Decoration of specific sites on freeze-fractured membranes

Author(s):  
H. Gross ◽  
H. Moor

Fracturing under ultrahigh vacuum (UHV, p ≤ 10-9 Torr) produces membrane fracture faces devoid of contamination. Such clean surfaces are a prerequisite foe studies of interactions between condensing molecules is possible and surface forces are unequally distributed, the condensate will accumulate at places with high binding forces; crystallites will arise which may be useful a probes for surface sites with specific physico-chemical properties. Specific “decoration” with crystallites can be achieved nby exposing membrane fracture faces to water vopour. A device was developed which enables the production of pure water vapour and the controlled variation of its partial pressure in an UHV freeze-fracture apparatus (Fig.1a). Under vaccum (≤ 10-3 Torr), small container filled with copper-sulfate-pentahydrate is heated with a heating coil, with the temperature controlled by means of a thermocouple. The water of hydration thereby released enters a storage vessel.

2012 ◽  
Vol 201-202 ◽  
pp. 979-982 ◽  
Author(s):  
Feng Xu ◽  
Li Xiang Zhang

Pure water hydraulic technology attracts more and more attention of people due to no pollution, low cost, simple system, and many other advantages. This paper compares physico-chemical properties between pure water and hydraulic oil, concludes that pure water is the ideal medium for hydraulic system. Then it discusses the development of pure water hydraulic technology, introduces the present research situation and development of pure water hydraulic components from home to abroad in the future.


2001 ◽  
Vol 6 (4) ◽  
Author(s):  
O. A Ashaye ◽  
A. A Couple ◽  
O.O Afolabi ◽  
S. B Fasoyiro

Author(s):  
S.H. Terde ◽  
S.R. Lande ◽  
S.S. Ramod ◽  
N.A. Suryawanshi ◽  
V.S. Dandekar

Background: Whey is the major by-product in dairy industry obtained during production of coagulated milk products like paneer, chaana, casein and cheese. Whey beverages are pure water containing sugar, flavour, edible acids and pigments and sometimes it was carbonated with carbon dioxide gas. Methods: In, present investigation kokum whey beverage was manufactured with different levels of honey and kokum syrup viz., 14 and 16 per cent level of honey and 10, 12.5 and 15 per cent level of kokum syrup incorporated with chhana whey. Result: The finished product was objected to physico-chemical analysis such as total solids, fat, protein, total sugar, ash and pH. Kokum whey beverage prepared with 14 per cent honey and 12.5 per cent kokum syrup found superior over rest of the treatments.


1975 ◽  
Vol 42 (1) ◽  
pp. 43-56 ◽  
Author(s):  
M. C. T. Hoynes ◽  
P. F. Fox

SummaryThe principal results of an investigation of the major physico-chemical properties of porcine milk were:Heat stability: all individual porcine milks examined had poor heat stability and some coagulated in 2 min at 95°C; the Q10°C was high and variable and was 40 for some samples. Dialysis of porcine milk against bulk bovine milk did not influence its heat stability; removal of colloidal calcium phosphate (CCP) from porcine milk increased its heat stability 4-fold, but it was still very unstable. Unlike bovine milk, porcine milk did not exhibit a maximum-minimum in the heat stability-pH curve, but rather resembled bovine casein micelles suspended in milk dialysate. Addition of isolated bovine β-lactoglobulin to porcine milk did not influence its heat stability-pH curve.Rennet coagulation: under similar conditions, porcine milk coagulated about 4 times faster than bovine milk and the resultant curd was about twice as soft. Dialysis of porcine milk against a large excess of bulk bovine milk did not influence its rennet coagulability nor did removal of 50% of its CCP, but complete removal of the CCP rendered porcine milk non-coagulable by rennin. Porcine milk was readily coagulated by rennin down to 5°C; its second-stage coagulation was independent of temperature – a characteristic due mainly to its high level of CCP.Alcohol stability: porcine milk was coagulated by 66–68% (v/v) ethanol, as compared with 74% (v/v) for bovine milk.Calcium stability: porcine milk was stable to at least 300 mM-CaCl2 at both 2 and 37°C.Solvation: porcine milk contained significantly less water of hydration than bovine milk, 1·7g H2O/g casein compared with 2·0g H2O/g casein.


Author(s):  
Sandra Pinto ◽  
Michelle Sanchez Aguiar ◽  
Elizabeth Hurtado ◽  
Leoni Villano Bonamin

Introduction Silicea terra 200cH can modulate macrophage activity in mice inducing resolution of chronic wounds, whose mechanisms are unknown. Herein, we describe the relation between the activity of Silicea terra 200cH in vitro, on macrophages infected with Calmette-Guérin Bacilli (BCG) - a microorganism involved in chronic granuloma lesions - and physico-chemical properties of the remedy. Objective Establish the relation between physico-chemical properties and biological activity of Silicea terra 200cH in vitro. Methods BCG-infected RAW 264.7 macrophages were analyzed considering their capacity of bacilli internalization, lysosome activity, hydrogen peroxide (H2O2) and cytokines production, using a CBA (BD) kit. The vehicle (succussed water) was used as control. The electrical conductivity and the presence of micro particles in the remedy were also studied. Results Silicea terra 200cH showed significant reduction of hydrogen peroxide production (p?0.001), higher lysosome activity (p?0.001) and increase of IL-10 production (p?0.05), an anti-inflammatory cytokine, in relation to the control. The electrical conductivity of Silicea terra 200cH was statistically higher in relation to pure water (p


Membranes ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 446
Author(s):  
Ramona Kuhn ◽  
Carsten Vornholt ◽  
Volker Preuß ◽  
Isaac Mbir Bryant ◽  
Marion Martienssen

Aminophosphonates such as aminotris(methylenephosphonic acid) (ATMP) are common constituents of antiscalants. In nanofiltration (NF) and reverse osmosis (RO) processes, ATMP prevents inorganic scaling leading to more stable membrane performance. So far, little attention has been paid to the possible permeation of aminophosphonates through NF and RO membranes. We have investigated the permeability of these membrane types for ATMP and its potential metabolites iminodi(methylenephosphonic acid) (IDMP) and amino(methylenephosphonic acid) (AMPA) with two different NF membranes (TS40 and TS80) and one RO membrane (ACM2) and three different water compositions (ultra-pure water, synthetic tap water and local tap water). We found traces of phosphonates in all investigated permeates. The highest phosphonate rejection occurred with local tap water for all three membranes investigated. Filtration experiments with a technical antiscalant formulation containing ATMP indicated similar trends of phosphonate permeability through all three membranes. We assume that the separation mechanisms of the membranes are the results of a very complex relationship between physico-chemical properties such as Donnan exclusion, feed pH, feed ionic strength and feed concentration, as well as solute–solute interactions.


1990 ◽  
Vol 63 (03) ◽  
pp. 499-504 ◽  
Author(s):  
A Electricwala ◽  
L Irons ◽  
R Wait ◽  
R J G Carr ◽  
R J Ling ◽  
...  

SummaryPhysico-chemical properties of recombinant desulphatohirudin expressed in yeast (CIBA GEIGY code No. CGP 39393) were reinvestigated. As previously reported for natural hirudin, the recombinant molecule exhibited abnormal behaviour by gel filtration with an apparent molecular weight greater than that based on the primary structure. However, molecular weight estimation by SDS gel electrophoresis, FAB-mass spectrometry and Photon Correlation Spectroscopy were in agreement with the theoretical molecular weight, with little suggestion of dimer or aggregate formation. Circular dichroism studies of the recombinant molecule show similar spectra at different pH values but are markedly different from that reported by Konno et al. (13) for a natural hirudin-variant. Our CD studies indicate the presence of about 60% beta sheet and the absence of alpha helix in the secondary structure of recombinant hirudin, in agreement with the conformation determined by NMR studies (17)


1963 ◽  
Vol 79 (2) ◽  
pp. 263-293 ◽  
Author(s):  
E.M. Savitskii ◽  
V.F. Terekhova ◽  
O.P. Naumkin

2017 ◽  
Vol 13 (3) ◽  
pp. 81-94
Author(s):  
Tariq A. AL- Dhahir ◽  
◽  
Nabeel A. Bakr ◽  
Saja B. Mohammed

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