Different growth behaviors and metabolomic profiles in yogurts induced by multistrain probiotics of Lactobacillus casei Zhang and Bifidobacterium lactis V9 under different fermentation temperatures

Author(s):  
Jicheng Wang ◽  
Wei Zhao ◽  
Shuai Guo ◽  
Yaru Sun ◽  
Kai Yao ◽  
...  
2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 259-259
Author(s):  
Yuhui Zheng ◽  
Shengli Li

Abstract Bovine mastitis is one of the major diseases which directly affects the milk production performance and it causes huge economic losses in the dairy industry. Bacterial infection is the main risk factor of bovine mastitis and the antibiotic therapy is the primary choice to control the disease. However, persistence use of antibiotic increases the incidence of bacterial resistance and traces of antibiotic residues in animal products. Lactobacillus casei Zhang is one of the probiotics with multiple biological functions, which has certain bacteriostatic effect on pathogenic microorganism. The purpose of this study was to explore the effect of Lactobacillus casei Zhang (L. casei Zhang) on the prevention of E. coli-induced milk-blood barrier damage. Bovine mammary epithelial cells (BMECs) were used to establish a milk-blood model and Control group (PBS), E. coli group, and L. casei Zhang pretreatment plus E. coli group were set up respectively. The results showed that: L. casei Zhang could significantly reduce the increase of LDH release caused by E. coli treatment (P< 0.05). And it can also significantly reduce the decrease of transmembrane resistance of monolayer cells caused by E. coli treatment (P< 0.05). In addition, L. casei Zhang could significantly reduce the expression of tight junction proteins ZO-1, Claudin-1, Claudin-4 and Occludin (P < 0.05). In conclusion, L. casei Zhang could effectively improve the damage of the blood-milk barrier caused by E. coli and could protect BMECs during bacterial infection.


2020 ◽  
Vol 4 (11) ◽  
pp. 698-704
Author(s):  
E.S. Dzottsoeva ◽  
◽  
A.V. Gorelov ◽  
◽  

Acute respiratory infections (ARIs) in children are common conditions characterized by high morbidity. ARIs account for up to 75% of all cases of antibiotic use. Dysbiosis results in the impaired performance of vital functions, i.e., digestion, synthesis, colonization resistance, and the regulation of immune system. Probiotics are one of the effective tools to affect the composition of gut microbiota and to restore its metabolic activity. Probiotics, their effects on human organism, and their use in various diseases have received an enormous attention. Meanwhile, the use of antibiotics and the etiological diversity of ARIs account for the lack and irrationality of extensive preventive measures (as in flu) and raise the interest in non-specific prophylaxis. Lactobacillus rhamnosus GG (LGG) and Bifidobacterium animalis subsp. Lactis BB-12 are well-studied strains. Lactobacillus plantarum LP01 and LP02, Lactobacillus rhamnosus LR04 and LR05, and Bifidobacterium lactis BS01 used as monostrain probiotics are also of interest. Multistrain probiotics are gaining traction. However, these probiotics are understudied and require more attention. KEYWORDS: microbiome, monostrain probiotics, multistrain probiotics, acute respiratory infections, antibiotic-associated diarrhea, antibiotic-associated syndrome, Lactobacillus rhamnosus, Bifidobacterium lactis. FOR CITATION: Dzottsoeva E.S., Gorelov A.V. Monostrain and multistrain probiotics for respiratory diseases in children. Russian Medical Inquiry. 2020;4(11):698–704. DOI: 10.32364/2587-6821-2020-4-11-698-704.


2016 ◽  
Vol 99 (3) ◽  
pp. 1846-1852 ◽  
Author(s):  
Juan Zhang ◽  
Qian Liu ◽  
Wei Chen ◽  
Guocheng Du ◽  
Jian Chen

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