Clinical study on black raspberry extract on the negative rate and effectiveness of patients with persistent human papilloma virus infection

To investigate the negative rate and effectiveness of black raspberry extract in clearing persistent infection with human papilloma virus ( HPV), t hree hundred patients are included in the randomized, double blind, placebo controlled human trial for multicenter indication . The distribution ratio of the test ed group versus the control group is 2: 1 and the subject patients were given a preparation in the vagina before bed every other day, each dose was 3 g containing black raspberry extract (tested group) or placebo (control group). SPSS 20.0 was used for statistical analysis. Differences between groups were analyzed by the Rank Sum method or Chi square method. The results showed that 191 subjects in the tested group completed the trial , of which 139 subjects ( 72.8% were identified to have an effective clear ance of HPV ; 95 subjects in the control group completed the trial , of which only 15 subjects ( 15.8% were identified to have effective clearance of HPV. The difference between the two groups was statistically significant (  2 = P 0.001 ). The HPV viral load of the tested group was decreased from 224.7 RLU/CO to 88.6 RLU/CO whereas HPV viral load of the control group was ascend ed from 218.4 RLU/CO to 266.1 RLU/CO Thus , the present clinical observation suggested that b lack raspberry extract may be a potential candidate in targeting persistent infection with HPV.

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Analysis of risk factors for persistent infection of asymptomatic women with high-risk human papilloma virus

Human Vaccines & Immunotherapeutics ◽

10.1080/21645515.2016.1239669 ◽

2017 ◽

Vol 13 (6) ◽

pp. 1404-1411 ◽

Cited By ~ 3

Author(s):

Nianmin Shi ◽

Qiang Lu ◽

Jiao Zhang ◽

Li Li ◽

Junnan Zhang ◽

...

Keyword(s):

Risk Factors ◽

High Risk ◽

Human Papilloma Virus ◽

Persistent Infection ◽

Papilloma Virus ◽

Asymptomatic Women

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Significance of survivin immunoreactivity and morphometric analysis of HPV-induced cervical dysplasia

Archives of Biological Sciences ◽

10.2298/abs1401429v ◽

2014 ◽

Vol 66 (1) ◽

pp. 429-436

Author(s):

Biserka Vukomanovic-Djurdjevic ◽

Gordana Basta-Jovanovic ◽

N. Baletic ◽

Milica Berisavac ◽

D. Nenadic ◽

...

Keyword(s):

High Risk ◽

Human Papilloma Virus ◽

Morphometric Analysis ◽

Cervical Dysplasia ◽

Epithelial Dysplasia ◽

Control Group ◽

High Grade ◽

Squamous Intraepithelial Lesion ◽

Papilloma Virus ◽

Intraepithelial Lesion

Genomic integration of high-risk human papilloma virus in the nucleus of cervical epithelial mucosal cells leads to epithelial dysplasia. The aim of this study was to determine the relevance of correlation between epithelial survivin expression and the degree of human papilloma virus (HPV)-induced cervical epithelial dysplasia, and to establish the significance of morphometric analysis of the nuclear area in the assessment of the degree of cervical dysplasia. This retrospective study included 99 women with primary, previously untreated lesions, and colposcopic findings indicating dysplasia, in whom a cytological test by Papanicolaou method was interpreted according to the Bethesda criteria as lowgrade squamous intraepithelial lesion (LSIL), high-grade squamous intraepithelial lesion (HSIL), and atypical squamous cells of undetermined significance (ASCUS). We performed human papilloma virus (HPV) typing by PCR for evidence of viruse types 16, 18, 31, 33. After biopsy of the cervical mucosa, we performed hematoxylin-eosin (H-E) and Periodic Acid Schiff (PAS) staining, and immunohistochemical and morphometric analysis of tissue samples. The control group consisted of 12 women without dysplasia and without a verified infection of cervical high-risk HPV. A high statistical correlation between the degree of dysplasia and expression of survivin was found in patients with different types of cervical dysplasia (p = 0.003). We observed a high statistical difference between the area of nuclei at different degrees of cervical dysplasias (p = 0.000). The high-grade cervical dysplasia had a more than 2-fold higher level of ranking in comparison to low-grade dysplasia, and a more than 10-fold higher ranking than the control group without cervical dysplasia.

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Associations among Serum Lipocalin-2 Concentration, Human Papilloma Virus, and Clinical Stage of Cervical Cancer

Medicina ◽

10.3390/medicina55060229 ◽

2019 ◽

Vol 55 (6) ◽

pp. 229 ◽

Cited By ~ 1

Author(s):

Agnė Vitkauskaitė ◽

Joana Celiešiūtė ◽

Saulius Paškauskas ◽

Erika Skrodenienė ◽

Rūta Jolanta Nadišauskienė ◽

...

Keyword(s):

Cervical Cancer ◽

Human Papilloma Virus ◽

Clinical Stage ◽

Stage Iv ◽

Hpv Infection ◽

Control Group ◽

Lipocalin 2 ◽

Papilloma Virus ◽

Tissue Biopsy ◽

Hpv Types

Background and objective: Lipocalin 2 (LCN2) has an oncogenic role in promoting tumorigenesis through enhancing tumor cell proliferation and the metastatic potential. The aim of our study was to determine whether serum LCN2 could serve as a diagnostic marker of cervical cancer (CC) and to evaluate the correlation between its serum concentration, the clinical stage of the cancer and Human Papilloma Virus HPV infections in women. Materials and methods: A total of 33 women with histologically proven cervical cancer (CC), 9 women with high- grade cervical intraepithelial neoplasia (HSIL) and 48 healthy women (NILM) were involved in the study. A concentration of LCN2 was assayed with the Magnetic LuminexR Assay multiplex kit. An HPV genotyping kit was used for the detection and differentiation of 15 high-risk (HR) HPV types in the liquid-based cytology medium (LBCM) and the tissue biopsy. Results: The majority (84.8%) of the women were infected by HPV16 in the CC group, and there was no woman with HPV16 in the control group (P < 0.01). Several types of HR HPV were found more often in the LBCM compared to in the tissue biopsy (P = 0.044). HPV16 was more frequently detected in the tissue biopsy than the LBCM (P < 0.05). The LCN2 level was higher in HPV-positive than in HPV-negative women (P = 0.029). The LCN2 concentration was significantly higher in women with stage IV than those with stage I CC (P = 0.021). Conclusions: Many HR HPV types, together with HPV16/18, can colonize the vagina and cervix, but often HPV16 alone penetrates into the tissue and causes CC. The serum LCN2 concentration was found to be associated not only with HR HPV infection, irrespective of the degree of cervical intraepithelial changes, but also with advanced clinical CC stage. LCN2 could be used to identify patients with advanced disease, who require a more aggressive treatment.

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Viral load of human papilloma virus 16 as a determinant for development of cervical carcinoma in situ: a nested case-control study

The Lancet ◽

10.1016/s0140-6736(00)02401-6 ◽

2000 ◽

Vol 355 (9222) ◽

pp. 2189-2193 ◽

Cited By ~ 234

Author(s):

Agnetha M Josefsson ◽

Patrik KE Magnusson ◽

Nathalie Ylitalo ◽

Per Sørensen ◽

Pernilla Qwarforth-Tubbin ◽

...

Keyword(s):

Viral Load ◽

Human Papilloma Virus ◽

Carcinoma In Situ ◽

Case Control Study ◽

Case Control ◽

Papilloma Virus ◽

Nested Case Control ◽

Human Papilloma Virus 16 ◽

Control Study

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Viral Load of Human Papilloma Virus 16 as a Determinant for Development of Cervical Carcinoma In Situ

Journal of Lower Genital Tract Disease ◽

10.1097/00128360-200101000-00016 ◽

2001 ◽

Vol 5 (1) ◽

pp. 58

Keyword(s):

Viral Load ◽

Human Papilloma Virus ◽

Cervical Carcinoma ◽

Carcinoma In Situ ◽

Papilloma Virus ◽

Human Papilloma Virus 16

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Significance of the Viral Load of High-risk Hpv in the Diagnosis and Prediction of Cervical Lesions: A Retrospective Study

10.21203/rs.3.rs-35312/v1 ◽

2020 ◽

Author(s):

Yang Liu ◽

Changjun Xu ◽

Jing Pan ◽

Chunyi Sun ◽

Honglin Zhou

Keyword(s):

Cervical Cancer ◽

Viral Load ◽

Retrospective Study ◽

High Risk ◽

High Viral Load ◽

Control Group ◽

Cervical Lesions ◽

Hpv Dna ◽

Cin Iii ◽

Hpv Viral Load

Abstract Background: The significance of HPV viral load in the detection of cervical lesions is still controversial. This study analyzed the correlation between the high-risk (HR)-HPV viral load and different cervical lesion degrees.Methods: This was a retrospective study of the patients who first visited the hospital between January 2015 and June 2018. Patients with positive HR-HPV were screening for cervical cancer. The HR-HPV DNA load was measured by the second generation hybrid capture (HC2) technology. The patients grouped as normal, CIN I, CIN II, CIN III, and cervical cancer. Multivariable logistic regression was performed to explore the association between HR-HPV DNA load and cervical lesions.Results: Finally, 265 patients were grouped as normal (n=125), CIN I (n=51), CIN II (n=23), CIN III (n=46), and cervical cancer (n=20). Among them, 139 (52.5%) had a low viral load, 90 (34.0) had a moderate viral load, and 36 (13.4%) had a high viral load. Taking the normal control group as a reference, a high viral load was an independent factor for CIN I (CIN I: OR=3.959, 95%CI: 1.300-12.059, P=0.015) CIN II (OR=6.211, 95%CI: 1.641-23.513, P=0.007), CIN III (OR=7.002, 95%CI: 2.308-21.244, P=0.001), and cervical cancer (OR=9.439, 95%CI: 2.394-37.22, P=0.001).Conclusion: Cervical lesions are closely related to HR-HPV infection. Higher HR-HPV viral load in cervical lesions was associated with a higher risk of high-grade cervical lesions.

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Assessment of Role of Human Papilloma Virus in Patients with Oral Squamous Cell Carcinoma

Journal of College of Medical Sciences-Nepal ◽

10.3126/jcmsn.v15i2.22707 ◽

2019 ◽

Vol 15 (2) ◽

pp. 125-127

Author(s):

Krishan Kumar Tyagi ◽

Megha Pradhan ◽

Rajbir Kaur Grewal ◽

Prabesh Sherchan ◽

KSA Pasha

Keyword(s):

Squamous Cell Carcinoma ◽

Oral Squamous Cell Carcinoma ◽

Human Papilloma Virus ◽

Cell Carcinoma ◽

Squamous Cell ◽

Control Group ◽

Papilloma Virus ◽

Mucosal Tissues ◽

Well Differentiated

Background: Head and neck malignancies include malignancies instigating in the oral cavity, the orophar-ynx, the hypopharynx and the larynx. We planned the present study to assess the role of human papilloma virus (HPV) in patients with oral squamous cell carcinoma (OSCC). Methods: The present study included assessment of role of human papilloma virus in patients with oral squamous cell carcinoma. A total of 50 histopathologic proven cases of well differentiated OSCC were included in the present study. A total of 50 healthy oral mucosal tissues (controls) were also included in the present study. The controls were obtained while surgical removing the impacted teeth. DNA was obtained from the tissue sections and underwent Polymerization chain reaction for identification of HPV 16/18. All the results were summarized in Microsoft excel sheet and were analyzed by SPSS software. Results: HPV was absent in all the cases of control group, whereas among the OSCC group, the HPV was detected in 46 percent of the cases. In the remaining 54 per-cent of the cases of the OSCC group, HPV was absent. Significant results were obtained while comparing the presence of HPV in between the OSCC group and the control group. Conclusions: HPV was present in sig-nificant number of OSCC cases. Therefore; HPV might play a contributing role in the etio-pathogenesis of OSCC.

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Demonstration of Epstein-Barr Virus DNA in Acetowhite Lesions of the Vulva

International Journal of STD & AIDS ◽

10.1177/095646249400500107 ◽

1994 ◽

Vol 5 (1) ◽

pp. 25-28 ◽

Cited By ~ 7

Author(s):

E Voog ◽

A Ricksten ◽

G B Löwhagen ◽

A Ternesten

Keyword(s):

Human Papilloma Virus ◽

Epstein Barr Virus ◽

Control Group ◽

Papilloma Virus ◽

Hpv Dna ◽

Barr Virus ◽

Oral Hairy Leukoplakia ◽

Hairy Leukoplakia ◽

Ebv Dna ◽

Epstein Barr

Acetowhite lesions in the vulva disclosing koilocytosis have been related to infection by human papilloma virus (HPV). Because of the clinical resemblance of these lesions to oral hairy leukoplakia (OHL), and EBV-manifestation, 23 women with acetowhite koilocytotic lesions in the vulva were examined. The PCR-technique was used to detect EBV DNA as well as HPV DNA in 17% of 23 patients examined. In a control group of 19 patients EBV DNA was detected in 11% and HPV DNA in 42% of cell samples from normal vulvar mucosa. EBV DNA has not previously been demonstrated in the vulvar mucosa, and this virus might be related to subclinical acetowhite lesions.

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