scholarly journals VERIFICATION OF VIABILITY AND PHENOTYPICAL PROPERTIES OF STRAINS INFECTIOUS AGALACTIC OF SHEEP AND GOATS

2021 ◽  
Vol 245 (1) ◽  
pp. 9-13
Author(s):  
E.A. Artemeva ◽  
◽  
L.A. Melnikova ◽  
A.P. Rodionov ◽  
◽  
...  
Keyword(s):  
Biological Properties ◽  
Industrial Complex ◽  
Optimal Conditions ◽  
Sheep And Goats ◽  
Long Term Storage ◽  
Passport Data ◽  
Prophylactic Vaccines ◽  
And Control ◽  
Term Storage

The article provides data about the first cases of detection and spread of infectious agalactia in sheep and goats, both in Russia and abroad. It was revealed that these are mainly regions with traditional sheep breeding, which include regions of Europe, Asia, Africa, as well as countries locat-ed along the shores of the Mediterranean Sea. The success of preventing and eradicating this disease depends on the availability of prophylactic vaccines and diagnostic drugs. The basis for their manu-facture is the production and control strains of the pathogen, which are obtained by biological en-terprises of the agro-industrial complex from the state collections of microorganisms. In the collec-tions, strains are studied at the frequency indicated in the passport, and optimal conditions are cre-ated to prevent microorganisms from losing their viability and their biological properties during long-term storage. The present work shows results of studies of strains A-319 and 8899 of the caus-ative agent of infectious agalactia in sheep and goats stored for 20 years, in a lyophilized form. The data obtained for determining the viability and studying the biological properties of the strains showed their compliance with the passport data.

NANOEMULSIONS BASED ON LIPOIC ACID DERIVATIVES WITH VARIOUS ALCOHOLS: PRODUCTION AND INFLUENCE ON FUNCTIONAL ACTIVITY OF PLATELETS

2020 ◽  
pp. 461-464
Author(s):  
A. Sinebryukhova ◽  
A. Shipelova ◽  
E. Darnotuk ◽  
A. Chekanov ◽  
O. Baranova ◽  
...  
Keyword(s):  
Particle Size ◽  
Arachidonic Acid ◽  
Lipoic Acid ◽  
Encapsulation Efficiency ◽  
Room Temperature ◽  
Optimal Conditions ◽  
Pluronic F68 ◽  
Long Term Storage ◽  
Term Storage

The optimal conditions were selected for obtaining homogeneous nanoemulsions (NE) of lipoic acid conjugates (LA-conjugates) based on Pluronic F68 (1,8%) with a particle size not exceeding 400 nm, characterized by 97±2% encapsulation efficiency of substances in nanoparticles (NP). A heterogeneous NE (polydispersity index, PDI>0,3) with the derivative of LA and myo-inositol based on phosphatidylcholine (PC, C = 3 mg/ml) was also obtained consisting of 2 particle fractions: 20–70 nm (27%) and 122–212 nm (73%). The obtained NEs with LA-conjugates based on Pluronic F68 and PC were stable during long-term storage (more than 12 months) at room temperature. The effect of the obtained NEs of LA-conjugates on platelet aggregation (Pt) caused by arachidonic acid (AA) was determined, and a mechanism of their action was proposed.

Development and control of scald on wonderful pomegranates during long-term storage

2006 ◽  
Vol 41 (3) ◽  
pp. 234-243 ◽  
Author(s):  
Bruno G. Defilippi ◽  
Bruce D. Whitaker ◽  
Betty M. Hess-Pierce ◽  
Adel A. Kader
Keyword(s):  
Long Term Storage ◽  
And Control ◽  
Term Storage

scholarly journals Use of Formalin-Fixed Tissues to Determine Fumonisin B1-Induced Sphingolipid Alterations in Swine

2007 ◽  
Vol 19 (4) ◽  
pp. 425-430 ◽  
Author(s):  
Shih-Hsuan Hsiao ◽  
Mike E. Tumbleson ◽  
Peter D. Constable ◽  
Wanda M. Haschek
Keyword(s):  
Pulmonary Edema ◽  
Fumonisin B1 ◽  
Formalin Fixation ◽  
Research Projects ◽  
Long Term Storage ◽  
And Control ◽  
Term Storage ◽  
Formalin Fixed

Fumonisin B1 is a mycotoxin that causes lethal pulmonary edema in swine. Sphinganine, sphingosine, and the sphinganine to sphingosine ratio are important biomarkers for fumonisin B1 exposure. Currently, tissues selected for sphinganine and sphingosine analyses are frozen at −80°C until analyses take place. However, for diagnostics and some research projects, formalin is used more routinely as a preservative for long-term storage of tissues. To determine whether formalin-fixed tissues could be used for sphinganine and sphingosine analyses, sphinganine and sphingosine concentrations were quantified in both frozen and formalin-fixed lung, liver, kidney, and heart from fumonisin B1-treated and control pigs. Tissues were evaluated 3 months after freezing and 3, 6, and 12 months after formalin fixation. Sphinganine, sphingosine, and the sphinganine to sphingosine ratio of both frozen and formalin-fixed lung and liver from fumonisin B1-treated pigs were elevated. Formalin-fixed tissues had lower sphinganine and sphingosine concentrations but higher sphinganine to sphingosine ratios than the corresponding frozen tissues. Storage in formalin for up to 12 months did not affect the results. Sphingosine analysis could not be performed in formalin-fixed heart and kidney because of noninterpretable chromatograms. Therefore, formalin-fixed lung and liver can be used to determine fumonisin B1-induced sphinganine and sphingosine alterations in swine, with the sphinganine to sphingosine ratio being the most useful.

scholarly journals (52) Postharvest Alleviation of Chilling Injury and Diseases of Cantaloupe Melons

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1088B-1088
Author(s):  
Christian Krarup ◽  
Oscar Núñez
Keyword(s):  
Chilling Injury ◽  
Disease Response ◽  
Long Term Storage ◽  
Heat Treated ◽  
Highly Sensitive ◽  
Postharvest Life ◽  
And Control ◽  
Term Storage ◽  
Main Factor

Chilling injury of cantaloupe melons (Cucumismelo, Cantalupensis group), although variable among cultivars, precludes the use of temperatures close to 0 °C for long term storage or transport. Diseases, either associated with chilling or independent from it, are usually the main factor terminating postharvest life of these melons. `Colima', a Western Shipper cultivar highly sensitive to chilling, was used to evaluate chilling and disease response to the following treatments: immersion in water at 60 °C for 1 minute (with or without 150 ppm chlorine), individual packaging (PVC, whole or perforated), exposure to air at 38 °C for 12 hours (with or without individual packaging), and control. After storage for 18 days at 0 °C and a simulated retail period of 3 days at 20 °C, there were significant differences among treatments: chilling was alleviated in heat-treated and especially in plastic-wrapped fruit, and the presence of diseases caused by pathogens such as Alternaria, Cladosporium, Fusarium, and Rhizopus was markedly reduced by heat treatments. Overall, visual quality was high and superior in fruit immersed in water at 60 °C with 150 ppm chlorine due to almost complete supression of diseases. Project financed by FONDECYT 1020882.

scholarly journals EFFECT OF VARIOUS CRYOPROTECTANTS ON PRESERVATION OF BLACK TIGER Penaeus monodon SHRIMP SPERMATOZOA

2021 ◽  
Vol 15 (2) ◽  
pp. 77
Author(s):  
Rosmiati Rosmiati ◽  
Ike Trismawanti ◽  
Samuel Lante
Keyword(s):  
Saline Solution ◽  
Penaeus Monodon ◽  
Sperm Viability ◽  
Electrical Shock ◽  
Long Term Storage ◽  
Tiger Shrimp ◽  
Time Period ◽  
And Control ◽  
Term Storage

The development of cryopreservation technique on tiger shrimp Penaeus monodon broodstock spermatophore has been carried out to support the artificial insemination. This study aims to determine the effect of three cyoprotectants (methanol, dimetylsulphoxide (DMSO), and glycerol) for long term storage of tiger shrimp Penaeus monodon spermatozoa. Spermatophores were collected from the wild broodstocks through electrical shock. Spermatozoa were obtained by homogenizing the spermatophores using a Radnoti micro homogenizer in Ca-free saline solution containing one of three cryoprotectans (methanol, dimetylsulphoxide, and glycerol) separately at the concentration of 5%. One mL of each cryoprotectant containing spermatozoa with the density of 1.02 x 106 cell/mL was transferred into a cryovial and cryopreserved at room temperature, -20°C and -196°C for 5, 10, and 30 days. The apparent sperm viability (ASV) of cryopreserved spermatozoa was monitored after treated. Thawing of cryopreserved spermatozoa was carried out in a 30°C wáter bath for two minutes. The result showed that the best apparent sperm viability was obtained at the using of glycerol at -196°C in liquid nitrogen, even after the thirty days of cryopreservation time period with the ASV of 0.82 x 106 cells/mL (80.39%). Meanwhile two other cryoprotectans displayed the ASV of 0.54 x 106 cells/mL (56.86%), and 0.23 x 106 cells/mL (22.55%). for DMSO and methanol, respectively. In turn, the control showed the lowest ASV with the ASV of 0.01 x 106 cells/mL (1.27%). The ASV showed by this glycerol exhibited asignificant difference (P<0.05) to that of methanol, DMSO, and control.

scholarly journals Alginate-, Carboxymethyl Cellulose-, and κ-Carrageenan-Based Microparticles as Storage Vehicles for Cranberry Extract

Molecules ◽  
2020 ◽  
Vol 25 (17) ◽  
pp. 3998
Author(s):  
Marta Tsirigotis-Maniecka
Keyword(s):  
Sodium Alginate ◽  
Plant Extract ◽  
Carboxymethyl Cellulose ◽  
Encapsulation Efficiency ◽  
Biological Properties ◽  
Safe Delivery ◽  
Long Term Storage ◽  
Term Storage

This study discusses the relationship between the structural properties of the selected polysaccharides (low (ALGLV) and medium viscosity (ALGMV) sodium alginate, 90 kDa (CMC90) and 250 kDa (CMC250) carboxymethyl cellulose, and κ-carrageenan (CARκ)) and their abilities to serve as protective materials of encapsulated large cranberry (Vaccinium macrocarpon Aiton) fruit extract (CE) from losing its health beneficial activities during long-term storage. The microparticles were characterized in terms of their encapsulation efficiency (UV-Vis and FTIR), morphology (SEM) and the physical stability in various environments (gravimetry). The microparticles’ size and encapsulation efficiency were 46–50 µm and 28–58%, respectively, and the microparticles were physically stable. CMC90 and ALGMV most efficiently protected the plant extract from losing its biological activity after 18 months, while the plant extract stored outside the particles had lost its activity. CE was intended for oral administration, thus CE release from the microparticles was monitored in vitro under gastrointestinal conditions. In vitro gastrointestinal release studies revealed that the ALGMV-, CMC90-, and CMC250-based particles exhibited the desired intestinal release pattern. This result supports the suitability of sodium alginate and carboxymethyl cellulose for the safe delivery of CE to the intestines while maintaining its biological properties and improving long-term storage stability.

scholarly journals The Importance Of Lyophilization Of Microorganisms And Biologicals

2021 ◽  
Vol 03 (07) ◽  
pp. 46-52
Author(s):  
Okiev S.S. ◽  
Keyword(s):  
Scientific Research ◽  
Biological Properties ◽  
Long Term Storage ◽  
The Stability ◽  
Term Storage

This article provides arguments about the need for scientific research aimed at maintaining the stability of biological properties during long-term storage, production, reference, epizootic strains of microorganisms through the development and optimization of lyophilization parameters.

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