QUALITATIVE AND QUANTITATIVE ANALYSIS OF FLAVONOIDS DURING DEVELOPMENT OF ANTIDIABETIC EXTRACT

Author(s):  
D. Aleksandrova ◽  
A. Legosteva

During development of antidiabetic extract in blueberry shoots (Vaccinium myrtillus L.), ginseng leaves (Panax ginseng C.A.Mey.) and water-alcohol extraction from this plant material the flavonoid content is studied because this group of biologically active substances has hypoglycemic effect. Qualitative reactions for different groups of flavonoids has confirmed their presence in blueberry shoots and ginseng leaves. In addition, qualitative analysis of water-alcohol extraction on their base using spectrophotometry has also showed presence of this group of biologically active substances. Along with that, quantitative content of flavonoids has been detected using differential spectrophotometry in blueberry shoots, ginseng leaves and water-alcohol extraction on their base.

Author(s):  
Shmygareva A. A. ◽  
Kurkin V. A. ◽  
Sankov A. N. ◽  
Nikandrova M. A.

The favorable climatic and geographic conditions for vegetation of medicinal plants of Orenburg region were described. Present information was confirmed by the results of qualitative and quantitative analysis of biologically active substances in different raw material, which grows on the territory of Orenburg region. The research’s results allow saying that the content of biologically active substances in raw material of studied plants corresponds to normative parameters, and in some cases exceeds them. The optimal extraction conditions for medical raw material were developed. Non-officinal plant was compared with officinal medical raw material and showed impressive results. The domestic raw material was compared with similar import one and proved its effectiveness in preclinical results. New medicinal drugs were developed on the bare of current results.


2018 ◽  
Vol 11 ◽  
pp. 00014
Author(s):  
Tatiana Fershalova ◽  
Elena Baikova ◽  
Alina Ushakova

The results of the experiments on optimization of library room air with the help of the biological method are presented. Living tropical plants, volatile biologically active substances of which possess distinct antimicrobic properties are used for air sanitation. Their influence on change of the qualitative and quantitative microorganism composition in the air is shown. After plant placement the total number of colonies forming units (CFU) decreases by 3 times, that of staphylococcus – by 4.5 times. Air humidity increases insignificantly reaching the low limit of the comfort range for a person.


2021 ◽  
pp. 56-63
Author(s):  
Iryna Fito ◽  
Nataliya Stadnytska

The aim. The aim of this study was to propose methods of standardization of Eucalyptus globulus leaves and Cetraria islandica slan by the main group of biologically active substances (BAS). This was done for further development of complex medicines based on Eucalyptus globulus leaves and Cetraria islandica slan. These studies provide an opportunity for further development and standardization of extracts from these plants, and the main aim of this study is the development and standardization of a complex medicine. Materials and methods. Thin layer chromatography was used to determine the presence of these markers. Quantitative values of these biologically active substances were determined by spectrophotometric method according to the relevant methods of the State Pharmacopoeia of Ukraine. Results. Markers for the certification of Cetraria islandica and Eucalyptus globulus have been proposed in this study. Chlorophylls and 1,8-cineole were chosen as a marker for the study of Eucalyptus globulus, and polysaccharides were chosen as a marker for Cetraria islandica. The qualitative and quantitative content of these markers in the studied objects was studied. Further research in this direction will be aimed at developing methods for control and standardization of water and alcohol extracts from these plants. Conclusions. Biologically active substances in medicinal plant raw materials were studied. It is proposed to study extracts and finished products from Eucalyptus globulus leaves and Cetraria islandica slan using these methods. The method of qualitative and quantitative determination of polysaccharides in Cetraria islandica slan is easy to apply and easily reproducible. It is important to be able to standardize Eucalyptus globulus leaves for essential oils and chlorophylls. The biologically active substances-markers found in Eucalyptus globulus leaves and Cetraria islandica slan will be used for the development of herbal medicines, the standardization of which will be carried out for the same compounds.


2019 ◽  
Vol 5 (10) ◽  
pp. 32-39 ◽  
Author(s):  
A. Sarukhanov ◽  
A. Morozova ◽  
N. Vasilyeva ◽  
D. Krylenkin

General information about biologically active substances flavonoids is concisely presented. The need for the quantitative determination of its content in products and the influence of product processing on flavonoid’s content is justified. Methods of qualitative and quantitative determination of flavonoids content in various products, medications and raw materials were studied. Based on this study the method of quantitative determination of flavonoid’s in samples of irradiated coriander is compiled and tested. Further ways to improve and adjust the compiled method for flavonoid’s content determination in irradiated samples via spectrophotometric analysis is considered.


Author(s):  
HRABOVSKA Olena ◽  
VITRIAK Oksana ◽  
AVRAMENKO Alina

Background. Taking into consideration the intense rhythm of life, the development of technologies for instant food products of increased nutritional value is urgent. Typically, food concentrates for desserts include fruit or fruit and berry fillings and require cooking to prepare ready-made meals. In this case, heat treatment of the mixture (boiling) negatively affects the thermolabile biologically active compounds. The aim of the work was to develop a recipe for instant food jelly concentrates of increased nutritional value based on encapsulated powdery hibiscus extract. Materials and methods. To encapsulate the aqueous-alcoholic extract of hibiscus and obtain a powdered semi-finished product, swelling potato starch was used. Rheological studies were carried out using a "REOTEST-2" rotational viscometer. Results. In order to enrich food concentrates of instant kissel with biologically active substances, we studied a method for extracting dry hibiscus flowers with aqueous-alcoholic solutions of various concentrations. It was found that the extractivity and the transfer of polyphenolic compounds, including anthocyanins, to the extract are improved when a water-alcohol mixture with an ethyl alcohol concentration of 30 % is used as an extractant. For the preparation of a powdery hibiscus extract, modified swelling starch was used, which has the property of swelling in cold water, which makes it possible not to subject the mixture to prolonged heat treatment for preparing a ready-made dish and to preserve the biologically active substances of the hibiscus extract. In addition, this starch is an effective solid carrier that plays a protective role for thermolabile vitamins and bioflavonoids. On the basis of experimental studies, formulations of instant jelly have been developed, which include fructose, powdered hibiscus extract, apple pectin and ascorbic acid. The study of the rheological properties of semi-finished products based on hibiscus extract, as well as ready-made jelly, testifies to the receipt of a finished dessert with a delicate creamy consistency. Conclusion. A method for obtaining a powdery encapsulated hibiscus extract based on swelling potato starch and a composition of a food jelly concentrate using this semi-finished product have been developed. It was found that for the extraction of raw materials it is better to use a water-alcohol mixture with an ethanol content of 30 %, since most of the anthocyanins are converted into the extract under these conditions. The developed semi-finished product of dry hibiscus extract can be used as a thickener, acidifier and colorant in recipes for food concentrates for instant dessert dishes. Due to the use of swelling starch, food concentrates of jelly do not need to be boiled, and the presence of vitamins, organic acids, flavonoids and especially anthocyanins in the hibiscus extract increases the nutritional value of the finished product.


2020 ◽  
Vol 9 (4) ◽  
pp. 72-78
Author(s):  
E. P. Rogozhnikova ◽  
P. G. Mizina ◽  
S. G. Mardanly

Introduction. This article considers the ways to optimize the motherwort tincture formulation while reducing the content of ethyl alcohol in the finished product. Qualitative and quantitative tincture test items depending on extraction method used and extractant concentration have been studied.Aim. To study the possibility of reducing the content of ethyl alcohol in motherwort tincture.Materials and methods. Comparison of quantitative and qualitative test items of tincture samples prepared by percolation, maceration, fractional maceration, modified fractional maceration is carried out. Quantitative comparative test items: dry residue, ethyl alcohol content, microbiological purity, quantitation, qualitative reactions – the presence of phenolic compounds, flavonoids in the samples (qualitative reactions, HPLC), iridoids (TLC) was used.Results and discussion. The developed method of modified fractional maceration consists in extracting herb materials with a more concentrated extractant (in the study, 80 %, 60 %, 40 % ethyl alcohol), then the subsequent extraction of herb materials with purified water. Comparison of the presence of different classes of biologically active substances in tinctures obtained by different technological methods and in infusions is carried out.Conclusion. It has been established that the most rational method is to obtain a tincture by modified fractional maceration method with ethyl alcohol content NLT 35 % in the finished product. The obtained results can be used in pharmaceutical industry for drug production by extractive methods, as well as for studies on technology optimization for obtaining extractive drugs from medicinal herb materials.


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