scholarly journals УЛЬТРАМІКРОСКОПІЧНІ ПРОЯВИ У СТРУКТУРІ ПІДНИЖНЬОЩЕЛЕПНОЇ СЛИННОЇ ЗАЛОЗИ НАПРИКІНЦІ 4-ГО ТИЖНЯ ЕКСПЕРИМЕНТАЛЬНОГО ОПІОЇДНОГО ВПЛИВУ

2020 ◽  
pp. 3-6
Author(s):  
Mykhalevych Marta
Keyword(s):  
Endoplasmic Reticulum ◽  
Salivary Gland ◽  
Experimental Research ◽  
Smooth Endoplasmic Reticulum ◽  
Secretory Granules ◽  
Acinar Cells ◽  
Secretory Protein ◽  
Ultrastructural Changes ◽  
Submandibular Salivary Gland ◽  
Apical Area

This publication demonstrates the ultrastructural changes of the submandibular salivary gland under the opioid effect at the 4th week of the experimental research. After 28 days of experimental opioid effect, we found that in exocrinocytes of the end secretory departments of acinar cells revealed the destruction of organelles, especially granular and smooth endoplasmic reticulum, mitochondria, as well as reducing the content of secretory granules in the apical area of glandulocytes. In some exocrinocytes, as a result of the increase in destructive changes, a decrease in the volume of the nucleus was noted, which moved to the peripheral parts of the cell. Necrotic changes developed in individual exocrinocytes of the final secretory protein divisions. In such cells, the nucleus was reduced in volume, filled with heterochromatin.

scholarly journals УЛЬТРАМІКРОСКОПІЧНІ ПРОЯВИ У СТРУКТУРІ ПІДНИЖНЬОЩЕЛЕПНОЇ СЛИННОЇ ЗАЛОЗИ НАПРИКІНЦІ 2-ГО ТИЖНЯ ЕКСПЕРИМЕНТАЛЬНОГО ОПІОЇДНОГО ВПЛИВУ

World Science ◽  
2020 ◽  
Vol 1 (4(56)) ◽  
pp. 4-7
Author(s):  
Mykhalevych Marta
Keyword(s):  
Endothelial Cells ◽  
Endoplasmic Reticulum ◽  
Salivary Gland ◽  
Connective Tissue ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Acinar Cells ◽  
Ultrastructural Changes ◽  
Submandibular Salivary Gland ◽  
Perivascular Edema

This publication demonstrates the ultrastructural changes of the submandibular salivary gland under the opioid effect at the 2nd week of the experimental research.After 14 days of experimental opioid effect, we found the destructive changes in the organelles of exocrinocytes of acinar cells, as well as dyscirculatory processes. Capillary lumen was enlarged, overflowing with red blood cells. In the endothelium of the capillaries also noted the expansion of the granular endoplasmic reticulum. Small vacuoles appeared in the cytoplasm of endothelial cells. Single mitochondria located in the area of the endothelial cytoplasmic organelles are swollen. Due to the development of perivascular edema, the underlying connective tissue substance around the capillaries was impregnated with electron-illuminated transudate masses.

scholarly journals Morphologic Characteristics of the Submandibular Salivary Gland of the Collared Peccary (Tayassu Tajacu)

2018 ◽  
Vol 35 (02) ◽  
pp. 116-121
Author(s):  
Gabriela de Souza Reginato ◽  
Cristina de Sousa Bolina ◽  
Moacir Franco Oliveira ◽  
Sonia Regina Yokomizo Almeida ◽  
Ii-sei Watanabe ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Salivary Gland ◽  
Salivary Glands ◽  
Secretory Granules ◽  
Morphological Characteristics ◽  
Acinar Cells ◽  
Collagen Fibers ◽  
Submandibular Salivary Gland ◽  
Ductal Cells ◽  
Collared Peccary

Introduction Most salivary glands is located on the inside and around the oral cavity, and are divided into major and minor salivary glands. The aim of the present study was to describe the structural and ultrastructural morphological characteristics of the lingual tissue of the submandibular glands of the collared peccary (Tayassu tajacu). Materials and Methods The submandibular glands (n = 10) of adult male collared peccaries ( T. tajacu) were used for histological and ultrastructural analysis. The techniques used were light microscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Results The submandibular salivary glands of the collared peccary (T. tajacu) showed a capsule formed by a connective tissue containing the acinus and duct cells. Histologically, the nuclei located at the basal region of the cells was observed. The light polarized microscopy clearly showed the presence of type I and type III collagen. In the SEM image, the submandibular salivary gland revealed a round aspect separated in several lobules with bundles of collagen fibers. The vibratome sections showed the groupings of acinar cells, with intermingled secretory ducts containing vessels of different diameters. The secretory granules were noted in the apical portion of the acinar and ductal cells. The thick bundles of collagen fibers formed a glandular capsule and were identified around of the acinar and ductal cells in three-dimensional SEM images. The TEM images showed a number of secretory granules, especially in the apical region of the cytoplasm of the acinar cells and in the basal portion of the nuclei. The granular endoplasmic reticulum area, the euchromatic nuclei and the cytoplasmic projections may be seen. Mucous acinar cells separated by fine collagen fibers were also observed. Conclusion The morphological characteristics of the submandibular gland of the collared peccary is similar to that of other mammals with the same eating habits and habitat.

Mechanisms Underlying Leakage of Calcium from the Endoplasmic Reticulum of Acinar Cells of the Submandibular Salivary Gland

2005 ◽  
Vol 37 (4) ◽  
pp. 296-302
Author(s):  
O. V. Kopach ◽  
I. A. Kruglikov ◽  
P. G. Kostyuk ◽  
N. V. Voitenko ◽  
N. V. Fedirko
Keyword(s):  
Endoplasmic Reticulum ◽  
Salivary Gland ◽  
Acinar Cells ◽  
Submandibular Salivary Gland

Ultrastructural Changes of Smoooth Endoplasmic Reticulum in the Retinal Pigment Epithelium by Choline Chloride

1972 ◽  
Vol 30 ◽  
pp. 58-59
Author(s):  
Kazushige Hirosawa ◽  
Eichi Yamada
Keyword(s):  
Endoplasmic Reticulum ◽  
Epithelial Cell ◽  
Smooth Endoplasmic Reticulum ◽  
Pigment Epithelium ◽  
Choline Chloride ◽  
Retinal Pigment ◽  
Ultrastructural Changes ◽  
Lower Vertebrate ◽  
Visual Cells ◽  
Pigment Epithelial

The pigment epithelium is located between the choriocapillary and the visual cells. The pigment epithelial cell is characterized by a large amount of the smooth endoplasmic reticulum (SER) in its cytoplasm. In addition, the pigment epithelial cell of some lower vertebrate has myeloid body as a specialized form of the SER. Generally, SER is supposed to work in the lipid metabolism. However, the functions of abundant SER and myeloid body in the pigment epithelial cell are still in question. This paper reports an attempt, to depict the functions of these organelles in the frog retina by administering one of phospholipid precursors.

A Morphometric Analysis of Early Effects of Ethanol on Hepatocyte Organelles from Peromyscusmaniculatus

1998 ◽  
Vol 4 (S2) ◽  
pp. 1064-1065
Author(s):  
J. T. Ellzey ◽  
J. P. Drake ◽  
L. Dader ◽  
P. Boentges
Keyword(s):  
Endoplasmic Reticulum ◽  
South Carolina ◽  
Morphometric Analysis ◽  
Smooth Endoplasmic Reticulum ◽  
Ultrastructural Changes ◽  
Deer Mice ◽  
Pathological Changes ◽  
Genetic Stock ◽  
Early Effects ◽  
Hanta Viruses

Pathological changes of hepatocytes from rats fed a 30% ethanol-derived calories diet for three weeks include noticeable ultrastructural changes including steatosis and hypertrophy of the smooth endoplasmic reticulum. We sought to examine hepatocytes of deer mice administered ethanol in an inhalation chamber for two weeks to determine if subtle changes occur in hepatocyte organelles prior to steatosis.Two strains of Peromyscus maniculatus, ADH-positive possessing hepatic cytosolic alcohol dehydrogenase and ADH-negative deer mice lacking this enzyme were purchased from the Peromyscus Genetic Stock Center (Univ. of South Carolina). They tested negatively for Hanta viruses. A morphometric analysis of the ultrastructure of ADH+(n=14) and ADH- (n=14) controls as well as experimentals exposed to chronic, intoxicating levels of ethanol was conducted. Blood ethanol levels were maintained between 1.25-1.75 mg/ml for two weeks in the experimentals.

scholarly journals Relationship between the golgi apparatus, gerl, and secretory granules in acinar cells of the rat exorbital lacrimal gland

1977 ◽  
Vol 74 (2) ◽  
pp. 399-413 ◽  
Author(s):  
AR Hand ◽  
C Oliver
Keyword(s):  
Endoplasmic Reticulum ◽  
Electron Microscope ◽  
Golgi Apparatus ◽  
Lacrimal Gland ◽  
Secretory Granules ◽  
Acinar Cells ◽  
Secretory Proteins ◽  
Thiamine Pyrophosphatase ◽  
Variable Distance ◽  
Secretory Enzyme

The method of secretory granuleformation in the acinar cells of the rat exorbital lacrimal gland was studied by electron microscope morphological and cytochemical techniques. Immature secretory granules at the inner face of the Golgi apparatus were frequently attached to a narrow cisternal structure similar to GERL as described in neurons by Novikoff et al. (Novikoff, P. M., A. B. Novikoff, N. Quintana, and J.-J. Hauw. 1971. J. Cell Bio. 50:859-886). In the lacrimal gland. GERL was located adjacent to the inner Golgi saccule, or separated from it by a variable distance. Portions of GERL were often closely paralleled by modified cisternae of rough endoplasmic reticulum (RER), which lacked ribosomes on the surface adjacent to GERL. Diaminobenzidine reaction product of the secretory enzyme peroxidase was localized in the cisternae of the nuclear envelope, RER, peripheral Golgi vesicles, Golgi saccules, and immature and mature secretory granules. GERL was usually free of peroxidase reaction product or contained only a small amount. Thiamine pyrophosphatase reaction product was present in two to four inner Golgi saccules; occasionally, the innermost saccule was dilated and fenestrated, and contained less reaction product than the next adjacent saccule. Acid phosphatase (AcPase) reaction product was present in GERL, immature granules, and, rarely, in the innermost saccule, but not in the rest of the Golgi saccules. Thick sections of AcPase preparations viewed at 100 kV revealed that GERL consisted of cisternal, and fenestrated or tublular portions. The immature granules were attached to GERL by multiple connections to the tublular portions. These results suggest that, in the rat exorbital lacrimal gland, the Golgi saccules participate in the transport of secretory proteins, and that GERL is involved in the formation of secretory granules.

scholarly journals Tmem16AEncodes the Ca2+-activated Cl−Channel in Mouse Submandibular Salivary Gland Acinar Cells

2010 ◽  
Vol 285 (17) ◽  
pp. 12990-13001 ◽  
Author(s):  
Victor G. Romanenko ◽  
Marcelo A. Catalán ◽  
David A. Brown ◽  
Ilva Putzier ◽  
H. Criss Hartzell ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Acinar Cells ◽  
Submandibular Salivary Gland ◽  
Cl Channel

scholarly journals Ultrastructural Changes in the Liver of Intravenous Heroin Addicts

2010 ◽  
Vol 10 (1) ◽  
pp. 38-43 ◽  
Author(s):  
Goran Ilić ◽  
Radovan Karadžić ◽  
Lidija Kostić-Banović ◽  
Jovan Stojanović ◽  
Aleksandra Antović
Keyword(s):  
Endoplasmic Reticulum ◽  
Plasma Membrane ◽  
Liver Tissue ◽  
Smooth Endoplasmic Reticulum ◽  
Decisive Role ◽  
Basal Membrane ◽  
Ultrastructural Changes ◽  
Heroin Addicts ◽  
Transmission Electron ◽  
Ultrastructural Findings

The ultrastructural research has a decisive role in gathering the knowledge on the liver’s response to the influence of some drugs. The aim of the study was to perform an ultrastructurai analysis of the liver in chronic intravenous heroin addicts.The study involved the autopsy conducted on 40 bodies of intravenous heroin addicts and 10 control autopsies. The liver tissue was fixed in glutaraldehyde and moulded with epon for investigation purposes of ultrastructural changes. The analysis was performed using the method of transmission electron microscopy.In the group of intravenous heroin addicts, the liver autopsy samples showed degenerative vesicular and fat changes, chronic active and persistent hepatitis, cirrhosis, reduction in the amount of glycogen in hepatocytes, as well as the Kupffer cell’s dominant hypertrophy. Various changes occur in organelles, plasma membrane of hepatocytes and biliary channels as well as in the nucleus.The most important ultrastructural findings include: hyperplasia and hypertrophy of the smooth endoplasmic reticulum, which is histologically proven vesicular degeneration of hepatocyte occurring as a result of the increased synthesis of enzymes of smooth endoplasmic reticulum due to chronic intravenous heroin intake, and the presence of continuous basal membrane followed by transformation of the sinusoids into capillaries (in the cases of chronic active hepatitis and cirrhosis) which leads to a disorder of microcirculation and further progress of cirrhosis.

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