Immunohistochemistry of Lymphocytes Subsets in Subclinical Experimental Paratuberculosis in Goats

2019 ◽  
Vol 36 ◽  
pp. 1-7
Author(s):  
U. M. Singh ◽  
B. N. Tripathi ◽  
O. P. Paliwal
Keyword(s):  
Lymph Node ◽  
Mycobacterium Avium ◽  
Present Experiment ◽  
Cellular Reaction ◽  
Mhc I ◽  
Mycobacterium Avium Paratuberculosis ◽  
Infected Control ◽  
Lymphocytes Subsets ◽  
Experimentally Induced

 The present experiment was carried out to find out the lymphocytes subsets reactions in experimentally induced subclinical paratuberculosis in goats. Twelve goats of 8-12 weeks age were infected with 4.23 x 109 Mycobacterium avium paratuberculosis on 8 occasions. Seven goats were kept as in-contact controls and 4 as uninfected controls. Immunohitochemistry for detection of cellular reaction of CD2+, CD4+, CD8+, CD25+, MHC I and MHCII in the lymphocytes present in the intestine and lymph node revealed more reactive cells in the infected goats as compared with the in-contact and infected control goats.

scholarly journals Antibodies Recognizing Mycobacterium avium paratuberculosis Epitopes Cross-React with the Beta-Cell Antigen ZnT8 in Sardinian Type 1 Diabetic Patients

PLoS ONE ◽  
2011 ◽  
Vol 6 (10) ◽  
pp. e26931 ◽  
Author(s):  
Speranza Masala ◽  
Daniela Paccagnini ◽  
Davide Cossu ◽  
Vedran Brezar ◽  
Adolfo Pacifico ◽  
...  
Keyword(s):  
Beta Cell ◽  
Mycobacterium Avium ◽  
Diabetic Patients ◽  
Mycobacterium Avium Paratuberculosis ◽  
Cell Antigen ◽  
Type 1 Diabetic Patients

scholarly journals Mycobacterium avium paratuberculosis infection of calves – The impact of dam infection status

2020 ◽  
Vol 181 ◽  
pp. 104634 ◽  
Author(s):  
S. Patterson ◽  
K. Bond ◽  
M. Green ◽  
S. van Winden ◽  
J. Guitian
Keyword(s):  
Mycobacterium Avium ◽  
Infection Status ◽  
Mycobacterium Avium Paratuberculosis ◽  
The Impact

scholarly journals Notch-1 Signaling Modulates Macrophage Polarization and Immune Defense against Mycobacterium avium paratuberculosis Infection in Inflammatory Diseases

2020 ◽  
Vol 8 (7) ◽  
pp. 1006 ◽  
Author(s):  
Esra’a Keewan ◽  
Saleh A. Naser
Keyword(s):  
Inflammatory Response ◽  
Notch Signaling ◽  
Mycobacterium Avium ◽  
Macrophage Polarization ◽  
Immune Defense ◽  
Notch 1 ◽  
Macrophage Response ◽  
Mycobacterium Avium Paratuberculosis ◽  
Vitro Effect

Despite the extensive research on Notch signaling involvement in inflammation, its specific role in macrophage response in autoimmune disease and defense mechanisms against bacterial infection, such as Mycobacterium avium paratuberculosis (MAP), remains unknown. In this study, we investigated the molecular role of Notch-1 signaling in the macrophage response during MAP infection. In particular, we measured the in vitro effect of MAP on Notch-1 signaling and downstream influence on interleukin (IL)-6 and myeloid cell leukemia sequence-1 (MCL-1) and consequent cellular apoptosis, MAP viability, and macrophage polarization. Overall, the data show significant upregulation in Notch-1, IL-6, and MCL-1 in MAP-infected macrophages, parallel with a decrease in apoptosis and elevated pro-inflammatory response in these infected cells. On the contrary, blocking Notch signaling with γ-secretase inhibitor (DAPT) decreased MAP survival and burden, increased apoptosis, and diminished the pro-inflammatory response. In particular, the treatment of infected macrophages with DAPT shifted macrophage polarization toward M2 anti-inflammatory phenotypic response. The outcome of this study clearly demonstrates the critical role of Notch signaling in macrophage response during infection. We conclude that MAP infection in macrophages activates Notch-1 signaling and downstream influence on IL-6 which hijack MCL-1 dependent inhibition of apoptosis leading to its chronic persistence, and further inflammation. This study supports Notch-1 signaling as a therapeutic target to combat infection in autoimmune diseases such as Crohn’s disease and Rheumatoid Arthritis.

scholarly journals Gastrointestinal: Mycobacterium avium paratuberculosis and Crohn's disease

2005 ◽  
Vol 20 (12) ◽  
pp. 1943-1943 ◽  
Author(s):  
RB Gearry ◽  
JM Aitken ◽  
RL Roberts ◽  
S Ismail ◽  
J Keenan ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Mycobacterium Avium ◽  
Mycobacterium Avium Paratuberculosis

scholarly journals Host Responses to Persistent Mycobacterium avium SubspeciesparatuberculosisInfection in Surgically Isolated Bovine Ileal Segments

2012 ◽  
Vol 20 (2) ◽  
pp. 156-165 ◽  
Author(s):  
Chandrashekhar Charavaryamath ◽  
Patricia Gonzalez-Cano ◽  
Patrick Fries ◽  
Susantha Gomis ◽  
Kimberley Doig ◽  
...  
Keyword(s):  
Lymph Node ◽  
T Cell ◽  
Lamina Propria ◽  
Mycobacterium Avium ◽  
Mesenteric Lymph Node ◽  
Cell Receptor ◽  
Host Responses ◽  
Necrosis Factor Alpha ◽  
Content Type ◽  
Mesenteric Lymph

ABSTRACTA lack of appropriate disease models has limited our understanding of the pathogenesis of persistent enteric infections withMycobacterium aviumsubsp.paratuberculosis. A model was developed for the controlled delivery of a defined dose ofM. aviumsubsp.paratuberculosisto surgically isolated ileal segments in newborn calves. The stable intestinal segments enabled the characterization of host responses to persistentM. aviumsubsp.paratuberculosisinfections after a 9-month period, including an analysis of local mucosal immune responses relative to an adjacent uninfected intestinal compartment.M. aviumsubsp.paratuberculosisremained localized at the initial site of intestinal infection and was not detected by PCR in the mesenteric lymph node.M. aviumsubsp.paratuberculosis-specific T cell proliferative responses included both CD4 and γδ T cell receptor (γδTcR) T cell responses in the draining mesenteric lymph node. The levels of CD8+and γδTcR+T cells increased significantly (P< 0.05) in the lamina propria, andM. aviumsubsp.paratuberculosis-specific tumor necrosis factor alpha (TNF-α) and gamma interferon secretion by lamina propria leukocytes was also significantly (P< 0.05) increased. There was a significant (P< 0.05) accumulation of macrophages and dendritic cells (DCs) in the lamina propria, but the expression of mucosal toll-like receptors 1 through 10 was not significantly changed byM. aviumsubsp.paratuberculosisinfection. In conclusion, surgically isolated ileal segments provided a model system for the establishment of a persistent and localized entericM. aviumsubsp.paratuberculosisinfection in cattle and facilitated the analysis ofM. aviumsubsp.paratuberculosis-specific changes in mucosal leukocyte phenotype and function. The accumulation of DC subpopulations in the lamina propria suggests that further investigation of mucosal DCs may provide insight into host responses toM. aviumsubsp.paratuberculosisinfection and improve vaccine strategies to preventM. aviumsubsp.paratuberculosisinfection.

Serological, culture and molecular survey of Mycobacterium avium paratuberculosis in a goat flock in Tuscany

2017 ◽  
Vol 62 (6) ◽  
pp. 471-477 ◽  
Author(s):  
Alessia Galiero ◽  
Barbara Turchi ◽  
Francesca Pedonese ◽  
Roberta Nuvoloni ◽  
Carlo Cantile ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Mycobacterium Avium Paratuberculosis ◽  
Molecular Survey ◽  
Goat Flock
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