scholarly journals Effect Of Different Sugars On Shoot Regeneration Of Maize (Zea Mays L.)

1970 ◽  
Vol 8 (1) ◽  
pp. 119-124 ◽  
Author(s):  
DP Gauchan
Keyword(s):  
Zea Mays ◽  
Root Growth ◽  
Shoot Regeneration ◽  
Growth Response ◽  
Carbon Sources ◽  
Seedling Development ◽  
Good Growth ◽  
Low Concentration ◽  
Basal Media

Effect of various concentrations of different sugars was investigated for induction of root and shoot from maize. The seedling development was induced with the application of fructose, maltose and sucrose at different concentrations (0.25%, 0.5%, 0.75%, 1.0%, 2.5%, 5%, and 10%) of each. Dissected embryos were transferred in ½ MS basal media fortified with various concentrations of different carbon sources. In vitro regenerated maize plantlets were healthy and attained a length of 12.2 cm at 1.0 % concentration in maltose within a week. Out of three sugars low concentration (0.25%- 2.5%) of maltose and sucrose exhibited the maximum shoot and root growth. All the concentrations of maltose and sucrose showed the good growth response of shoot and root. DOI: http://dx.doi.org/10.3126/kuset.v8i1.6051 KUSET 2012; 8(1): 119-124

scholarly journals In vitro propagation of Manihot esculenta Crantz in alternative substrate: Ammonium nitrate, potassium bi phosphate, Zea mays extracts and soil

2020 ◽  
Vol 8 (10) ◽  
pp. 237-244
Author(s):  
Duru Maduabuchi ◽  
◽  
Mbata Ikechukwu ◽  
Osikwe Keziah ◽  
Ukaoma Adamma ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Zea Mays ◽  
Somatic Cell ◽  
Ammonium Nitrate ◽  
Shoot Regeneration ◽  
Manihot Esculenta ◽  
In Vitro Propagation ◽  
Gelling Agent ◽  
Manihot Esculenta Crantz

The study investigated an in vitro propagation of Manihot esculenta Crantz in a substituted substrate regime. The aim was to proffer and affordable alternative to the expensive high tech media formulations usually employed in tissue culture protocol. The experiment was conducted on laboratory bench, using standard tissue culture and micropropagation methods under aseptic conditions. The morphogenesis effect of the substrate was determined based on the integer number of explants’ callus and adventitious shoot regeneration. Results showed that MS + Agar, supported embryogenic callus formation with 38% viability, NH4NO3 + KH2PO4 + Agar, supported same with 29%. MS + 2, 4-D + BAP +Agar supported shoot establishment with 32%. While NH4NO3 + KH2PO4 + Zea mays extracts + Agar, did same with 43.26%. MS + Soil, supported callugenesis with 27% viability while NH4NO3 + KH2PO4 + Soil supported the callus establishment with 25%. MS + 2,4 - D + BAP + Soil, supported shoot establishment with 38.41% viability while NH4NO3 + KH2PO4 + Zea mays Extracts + Soil supported same with 36%. The application of crude Zea mays seedling extracts can serve as potent alternative to the synthetic 2, 4 – D and BAP, in in vitro somatic cell morphogenesis. NH4NO3 + KH2 + PO4 can substitute for the MS salt in the same protocol. Loamy top soil can be a good alternative to agar powder as gelling agent in cassava somatic cell embryogenesis and shoot regeneration. Keywords: Ammonium nitrate, Potassium biphosphate, MS salt, axillary meristem, morphogenesis.

Influence of some carbon sources on growth of Cochliobolus sativus

1972 ◽  
Vol 50 (3) ◽  
pp. 683-685
Author(s):  
R. V. Clark
Keyword(s):  
Radial Growth ◽  
Mycelial Growth ◽  
Growth Response ◽  
Carbon Sources ◽  
Cochliobolus Sativus ◽  
Good Growth ◽  
Liquid Media ◽  
Differential Growth ◽  
Agar Media

Four isolates of C. sativus were able to use a number of carbon sources to varying degrees with little evidence of a differential growth response by the isolates. With most carbon sources the response was different when growth on liquid media was compared with that on agar media. Dextrin and L-sorbose were exceptions as dextrin supported good growth with both types and L-sorbose poor with both. Lactose supported the best mycelial growth on liquid media and dextrin the best radial growth and sporulation on agar media when compared with sucrose.

scholarly journals Cultivation of micro-algae for Production of Biodiesel: An optimized Process

2016 ◽  
Vol 7 ◽  
Author(s):  
Abebe Girma Demissie ◽  
Bhaskarrao Chinthapalli ◽  
Shumet Tenaw ◽  
D. S. Vijaya Chitra
Keyword(s):  
Algal Biomass ◽  
Incubation Temperature ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Biodiesel Production ◽  
Potential Candidate ◽  
Potential Source ◽  
Microalgae Biomass ◽  
Basal Media

<p>Microalgae are considered as one of the potential source of biodiesel for the future. The search to obtain the potential strains from the algal diversity capable of producing oil is critical for sustainable production of biodiesel. In the present study, microalgae biomass with oil/lipid accumulation capability and their morphological features was isolated from Lake Abaya and Chamo. The algal biomass was cultivated <em>in vitro</em> and media optimization for maximum biomass was done using different basal media, BG-11 medium, and Chu -10. In addition the various carbon sources, nitrogen sources, pH and temperature were considered in this study for optimization. Green algae <em>Oedogonium</em>, <em>Chlorella </em>and <em>Cladophora</em> species were observed to be dominant species and the maximum oil per dry algal biomass was found to be from <em>Oedogonium</em> sp. Thus from the present study for the cultivation of the selected algae, BG-11 medium supplemented with tryptone (0.2%) sucrose (2%) and pH- 6 with incubation temperature of 30<sup>0</sup>C was found to be suitable.  These results suggest that <em>Oedogonium</em> sp. has several desirable features that make it a potential candidate for biodiesel production.</p>

In vitro and transplantation experiments with germination of date embryos

1992 ◽  
Vol 70 (5) ◽  
pp. 965-974
Author(s):  
Darleen A. DeMason ◽  
Daniel Widney ◽  
James I. Stillman
Keyword(s):  
Carbon Source ◽  
Date Palm ◽  
Carbon Sources ◽  
Culture Conditions ◽  
Good Growth ◽  
Anatomical Features ◽  
Developmental Characteristics ◽  
Transplantation Experiments ◽  
Better Than

In vitro experiments with date palm embryos were designed to determine (i) which carbon sources support growth and (ii) what culture conditions promote haustorial growth and development. Date palm embryos were also transplanted into Washington filifera seeds to deterine whether any developmental characteristics were determined by the seed or endosperm. Date embryos did not grow on media containing starch or galactomannan any better than they do on medium lacking a carbon source. Good growth occurred on medium containing mannose, glucose, or sucrose as the carbon source. Embryos imbibed in the seed for 6 days grew better and produced larger haustoria than those imbibed 2 days, imbibed in water, imbibed in 3% mannose, or unimbibed. Date embryos imbibed in Washington seeds grew as well as those imbibed in date seeds. Optimum seedling and haustorium weights occurred at pH 7. The transembryonic seedlings germinated and grew normally but the shape of the haustorium was altered. Haustoria produced by in vitro grown seedlings exhibited many normal anatomical features, but haustoria from transembryonic seedlings exhibited more normal histological features. Results support the hypotheses that (i) date embryos do not possess the enzymes necessary to digest mannans and (ii) haustorium development is controlled by at least two steps, an initial inductive step that occurs by 6 days of imbibition and a further step that occurs later. We hypothesize that diffusable substances from the endosperm are responsible for controlling haustorium initiation and development. Key words: germination, embryo culture, palms, palm tissue culture, Arecaceae, haustorium.

scholarly journals In vitro Shoot Regeneration and Control of Shoot Tip Necrosis in Tissue Cultures of Soymida febrifuga (Roxb.) A. Juss.

2012 ◽  
Vol 21 (1) ◽  
pp. 11-25 ◽  
Author(s):  
Kishore K. Chiruvella ◽  
Arifullah Mohammed ◽  
Gayathri Dampuri ◽  
Rama Gopal Ghanta
Keyword(s):  
Shoot Regeneration ◽  
Carbon Sources ◽  
Multiple Shoots ◽  
Culture Conditions ◽  
Shoot Tip ◽  
Nodal Explants ◽  
Nodal Segments ◽  
Deciduous Tree ◽  
Shoot Tip Necrosis

Soymida febrifuga (Roxb.) A. Juss., (Meliaceae) an indigenous lofty deciduous tree, endemic to India, has extensive pharmacological activity. Continuous destruction of plants due to environmental and geopolitical instability has posed a major threat to endemic tree species. In vitro propagation techniques are also problematic due to high incidence of shoot tip necrosis (STN). The effect of various culture conditions on regeneration and STN of Soymida febrifuga were investigated. Nodal segments from field grown plants were used as explants and cultured on MS supplemented with different concentrations of BA, KN, NAA and IAA. The synergistic effect of BA (2 mg/l) and NAA (0.2 mg/l) induced a mean of 6.3 ± 0.09 shoots from the nodal explants with a frequency of 80.4%. Frequent subculturing of nodal explants from in vitro derived shoots increases the number of multiple shoots, but the regenerated shoots exhibited the symptoms of STN. Various factors such as strength of the media, different carbon sources activated charcoal and different calcium sources have been investigated for reducing the incidence of shoot necrosis. STN can easily be recovered by increasing the levels of calcium. Transfer of shoots showing early signs of necrosis to half strength MS supplemented with CN (556 mg/l), CP (1.0 mg/l), AC (20 mg/l) and fructose (100 mg/l) facilitated recovery of more than 98% of the shoots. Rooted plantlets produced, using the optimized protocol, were acclimatized successfully.   Key words: Soymida febrifuga, Shoot regeneration, Shoot tip necrosis   D.O.I. 10.3329/ptcb.v21i1.9559   Plant Tissue Cult. & Biotech. 21(1): 11-25, 2011 (June)

INFLUENCE OF TEMPERATURE ON THE VIABILITY, VIRULENCE, AND PHYSIOLOGIC CHARACTERISTICS OF XANTHOMONAS PHASEOLI VAR. FUSCANS IN VIVO AND IN VITRO

1966 ◽  
Vol 44 (10) ◽  
pp. 1239-1245 ◽  
Author(s):  
P. K. Basu ◽  
V. R. Wallen
Keyword(s):  
Carbon Sources ◽  
Good Growth ◽  
Sugar Alcohols ◽  
Pigment Formation ◽  
Influence Of Temperature ◽  
Physiological Properties ◽  
Influence Of Temperature On ◽  
Physiological Characters

Xanthomonas phaseoli var. fuscans (Burkh.) Starr & Burkh. in naturally infected bean seeds stored at −20 to 35 °C retained its viability, virulence, and physiological properties for 3 years. Agar cultures of the pathogen maintained at −20 to 20 °C also retained their pathogenicity and physiological characters throughout the test period but showed less virulence than the seed-borne isolates by the end of 33 months. The viability of agar cultures held at 25, 30, and 35 °C was lost after 24, 18, and 9 months of storage, respectively.The effects of 24 carbon sources on the growth of the pathogen have been determined. The production of a brown diffusible pigment by the organism was influenced by temperature, aeration, and the carbon source. Sugar alcohols did not support good growth or inhibit pigment formation. Most carbohydrates, which supported good or fair growth of the organism, seemed to inhibit pigment production or its expression.

scholarly journals Effect of Different Carbon Sources on in Vitro Shoot Regeneration of Petunia Hybrid Hort. Vilm

2018 ◽  
Vol Volume-2 (Issue-4) ◽  
pp. 2540-2542
Author(s):  
Assia Zahoor ◽  
Jasfeeda Qadir ◽  
Dr. Seema Singh ◽  
Keyword(s):  
Shoot Regeneration ◽  
Carbon Sources ◽  
In Vitro Shoot Regeneration

scholarly journals Effect of Basal Media and Sugar Types on in Vitro Regeneration of Grammatophyllum speciosum Blume

2011 ◽  
Vol 3 (3) ◽  
pp. 101-104 ◽  
Author(s):  
Montakarn PIMSEN ◽  
Kamnoon KANCHANAPOOM
Keyword(s):  
Solid Medium ◽  
In Vitro Regeneration ◽  
Carbon Sources ◽  
Inhibitory Effect ◽  
Ms Medium ◽  
Sugar Alcohols ◽  
Immature Seeds ◽  
Basal Media ◽  
Positive Effect

Protocorms of Grammatophyllum speciosum Blume. were initiated from immature seeds on solid MS medium containing 15% (v/v) coconut water (CW) and 3% (w/v) sucrose. Protocorms, 2-4 mm in length were used as explants and subcultured to MS and VW (Vacin and Went, 1949) media containing 15% (v/v) CW and 2 or 3% (w/v) sucrose. Protocorms gave the highest formation of PLBs (protocorm-like bodies) at 3.1 PLBs/explant on MS solid medium containing 15% CW and 2% sucrose. For the test with different carbon sources, protocorms were cultured in liquid MS medium supplemented with 4 kinds of sugar, namely sucrose, glucose, sorbitol and mannitol at 2, 4, 6 or 8% (w/v) and cultured for 1, 2, 3 or 4 weeks. They were then transferred to MS solid medium containing 15% CW and 2% sucrose. Results revealed that after 4 weeks in MS liquid medium, sucrose and glucose had an inhibitory effect and 8% glucose gave a high percentage of protocorm browning. In contrast, sorbitol and mannitol were effective for protocorm regeneration and both sugar alcohols had a positive effect on the formation of PLBs and the development of PLBs into plantlets.

scholarly journals Growth regulators, culture media and antibiotics in the in vitro shoot regeneration from mature tissue of citrus cultivars

2010 ◽  
Vol 45 (7) ◽  
pp. 654-660 ◽  
Author(s):  
Maria Luiza Peixoto de Oliveira ◽  
Marcio Gilberto Cardoso Costa ◽  
Crislene Viana da Silva ◽  
Wagner Campos Otoni
Keyword(s):  
Shoot Regeneration ◽  
Growth Regulators ◽  
Culture Media ◽  
Naphthaleneacetic Acid ◽  
Beta Lactam ◽  
Rangpur Lime ◽  
Beta Lactam Antibiotics ◽  
Bud Induction ◽  
Basal Media

The objective of this study was to evaluate the effects of 6-benzylaminopurine (BAP) and α-naphthaleneacetic acid (NAA) combinations, basal media and beta-lactam antibiotics on in vitro organogenesis from mature stem segments of 'Pêra', 'Valência' and 'Bahia' sweet oranges and 'Cravo' rangpur lime. For induction of shoot regeneration, the segments of the four cultivars were placed on Murashige and Skoog (MS) medium containing the following BAP/NAA concentrations: 0.0/0.0; 0.25/0.0; 0.25/0.25; 0.5/0.0; 0.5/0.5; 1.0/0.0; 2.0/0.0; 2.0/0.25; 2.0/0.5; and 2.0/1.0 mg L-1. In order to test the influence of the culture media on shoot-bud induction, (MS), Murashige and Tucker (MT), and woody plant medium (WPM) formulations were evaluated, associated with the best combination of plant growth regulators obtained in the previous experiment. The influence of four beta-lactam antibiotics (timentin, cefotaxime sodium salt, meropenem trihydrate and augmentin) on shoot regeneration was determined. Better regeneration responses were achieved when internodal segments were cultured onto MS-based medium with 500 mg L-1 cefotaxime with the following BAP/NAA concentrations: 0.5 + 0.25 mg L-1 for 'Cravo', 1.0 + 0.25 mg L-1 for 'Valência' and 'Bahia', and 1.0 + 0.5 mg L-1 for 'Pêra'. Genotype, growth regulators, basal media and beta-lactam antibiotics affect the morphogenetic response in mature tissues of citrus.

scholarly journals IN VITRO SHOOT REGENERATION FROM WESTERN SOAPBERRY

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 697c-697
Author(s):  
Mahmoud B. Arif ◽  
Houchang Khatamian
Keyword(s):  
Nicotinic Acid ◽  
Shoot Regeneration ◽  
Culture Media ◽  
Callus Formation ◽  
Ms Medium ◽  
Full Strength ◽  
Basal Media ◽  
In Vitro Shoot Regeneration

Surface sterilized stem nodal sections of western soapberry (Sapindus drummondii Hook. & Arn.) were cultured on Murashige and Skoog (MS) medium. The basal media consisted of one half and full strength MS medium each supplemented with the following (mg-1): Nicotinic acid 0.5, pyridoxine Hcl 0.5, Glycine 2.0, myo-inositol 100, sucrose 30,000 and agar 8000. Each medium also was supplemented with either 0, 0.01, 0.1, 1.0 and 10 mg/l Thidiazuron (TZD) or 0, 0.5, 2.0 and 5 mg/l 6-Benzyladenine (BA). The pH of all media was adjusted to 5.8 ± 0.1. The culture media were autoclaved at 120°C at 1.5 Kgcm-1 pressure for 15 min. The highest percentage of nodal sections resulting in shoot regeneration occurred on 1/2 MS with TZD at 0.01 mg/l and MS medium containing 0.5 mg/l of BA Increasing the TZD concentration above 0.1 mg/l resulted in callus formation on cut surfaces.

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