Copy number variation of ZNF280AY across 21 cattle breeds and its association with the reproductive traits of Holstein and Simmental bulls

Abstract. The insulin-like growth factor 1 receptor (IGF1R) plays a vital role in immunomodulation and muscle and bone growth. The copy number variation (CNV) is believed to the reason for many complex phenotypic variations. In this paper, we statistically analyzed the copy number and the expression profiling in different tissue types of the IGF1R gene using the 422 samples from four Chinese beef cattle breeds, and the mRNA of IGF1R was widely expressed in nine tissue types of adult cattle (heart, liver, kidney, muscle, fat, stomach, spleen, lung and testis). Results of CNV and growth traits indicated that the IGF1R CNV was significantly associated with body weight and body height of Jinnan (JN) cattle and was significantly associated with body height and hucklebone width of Qinchuan (QC) cattle, making IGF1R CNV a promising molecular marker to improve meat production in beef cattle breeding. Bioinformatics predictions show that the CNV region is highly similar to the human genome, and there are a large number of transcription factors, DNase I hypersensitive sites, and high levels of histone acetylation, suggesting that this region may play a role in transcriptional regulation, providing directions for further study of the role of bovine CNV and economic traits.

Download Full-text

Impact of ParentalBos taurusandBos indicusOrigins on Copy Number Variation in Traditional Chinese Cattle Breeds

Genome Biology and Evolution ◽

10.1093/gbe/evv151 ◽

2015 ◽

Vol 7 (8) ◽

pp. 2352-2361 ◽

Cited By ~ 10

Author(s):

Liangzhi Zhang ◽

Shangang Jia ◽

Martin Plath ◽

Yongzhen Huang ◽

Congjun Li ◽

...

Keyword(s):

Copy Number Variation ◽

Copy Number ◽

Cattle Breeds ◽

Chinese Cattle ◽

Number Variation

Download Full-text

The Association of the Copy Number Variation of the MLLT10 Gene with Growth Traits of Chinese Cattle

Animals ◽

10.3390/ani10020250 ◽

2020 ◽

Vol 10 (2) ◽

pp. 250

Author(s):

Peng Yang ◽

Zijing Zhang ◽

Jiawei Xu ◽

Kaixing Qu ◽

Shijie Lyv ◽

...

Keyword(s):

Correlation Analysis ◽

Copy Number Variation ◽

Copy Number ◽

High Throughput Screening ◽

Growth Traits ◽

Gene Copy ◽

Copy Number Variation Region ◽

Positive Effects ◽

Cattle Breeds ◽

Number Variation

Copy number variation is a part of genomic structural variation and has caused widespread concern. According to the results of high-throughput screening of the MLLT10 gene, we found that the copy number variation region of the MLLT10 gene was correlated with bovine growth traits. We aimed to detect the MLLT10 gene copy number variation and provide materials for the Chinese yellow cattle breed. In this study, the SPSS software was used to analyze the correlation among the copy number type of six different cattle breeds (i.e., Qinchuan, Xianan, Jiaxian, Yanbian, Sinan, Yunling) and the corresponding growth traits. The results showed the following: In Qinchuan cattle, the copy number duplication type was greater than the deletion and normal types; in Xianan cattle, the copy number duplication and normal types were less as compared with the deletion type; and in Yunling cattle, the frequency of the duplication type was dominant among the three types of copy number variants. The correlation analysis result showed that there is a significant correlation between the copy number variation (CNV) of the MLLT10 gene and the growth traits of three cattle breeds. Furthermore, correlation analysis showed that MLLT10 CNV had positive effects on growth traits such as hip width, rump length, hucklebone width, and cannon bone circumference (p < 0.05). This study provides a basis for the molecular-assisted marker breeding of cattle and contributes to the breeding of cattle.

Download Full-text