scholarly journals A PCR Method to Detect Myxobolus acanthogobii (Myxozoa: Myxosporea), the Causative Agent of Skeletal Deformities of Marine Fishes

2005 ◽  
Vol 40 (4) ◽  
pp. 197-199
Author(s):  
Seiji Miyajima ◽  
Hiroshi Yokoyama ◽  
Yutaka Fukuda ◽  
Kumiko Okamoto ◽  
Kazuo Ogawa
Keyword(s):  
Causative Agent ◽  
Marine Fishes ◽  
Skeletal Deformities ◽  
Pcr Method

scholarly journals Anisakiasis Pada Ikan Laut Di Indonesia: Prevalensi, Sebaran Dan Potensi Patogenitasnya Pada Manusia

2021 ◽  
Vol 14 (1) ◽  
pp. 11
Author(s):  
Forman Erwin Siagian ◽  
Esy Maryanti
Keyword(s):  
Causative Agent ◽  
Health Problem ◽  
Clinical Manifestations ◽  
Review Article ◽  
Marine Fishes ◽  
Predisposing Factor ◽  
Nematode Worm ◽  
Global Data

Nematode worm Anisakis sp is the causative agent of anisakiasis, a zoonosis which continues to be a health problem in humans, globally. Its clinical spectum varies from mild to very severe and always connected with sea fishes, one important source of nutrition for the community that cannot be ignored. In addition, consumer’s behavior and lifestyle in processing and consuming sea fishes also have has the potency as the predisposing factor for its transmission and clinical manifestations. This review article aimed to describes recent global data on anisakiasis and its occurrence in marine fishes of Indonesian waters and the risk of transmission in the community.

Asymptomatic iridovirus infection in various marine fishes detected by a 2-step PCR method

Aquaculture ◽  
2006 ◽  
Vol 255 (1-4) ◽  
pp. 30-38 ◽  
Author(s):  
Joon Bum Jeong ◽  
Lyu Jin Jun ◽  
Kyung Hyun Park ◽  
Ki Hong Kim ◽  
Joon-Ki Chung ◽  
...  
Keyword(s):  
Marine Fishes ◽  
Pcr Method

scholarly journals Prevalence of Kudoa in Fish Fillets Caught in Para State

2020 ◽  
Vol 2 (3) ◽  
Author(s):  
Marianna Vaz RODRIGUES ◽  
Patrícia Tidori MIURA ◽  
Jéssica Fernandes De OLIVEIRA ◽  
Maria das Dores Correia Palha ◽  
João Pessoa Araújo JÚNIOR
Keyword(s):  
Causative Agent ◽  
Economic Cost ◽  
Marine Fishes ◽  
Molecular Techniques ◽  
Dystrophic Calcification ◽  
Coagulative Necrosis ◽  
Fish Fillets ◽  
Muscle Lesions

Kudoa is a myxozoan that causes myoliquefaction in marine fishes. Most of species only affect fish, but a K. septempunctata outbreak was reported in 358 people. Although many species of Kudoa are known, none was described in Brachyplatystoma filamentosum, Brachyplatystoma rousseauxii, Mugil curema, Plagioscion squamosissimus or Oxydoras niger until now. Due to the economic cost of eliminating seafood presenting myxozoan lesions, this study aimed to describe lesions found at necropsy and histopathology, as well as to detect this myxozoan by molecular techniques. For this purpose, were sampled 85 fish of the following species: Brachyplatystoma filamentosum, Brachyplatystoma rousseauxii, Mugil curema, Plagioscion squamosissimus, and Oxydoras niger from Colares and Vigia, Pará, Brazil. Necropsies were carried out to describe lesions and molecular techniques (PCR and sequencing) were applied for identification. Although muscle lesions were not observed at necropsy, histopathology revealed bacterial colonies, coagulative necrosis, dystrophic calcification, eosinophils, hemorrhage, parasitic cysts, protozoan, and vacuolization. After sequencing, K. shiomitsui (GENBANK: LC128646) was identified as the causative agent of fishes infection. Also, high parasitism of this myxozoan was observed in fishes sampled, i.e., 90 % in Colares and 100% in Vigia.

Scanning electron microscopy of erythrophagocytosis by Entamoeba histolytica trophozoites

1992 ◽  
Vol 50 (1) ◽  
pp. 880-881
Author(s):  
Victor Tsutsumi ◽  
Adolfo Martinez-Palomo ◽  
Kyuichi Tanikawa
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Red Blood Cells ◽  
Entamoeba Histolytica ◽  
Causative Agent ◽  
Blood Cells ◽  
Protozoan Parasite ◽  
Complex Phenomenon ◽  
Great Capacity ◽  
Scanning Electron

The protozoan parasite Entamoeba histolytica is the causative agent of amebiasis in man. The trophozoite or motile form is a highly dynamic and pleomorphic cell with a great capacity to destroy tissues. Moreover, the parasite has the singular ability to phagocytize a variety of different live or death cells. Phagocytosis of red blood cells by E. histolytica trophozoites is a complex phenomenon related with amebic pathogenicity and nutrition.

Investigation of duck plague virus in hoar areas of Bangladesh

2020 ◽  
Vol 26 (1-2) ◽  
pp. 73-78
Author(s):  
A Hossen ◽  
MH Rahman ◽  
MZ Ali ◽  
MA Yousuf ◽  
MZ Hassan ◽  
...  
Keyword(s):  
Cytopathic Effect ◽  
Clinical Sample ◽  
Chorioallantoic Membrane ◽  
Clinical Samples ◽  
Isolation And Identification ◽  
Duck Plague Virus ◽  
Pcr Method ◽  
Polymerase Chain ◽  
The Individual ◽  
Free Ranging

Duck plague (DP) is the most important infectious disease of geese, ducks and free-ranging water birds. The present study was conducted to determine the prevalence of duck plague virus followed by isolation and identification. For these purposes, a total of 155 cloacal swabs samples were collected randomly from duck of different haor areas of Bangladesh including 45 (41 surveillance and 4 clinical) samples from Netrokona; 42 (40 surveillance and 2 clinical) samples from Kishoregonj; 30 samples from Brahmanbaria and 38 samples from Sunamganj. The samples were processed and pooled (1:5 ratio) for initial screening of target polymerase gene of duck plague virus by polymerase chain reaction (PCR) method. All the samples of a positive pool were then tested individually for identifying the individual positive samples. The result showed that out of 155 samples, 41 (26.45%) were found positive in which 17 were from Netrokona, where 15 (36.58%) were from surveillance samples and 2 (50%) were from clinical sample; 16 were from Kishoregonj, where 14 (35%) were from surveillance samples and 2 (100%) were from clinical sample; 2 (6.6%) were from Brahmanbaria and 5 (13.15%) were from Sunamganj. These positive samples were inoculated into 9-10 days embryonated duck eggs (EDE) through chorioallantoic membrane (CAM) route for the isolation of virus. The EDE died earlier was also chilled, and in a similar way, the CAMs were collected and again performed PCR for id entification of virus. Out of 41 PCR positive samples, 26 samples were isolated and reconfirmed by PCR. Subsequently, DPV was isolated in primary duck embryo fibroblasts cell culture and confirmed by observing cytopathic effect (CPE). Bang. J. Livs. Res. Vol. 26 (1&2), 2019: P. 73-78

Assessment of stock vulnerability of Indian marine fishes to past changes in climate and options for adaptation

2020 ◽  
Vol 79 (3) ◽  
pp. 175-192
Author(s):  
AP Dineshbabu ◽  
PU Zacharia ◽  
T Sujitha ◽  
JK Shoba ◽  
KM Rajesh ◽  
...  
Keyword(s):  
Marine Fishes
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