scholarly journals Investigation of Borrelia burgdorferi antibodies in patients with multiple sclerosis

2020 ◽  
Vol 11 (2) ◽  
pp. 21-24
Author(s):  
Nilay Ildız ◽  
İbrahim Halil Özerol ◽  
A. Cemal Özcan ◽  
Hamit Çelik
Keyword(s):  
Multiple Sclerosis ◽  
Borrelia Burgdorferi ◽  
Viral Infections ◽  
Antibody Test ◽  
Elisa Test ◽  
Igg Antibodies ◽  
Number Of Patients ◽  
Seroprevalence Data ◽  
Significant Difference ◽  
Antibody Positivity

Background: Lyme is a disease that is non-compulsory in our country and whose seroprevalence data is less studied. Aims and Objective: Recent studies have shown that bacterial and viral infections are risk factor for various neurodegenerative diseases such as multiple sclerosis and Alzheimer’s disease. Herein, we aim to determine the seroprevalence of Lyme in multiple sclerosis (MS) patients. For this purpose, 100 MS patient’s serums were investigated for Borrelia burgdorferi IgM and IgG positivity. Materials and Methods: The results identified with ELISA as positive antibody was confirmed by Western Blot (WB) test. The correlation between ages, gender, occupation, tick history, existence of erythema chronicum migrans (ECM), antibody positivity, pain, year with MS results were investigated using Kolmogorov-Smirnow and Kruskal-Wallis statistical test. Results: B. burgdorferi IgM and IgG antibodies were positive in 8% patients when using ELISA method, but that were found to be 2% by WB. ELISA IgM antibody test gave a 5 negative result in WB. These results were considered false positive in the ELISA test. So, altogether 5 patients were positive by WB method. None of syphilis positive samples detected that B. burgdorferi positive serum. A significant difference between the parameters in terms of IgM positivity was not detected (p> 0.05). B. burgdorferi IgG antibodies were found significant differences between the MS disease duration (p = 0.03). MS in the group of less than 10 years had higher titers of IgG antibodies to B. burgdorferi. Conclusion: Although a small number of patients with MS is positive with Lyme antibodies. Lyme disease is a treatable.Also, If the patient is MS, clinician should be considered Lyme in the differential diagnosis. This is the first study that the correlation between Lyme and MS from Turkey.

scholarly journals Effect of multiple freeze-thaw cycles on detection of anti-SARS-CoV-2 IgG antibodies

2021 ◽  
Author(s):  
Farah M. Shurrab ◽  
Duaa W. Al-Sadeq ◽  
Fathima Amanullah ◽  
Salma N. Younes ◽  
Hadeel Al-Jighefee ◽  
...  
Keyword(s):  
Random Effect ◽  
Elisa Test ◽  
Antibody Detection ◽  
P Value ◽  
Elisa Assay ◽  
Igg Antibodies ◽  
Rna Detection ◽  
Freeze Thaw ◽  
Significant Difference ◽  
Number Of Cycles

AbstractSeveral studies have investigated the effect of repeated freeze-thaw (F/T) cycles on RNA detection for SARS-CoV-2. However, no data is available regarding the effect of repeated F/T cycles on SARS-CoV-2 antibody detection in serum. We investigated the effect of multiple F/T cycles on anti-SARS-CoV-2 IgG detection using an ELISA test targeting the nucleocapsid antibodies. Ten positive and one negative SARS-CoV-2 IgG sera from 11 participants, in replicates of five were subjected to a total of 16 F/T cycles and stored at 4°C until tested by ELISA. Statistical analysis was done to test for F/T cycle effect. Non-of the 10 positive sera turned into negative after 16 F/T cycles. There was no significant difference in the OD average reading between the first and last F/T cycles, except for one serum with a minimal decline in the OD. The random-effect linear regression of log (OD) on the number of cycles showed no significant trend with a slope consistent with zero (B=-0.0001; 95% CI −0.0008; 0.0006; p-value=0.781). These results suggest that multiple F/T cycles had no effect on the ability of the ELISA assay to detect the SARS-CoV-2 IgG antibodies.

scholarly journals Paramyxoviruses and Multiple Sclerosis

2012 ◽  
Vol 9 (3) ◽  
pp. 500-503
Author(s):  
Baghdad Science Journal
Keyword(s):  
Multiple Sclerosis ◽  
Autoimmune Disease ◽  
Environmental Factors ◽  
Neurological Disease ◽  
Epidemiological Data ◽  
Igg Antibodies ◽  
Significant Difference ◽  
Elisa Technique

Multiple Sclerosis (MS) is a progressive neurological disease characterized by periods of quiescence and exacerbation, epidemiological data suggest the notion that MS is an acquired autoimmune disease caused by environmental factors, probably infectious, in genetically susceptible individuals.The submitted research was attempted to study the possible viral (Paramyxoviruses) role in MS, the sera of 57 MS patients were assayed for anti-measles and anti-mumps IgG antibodies using ELISA technique, the results were compared in order to establish the presence or absence of a significant difference regarding both number of positive cases and antibodies titer between the two groups, the results revealed that there is no in number of measles positive cases in both MS patients and controls while a significant difference in number of positive cases of mumps and measles anti-measles IgG titer and a highly significant difference regarding mumps IgG Abs titer between the two studied groups, this may be considered as a preliminary indicator to the role of those two paramyxoviruses in MS.

The Relationship between Systemic Anticoagulation during Cardiac Surgery and Heparin Platelet Factor 4 Antibody Formation.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 3951-3951
Author(s):  
John L. Francis ◽  
Alane Drexler ◽  
Jamie Walker ◽  
Kristin Rathmann
Keyword(s):  
Cardiac Surgery ◽  
Antibody Formation ◽  
Antibody Test ◽  
Platelet Factor 4 ◽  
Serum Samples ◽  
Platelet Factor ◽  
Off Pump ◽  
Number Of Patients ◽  
Antibody Positivity ◽  
Systemic Anticoagulation

Abstract Heparin-induced thrombocytopenia occurs in approximately 1–3% of patients receiving unfractionated heparin. However, up to 50% of patients receiving heparin for systemic anticoagulation during cardiac surgery with cardiopulmonary bypass develop antibodies to the heparin-platelet factor 4 (HPF4) complex detectable by enzyme-linked immunoassays (ELISA). This high incidence of seroconversion is not diminished by off-pump cardiac surgery (OPCAB), despite significantly lower doses of heparin used for surgical anticoagulation. We tested the hypothesis that HPF4 antibodies develop following cardiac surgery even when heparin is not used for surgical anticoagulation. Patients (n=133) were randomized (2:1) to receive bivalirudin (The Medicines Company, Parsippany, NJ) or heparin for surgical anticoagulation during OPCAB. There were no restrictions on the pre-operative or post operative use of heparin. Blood samples were obtained pre-operatively (baseline), and 7 and 30 days after surgery. Serum samples were stored frozen until tested with a commercially available ELISA for HPF4 antibodies (GTI Inc., WI). An optical density (OD) ≥ 0.4 was considered positive, while an OD >1.0 was deemed strongly positive. The percentage of patients having a positive HPF4 antibody test (OD ≥ 0.4), was similar at baseline (6.7 vs. 6.8%) and at 7 days (27.1 vs. 29.3%) in bivalirudin and heparin-treated patients, respectively. At 30 days, the frequency of HPF4 antibodies was lower in the patients who received bivalirudin for surgical anticoagulation (32.5% vs. 40.9%). The percentage of patients having a strongly positive ELISA (OD > 1.0) was 0% at baseline in both groups, 4.3 vs. 7.3% at 7 days and 4.8 vs. 9.1% at 30 days for bivalirudin vs heparin, respectively. These data suggest that HPF4 antibody formation during hospitalization for cardiac surgery is not dependent only on the heparin used for anticoagulation during surgery. Antibody positivity in baseline samples presumably results from pre-operative heparin use and is consistent with previous reports. Since bivalirudin is not capable of eliciting an HPF4 antibody response, post-operative seroconversion presumably reflects a combination of both pre- and post-operative heparin use. However, when heparin was used for surgical anticoagulation, the number of patients subsequently developing strongly positive HPF4 antibodies was almost twice that seen in the bivalirudin group. This suggests that the use of bivalirudin as a surgical anticoagulant may reduce the incidence and risk of HIT following cardiac surgery.

scholarly journals Highly Sensitive and Specific SARS-CoV-2 Serological Assay Using a Magnetic Modulation Biosensing System

Biosensors ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 7
Author(s):  
Shira Avivi-Mintz ◽  
Yaniv Lustig ◽  
Victoria Indenbaum ◽  
Eli Schwartz ◽  
Amos Danielli
Keyword(s):  
Viral Infections ◽  
Limit Of Detection ◽  
Turnaround Time ◽  
Elisa Test ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Serological Assay ◽  
Clinical Sensitivity ◽  
Highly Sensitive

Sensitive serological assays are needed to provide valuable information about acute and past viral infections. For example, detection of anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) IgG antibodies could serve as the basis for an “immunity passport” that would enable individuals to travel internationally. Here, utilizing a novel Magnetic Modulation Biosensing (MMB) system and the receptor-binding domain of the SARS-CoV-2 spike protein, we demonstrate a highly sensitive and specific anti-SARS-CoV-2 IgG serological assay. Using anti-SARS-CoV-2 IgG antibodies, RT-qPCR SARS-CoV-2-positive and healthy patients’ samples, and vaccinees’ samples, we compare the MMB-based SARS-CoV-2 IgG assay’s analytical and clinical sensitivities to those of the enzyme-linked immunosorbent assay (ELISA). Compared with ELISA, the MMB-based assay has an ~6-fold lower limit of detection (129 ng/L vs. 817 ng/L), and it detects an increase in the IgG concentration much earlier after vaccination. Using 85 RT-qPCR SARS-CoV-2-positive samples and 79 -negative samples, the MMB-based assay demonstrated similar clinical specificity (98% vs. 99%) and sensitivity (93% vs. 92%) to the ELISA test, but with a much faster turnaround time (45 min vs. 245 min). The high analytical and clinical sensitivity, short turnaround time, and simplicity of the MMB-based assay makes it a preferred method for antibody detection.

scholarly journals Impact of supplementation with "multivitamin-mineral" specially formulated to improve fatigue and inflammatory state in patients with multiple sclerosis: A triple-blind, randomized, placebo-controlled trial

2021 ◽  
Author(s):  
Sama Bitarafan ◽  
Elmira Karimi ◽  
Abdorreza Naser Moghadasi ◽  
Razieh Sadat K Kazemi-Mozdabadi ◽  
Zinat Mohammadpour ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Controlled Trial ◽  
Intervention Group ◽  
Complementary Therapy ◽  
Interleukin 4 ◽  
Control Group ◽  
Fatigue Score ◽  
Number Of Patients ◽  
Significant Difference ◽  
Inflammatory State

Background: Multiple sclerosis (MS) is an inflammatory disease of the central nervous system (CNS) with the most common complaint of fatigue. A high number of patients with MS are interested in taking dietary supplements as a complementary therapy. We propose a specially formulated supplement for patients with MS and aim to evaluate its effects on fatigue. Methods: This study was a triple-blind, randomized, placebo-controlled trial using a stratified randomization method according to sex. 46 eligible patients participated in the study, 23 in the placebo group and 23 in the intervention group. The intervention group received two capsules of multivitamin-mineral (MVM) daily for 3 months. Measurements of fatigue and cytokines were performed in all patients at the baseline and after the 3-month intervention Results: Finally, information of 41 participants was used for data analysis. However, fatigue was decreased after supplementation than before, in the intervention group (P = 0.005). There was no significant difference (P = 0.090) between the change of fatigue score in the MVM group (-3.00 ± 4.42) and the control group (-0.40 ± 5.14). Among cytokines, Interleukin 4 (IL-4) significantly increased in the intervention group compared to the placebo (P = 0.030). Conclusion: Our study showed that the present MVM probably could improve the inflammatory state and fatigue in patients with MS.

scholarly journals Serology characteristics of SARS-CoV-2 infection after exposure and post-symptom onset

2020 ◽  
Vol 56 (2) ◽  
pp. 2000763 ◽  
Author(s):  
Bin Lou ◽  
Ting-Dong Li ◽  
Shu-Fa Zheng ◽  
Ying-Ying Su ◽  
Zhi-Yong Li ◽  
...  
Keyword(s):  
Early Stage ◽  
Antibody Test ◽  
Igg Antibodies ◽  
Specific Diagnosis ◽  
Treatment And Prevention ◽  
Significant Difference ◽  
Antibody Levels ◽  
Antibody Dynamics ◽  
Serology Testing ◽  
Post Onset

BackgroundTimely diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is a prerequisite for treatment and prevention. The serology characteristics and complement diagnosis value of the antibody test to RNA test need to be demonstrated.MethodSerial sera of 80 patients with PCR-confirmed coronavirus disease 2019 (COVID-19) were collected at the First Affiliated Hospital of Zhejiang University, Hangzhou, China. Total antibody (Ab), IgM and IgG antibodies against SARS-CoV-2 were detected, and the antibody dynamics during the infection were described.ResultsThe seroconversion rates for Ab, IgM and IgG were 98.8%, 93.8% and 93.8%, respectively. The first detectible serology marker was Ab, followed by IgM and IgG, with a median seroconversion time of 15, 18 and 20 days post exposure (d.p.e.) or 9, 10 and 12 days post onset (d.p.o.), respectively. The antibody levels increased rapidly beginning at 6 d.p.o. and were accompanied by a decline in viral load. For patients in the early stage of illness (0–7 d.p.o), Ab showed the highest sensitivity (64.1%) compared with IgM and IgG (33.3% for both; p<0.001). The sensitivities of Ab, IgM and IgG increased to 100%, 96.7% and 93.3%, respectively, 2 weeks later. When the same antibody type was detected, no significant difference was observed between enzyme-linked immunosorbent assays and other forms of immunoassays.ConclusionsA typical acute antibody response is induced during SARS-CoV-2 infection. Serology testing provides an important complement to RNA testing in the later stages of illness for pathogenic-specific diagnosis and helpful information to evaluate the adapted immunity status of patients.

111 Epileptic seizures in multiple sclerosis

2018 ◽  
Vol 89 (6) ◽  
pp. A44.1-A44
Author(s):  
Stephen Walsh ◽  
Joel Corbett ◽  
K Meng Tan ◽  
Simon Broadley
Keyword(s):  
Multiple Sclerosis ◽  
Demyelinating Disease ◽  
Age Of Onset ◽  
Case Reports ◽  
Case Series ◽  
Clinical Information ◽  
Epileptic Seizures ◽  
Disease Course ◽  
Number Of Patients ◽  
Significant Difference

IntroductionEpileptic seizures have been described in association with multiple sclerosis (MS) in both anecdotal case reports and case series. The recent identification of specific antibodies to myelin oligodendrocyte glycoprotein (MOG) protein in a small number of patients with demyelinating disease which may resemble neuromyelitis optica or acute disseminated encephalopathy, which may involve seizures, raises the possibility that anti-MOG antibody related demyelination may account for the association of epilepsy with MS.MethodsWe have undertaken a retrospective review of cases of MS diagnosed at the Gold Coast MS clinic over a 10 year period. All cases were systematically asked if they had ever had an epileptic seizure either via a patient completed questionnaire or at a clinic visit. Demographic and clinical information were also recorded. These data have been analysed using descriptive statistics and appropriate tests for significant differences between those with epilepsy and those without.Results428 cases with complete data were identified. Those with a history of epilepsy were slightly younger (median (range); 44.5 (27–64) years vs 4715–88 years), but this difference was not statistically significantly different. The gender ratio was the same for both groups (9/12 (75%) for those with epilepsy and 326/416 (78%)). There was no significant difference in age of onset, disease course, relapse frequency or level of disability. Although numbers are small, seizure appear to occur most frequently earlier in the disease course and are rarely an ongoing issue.ConclusionThese data support earlier work indicating that epilepsy occurs in people with MS who are younger. This fits with the notion that seizures arise in the context of the inflammatory stage of multiple sclerosis rather than the degenerative phase. Further work needs to be undertaken to assess any association with anti-MOG antibodies and epileptic seizures in demyelinating disease.

scholarly journals Detection of anti-Borrelia burgdorferi antibodies in buffaloes (Bubalus bubalis) in the state of Pará, Brazil

2012 ◽  
Vol 21 (3) ◽  
pp. 338-341 ◽  
Author(s):  
Fabíola do Nascimento Corrêa ◽  
Rafaella Câmara Teixeira ◽  
Carlos Magno Chaves Oliveira ◽  
José Diomedes Barbosa ◽  
Adivaldo Henrique da Fonseca
Keyword(s):  
Borrelia Burgdorferi ◽  
North American ◽  
Elisa Test ◽  
The State ◽  
Boophilus Microplus ◽  
Serum Samples ◽  
North American Strain ◽  
The North ◽  
Significant Difference ◽  
American Strain

This study aimed to investigate the frequency of homologous antibodies of IgG class against Borrelia burgdorferi in buffaloes in the state of Pará, Brazil. Blood serum samples from 491 buffaloes were analyzed by means of the indirect ELISA test, using crude antigen produced from a cultivar of the North American strain G39/40 of B. burgdorferi. There were 412 positive samples (83.91%), and there was no statistically significant difference in the proportions of positive animals between the 81.69% (232/284) originating from Marajó Island and the 86.96% (180/207) from the continental area of the state of Pará. In all the municipalities studied, the frequency of positive findings of antibodies against B. burgdorferi among the animals ranged from 63.6% to 92.9%. The high numbers of seropositive animals can be explained by the frequent presence of the tick Rhipicephalus (Boophilus)microplus, and by the possible existence of spirochetes of the genus Borrelia infecting buffaloes in the region studied, although specific studies are needed to confirm this relationship. These factors suggest that a cross-reaction exists between the North American strain G39/40 of B. burgdorferi, which is used as an antigenic substrate, and the species of Borrelia spp. that possibly infects buffaloes in the state of Pará.

scholarly journals A Controlled Trial of Mitoxantrone in Multiple Sclerosis: Serial MRI Evaluation at One Year

1994 ◽  
Vol 21 (3) ◽  
pp. 266-270 ◽  
Author(s):  
S. Bastianello ◽  
C. Pozzilli ◽  
F. D’Andrea ◽  
E. Millefiorini ◽  
M. Trojano ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Gadolinium Dtpa ◽  
Treatment Groups ◽  
Number Of Patients ◽  
Significant Difference ◽  
Relapsing Remitting ◽  
The Mean ◽  
Remitting Multiple Sclerosis ◽  
One Year ◽  
Relapsing Remitting Multiple Sclerosis

Abstract:We present the results of a randomized double-blinded placebo controlled, multicenter trial, of low-dose mitoxantrone (MX), after one year, in 25 patients with relapsing-remitting multiple sclerosis, who had serial enhanced magnetic resonance imaging (MRI). Treatment groups were balanced for age, gender, duration of illness and neurological disability. Five of the 13 MX patients and 10 of the 12 placebo patients had exacerbations during treatment (p < 0.02). The mean change in the extended disability status scale was not significantly different between the MX and placebo treatment groups. Serial Gadolinium-DTPA enhancedMRIdetected no significant difference between the MX treated and placebo groups in the mean total number of new, enlarging, or Gadolinium-DTPA enhancing lesions; there was a trend toward a reduction of new, enlarging and Gadolinium-DTPA enhancing lesions in MX patients. Despite this ameliorating effect, the results indicate that serial Gadolinium-DTPA enhanced MRI, performed over one year in a limited number of patients, could not provide conclusive evidence for a role of MX therapy in relapsing-remitting multiple sclerosis.

scholarly journals Neospora spp. antibodies in horses from two geographical regions of the state of Santa Catarina, Brazil

2013 ◽  
Vol 22 (4) ◽  
pp. 597-601 ◽  
Author(s):  
Anderson Barbosa de Moura ◽  
Márcio Orides da Silva ◽  
Juliana Antunes Farias ◽  
Achilles Vieira-Neto ◽  
Antonio Pereira de Souza ◽  
...  
Keyword(s):  
Coastal Region ◽  
Antibody Test ◽  
The State ◽  
Mountain Region ◽  
Southern Brazil ◽  
Igg Antibodies ◽  
Santa Catarina ◽  
Geographical Regions ◽  
Significant Difference ◽  
Immunofluorescent Antibody Test

The aims of this study were to determine occurrences ofNeospora spp. IgG antibodies in horses from two geographical regions of the state of Santa Catarina, southern Brazil, and identify risk factors for infection. Analyses were performed on 615 samples using the immunofluorescent antibody test (IFAT ≥ 1:50). Out of the 615 samples, 25 (4.1%) were positive for Neospora spp. The titers for Neospora spp. were distributed as follows: 1:50 (13), 1:100 (eight), 1:200 (three) and 1:400 (one). Out of the 311 samples taken in the mountain region, eight were positive (2.6%). Among the samples from the coastal region (304), 17 had Neospora spp. antibodies, thus indicating occurrence of 5.6%. Although no statistically significant difference was observed (P = 0.06704), the prevalence among animals of the coast was 2.2 times higher than that of the mountain region. Contact with dogs and/or cattle (P = 0.007596) were identified as risk factor forNeospora spp. infection.

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