽

Human Cancer Cells ◽

Mcf 7

NADPH oxidases (NOX) are commonly expressed ROS-producing enzymes that participate in the regulation of many signaling pathways, which influence cell metabolism, survival, and proliferation. Due to their high expression in several different types of cancer it was postulated that NOX promote tumor progression, growth, and survival. Thus, the inhibition of NOX activity was considered to have therapeutic potential. One of the possible outcomes of anticancer therapy, which has recently gained much interest, is cancer cell senescence. The induction of senescence leads to prolonged inhibition of proliferation and contributes to tumor growth restriction. The aim of our studies was to investigate the influence of low, non-toxic doses of diphenyleneiodonium chloride (DPI), a potent inhibitor of flavoenzymes including NADPH oxidases, on p53-proficient and p53-deficient HCT116 human colon cancer cells and MCF-7 breast cancer cells. We demonstrated that the temporal treatment of HCT116 and MCF-7 cancer cells (both p53 wild-type) with DPI caused induction of senescence, that was correlated with decreased level of ROS and upregulation of p53/p21 proteins. On the contrary, in the case of p53−/− HCT116 cells, apoptosis was shown to be the prevailing effect of DPI treatment. Thus, our studies provided a proof that inhibiting ROS production, and by this means influencing ROS sensitive pathways, remains an alternative strategy to facilitate so called therapy-induced senescence in cancers.

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Regulation of mitochondrial hexokinase in cultured HT 29 human cancer cells. An ultrastructural and biochemical study

Biochimica et Biophysica Acta (BBA) - Biomembranes ◽

10.1016/0005-2736(87)90202-1 ◽

1987 ◽

Vol 902 (3) ◽

pp. 335-348 ◽

Cited By ~ 34

Author(s):

Colette Denis-Pouxviel ◽

Ingrid Riesinger ◽

Christine Bühler ◽

Dieter Brdicza ◽

Jean-Claude Murat

Keyword(s):

Cancer Cells ◽

Human Cancer ◽

Biochemical Study ◽

Human Cancer Cells ◽

Ht 29

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Maytenus disticha Extract and an Isolated β-Dihydroagarofuran Induce Mitochondrial Depolarization and Apoptosis in Human Cancer Cells by Increasing Mitochondrial Reactive Oxygen Species

Biomolecules ◽

10.3390/biom10030377 ◽

2020 ◽

Vol 10 (3) ◽

pp. 377

Author(s):

Iván González-Chavarría ◽

Felix Duprat ◽

Francisco J. Roa ◽

Nery Jara ◽

Jorge R. Toledo ◽

...

Keyword(s):

Cytotoxic Activity ◽

Cancer Cells ◽

Human Cancer ◽

Ros Generation ◽

Active Components ◽

Total Extract ◽

Human Cancer Cells ◽

Mitochondrial Superoxide ◽

Mitochondrial Membrane Depolarization ◽

Mcf 7

Maytenus disticha (Hook F.), belonging to the Celastraceae family, is an evergreen shrub, native of the central southern mountains of Chile. Previous studies demonstrated that the total extract of M. disticha (MD) has an acetylcholinesterase inhibitory activity along with growth regulatory and insecticidal activities. β-Dihydroagarofurans sesquiterpenes are the most active components in the plant. However, its activity in cancer has not been analyzed yet. Here, we demonstrate that MD has a cytotoxic activity on breast (MCF-7), lung (PC9), and prostate (C4-2B) human cancer cells with an IC50 (µg/mL) of 40, 4.7, and 5 µg/mL, respectively, an increasing Bax/Bcl2 ratio, and inducing a mitochondrial membrane depolarization. The β-dihydroagarofuran-type sesquiterpene (MD-6), dihydromyricetin (MD-9), and dihydromyricetin-3-O-β-glucoside (MD-10) were isolated as the major compounds from MD extracts. From these compounds, only MD-6 showed cytotoxic activity on MCF-7, PC9, and C4-2B with an IC50 of 31.02, 17.58, and 42.19 µM, respectively. Furthermore, the MD-6 increases cell ROS generation, and MD and MD-6 induce a mitochondrial superoxide generation and apoptosis on MCF-7, PC9, and C4-2B, which suggests that the cytotoxic effect of MD is mediated in part by the β-dihydroagarofuran-type that induces apoptosis by a mitochondrial dysfunction.

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