Dietary Intake and Serum Levels of Iron in Relation to Oxidative Stress in Breast Cancer Patients

Long non-coding RNAs play an important role in tumor growth, angiogenesis, and metastasis in several types of cancer. However, the clinical significance of using lncRNAs as biomarkers for breast cancer diagnosis and prognosis is still poorly investigated. In this study, we analyzed the serum expression levels of lncRNAs PVT1, HOTAIR, NEAT1, and MALAT1, and their associated proteins, PAI-1, and OPN, in breast cancer patients compared to fibroadenoma patients and healthy subjects. Using quantitative real-time PCR (qRT-PCR), we compared the serum expression levels of the four circulating lncRNAs in patients with breast cancer (n = 50), fibroadenoma (n = 25), and healthy controls (n = 25). The serum levels of PAI-1 and OPN were measured using ELISA. Receiveroperating-characteristic (ROC) analysis and multivariate logistic regression were used to evaluate the diagnostic value of the selected parameters. The serum levels of HOTAIR, PAI-1, and OPN were significantly higher in breast cancer patients compared to controls and fibroadenoma patients. The serum level of PVT1 was significantly higher in breast cancer patients than in the controls, while that of NEAT1 was significantly lower in breast cancer patients compared to controls and fibroadenoma patients. Both ROC and multivariate logistic regression analyses revealed that PAI-1 has the greatest power in discriminating breast cancer from the control, whereas HOTAIR, PAI-1, and OPN have the greatest power in discriminating breast cancer from fibroadenoma patients. In conclusion, our data suggest that the serum levels of PVT1, HOTAIR, NEAT1, PAI-1, and OPN could serve as promising diagnostic biomarkers for breast cancer.

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Differences in dietary intake during chemotherapy in breast cancer patients compared to women without cancer

Supportive Care in Cancer ◽

10.1007/s00520-017-3668-x ◽

2017 ◽

Vol 25 (8) ◽

pp. 2581-2591 ◽

Cited By ~ 26

Author(s):

Y. C. de Vries ◽

M. M. G. A. van den Berg ◽

J. H. M. de Vries ◽

S. Boesveldt ◽

J. Th. C. M. de Kruif ◽

...

Keyword(s):

Breast Cancer ◽

Dietary Intake ◽

Cancer Patients ◽

Breast Cancer Patients

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Exploring The Clinical Significance of Serum Angiopoietin-2 In Breast Cancer Patients

QJM ◽

10.1093/qjmed/hcab091.014 ◽

2021 ◽

Vol 114 (Supplement_1) ◽

Author(s):

Abeer I Abd Elmagid ◽

Hala Abdel Al ◽

Wessam El Sayed Saad ◽

Seham Kamal Mohamed

Keyword(s):

Breast Cancer ◽

Cancer Patients ◽

Serum Levels ◽

Tnm Staging ◽

Control Group ◽

Healthy Controls ◽

Breast Cancer Patients ◽

Female Patients ◽

Significant Difference ◽

Angiopoietin 2

Abstract Background Breast cancer is the most common cancer among women and one of the most important causes of death among them.Angiogenesis is an important step for primary tumor growth, invasiveness, and metastases. Angiopoietins are well-recognized endothelial growth factors that are involved in angiogenesis associated with tumors. Aim To explore the diagnostic significance of serum angiopoietin-2 (Ang-2) in breast cancer and to evaluate its prognostic efficacy through studying the degree of its association with the TNM staging of the disease. Patients and Methods This study was conducted on (35) Egyptian female patients who were diagnosed as breast cancer according to histopathological examination of breast biopsy (Group 1, Breast Cancer Patients) and (25) female patients with benign breast diseases (Group II, Pathological Control Patients), in addition to (20) age - matched apparently healthy, free mammogram, females serving as healthy controls (Group III, Healthy Controls). For all participants, measurement of serum Ang-2 was done using enzyme linked immunosorbent assay (ELISA) technique. Results A highly significant increased levels of Ang-2 was observed in breast cancer patients when compared to healthy control group (Z = 4.95, p < 0.01). However, no significant difference was observed in Ang-2 levels between breast cancer patients group and pathological control group (Z = 3.37, p > 0.05). No significant difference was detected in Ang-2 levels in relation to TNM stage and histological grade. No significant correlation was found between Ang-2 levels and serum levels of CA15-3, hormone receptors, HER2/new receptor status (p > 0.05, respectively). Conclusion This study revealed that Ang-2 serum levels were significantly increased in patient with breast cancer compared with healthy controls, indicating that high Ang-2 level is a promising non invasive biomarker for breast cancer diagnosis. However, no significant difference of Ang-2 levels was detected in relation of breast TNM staging in the population studied.

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Tissue and serum levels of phospho-hexo-isomerase and lactate dehydrogenase in breast cancer patients

Plastic & Reconstructive Surgery ◽

10.1097/00006534-199009000-00083 ◽

1990 ◽

Vol 86 (3) ◽

pp. 614

Author(s):

C. Maity ◽

Khoo Boo-Chai

Keyword(s):

Breast Cancer ◽

Lactate Dehydrogenase ◽

Cancer Patients ◽

Serum Levels ◽

Breast Cancer Patients

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Serum levels of oxidative stress marker malondialdehyde in breast cancer patients in relation to pathohistological factors, estrogen receptors, menopausal status, and age

Journal of Health Sciences ◽

10.17532/jhsci.2018.263 ◽

2018 ◽

Vol 8 (3) ◽

pp. 154-161

Author(s):

Jasmina Gubaljevic ◽

Nahida Srabović ◽

Adlija Jevrić-Čaušević ◽

Adaleta Softić ◽

Adi Rifatbegović ◽

...

Keyword(s):

Breast Cancer ◽

Lymph Node ◽

Lymph Nodes ◽

Cancer Patients ◽

Benign Breast Disease ◽

Menopausal Status ◽

Serum Levels ◽

Breast Disease ◽

Breast Cancer Patients ◽

Node Metastases

Introduction: The aim of this study was to determine the serum levels of malondialdehyde (MDA) in patients with invasive breast cancer in relation to its serum levels in patients with benign breast disease, and to investigate correlation between MDA serum levels with pathohistological prognostic factors (tumor size, lymph node involvement, and histologic grade [HG]), estrogen receptor (ER) status, and with breast cancer patient’s age and menopausal status. Methods: A total of 43 with well-documented invasive breast cancer were included in this study: 27 with positive axillary’s lymph nodes, and 16 with negative axillary’s lymph nodes, and 39 patients with findings of benign breast diseases. MDA determination in serum of breast cancer and benign breast disease patients was performed by the fluorimetric method, immunohistochemical staining was performed for ER, and routine pathohistological examination was conducted for pathohistological factors. Results: MDA serum levels in breast cancer patients were significantly higher than MDA serum levels in benign breast disease patients (p = 0.042). No statistically significant difference between MDA serum levels in breast cancer patients with and without lymph node metastases was found (p = 0.238). No statistically significant correlations between MDA serum levels and tumor size (p = 0.256), HG (p = 0.124), or number of positive lymph nodes (0.113) were found. A statistically significant correlation between serum MDA levels and ages of breast cancer patients with lymph node metastases was found (p = 0.006). Conclusion: Obtained results support the importance of MDA in the carcinogenesis of breast cancer. According to our findings, serum level of MDA could not be a useful prognostic factor in breast cancer.

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Roles of Superoxide dismutase(SOD) , Malondialdehyde (MDA), 8-iso-prostaglandinF2α ( 8-iso-PGF2α)as oxidative stress in development and progression of Brest cancer in Iraqi females patients

Al-Qadisiyah Journal Of Pure Science ◽

10.29350/jops.2020.25.1.1053 ◽

2020 ◽

Vol 25 (1) ◽

pp. 1-4

Author(s):

Ferdous Abbas Jabir ◽

Ahmed Sabah Shaker

Keyword(s):

Breast Cancer ◽

Oxidative Stress ◽

Cancer Patients ◽

Antioxidant Defenses ◽

Blood Levels ◽

P Value ◽

Oxygen Species ◽

Breast Cancer Patients ◽

Markers Of Oxidative Stress ◽

The Mean

Oxidative stress occurs as a result of disturbance in the balance between the production of reactive oxygen species (free radicals) and antioxidant defenses. Markers of oxidative stress were measured the markers of oxidative stress in breast cancer patients after diagnosis of breast cancer and compared these plasma blood levels controls This study was conducted to three markers of oxidative stress ;these are (SOD) enzyme ,malondialdehyde (MDA)and8-iso-prostaglandinF2α plasma of patients with breast cancer and compare with controls .In this study ; the mean MDA (ng/ml) levels for the breast cancer patients and the controls were55.91±3.31 and40.61±3.76 respectively, while the SOD (pg/ml) levels were1530.37±80.4 and1851.4 9±93.65 respectively and the 8-iso-PGF2α (ng/ml ) levels were 40.16±3.31 and 30.16±2.34 difference of the mean were statistically significant (p value <0.05).

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Independent Factors Predict Supranormal CA 15-3 Serum Levels in Advanced Breast Cancer Patients at First Disease Relapse

Tumor Biology ◽

10.1159/000050639 ◽

2001 ◽

Vol 22 (6) ◽

pp. 367-373 ◽

Cited By ~ 12

Author(s):

M. Tampellini ◽

A. Berruti ◽

G. Gorzegno ◽

R. Bitossi ◽

A. Bottini ◽

...

Keyword(s):

Breast Cancer ◽

Advanced Breast Cancer ◽

Cancer Patients ◽

Serum Levels ◽

Advanced Breast ◽

Disease Relapse ◽

Breast Cancer Patients

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CA 549 and SP2 in postoperative breast cancer patients. Comparison with CA 15.3, CEA and TPA

The International Journal of Biological Markers ◽

10.1177/172460089501000205 ◽

1995 ◽

Vol 10 (2) ◽

pp. 94-99 ◽

Cited By ~ 5

Author(s):

M. Torres ◽

C. Pacheco ◽

A. Valverde ◽

A.C. Rebollo ◽

A. Moral ◽

...

Keyword(s):

Breast Cancer ◽

Statistical Analysis ◽

Cancer Patients ◽

Blood Donors ◽

Serum Levels ◽

Breast Cancer Patients ◽

Benign Diseases ◽

Ca 15.3 ◽

Cancer Group ◽

Breast Cancer Group

The levels of CA 549 and SP2 were measured in 430 subjects: 100 healthy blood donors, 130 patients with benign diseases and 200 postoperative breast cancer patients. In the latter group, the serum levels of CA 15.3, CEA and TPA were also measured. The Kolmogorov-Smirnov, Mann Whitney and McNemar tests were used for statistical analysis. The upper normal limits were established on the basis of the values obtained in the healthy blood donors group, the benign diseases group and R.O.C. analysis of the breast cancer group. They were: CA 549 = 13 U/ml, SP2 = 14 U/ml, CA 15.3 = 35 U/ml, CEA = 5 ng/ml and TPA = 110 U/ml. The sensitivity, specificity and accuracy in the breast cancer group were, respectively: CA 549 = 78.1%, 97.1% and 88%; SP2 = 21.9%, 90.4% and 57.5%; CEA = 66.7%, 95.2% and 81.5%; CA 15.3 = 80.2%, 98.1% and 89.5%, and TPA = 73.9%, 78.8% and 76.5%. Statistical analysis showed significant differences only between CA 15.3, the marker which gave the best results, and SP2 (p<0.001). There were no significant differences with the association of two or three tumor markers.

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