scholarly journals Physico-Chemical and Pathogenic Contamination Status of Groundwater in the Kilinochchi District, Sri Lanka

Author(s):  
M.G.Y.L. Mahagamage ◽  
I. Abinaiyan ◽  
P.M. Manage
2021 ◽  
Vol 6 (4) ◽  
pp. 217-221
Author(s):  
JM Dahanayake ◽  
◽  
PK Perera ◽  
P Galappatty ◽  
P Fernando ◽  
...  

Tinospora cordifolia (wild) Hook.f. (Thomas) is an important medicinal plant distributed throughout in Sri Lanka and commonly known as Rasakinda in Sinhala and Giloy in English. It is widely used in Sri Lankan traditional medical system and Ayurveda for the treatment of diabetes mellitus, fever, arthritis, skin diseases and for Rasayana (rejuvenating) therapies due to its anti-inflammatory, hypoglyceamic, immunomodulatory, antioxident, anti-allergy, antipyretic, antiarthritic and various other medicinal properties. Imported T. cordifolia stems and Sri Lankan grown T. cordifolia stems can be found in Sri Lankan herbal market. Moreover, different varieties of Tinospora species known as Tikthakinda, Bukinda and Gatakinda are available in the market under the name of Rasakinda which leads to adulteration. Hence, in this research an attempt was made to develop standards for genuine T. cordifolia grown in Sri Lanka. Stems of T. cordifolia evaluated for macroscopical, microscopical, physico-chemical, phytochemical constituents, TLC and HPTLC fingerprint patterns. Microscopical examinations of T. cordifolia stem exhibited the wheel shaped appearance at the transverse cut surface, which is a main characteristic feature of the family Menespermaceae. Stem also showed the mucilage cells and abundant starch granules. Percentages of total ash, water soluble ash and acid insoluble ash, were 9.1%+0.1, 2.31%+0.1, <0.1% respectively. Among the percentages of extractable matter of T. cordifolia, highest amount was shown in hot water extract (16.2 %+0.3). Heavy metals (Hg, As, Cd, Pb) were within the limits given in WHO guidelines. Phytochemical screening revealed the presence of phenols, saponins, tannins, steroids, flavonoids, terpenoids and cardiac glycosides. TLC fingerprint of T. cordifolia was developed using butanol: ethyl acetate: acetic acid: water in a ratio of 5: 8: 6:2 v/v and compared with one of its marker compound, Berberine. The HPTLC fingerprint patt ern of T. cordifolia showed a spot bearing the same Rf value corresponds to Berberine, at wavelength 254 nm. In conclusion, the results obtained from this study can be used as a standard reference for Sri Lankan grown T. cordifolia stems.


2021 ◽  
Vol p5 (6) ◽  
pp. 3096-3100
Author(s):  
Akhilesh Kumar Ratre ◽  
Sandeep Shah Jureshiya ◽  
Pravin Kumar Joshi

The present study aimed at detailed pharmacognostic evaluation of the crude drug, Morpho-anatomy of the leaves of A. spinosus Linn. was studied to aid pharmacognostic and taxonomic species identification using light and con- focal microscopy, WHO suggested Physico-chemical determinations and authentic phytochemical measures. Ama- ranthus spinosus Linn. (Family Amaranthaceae), a very common Indian plant is known for its medicinal properties and is commonly known as 'spiny amaranth' or 'pig weed', ''Kate wali Chaulai (Kanatabhajii)" in 'Hindi", cultivated throughout in India, Sri Lanka and distributed throughout the tropics and warm temperate regions of Asia, it has antidiabetic, antitumor, analgesic, antimicrobial, anti-inflammatory, spasmolytic, bronchodilator, hepato-protec- tive, spermatogenic, anti-fertility, anti-malarial, antioxidant properties. The present study aims at developing a standardized profile of leaf, stem and root of A.spinosus which would be of immense use to identify and establish the authenticity of the plant A.spinosus Keywords: A Spinosus. Linn., kanta cholai, Pharmacognostical, Phytochemical


CORD ◽  
2010 ◽  
Vol 26 (2) ◽  
pp. 6
Author(s):  
K.D.P.P. Gunathilake

A study was conducted to evaluate Sri Lanka’s dikiri coconut for toffees, Dikiri spread and ice cream. The prepared products were tested for their physico-chemical and sensory properties. Initial trials were done in Sri Lanka to identify the best Dikiri kernel/sugar ratio for toffees, showing that a 1:1 ratio gave acceptable quality. Prepared Dikiri spread was compared with margarine-based bread spread. Results revealed that the product was more acceptable in terms of taste and overall acceptability compared with the margarine-spread. The Dikiri spread comprised 7.95% minerals, 11.35% crude fat, 2.68% crude protein, 4.95% crude fiber and 73.07% carbohydrate on dry weight basis. Dikiri-added ice cream was acceptable and comparable with regular dairy ice cream. Composition of the Dikiri-added ice cream conforms to Sri Lanka Standards.


Author(s):  
H. Gross ◽  
H. Moor

Fracturing under ultrahigh vacuum (UHV, p ≤ 10-9 Torr) produces membrane fracture faces devoid of contamination. Such clean surfaces are a prerequisite foe studies of interactions between condensing molecules is possible and surface forces are unequally distributed, the condensate will accumulate at places with high binding forces; crystallites will arise which may be useful a probes for surface sites with specific physico-chemical properties. Specific “decoration” with crystallites can be achieved nby exposing membrane fracture faces to water vopour. A device was developed which enables the production of pure water vapour and the controlled variation of its partial pressure in an UHV freeze-fracture apparatus (Fig.1a). Under vaccum (≤ 10-3 Torr), small container filled with copper-sulfate-pentahydrate is heated with a heating coil, with the temperature controlled by means of a thermocouple. The water of hydration thereby released enters a storage vessel.


2004 ◽  
Vol 71 ◽  
pp. 97-106 ◽  
Author(s):  
Mark Burkitt ◽  
Clare Jones ◽  
Andrew Lawrence ◽  
Peter Wardman

The release of cytochrome c from mitochondria during apoptosis results in the enhanced production of superoxide radicals, which are converted to H2O2 by Mn-superoxide dismutase. We have been concerned with the role of cytochrome c/H2O2 in the induction of oxidative stress during apoptosis. Our initial studies showed that cytochrome c is a potent catalyst of 2′,7′-dichlorofluorescin oxidation, thereby explaining the increased rate of production of the fluorophore 2′,7′-dichlorofluorescein in apoptotic cells. Although it has been speculated that the oxidizing species may be a ferryl-haem intermediate, no definitive evidence for the formation of such a species has been reported. Alternatively, it is possible that the hydroxyl radical may be generated, as seen in the reaction of certain iron chelates with H2O2. By examining the effects of radical scavengers on 2′,7′-dichlorofluorescin oxidation by cytochrome c/H2O2, together with complementary EPR studies, we have demonstrated that the hydroxyl radical is not generated. Our findings point, instead, to the formation of a peroxidase compound I species, with one oxidizing equivalent present as an oxo-ferryl haem intermediate and the other as the tyrosyl radical identified by Barr and colleagues [Barr, Gunther, Deterding, Tomer and Mason (1996) J. Biol. Chem. 271, 15498-15503]. Studies with spin traps indicated that the oxo-ferryl haem is the active oxidant. These findings provide a physico-chemical basis for the redox changes that occur during apoptosis. Excessive changes (possibly catalysed by cytochrome c) may have implications for the redox regulation of cell death, including the sensitivity of tumour cells to chemotherapeutic agents.


Author(s):  
Angela Abruzzo ◽  
Alessandra Crispini ◽  
Cecilia Prata ◽  
Rosanna Adduci ◽  
Fiore Pasquale Nicoletta ◽  
...  

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