Identification and Characterization of Panama Wilt Causing Fungal Isolates to Musa Paradisiaca cv. Puttabale

Musa paradisiaca L. (Musaceae) cultivar ‘Puttabale’ is an indigenous banana commonly cultivated in the farmyards of Chitradurga,Davanagere, Chikmagalur and Shivamogga district of Karnataka. The fruits are valued for delicious taste. This banana cultivar is highlysusceptible to Fusarium infection and the yield loss has estimated to 30-40% at standing stage. The corm tissue of Musa paradisiaca cv.Puttabale showing typical panama wilt symptoms were collected from pathogen infected banana farmyard of Bhadravati, Sagara, Shikaripura,Holehonnur, Soraba, Thirthahalli, Gonibeedu and Lakkavalli regions of Shivamogga district of Karnataka. These infected corms were cultureon PDA medium and examined carefully by morphological and molecular identification. Out of the eight fungal isolates, the colonies of fungalisolates collected from Bhadravati, Sagara, Holehonnur, Thirthahalli, Gonibeedu and Lakkavalli regions produced a dense white aerialmycelium that evenly spread on the growth medium similar to the colony texture of Fusarium species. Among these fungal isolates, thepathogen collected from Gonibeedu region showed the presence of sickle or rod shaped, four-celled macro conidiophores, round or ovalshaped chlamydospores and the BLASTn search result showed 99 % similarity with the sequences of Fusarium oxysporum f. sp. cubense(Foc). The only one fungal isolate showed the presence of actual panama wilt causing fungi.DOI: http://dx.doi.org/10.3126/ijasbt.v2i4.11135 Int J Appl Sci Biotechnol, Vol. 2(4): 420-425

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ISOLATION, CHARACTERIZATION AND SECONDARY METABOLITE ENDOPHYTIC FUNGAL ISOLATE FROM PERONEMA CANESCENS JACK LEAF AND COPTOSAPELTA TOMENTOSA VAL. K. HEYNE ROOT

Journal Of Tropical Pharmacy And Chemistry ◽

10.25026/jtpc.v4i5.169 ◽

2019 ◽

Vol 4 (5) ◽

pp. 215-225

Author(s):

Arsyik Ibrahim ◽

M. Arifuddin ◽

Wisnu Cahyo P ◽

Wahyu Widayat ◽

Mahfuzun Bone

Keyword(s):

Secondary Metabolites ◽

Thin Layer ◽

Secondary Metabolite ◽

Fungal Isolate ◽

Chromatography Method ◽

Fungal Isolates ◽

Reaction Test ◽

Layer Chromatography ◽

Spray Reagents

Has been done Isolation, Characterization and Secondary Metabolite Endophytic Fungal Isolate from Peronema canescens Jack Leave and Coptosapelta tomentosa Valeton K. Heyne Root. The aim of this research is to know the number of fungal isolates, chromatogram profile and secondary metabolite group of endophytic fungal isolates from P. canencens leaves and C. tomentosa root. Characterization of endophytic fungal isolates was done macroscopically and microscopically. Identification of secondary metabolites endophytic fungal isolates were performed by chemical reaction test and TLC (Thin Layer Chromatography) method with specific spray reagents. The data of this study were obtained based on the number of endophytic fungal that can be isolated, observing macroscopic and microscopic morphological profiles, chromatogram profile and secondary metabolites of each endophytic fungal isolated. The results showed that endophytic fungal that can be isolated from P. canencens leaves four isolates, and two isolates from C. tomentosa root. Morphological profile macroscopic endophytic fungal of the six isolates showed a greenish-colored colony, white gray, clear black. Microscopic profile of each fungal isolate having spores, sprangiosphora, sporangium, conidia, hyphae and stolon. The identified secondary metabolites are: alkaloids, terpenoids, and flavonoids, and polyphenols.

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Genome-wide identification and characterization of the CKII gene family in the cultivated banana cultivar (Musa spp. cv Tianbaojiao) and the wild banana (Musa itinerans)

PLoS ONE ◽

10.1371/journal.pone.0200149 ◽

2018 ◽

Vol 13 (7) ◽

pp. e0200149 ◽

Cited By ~ 2

Author(s):

Weihua Liu ◽

Zhengchun Lin ◽

Yanying Liu ◽

Yuling Lin ◽

XuHan Xu ◽

...

Keyword(s):

Gene Family ◽

Musa Spp ◽

Genome Wide ◽

Wild Banana ◽

Musa Itinerans ◽

Identification And Characterization ◽

Banana Cultivar

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IDENTIFICATION AND CHARACTERIZATION OF Fusarium spp. FROM MUSKMELON IN NORTHWEST MEXICO

Biotecnia ◽

10.18633/biotecnia.v20i3.715 ◽

2018 ◽

Vol 20 (3) ◽

pp. 71-75 ◽

Cited By ~ 2

Author(s):

Tomas Rivas Garcia ◽

Luis Guillermo Hernandez Montiel ◽

Bernardo Murillo Amador ◽

Alejandra Nieto Garibay ◽

Roberto Gregorio Chiquito Contreras ◽

...

Keyword(s):

Cucumis Melo ◽

Fusarium Species ◽

Fusarium Proliferatum ◽

Molecular Techniques ◽

High Susceptibility ◽

Fusarium Spp ◽

Northwest Mexico ◽

Cucumis Melo L ◽

Identification And Characterization

Muskmelon (Cucumis melo L.) is a primary crop of Mexico. Nevertheless, the fruit has a high susceptibility to postharvest fungal diseases. Fusarium species are one of the main causes of diseases that limit production of muskmelon. The objective of this study was to characterize and identify by taxonomic keys and molecular markers species of Fusarium related to rot of muskmelon var. Reticulatus in Northwest Mexico. To identify the causative agent, fruits were collectedm from cultivated fields. The isolated fungi were inoculated on muskmelon to determine its pathogenicity. Morphological analyses as well as molecular techniques confirmed that the pathogen was the fungus Fusarium proliferatum.

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Antibacterial activity of endophytes from Musa Paradisiaca

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11ispl4.4623 ◽

2020 ◽

Vol 11 (SPL4) ◽

pp. 3104-3110

Author(s):

Keerthy Reghunandanan ◽

Aleena Jolly ◽

Manjusha P V ◽

Kavitha M

Keyword(s):

Antibacterial Activity ◽

Blue Staining ◽

Klebsiella Pneumonia ◽

Bacterial Isolates ◽

Bacterial Colony ◽

Musa Paradisiaca ◽

Gram Staining ◽

Fungal Isolates ◽

Bacteria And Fungi ◽

Banana Cultivar

This study was aimed to isolate the endophytic bacteria and fungi from the fruit and root of Musa Paradisiaca, commonly called as Nendran banana cultivar in the southern Indian state of Kerala. Endophytes are microbes living in plant tissue without harming the plants rather existing as potential beneficial organisms. Musa paradisiaca is a less explored variety of banana cultivar which possess anti-disease potential apart from nutritional quality. The total number of twelve root and fruit associated endophytes were isolated. The fruit associated endophytes were seven with four bacterial isolates and three fungal isolates. The number of root-associated endophytes were five with three bacterial isolates and two fungal isolates. The morphological characterisation of both root and fruit endophytes were performed by Gram staining for bacteria and lacto-phenol cotton blue staining for fungi. The bacterial colony examination of the fruit associated culture revealed a specific pink strain with an inhibitory zone proving antibacterial activity which was further investigated through agar well diffusion test. The strain exhibited potential antibacterial activity against five human clinical pathogens. A significant zone of inhibition was observed against Bacillus subtilis (22 mm), Escherichia coli (21 mm), Klebsiella pneumonia and Pseudomonas aeruginosa (18 mm) which confirmed the potential antibacterial property of the fruit endophytic strain.

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