Protective effect of 24-epibrassinolide on wheat plants under water deficit

We studied the effect of 24-epibrassinolide (EB) on growth, proline content, the state of wheat seedling antioxidant system under water deficit, modeled by 12% polyethylene glycol (PEG). It was found that under drought EB-pretreatment has a protective effect on wheat plants, stabilizing the state of the antioxidant system, regulating the balance of hydrogen peroxide and peroxidase activity, as well as inducing the additional accumulation of proline, which was reflected in maintaining a higher level of mitotic activity in apical root cells of these plants.

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The influence of selective light on the growth reaction and antioxidant system of seedlings Pisum sativum L.

Faktori eksperimental'noi evolucii organizmiv ◽

10.7124/feeo.v29.1428 ◽

2021 ◽

Vol 29 ◽

pp. 179-184

Author(s):

O. O. Avksentieva ◽

E.D. Batueva

Keyword(s):

Hydrogen Peroxide ◽

Pisum Sativum ◽

Antioxidant System ◽

Growth Response ◽

The State ◽

Aboveground Part ◽

Pisum Sativum L ◽

Etiolated Seedlings ◽

Growth Reaction ◽

Axial Organs

Aim. Study of the effect of red (660 nm), green (530 nm) and blue (450 nm) light on the growth processes and the state of the antioxidant system in the axial organs of seedlings of pea plants. Methods. Etiolated seedlings of pea Maecenat variety were irradiated with selective light with different spectrum of RL (660 nm), GL (530 nm), BL (450 nm) to activate photoreceptor systems of plants. In 10-day-old seedlings, growth response was determined – linear growth and biomass accumulation, as well as indicators of antioxidant system – hydrogen peroxide content and activity of oxidases – catalase and nonspecific peroxidase in axial organs of seedlings: in the aboveground part and roots. Results. Irradiation of the RL and the GL stimulates the accumulation of seedling biomass in the aboveground part and roots. BL inhibits the growth response of seedlings. The maximum stimulating effect is shown by the GL. The state of the antioxidant system in etiolated seedlings is characterized by organ specificity. Under the action of selective light, the content of the main form of ROS – hydrogen peroxide and shoots and in the roots, significantly stimulates the activity of catalase and peroxidase enzymes in the aboveground part of the seedling and is inhibited in the roots. The maximum effect in the aboveground part is shown by the GL, in the roots of the RL and the BL. Conclusions. The established effects of selective light irradiation are manifested differently in the aboveground and underground parts of seedlings. Possible mechanisms of connection of a condition of antioxidant system with separate aspects of signaling in photomorphogenesis of plants are discussed. Keywords: Pisum sativum L., selective light, RL (660 nm), GL (530 nm), BL (450 nm), growth reaction, axial organs, H2O2, catalase, peroxidase.

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Correction by "Quertin" of the oxidative-antioxidant system of rats at xenobiotics exposure

V N Karazin National University Series “Biology” ◽

10.26565/2075-5457-2018-31-1 ◽

2018 ◽

Keyword(s):

Superoxide Dismutase ◽

Polyethylene Glycol ◽

Ethylene Glycol ◽

Antioxidant System ◽

Catalase Activity ◽

The State ◽

Intragastric Administration ◽

Polypropylene Glycol ◽

Polyethylene Glycol 400 ◽

Flavonoid Quercetin

The objective of this study is to determine the possibility of correcting pathological disorders of the oxidative-antioxidant system in the rat organism under the influence of xenobiotics using the flavonoid quercetin, which has an antioxidant, anti-inflammatory, antibacterial, antiviral and immunomodulating effect. Baseline studies have established that when exposed to xenobiotics at a dose of 1/10 and 1/100 DL50, the content of lipid peroxidation products in the serum of rats increases, including 8-isoprostane, TBA-active products (TBA-AP) and diene conjugates (DK). As a result, the state of the antioxidant system also undergoes changes, evidenced by a decrease in catalase activity under the action of xenobiotics in doses of 1/10 and 1/100 DL50, as well as fluctuations in superoxide dismutase content, namely: a decrease under the influence of xenobiotics in a dose of 1/10 DL50 and increase with the action of substances in a dose of 1/100 DL50. After correction with the flavonoid quercetin, a decrease in the content of both primary and secondary POL products in the rat organism, as well as indicators of the state of the oxidative-antioxidant system was established. At the same time, an important for clinical practice relationship was established between the degree of correction of pathological changes in the state of the oxidative-antioxidant system and the dose of toxic effects of xenobiotics. After intragastric administration of “Quertin” in a dose of 25 mg/kg of body weight to rats exposed to polyethylene glycol 400 at a dose of 1/10 DL50, a decrease in serum levels of 8-isoprostan was determined by 14.5%, TBA-AP – by 17.3%, DK – by 15.5%. After exposure to polyethylene glycol 400 at a dose of 1/100 DL50, the content of 8-isoprostane decreased by 12.4%, TBА-AP by 16.8%, and DK by 11.8%. After exposure to polypropylene glycol in doses of 1/10 and 1/100 DL50, the content of 8-isoprostane decreased by 17.7% and 12.5%, TBA-AP – 11.7% and 9.8%, DK – 16.3% and 12.7% respectively. After exposure to ethylene glycol in doses of 1/10 and 1/100 DL50, the content of 8-isoprostane decreased by 22.1% and 14.9%, TBA-AP – 17.3% and 15.2%, DK – 17.6% and 12.2% respectively. Catalase activity increased after the correction by “Quertin” at exposure to polyethylene glycol 400 at doses 1/10 and 1/100 DL50, respectively, by 25.8% and 20.6%; polypropylene glycol – by 26.5% and 23.4%; ethylene glycol – by 19.4% and 15.6%. Superoxide dismutase activity in the blood of rats after the correction of “Quertin” increased at xenobiotic toxification at a dose of 1/10 DL50 (polyethylene glycol 400 – by 29.3%, polypropylene glycol – by 33.5%; ethylene glycol – by 23.2%) and decreased at toxification with xenobiotics at a dose of 1/100 DL50 (polyethylene glycol 400 – by 21.6%, polypropylene glycol – by 26.7%; ethylene glycol – by 18.6%).

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Hydrogen peroxide modulates antioxidant system and nutrient relation in maize (Zea maysL.) under water-deficit conditions

Archives of Agronomy and Soil Science ◽

10.1080/03650340.2014.938644 ◽

2014 ◽

Vol 61 (4) ◽

pp. 507-523 ◽

Cited By ~ 31

Author(s):

Muhammad Arslan Ashraf ◽

Rizwan Rasheed ◽

Iqbal Hussain ◽

Muhammad Iqbal ◽

Muhammad Zulqurnain Haider ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Water Deficit ◽

Antioxidant System

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Hydrogen peroxide acts as a signal molecule in CO2 laser pretreatment-induced osmotic tolerance in wheat seedling

Plant Soil and Environment ◽

10.17221/271/2010-pse ◽

2011 ◽

Vol 57 (No. 9) ◽

pp. 403-408 ◽

Cited By ~ 3

Author(s):

Z.B. Qiu ◽

Q. Li ◽

Z.Z. Bi ◽

M. Yue

Keyword(s):

Hydrogen Peroxide ◽

Osmotic Stress ◽

Protective Effect ◽

Stress Responses ◽

Wheat Seedling ◽

Physiological Effect ◽

Signal Molecule ◽

Wheat Seedlings ◽

Water Tolerance ◽

Promotive Effect

The objective of this study was to test whether hydrogen peroxide (H2O2) is involved in laser pretreatment-induced water tolerance in wheat seedlings due to its nature as a second messenger in stress responses. The results showed that 3 min laser pretreatment could enhance water tolerance in wheat seedlings by decreasing the concentration of malondialdehyde (MDA), the production rate of superoxide radical (O2–), and increasing the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) and the concentration of glutathione (GSH), and having a positive physiological effect on the growth of osmotic stress seedlings. But the promotive effect of laser pretreatment-induced water tolerance in wheat seedling was effectively reversed by addition of 2 mmol AsA (ascorbic acid) or 5 µmol DPI (diphenyle iodonium), but exogenous 100 U/mL CAT could not reversed laser pretreatment-induced protective effect on wheat seedlings under osmotic stress. The results suggest that H2O2 metabolism was involved as signal in the processes of laser-induced water acclimation and laser-induced protective effect was shown to be likely related to NADPH oxidase-dependent H2O2 production.

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Stress-protective effect of putrescine and spermine on wheat plants during soil drought

Vìsnik Harkìvsʹkogo nacìonalʹnogo agrarnogo unìversitetu. Serìâ Bìologiâ ◽

10.35550/vbio2020.03.058 ◽

2020 ◽

Vol 2020 (3) ◽

pp. 58-70

Author(s):

O. I. Kokorev ◽

◽

M. A. Shkliarevskyi ◽

Keyword(s):

Protective Effect ◽

Water Deficit ◽

Triticum Aestivum L ◽

Proline Content ◽

Soil Drought ◽

Anthocyanin Content ◽

Protective Effects ◽

Water Metabolism ◽

Soil Culture ◽

Drought Conditions

Polyamines are considered multifunctional stress metabolites in plants. The ability of exogenous polyamines to increase plant resistance to adverse factors of various nature is well-known. At the same time, the stress-protective effects of polyamines at the level of whole plants under conditions of dehydration close to natural have not been sufficiently studied. The aim of this work was to study the effect of foliar treatment of wheat plants with solutions of putrescine and spermine on the functioning of their protective systems during drought under laboratory soil culture conditions. In the experiments, we used young wheat plants (Triticum aestivum L.), which were subjected to a 4-day drought with a gradual decrease in the water content in the soil to 25% of the total moisture capacity. Spraying plants with putrescine in a concentration range of 0,25-5 mM significantly reduced the growth-inhibiting effect of drought; the effect of spermine was less effective, but also significant at P ≤ 0,05. Putrescine significantly reduced the manifestation of water deficit caused by drought. Under the action of spermine, only a tendency towards a decrease in the water deficit of the leaves was noted. Drought caused the effect of oxidative stress, which was manifested in an increase in the content of malondialdehyde (MDA) in leaves. During the pretreatment of plants with spermine, the increase in the MDA content was partially leveled, and under the action of putrescine it was leveled almost completely. Treatment of plants with both polyamines at concentrations of 1 and 5 mM promoted preservation of the pool of chlorophylls and carotenoids in leaves under stress conditions. Moreover, when plants were treated with putrescine and spermine under drought conditions, a close to usual ratio of chlorophylls a/b was maintained. The proline content in leaves increased significantly under the influence of drought. Pretreatment with 1 and 5 mM putrescine, and 5 mM spermine reduced effect of proline content growth in leaves, caused by drought. At the same time, the treatment of plants with both polyamines caused the accumulation of sugars in the leaves. Under the influence of drought, the content of anthocyanins and flavonoids absorbing in the UV-B region significantly decreased in the leaves. Pretreatment with spermine somewhat mitigated the negative effect of drought on the anthocyanin content. Under the action of both putrescine and spermine, the content of flavonoids absorbing in UV-B stabilized in leaves. It was concluded that the protective effect of polyamines on wheat plants under drought conditions is primarily due to the regulation of water metabolism and the prevention of oxidative damage.

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