Enrichment of surface water with nitrate-nitrogen (NO3-N) is a significant problem throughout the world. In support of developing a method for removing NO3-N from water using denitrification, this project characterized runoff events at two nurseries in southern Florida to provide information needed for designing capacity. Specifically, estimates of runoff rates and volumes, NO3-N concentrations and loadings were profiled during intensively sampled fertigation events at a foliage plant nursery and a bedding plant nursery. Discharge volumes and rates varied with event, ranging from 2,487 to 20,935 L and 59.2 to 126 L·min−1, respectively, per event. NO3-N concentrations ranged from 0.7 to 386.4 mg·L−1, and event loadings ranged from 51 to 3024 g, indicating that significant losses may be realized. This project provided valuable hydraulic and chemical loading information needed for the development and design of bioremediation tools for the horticultural industry.