scholarly journals Evaluation of cultivars and land races of Oryza sativa for restoring and maintaining wild abortive cytoplasm

1970 ◽  
Vol 1 (2) ◽  
pp. 87-91
Author(s):  
Bal K Joshi ◽  
Laxmi P Subedi ◽  
Santa B Gurung ◽  
Ram C Sharma
Keyword(s):  
Growing Season ◽  
Pollen Sterility ◽  
Pedigree Analysis ◽  
Spikelet Fertility ◽  
Male Sterile ◽  
Animal Science ◽  
F1 Hybrid ◽  
Cms Line ◽  
Hybrid Seeds ◽  
Wild Abortive Cytoplasm

Identification of restorers and maintainers from cultivars and landraces through test crossing and their use in further breeding programme are the initial steps in three-line heterosis breeding. Two experiments, one in the greenhouse for F1 hybrid seeds production and another in the field for parental screening, were conducted during the 1999 rice growing season at the Institute of Agriculture and Animal Science (IAAS, TU), Rampur, Nepal. Three cytoplasmic male sterile (CMS) lines, eight improved cultivars and six landraces of rice were studied for their fertility restoring and sterility maintaining abilities. Pollen sterility was studied based on their stainability with potassium iodide iodine (I-KI) solution. On the basis of their interaction with I-KI, pollens were categorized as unstained withered sterile (UWS), unstained spherical sterile (USS), stained round sterile (SRS) and stained round fertile (SRF). For each hybrid, the percentage of spikelet fertility was estimated. The test lines were categorized as restorers, partial restorers, maintainers, and partial maintainers on the basis of pollen sterility and spikelet fertility. The male sterile lines had mostly UWS and USS types of pollen, whereas the restorer lines had more SRS and SRF types. There was no strong evidence for a relationship between pollen fertility and spikelet fertility. Five restorers, three partial restorers, two partial maintainers and four maintainers were identified. These restorers can be used to develop the hybrid seed while maintainers to maintain and/or to develop new CMS lines, because these are locally adapted cultivars. Pedigree analysis revealed that, for some of these test lines, TN-1 and CR94-13 might be the donors of maintainer and restorer gene(s), respectively. Key words: CMS line, maintainer, restorer, rice Himalayan Journal of Sciences 1(2): 87-91, 2003

scholarly journals Pollen and Spikelet Analysis in F1 Rice Hybrids and their Parents

2014 ◽  
Vol 8 ◽  
pp. 120-126 ◽  
Author(s):  
Bal K. Joshi ◽  
Laxmi P. Subedi ◽  
Santa B. Gurung ◽  
Ram C. Sharma
Keyword(s):  
Pollen Fertility ◽  
Practical Importance ◽  
Pollen Grains ◽  
Gene Interaction ◽  
Pollen Sterility ◽  
Spikelet Fertility ◽  
High Fertility ◽  
Male Sterile ◽  
Pollen Abortion ◽  
Spikelet Sterility

Pollen analysis can be used to discriminate between different species, identify possible  interspecies hybrids, identify restorer and maintainer lines, useful to study genetics of  restorer gene, interaction between chromosome and cytoplasm and relationship between  parents. Pollen abortion system of male sterility is an important tool in hybrid rice  production and spikelet is the major yield components. Nine improved cultivars, six  landraces and three wild aborted cytoplasmic-genetic male sterile (CMS) lines were used to  analyze pollen and spikelet in F1 rice hybrids and their parents. The frequency of pollen  categories and its relationship to spikelet fertility were investigated. Pollen sterility of the  F1s was determined by staining pollen grains in 1% potassium iodide-iodine (I-KI) solution.  Spikelet fertility was determined by counting the total number of seed set in proportion to  the total number of spikelets. Correlation and regression coefficients for some traits were  computed. In hybrids, pollen fertility ranged from 0.5 to 82% and spikelet fertility from 0 to  87%. Pollen fertility varied from 28 to 97%, while spikelet fertility from 73 to 91% in pollen  parents. The highest and the lowest percentages of pollen fertility were found in Chaite-6  and Chiunde cultivars respectively. Spikelet fertility percentage varied widely among  hybrids and many hybrids had lower spikelet fertility percentage than their parents.  Therefore, it is of practical importance to understand the causes of high spikelet sterility in  hybrids for possible increase in spikelet fertility. Highly significant positive correlation was  found between stained round fertile (SRF) pollen and spikelet fertility. The positive value of  correlation and regression coefficient on SRF and spikelet fertility were found between F1  and mid parent, and F1 and male parent. High fertility of cross may be due to the presence of  a wide compatibility gene or restorer genes in the cultivar. Lower pollen and spikelet  fertility of the crosses was attributed to effect of the genetic background of the tester parent. Nepal Agric. Res. J. Vol. 8, 2007, pp. 120-126 DOI: http://dx.doi.org/10.3126/narj.v8i0.11605  

scholarly journals Barley CMS detected in Finland in 1976 enabled growing of productive winter-barley F1 hybrids in the European winter-barley zone since 2002

2018 ◽  
Author(s):  
Hannu Ahokas
Keyword(s):  
Winter Barley ◽  
F1 Hybrids ◽  
Cytokinin Activity ◽  
Male Sterile ◽  
Lodging Resistance ◽  
F1 Hybrid ◽  
Alopecurus Myosuroides ◽  
Restorer Genes ◽  
Hybrid Seeds ◽  
The Uk

My wide crossing program of barley (Hordeum vulgare s.l.) in 1976 yielded a system which could be used to produce F1 hybrid seeds. The genotypes were designated as msm1 (male sterile, maternal), and Rfm1a (restorer of fertility in msm1). I later found 19 other strains with dominant restorer alleles, which were carriers of a fertile cytoplasm. Hence, the restorer genes probably evolved in advance creating an opportunity for the cytoplasm to mutate to male sterility. Cytological studies revealed an uncontrolled secretion of sporopollenin in the sterile anthers, leading to their starvation and sterility. The Rfm1a gene was shown to cause an increase in the cytokinin activity of Fraction 7 in the root sap of barley, regardless of the cytoplasm type. In 1980, I found another male sterile cytoplasm, msm2, whose anther can also be restored by the Rfm1 alleles. The msm2 strain originally had complementary partial restorer genes and was found to be more responsive to such restorers than msm1. In Germany, the Rfm1 gene was recently translocated to a rye (Secale cereale) chromosome to study its response in CMS rye. The msm2 cytoplasm could be distinguished from msm1 with electron microscopy at the early stages of the anthers. The msm1 cytoplasm is not known to be associated with increased disease susceptibility, unlike the T-sterile cytoplasm formerly used to produce hybrid seeds of maize (Zea mays). Hybrid cvs: Seeds of msm1–Rfm1a were first requested from me by Hilleshög AB in Sweden. Hilleshög later became a part of Syngenta. Hilleshög techniques for sugar-beet hybrids were applied by Syngenta breeders to produce hybrid barley seeds. Syngenta introduced the first commercial winter-barley hybrid in the UK in 2002. Their hybrid cvs were marketed to countries growing winter-barley in Europe. The ha yields of their hybrids exceeded those of conventional cultivars or parental lines by about 1000 kg. In Spain, the winter-barley hybrid yielded 21 percent more than the conventional cultivars in 2015, when grown in the field scale. For the 2016 harvest, hybrids were sown in Germany on more than 140000 ha, which reflects 11.6 percent of the total feed barley area. In 2017, Syngenta launched a cashback scheme for, if their hybrid cultivars did not comfortably out-yield the farmer's conventional counterpart that season. Hybrid seeds must be acquired for each sowing. Unlike conventional monogenic barley cultivars, the hybrids exploit genetic variability and heterozygosity. Hybrid winter-barley is the most competitive of winter cereals with the aggressive weed Alopecurus myosuroides in the UK. Thick stems in new hybrids increase lodging resistance. The winter-barley hybrid ‘Wootan’ gave ha yields up to 6000 kg in Tammisaari, though incompletely winter-hardy in Finland. Maturing a month later than the hybrid ‘Hobbit’ and with optimal winterhardiness, winter-barley hybrids could exceed ha yields of 10000 kg in Finland. Some other breeding companies seem to work for hybrid barley, too.

scholarly journals Identification of maintainer and restorer lines in local aromatic Rice (Oryza sativa)

2014 ◽  
Vol 39 (1) ◽  
pp. 1-12
Author(s):  
M Ali ◽  
MA Hossain ◽  
MJ Hasan ◽  
ME Kabir
Keyword(s):  
Oryza Sativa ◽  
Cytoplasmic Male Sterility ◽  
Pollen Sterility ◽  
Spikelet Fertility ◽  
Aromatic Rice ◽  
Commercial Exploitation ◽  
Fertility Status ◽  
Cms Line ◽  
Single Cross ◽  
Rice Genotypes

The availability of stable cytoplasmic male sterility and fertility restoring system is vital for commercial exploitation of heterosis in rice. The experiment was conducted to identify stable maintainers and restorers for three CMS lines having wild abortive type sterility inducing genes in local rice germplasm. One hundred and twenty nine test crosses were made by using 43 aromatic rice genotypes and three CMS lines. Pollen sterility and spikelet fertility of the raised F1s were assessed in consecutive 2006-2007 Boro and 2007 T. Aman seasons. Pollen sterility was categorized as 100% sterility for complete sterile (CS), 91- 99% as sterile (S), 71-90% as partial sterile (PS), 31-70% as partial fertile (PF), 21-30% as fertile (F), and 0-20% sterility as full fertile (FF). Pollen sterility of F1s in Boro 2006-07 season ranged from 18.83 to 100%, while in T. Aman 2007 season, it ranged from 15.28 to 100%. Whereas spikelet fertility in Boro 2006- 07 season ranged from 0.86 to 69.71% and in T. Aman 2007 season, it ranged from 1.97 to 82.91% with CMS line IR58025A. Out of 43 F1s with IR58025A, two were found complete sterile (IR58025A/Kaliijira-9 and IR58025A/ Sorukamini-2) and three as full fertile (IR58025A/Agali, IR58025A/Benaful, and IR58025A/Khasa). With CMS line IR62829A, pollen sterility ranged from 2.77 to 100% in Boro 2006-07 season and 0.96 to 100% in T. Aman 2007 season. Spikelet fertility status of the same combinations ranged from 0 to 75.41% in Boro 2006-07 season and 1.76 to 88.36% in T. Aman season 2007. Six crosses were identified as complete sterile and seven as full fertile for the said combination. In case of IR68885A four crosses were found as complete sterile, and a single cross as full fertile. The local aromatic line, Kalijira-9 and Sorukamini were identified as maintainer for IR68885A which was common with IR58025A. The local aromatic line Benaful was identified as restorer for IR58025A and IR62829A. DOI: http://dx.doi.org/10.3329/bjar.v39i1.20056 Bangladesh J. Agril. Res. 39(1): 1-12, March 2014

scholarly journals Development of cytoplasmic male sterile lines and restorer lines of various elite Indica Group rice cultivars using CW-CMS/Rf17 system

Rice ◽  
2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Kinya Toriyama ◽  
Tomohiko Kazama ◽  
Tadashi Sato ◽  
Yoshimichi Fukuta ◽  
Masaaki Oka
Keyword(s):  
Nuclear Gene ◽  
Oryza Rufipogon ◽  
Rice Cultivars ◽  
Male Sterile ◽  
Cytoplasmic Male Sterile ◽  
Cms Line

Abstract Background A cytoplasm of CW-type cytoplasmic male sterile (CMS) line is derived from Oryza rufipogon strain W1 and fertility is restored by a single nuclear gene, Rf17. We have previously reported that CW-CMS were effective for breeding CMS lines of Indica Group rice cultivars, IR 24 and IR 64. The applicability of this CW-CMS/Rf17 system to produce other elite Indica Group rice cultivars with CMS was explored. Findings Out of seven elite Indica Group rice cultivars, complete CMS lines were obtained for six cultivars: NSIC Rc 160, NSIC Rc 240, Ciherang, BRRI dhan 29, NERICA-L-19, and Pusa Basmati. The fertility of these six lines was restored when Rf17 was present. A CMS line was not obtained for the cultivar Samba Mahsuri. Conclusions The CW-CMS/Rf17 system will be useful to produce CMS lines and restorer lines of various elite Indica Group rice cultivars.

scholarly journals Heterotic Effect of Different Cytoplasmic Combinations in Sunflower Hybrids Cultivated Under Diverse Irrigation Regimes

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 465 ◽  
Author(s):  
Vikrant Tyagi ◽  
Satwinder Kaur Dhillon ◽  
Gurpreet Kaur ◽  
Prashant Kaushik
Keyword(s):  
Oleic Acid ◽  
Water Stress ◽  
Oil Content ◽  
Male Sterile ◽  
Biochemical Traits ◽  
F1 Hybrid ◽  
Cytoplasmic Diversity ◽  
Days To Maturity ◽  
Number Of Leaves ◽  
Different Sources

The sunflower hybrids hold a narrow cytoplasmic diversity. Besides, the heterotic effect of wild cytoplasmic combinations of sunflower on important traits under water stress has not been explored in detail. Here, we evaluated the different sunflower cytoplasmic combinations in sunflower hybrids using cytoplasmic male sterile (CMS) sources as female parents. We used a total of sixteen sunflower genotypes representing twelve CMS lines from wild and conventional sources along with four restorer lines. Twelve CMS lines were crossed with four restorer lines to develop a total of 48 F1 hybrid combinations. The hybrids were evaluated under two different environments (i.e., regular irrigation and water stress) for morphophysiological, yield, and biochemical traits over two years. Heterotic effect for various CMS sources was evaluated on all of the three possible scales, namely, better-parent heterosis (BPH), mid-parent heterosis (MPH), and heterosis as percent of check (PSH-996). For better-parent and mid-parent heterosis, the CMS sources Helianthus annuus, Helianthus argophyllus, and Helianthus debilis demonstrated positive better-parent heterosis for seed yield, oil content, and oleic acid irrespective of the environment. However, the hybrid combinations of different sources when using the genotype RCR8297 as the restorer parent recorded maximum average returns. Furthermore, chlorophyll meter (SPAD) reading positively correlated with days to 50% flowering, days to maturity, plant height, and number of leaves per plant in both the environments. Overall, this study identified and compared the heterotic effect of the different cytoplasmic combinations in sunflower under water stress as well as under normal irrigation environments.

Cytoplasmic male sterility in Desmodium

1969 ◽  
Vol 20 (2) ◽  
pp. 227 ◽  
Author(s):  
KS McWhirter
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Interspecific Hybrid ◽  
Pollen Fertility ◽  
Pollen Sterility ◽  
Hybrid Origin ◽  
F1 Hybrids ◽  
Male Sterile ◽  
Genetic Stocks

A type of male sterility found in two Desmodium plants of probably interspecific hybrid origin was cytoplasmically inherited. The cytoplasmic male-sterile character was incorporated in the tropical legume Desmodium sandwicense by backcrossing. In this genetic background pollen sterility was complete. The male-sterile character was not graft-transmissible, and it produced no detectable pleiotropic effects on growth and development. Desmodium intortum gave restoration of pollen fertility in Fl hybrids with male-sterile lines of D. sandwicense. Restored F1 hybrids produced apparently normal pollen, but tests of functional ability of the pollen disclosed that pollen fertility was less than that of Fl hybrids with normal cytoplasm. Incomplete restoration of fertility was not due to heterozygosity of fertility-restoring genes with gametophytic expression, since fertility-restoring genes were shown to act sporophytically. The results established the occurrence in the legume Desmodium of a system of determination of the male-sterile, fertility-restored phenotypes that is similar to the cytoplasmic male sterility systems described in many other angiosperm plants. A scheme utilizing the genetic stocks produced in this study for commercial production of the interspecific hybrid D. sandwicense x D. intortum as a cultivar is presented.

Low temperature induced male sterility in Sorghum bicolor

1971 ◽  
Vol 11 (50) ◽  
pp. 352 ◽  
Author(s):  
RW Downes ◽  
DR Marshall
Keyword(s):  
Low Temperature ◽  
Male Sterility ◽  
Temperature Regime ◽  
Flag Leaf ◽  
Pollen Sterility ◽  
Male Sterile ◽  
Low Degree ◽  
The Mean ◽  
Mother Cells ◽  
Breeding And Genetics

Male sterility was induced in sorghum by exposing plants to a temperature regime of 18/13�C (day-night temperatures) during meiosis in the pollen mother cells. This normally occurs at the time the last (flag) leaf is emerging and elongating. The majority of genotypes examined were rendered completely male sterile by the low temperature regime. However, some genotypes retained a low degree of pollen fertility. The low temperatures appeared to have little, if any, effect on female fertility. The available evidence indicates that it is the night temperature, rather than the mean temperature, which is critical for the induction of pollen sterility. The potential uses of this method of inducing male sterility in plant breeding and genetics programs are briefly discussed.

Genetic purity analysis of hybrid broccoli (Brassica oleracea var. italica) seeds using RAPD PCR

2002 ◽  
Vol 53 (1) ◽  
pp. 51 ◽  
Author(s):  
Phillip A. Crockett ◽  
Mohan B. Singh ◽  
C. K. Lee ◽  
Prem L Bhalla
Keyword(s):  
Plant Size ◽  
Vegetable Crops ◽  
Genetic Purity ◽  
F1 Hybrid ◽  
Shoot Ratio ◽  
Root Shoot Ratio ◽  
Rapd Pcr ◽  
Purity Analysis ◽  
Hybrid Seeds ◽  
Contrast Response

Determination of genetic purity of F1-hybrid seeds is aquality control requirement in the production of hybrid Brassica vegetableseeds. Hybrid varieties of these vegetable crops have arisen from a limitedgermplasm base, making discrimination of parental and hybrid lines verylaborious and troublesome. The use of RAPD PCR for evaluating seed purity in acommercial F1-hybrid broccoli a single cultivar bywithholding water. In Expt 2, plants of EP and MK were grown together in thesame container and received water daily with gradation in intensity of waterdeficit achieved by varying the daily water ration per container.All cultivars in each experiment exhibited commonly reported responses towater deficit, characterised by diminished evaporative surface area andincreased root : shoot ratio. The response of MK was primarily morphologicaland MK plants had smaller plant size, higher root : shoot ratio, and a lowergrowth rate compared with temperate cultivars. By contrast, response oftemperate cultivars was primarily physiological; stomatal conductance oftemperate cultivars was lower and these cultivars had a greater tendency forleaf lamina osmotic a

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