scholarly journals Molecular Identification of Marine Crustacean-pathogenic Peronosporomycetes Using DNA Sequences of ITS1 and their Pathogenicity for Nauplii of Brine Shrimps

2012 ◽  
Vol 47 (2) ◽  
pp. 41-48 ◽  
Author(s):  
Yasunori Muraosa ◽  
Ayako Sano ◽  
Kishio Hatai
Keyword(s):  
Molecular Identification ◽  
Dna Sequences ◽  
Brine Shrimps ◽  
Marine Crustacean

scholarly journals An updated checklist of the lichens of St. Eustatius, Netherlands Antilles

MycoKeys ◽  
2018 ◽  
Vol 33 ◽  
pp. 69-84 ◽  
Author(s):  
Andre Aptroot ◽  
Michael Stech
Keyword(s):  
Molecular Identification ◽  
Dna Sequences ◽  
Species Level ◽  
Lichen Species ◽  
Vegetation Types ◽  
Netherlands Antilles ◽  
Phylogenetic Studies ◽  
Biodiversity Inventory ◽  
Unidentified Species ◽  
Chemical Characters

In the course of a multi-taxon biodiversity inventory for the island of St. Eustatius, lichens were collected from 11 plots representing different vegetation types. From these collections, 126 lichen species are reported, 54 of which are new reports for St. Eustatius. Most species could be identified to species level based on morphological and chemical characters. In a few cases, mtSSU DNA sequences were generated for a preliminary molecular identification and future phylogenetic studies. In total, 263 identified lichen species are currently known from St. Eustatius, as well as some additional genera with yet unidentified species and lichenicolous fungi.

scholarly journals AN INTEGRATIVE TAXONOMY OF MOLECULAR AND TRADITIONAL APPROACHES FOR IDENTIFICATION OF A STORAGE MITE, ALEUROGLYPHUS OVATUS (ACARI: ASTIGMATA: ACARIDAE) IN MALAYSIA

2016 ◽  
Vol 6 (2) ◽  
pp. 882-889
Author(s):  
Ernieenor Faraliana Che Lah
Keyword(s):  
Molecular Identification ◽  
Dna Sequences ◽  
Correct Diagnosis ◽  
Morphological Characters ◽  
Integrative Taxonomy ◽  
Its2 Region ◽  
Morphological Identification ◽  
Taxonomic Identification ◽  
Internal Transcribed Spacer Region ◽  
Accurate Identification

A reliable and rapid taxonomic identification of a mite is the basis for a correct diagnosis of important mite associated allergies as they produce species-specific allergens. A double approach (molecular and morphological) to the taxonomic identification of Aleuroglyphus ovatus was presented. Molecular identification was performed with amplification of the internal transcribed spacer region (ITS2), whilst morphological characters were examined under light microscope. The BLAST results obtained from molecular analysis of A. ovatus was shown to be in concordance with the morphological identification with 97% genetic similarity. Thus, the molecular identification based on the ITS2 region can be applied as a reliable and efficient tool for species identification of Aleuroglyphus and probably any other astigmatid mites. Our findings suggest the need for a broad taxonomic sampling especially from closely related species for an accurate identification of local mites using both DNA sequences and morphology.

scholarly journals Molecular identification of genus Pipistrellus (Mammalia: Chiroptera) from Fata region, Pakistan

2023 ◽  
Vol 83 ◽  
Author(s):  
M. Idnan ◽  
A. Javid ◽  
M. Tayyab ◽  
A. Hussain ◽  
S. Mansoor ◽  
...  
Keyword(s):  
New Species ◽  
16S Rrna ◽  
Molecular Identification ◽  
Dna Sequences ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Morphometric Measurements ◽  
Tree Analysis ◽  
Rrna Sequences ◽  
16S Rrna Sequences

Abstract A total of 10 specimens were captured from selected sites of Bajaur Agency FATA, Pakistan using mist nets. The captured specimens were morphologically identified and various morphometric measurements were taken. The head and Body length (HB) of Pipistrellus coromondra and Pipistrellus kuhlii lepidus (n=10) was 43±0.11 mm and 45±1.1 respectively. Morphologically identified Pipistrellus kuhlii confirmed as Pipistrellus kuhlii lepidus based on 16S rRNA sequences. The DNA sequences were submitted to GenBank and accession numbers were obtained (MN 719478 and MT430902). The available 16S rRNA gene sequences of Pipistrellus coromondra and Pipistrellus kuhlii lepidus were retrieved from NCBI and incorporated in N-J tree analysis. Overall, the interspecific genetic variations among Pipistrellus coromondra and Pipistrellus kuhlii lepidus were 8% and 1% respectively. In our recommendation, a comprehensive molecular identification of bats is need of hour to report more cryptic and new species from Pakistan.

scholarly journals Molecular Identification of Mating Type Genes in Asexually Reproducing Fusarium Oxysporum and F. Culmorum

2011 ◽  
Vol 51 (4) ◽  
pp. 405-409 ◽  
Author(s):  
Lidia Irzykowska ◽  
Tomasz Kosiada
Keyword(s):  
Fusarium Oxysporum ◽  
Mating Type ◽  
Molecular Identification ◽  
Dna Sequences ◽  
Control Strategies ◽  
Sexual Stage ◽  
Reproduction Mode ◽  
Successful Control ◽  
Mating Type Genes

Molecular Identification of Mating Type Genes in Asexually ReproducingFusarium OxysporumandF. CulmorumSexually (homothallic and heterothallic) and asexually reproducing species belong to theFusariumgenus. So far, there is no known sexual stage of theF. oxysporumSchlechtend.: Fr. andF. culmorum(W.G. Smith) Sacc. Knowing the reproduction mode is important for the design of successful control strategies, since they are different for clonally and sexually reproducing organisms. In examined sets of asexualF. oxysporumandF. culmorumisolates, the DNA sequences of mating type genes (idiomorphsMAT-1andMAT-2) were identified.MAT-1sequence was detected for 33 and 40% ofF. oxysporumandF. culmorumisolates, respectively. For the remaining isolates a sequence specific forMAT-2was amplified.

scholarly journals Phenotypic and Molecular Identification of Pathogenic Fusarium Species Isolated from Various Pulse Growing Geographic Areas of India

2021 ◽  
Vol 10 (11) ◽  
pp. 120-132
Author(s):  
Monika Singh O. P. Sharma ◽  
Someshwar Bhagat
Keyword(s):  
Molecular Identification ◽  
Dna Sequences ◽  
Fusarium Species ◽  
Economic Loss ◽  
Colony Growth ◽  
Likelihood Method ◽  
Multiple Sequence ◽  
Colony Color ◽  
Fusarium Sp ◽  
Its Dna Sequences

Fusarium phythopathogenic fungi is responsible for high economic loss of cereal food crop. The objective of this study was aimed at isolation, morphological and molecular identification of Fusarium species. 13 different Fusarium spp. i.e. F. solani, F. chlamydosporum, F. tabacinum, F. fujikuroi, F. oxysporum, F. verticillioides, F. brachygibbosum, Fusarium sp. and F. incarnatum were isolated and identified from diseased samples of chickpea, pigeonpea, rice, lentil and garden pea crop. Colony characteristics like colony color, colony growth diameters, mycelium type, sporulation, pigmentation, odour were obtained after culture purification. Shape, size and septation of microconidia and macroconidia, position, shape, occurrence and size of chlamydospores, conidiophore branching were examined microscopically. MS10, BI01 and KA14 isolates were slow growing, BI02 and UP07 were moderate growing and BI03, HA04, MS06, MS09, MS11 and KA(Gul)13 were fast to very fast growing on PDA after 7-10 days. Chlamydospores were found in most of the isolates. Colonies were abundant, loosely tufted, fluffy, pannose, vinaceous floccose, powdery and some were flat appressed, arachnoid. Pigmentation of most of the isolates was pinkish white to dark pink, carmine to violet in colour. Phylogenetic analysis was done by maximum likelihood method using the ITS-rDNA region of Fusarium isolates and multiple sequence alignment of ITS DNA sequences was done using Clustal_W program and all identified sequences were submitted in NCBI GenBank database.

scholarly journals Morphology, molecular identification, and pathogenicity of Vibrio spp. on blood clam (Anadara granosa) in Yogyakarta, Indonesia tourism beach areas

2019 ◽  
Vol 20 (10) ◽  
Author(s):  
ANNA ROOSIANA DEVI ◽  
Ari Susilowati ◽  
RATNA SETYANINGSIH
Keyword(s):  
Molecular Identification ◽  
Dna Sequences ◽  
Pathogenic Bacteria ◽  
Morphological Characters ◽  
Foodborne Disease ◽  
16S Rrna Sequence ◽  
Blood Clam ◽  
Agar Media ◽  
Anadara Granosa ◽  
Vibrio Spp

Abstract. Devi AR, Susilowati A, Setyaningsih R. 2019. Morphology, molecular identification, and pathogenicity of Vibrio spp. on blood clam (Anadara granosa) in Yogyakarta, Indonesia tourism beach areas. Biodiversitas 20: 2890-2896. Seafood is very popular among Indonesian people, especially in coastal areas. In Bantul Yogyakarta, blood clams have become one of tourist's favorite, either cooked or raw. Blood clams are filter feeders that cause the clams to be vulnerable to contamination of pathogenic bacteria that can cause foodborne disease, including Escherichia, Pseudomonas, and Vibrio. The 10-20% cases of foodborne disease transmitted through seafood caused by Vibrio spp. Three species of Vibrio can cause foodborne disease in humans, i.e., V. cholerae, V. parahaemolyticus, and V. vulnificus. The purpose of this study was to determine the character of Vibrio using morphological and molecular identification and pathogenicity on blood clam (Anadara granosa). Blood clams samples were collected from Depok, Goa Cemara, and Kwaru beaches in Bantul, Yogyakarta, Indonesia. Isolation of Vibrio spp. from blood clams was done using selective differential Thiosulfate Citrate bile salts sucrose (TCBS) culture medium. The morphological characters of the isolate colonies were determined based on the color, shape, texture, and size of the colony. Hemolysis test was also performed to evaluate the pathogenicity by using blood agar media. Molecular identification of Vibrio species was made using 16S rRNA sequence. Phylogenetic analysis was performed using Neighbor-Joining method in Mega X software. Samples for the analysis came from DNA sequences of this study and those from the GenBank database. Of the total 15 isolates obtained, four isolates showed positive β-hemolysis, namely, isolate P2S2 -1bH, P3S1-1aH, P2S1-1aK and P2S2-1aK, and one isolate had positive α-hemolysis (P3S2-1aK). Seven species of Vibrio were identified as V. algynolyticus, V. parahaemolyticus, V. diabolicus, V neocaledonicus, V. azureus, V. natrigens, and V. cholerae.

Sign in / Sign up

Export Citation Format

Share Document