scholarly journals Cytotoxicological evaluation of semi-purified extracts of some dye yielding plants of the Kashmir Valley on Normal Intestinal Cell Line (IEC-6) by MTT assay

2018 ◽  
Vol 7 (1) ◽  
pp. 5-9
Author(s):  
Qazi Gazala ◽  
◽  
Shoukat Ara ◽  
KM Ansari ◽  
Imtiyaz Murtaza ◽  
...  
Keyword(s):  
Cell Line ◽  
Plant Extracts ◽  
Mtt Assay ◽  
Cytotoxic Effect ◽  
Intestinal Cell ◽  
Calendula Officinalis ◽  
Rubia Cordifolia ◽  
Intestinal Cell Line ◽  
Calendula Officinalis L

Plant extracts are widely used in many fields and there is a need to evaluate their cytotoxic effect to determine their non-cytotoxic concentration at which they can be used in a safe manner. Keeping this in view, the present study was designed to evaluate the in vitro toxicity of Celosia argentia L. var plumosa (Cockscomb), Calendula officinalis L. (Pot Marigold), Indigofera heterantha Wall. (Himalayan Indigo) and Rubia cordifolia L. (Indian Madder) on Normal Intestinal Cell Line (IEC-6) by MTT assay to test their feasibility for natural edible dye extraction. The experimental material, comprised of inflorescence of Celosia argentia L. var plumose, petals of the two varieties of Calendula officinalis L., leaves of Indigofera heterantha Wall. and leaves and roots of the Rubia cordifolia L. Cell line was exposed to 1, 4, 16, 64 and 256µg/ml concentrations of plant extracts for 24, 48, and 72hr at 37oC. Results revealed that both the varieties of Calendula officinalis L. var. Gitana Orange and Gitana Yellow did not show any cytotoxic effect on IEC-6 cell line while as Celosia argentia L. var plumose, Indigofera heterantha Wall. and Rubia cordifolia L. showed cytotoxicity. From the present study it was concluded that the extracts of the both varieties of Calendula officinalis L. var. Gitana Orange and Gitana Yellow extracts are non-toxic in nature, thus can be utilized for the extraction of natural edible dye while as the extracts of Celosia argentia L. var plumose, Indigofera heterantha Wall. and Rubia cordifolia L. had potent in vitro cytotoxic activity thus they cannot be used for extraction of natural edible food colour. However, to better evaluate the cytotoxic effect of these plant extracts, in vivo experiments on laboratory animal followed by histological analysis should be done

scholarly journals Preliminary Characterization of the Transcriptional Response of the Porcine Intestinal Cell Line IPEC-J2 to EnterotoxigenicEscherichia coli,Escherichia coli, andE. coliLipopolysaccharide

2010 ◽  
Vol 2010 ◽  
pp. 1-11 ◽  
Author(s):  
Marisa M. Geens ◽  
Theo A. Niewold
Keyword(s):  
Escherichia Coli ◽  
Cell Line ◽  
Transcriptional Response ◽  
Intestinal Cell ◽  
Adhesion Assay ◽  
Transcriptional Level ◽  
Suitable Model ◽  
E Coli ◽  
Intestinal Cell Line

IPEC-J2, a promisingin vitromodel system, is not well characterized especially on the transcriptional level, in contrast to human counterparts. The aim of this study was to characterize the gene expression in IPEC-J2 cells when coincubated with enterotoxigenicEscherichia coli(ETEC), nonpathogenicE. coli, andE. coliendotoxin. Apical infection of polarized IPEC-J2 monolayers caused a time-dependent decrease in transepithelial electrical resistance (TEER). Microarray analysis showed up-regulation of interleukins when IPEC-J2 were cocultured withE. colistrains this has so far never been measured in this cell line. Highest IL8 expression was found with the ETEC strain possessing the F4 fimbrium, suggesting IPEC-J2 cells to be F4 receptor positive, confirmed in a brush border membrane adhesion assay. It is concluded that the innate immune responses to pathogens and LPS makes the IPEC-J2 cell line a suitable model for research on intestinal host pathogen interaction.

scholarly journals Paramylon extracted from Euglena gracilis EOD-1 augmented the expression of SIRT1

2021 ◽  
Author(s):  
Hiromi Ieiri ◽  
Natsuki Kameda ◽  
Junko Naito ◽  
Takanori Kawano ◽  
Norihisa Nishida ◽  
...  
Keyword(s):  
Cell Line ◽  
Microarray Analysis ◽  
Euglena Gracilis ◽  
Epidermal Cells ◽  
Intestinal Cell ◽  
Intestinal Cells ◽  
Target Cells ◽  
Intestinal Cell Line ◽  
Human Intestinal Cell Line

AbstractEuglena gracilis, a type of microalgae, contains several nutrients and accumulates paramylon, a β-1,3-glucan. In recent studies, paramylon has shown to exhibit various activities including immunomoduratory and hepatoprotective effects. In the present study, using an in vitro cell culture system, we aimed to determine whether paramylon derived from the E. gracilis EOD-1 strain, which produces large amounts of paramylon, can augment SIRT1 expression in epidermal cells via activating gut–skin interactions. Results showed that paramylon augmented the expression of SIRT1 in Caco-2 cells, a human intestinal cell line. Furthermore, microarray analysis of Caco-2 cells treated with paramylon showed that paramylon activates epidermal cells through inducing the secretion of factors from intestinal cells. Then, we focused on skin cells as target cells of paramylon-activated intestinal cells. Results showed that secretory factors from Caco-2 cells treated with paramylon augmented the expression of SIRT1 in HaCaT cells, a human keratinocyte cell line, and that expression level of genes related to the growth and maintenance of epidermal cells were significantly changed in Caco-2 cells treated with paramylon as evidenced by microarray analysis. All these results suggest that paramylon can activate epidermal cells by inducing the production of secretory factors from intestinal cells.

Helicobacter pylori Stimulates DNA Synthesis in a Small Intestinal Cell Line in vitro

Digestion ◽  
1998 ◽  
Vol 59 (1) ◽  
pp. 33-39 ◽  
Author(s):  
Johanna Brännström ◽  
Kristina Zachrisson ◽  
Kristina Hultén ◽  
Lars Engstrand ◽  
Andrés Uribe
Keyword(s):  
Helicobacter Pylori ◽  
Dna Synthesis ◽  
Cell Line ◽  
Intestinal Cell ◽  
Intestinal Cell Line ◽  
Small Intestinal

scholarly journals In vitro impact of five pesticides alone or in combination on human intestinal cell line Caco-2

2014 ◽  
Vol 1 ◽  
pp. 474-489 ◽  
Author(s):  
Sylvain Ilboudo ◽  
Edwin Fouche ◽  
Virginie Rizzati ◽  
Adama M. Toé ◽  
Laurence Gamet-Payrastre ◽  
...  
Keyword(s):  
Cell Line ◽  
Intestinal Cell ◽  
Intestinal Cell Line ◽  
Human Intestinal Cell Line
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