scholarly journals FREE RADICAL LIPID OXIDATION AS A LEADING MECHANISM IN DEVELPOING PERIODONTITIS

2020 ◽  
Vol 20 (1) ◽  
pp. 40-42
Author(s):  
G.Yu. Ostrovskaya ◽  
N.V. Rozkolupa ◽  
T.A. Petrova ◽  
E.G. Kolot ◽  
A.A. Kapustianskaya
Keyword(s):  
Free Radical ◽  
Periodontal Diseases ◽  
Dynamic Balance ◽  
Free Radical Oxidation ◽  
Occurrence Rate ◽  
Membrane Structures ◽  
Radical Oxidation ◽  
Local Factors ◽  
Periodontal Tissues ◽  
Skeletal Bone

Generalized periodontitis is a disease, which occurrence rate increases with age and manifests as a destructive inflammatory process based on the complex biochemical and pathophysiological changes. Active resorption of the alveolar ridge is influenced by both local factors and the overall body condition and systemic diseases. Development of generalized periodontitis is associated with loss of skeletal bone mass and characterized by predominance of resorption over osteosynthesis. Key moments in the pathogenesis of periodontal diseases are impairment of the dynamic balance of the prooxidate-antioxidant system that leads to the accumulation of reactive oxygen species and toxic metabolites. Along with the general mechanisms, local factors are involved into the pathogenesis of periodontitis. As a result of the "respiratory burst" of neutrophils, there is an effect of enzymatic lipid peroxidation burst with the following formation of superoxide anion radical and eicosanoids, damage to periodontal structures, immunological disorders. Generalized periodontitis is a polyetiological disease associated with the pathology of internal organs. The mechanisms of inflammatory and destructive periodontal lesions are mediated by genetic and local factors, chronic concomitant pathology, free radical aggression, and secondary immunodeficiency states. In the mechanism of local destruction of periodontal tissues, an important role is played by periodontopathogenic microflora, which triggers reactions accompanied by intensification of the processes of free radical oxidation of lipids and proteins of cell membrane structures.

scholarly journals CHARACTERISTICS OF CYTOKINE PROFILE OF ORAL FLUID IN PATIENTS WITH CHRONIC SIMPLE MARGINAL GINGIVITIS

2021 ◽  
Vol 11 (3) ◽  
pp. 75-79
Author(s):  
Adel Isaeva ◽  
Sergey Averyanov ◽  
Ilgiz Iskhakov ◽  
Shamil Galimov ◽  
Elmira Galimova ◽  
...  
Keyword(s):  
Free Radical ◽  
Oral Fluid ◽  
Periodontal Diseases ◽  
Free Radical Oxidation ◽  
Mechanisms Of Action ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Periodontal Tissues ◽  
Inflammatory Processes ◽  
Effective Drugs

Biomarkers of inflammation can be used as indicators for monitoring the treatment of periodontal diseases, as well as for finding the safest and most effective drugs with different mechanisms of action. The concentration of biomarkers in saliva was determined by enzyme immunoassay and chemiluminescent analysis. The obtained results reflect the degree of activity of inflammatory processes in periodontal tissues that occur in gingivitis and reveal an imbalance in the processes of free radical oxidation and antioxidant protection of the oral cavity.

scholarly journals Clinical effectiviness of dental NBF Gingival Gel in the treatment of patients with inflammatory periodontal diseases

2020 ◽  
Vol 102 (3) ◽  
pp. 32
Author(s):  
G. Biloklytska ◽  
◽  
O. Reshetniak ◽  
K. Gorgol ◽  
◽  
...  
Keyword(s):  
Mechanism Of Action ◽  
Periodontal Diseases ◽  
Free Radical Oxidation ◽  
Periodontal Therapy ◽  
Radical Oxidation ◽  
Periodontal Tissues ◽  
Antioxidant Mechanism ◽  
Biochemical Research ◽  
Periodontal Indices ◽  
Inflammatory Effect

Purpose. To examine the effectiveness of using local nano-gel NBF Gingival Gel at the stage of primary periodontal therapy of patients with chronic catarrhal gingivitis and generalized periodontitis based on the use of clinical and biochemical research methods. Methods. Appreciate the effectiveness of local nano-gel NBF Gingival Gel with the objective of periodontal indices, monitoring of patients and biochemical studies content in the oral fluid (OF) of indices of free radical oxidation (MDA) and antioxidant defense (glutathione reductase). Results. Usage of nano-gel NBF Gingival Gel at the stages of primary periodontal therapy for 80 patients with chronic catarrhal gingivitis (CCG) and generalized periodontitis (GP) I, I–II degree demonstrated good clinical anti-inflammatory effect, which based on its pronounced antioxidant and antimicrobial mechanisms of action. NBF Gingival Gel is not irritating of periodontal tissues and the mucous membrane of the oral cavity, has good organoleptic qualities. Conclusions: In addition to basic treatment in primary periodontal therapy of patients with inflammatory periodontal disease should be to use local drugs with directed pathogenetic mechanism of action, which include nano-gel NBF Gingival Gel with antioxidant mechanism of action.

OXIDATIVE STRESS IN EXPERIMENTAL PERIODONTITIS

2020 ◽  
pp. 67-72
Author(s):  
Zakhvatov A.N. ◽  
Hajdar D.A. ◽  
Tarasova T.V. ◽  
Avanesov A.M. ◽  
Samarina D.V. ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
Total Activity ◽  
The Body ◽  
Antioxidant Potential ◽  
Radical Oxidation ◽  
Periodontal Tissues ◽  
Markers Of Oxidative Stress ◽  
Experimental Periodontitis

Relevance. The study of bioradical processes in periodontitis in the dynamics of the disease and the establishment of the role of predictors of periodontal tissue damage will provide a deeper understanding of the links of pathogenesis and, on this basis, introduce new therapeutic and diagnostic technologies into practice. The purpose of the study: Assessment of the level of markers of oxidative stress in experimental periodontitis in the dynamics of the study. Materials and methods. The study was conducted on white non-linear rats, reproducing the model of periodontitis proposed by Shkolnaya K. D., Atrushkevich V. G. (Patent RU No. 2625295 of 12.07.2017). The total activity of free radical oxidation and the body's antioxidant potential were evaluated by biochemiluminescence, the intensity of lipoperoxidation processes, and the enzyme antioxidant potential. Results. A comprehensive analysis of the overall oxidative and antioxidant capacity of the body was carried out, during which it was revealed that progressive experimental periodontitis causes inhibition of the antioxidant potential and is characterized by a violation of the processes of neutralization of reactive oxygen species and the inability to inhibit the launched reactions of free radical oxidation, as evidenced by the dynamics of an increased level of markers of oxidative stress. Conclusion. Thus, in periodontitis, oxidative stress develops, which contributes to the progression of destructive processes of periodontal tissues, which substantiates the feasibility of using markers of the intensity of free radical oxidation as diagnostic and prognostic criteria for the course of the disease and the introduction of antioxidant-type drugs in complex therapy.

scholarly journals CLINICAL AND BIOCHEMICAL INDICATORS IN WHITE RATS IN THE TREATMENT OF EXPERIMENTAL GENERALIZED PARODONTITIS

2018 ◽  
pp. 9-13
Author(s):  
Yu.I. Silenko ◽  
M.V. Khrebor ◽  
Y.M. Silenko ◽  
O.A. Pisarenko
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
Modern Medicine ◽  
The Body ◽  
Radical Oxidation ◽  
Group Iii ◽  
Periodontal Tissues ◽  
White Rats ◽  
Group I

A number of scientists put forward and confirmed the assumption of the presence in the body of biological regulators, polypeptides, carrying out the transfer of information necessary for the functioning, development and interaction of cell populations. The problem of studying the mechanism of action and clinical application of polypeptides is key in modern medicine. The aim of the research is to examine the clinical parameters and the state of free radical oxidation in blood and periodontal tissues of white rats in the treatment of spontaneous generalized periodontitis with the use of polypeptides. Materials and methods of the research. Experiments were performed on 280 six-month-old of rats Wistar line of both sexes weighing 120-130 g. During the study, the animals were kept under vivarium conditions in individual cells, food and water were not limited. All animals were divided into the following groups: Group I – intact animals (70), Group II – animals with spontaneous periodontitis (70), Group III – animals with spontaneous periodontitis, treated with thymalin polypeptide preparation in a dose of 0.1 mg / kg i / m daily, for 10 days (70), Group ІV – animals with spontaneous paradontitis, treated with parodontylin polypeptide preparation at a dose of 1 mg / kg i / m, daily for 10 days (70). Blood for research in rats was taken against the background of hexenalum anesthesia with a syringe from the heart into a plastic syringe. Subsequently, it was mixed with sodium citrate (3.8% solution) in a ratio of 9:1 and centrifuged at 1500 rpm for 10 min. Periodontal tissues and jaws of animals were taken and examined immediately after euthanasia. For the study, the collection of periodontal tissues (gums with periosteum) was performed in the area of the upper and lower molars, since periodontitis often affects these tissues. Indicators of free radical oxidation were determined in blood and periodontal tissues. Resorption of bone tissue of the alveolar process was evaluated. Results and discussion. In the treatment of experimental spontaneous periodontitis using thymalin on the 7th day of observations, regression of symptoms of the disease in animals was observed. Hyperemia and edema, bleeding gums remitted. Whereas under the influence of thymalin there is a regression of dental symptoms by 3.8 times, under the influence of parodontylin – by 7.2 times. The condition of periodontal tissues in animals improved more significantly on the 20th day of observation, the scale assessment of dental status indicates that in animals, treated with thymalin, it is 1.1, and by parodontylin – 0.3 points per animal. In studying the processes of lipid peroxidation in periodontal tissues, their significant reduction in rats with spontaneous periodontitis after administering parodontylin has been established. We also observed an increase in the activity of antioxidant enzymes. Hence, the activity of SOD was twice as high, catalase – by 1.95 times. Reactions of lipid peroxidation had the same dynamics in the blood of animals after administering periodontal polypeptides and thymalin, as in periodontal tissues. We observed a significant decrease in the level of conjugated dienes, the concentration of the latter reaches the value of indices of intact animals. Similar results were obtained with regard to the level of TBA-active products, accumulation of MDA, spontaneous hemolysis of erythrocytes. Attention is drawn to the fact that parodontylin reduced lipid peroxygenation of blood to a greater extent than thymalin. We also observed increased activity of SOD, and, on the other hand, the concentration of ceruloplasmin decreased by 25.4%. Thus, administering periodontal polypeptides during spontaneous periodontitis leads to a decrease in the responses of lipid peroxidation in periodontal tissues and blood. To a greater extent this effect is characteristic of parodontylin in comparison with thymalin.

State of the enzyme link of antioxidant protection in boys with hypoandrogenism

2020 ◽  
Vol 40 (4) ◽  
pp. 32-36
Author(s):  
L. K. Parkhomenko ◽  
◽  
L. A. Strashok ◽  
S. I. Turchina ◽  
G. V. Kosovtsova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Free Radical ◽  
Glutathione Peroxidase ◽  
Free Radical Oxidation ◽  
Conjugated Dienes ◽  
Delayed Puberty ◽  
Control Group ◽  
Antioxidant Protection ◽  
Radical Oxidation

Recently, interest in the problem of free radical oxidation in biological membranes, which is directly related to both the normal functioning of cells and the occurrence, course and outcome of many pathological conditions, has increased again in clinical medicine. The aim was to determine the role and impact of antioxidant defense in boys with hypoandrogenism. The study involved 75 adolescents with hypoandrogenism aged 13–18 years, who underwent a complex of clinical and laboratory examinations. All patients were conducted complex of anthropometric research and determination of the degree of delayed puberty, laboratory and instrumental examination. Free radical oxidation was determined by the levels of malondialdehyde, conjugated dienes, carbonated proteins, superoxide dismutase and catalase in the serum, and restored glutathione and glutathione peroxidase in whole blood. Based on their determination, the coefficient of oxidative stress was calculated. Statistical processing of results was performed using parametric and nonparametric methods. The study of indicators of the free radical oxidation process found that adolescents with hypoandrogenism have multidirectional changes in the oxidation of proteins and lipids, namely: the level of conjugated dienes increases, the concentration of malondialdehyde remains at the level of the control group, and the level of carbonated proteins tends to decrease. As for the activity of antioxidant protection enzymes, a significant decrease in the level of glutathione peroxidase was detected, while the level of superoxide dismutase and catalase remained at the level of normative indicators. Oxidative stress accompanies and is one of the pathogenetic links in the formation or maintenance of the state of hypoandrogenism in boys. This requires the use of antioxidants, the complex of which must be selected individually.

Adaptogenic correction of free radical brain damage in subchronic exposure to sodium fl uoride

2020 ◽  
pp. 381-386
Author(s):  
A. G. Zhukova ◽  
L. G. Gorokhova ◽  
A. S. Kazitskaya ◽  
T. K. Yadykina ◽  
N. N. Mikhailova ◽  
...  
Keyword(s):  
Cerebral Cortex ◽  
Free Radical ◽  
Brain Tissue ◽  
Free Radical Oxidation ◽  
Radical Oxidation ◽  
Free Radical Processes ◽  
Subchronic Exposure ◽  
Antioxidant Defense Enzymes ◽  
Radical Processes ◽  
Complex Drug

Introduction. Fluorine compounds in small doses, but with prolonged exposure, cause various disorders in organs at the cellular and molecular levels. Activation of free-radical processes plays an important role in the damaging eff ect of fl uorides. Th erefore, one of the most eff ective ways to limit fl uorine-induced damage is to directly aff ect free-radical processes using herbal preparations with antioxidant properties.The aim of the study is to study the eff ect of a dihydroquercetin-based drug on the activity of free radical processes in brain tissue under subchronic exposure to sodium fl uoride (NaF).Materials and methods. Th e work was performed on white male laboratory rats weighing 200-250 g. Th e rats were divided into 3 groups: 1 — control; 2 — rats with chronic exposure to sodium fl uoride (NaF) for 9 weeks; 3 — rats receiving a NAF solution with simultaneous administration of a complex drug based on dihydroquercetin at a dose of 3 mg/kg in 1% starch gel for 3, 6 and 9 weeks. The activity of free radical oxidation and antioxidant defense enzymes — superoxide dismutase (SOD) and catalase-was determined in the cerebral cortex. Th e level of expression of hypoxia-induced transcription factor HIF — 1A and inducible forms of proteins HSP72 and HSP32 were determined in the cytosolic fraction of brain tissue.Results. In the early stages of subchronic fl uoride exposure (1-3 weeks), the expression of protective proteins HIF-1α, HSP72, HSP32 and catalase was shown in the rat cortex, as a result of which the activity of free-radical processes was maintained at the control level. An increase in the timing of fl uoride intake to 9 weeks led to a decrease in antioxidant protection and signifi cant activation of free radical oxidation in brain tissue. Daily administration of a complex drug with dihydroquercetin for 3, 6 and 9 weeks to rats with subchronic fl uoride exposure led to a decrease in the severity of pro- and antioxidant balance disorders in the cerebral cortex. At the same time, the greatest protective eff ect of dihydroquercetin with fl uoride exposure was manifested by the 9th week of its administration.Conclusions. When subchronic intake of fl uorides in the body, the drug based on dihydroquercetin has a neuroprotective eff ect, which is manifested by an increase in the activity of antioxidant enzymes of fr ee radical oxidation and catalase and the resistance of the cortex to induced fr ee radical oxidation.

Gender differences in biochemical measurement reflecting the state of free-radical oxidation and antioxidant protection among workers in metallurgical production

2019 ◽  
Vol 1 (10) ◽  
pp. 877-881
Author(s):  
I. A. Umnyagina ◽  
L. A. Strakhova ◽  
T. V. Blinova
Keyword(s):  
Oxidative Stress ◽  
Gender Differences ◽  
Free Radical ◽  
Biochemical Parameters ◽  
Free Radical Oxidation ◽  
The Body ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Metallurgical Production ◽  
Production Factors

Introduction. To date, age and sex differences have been established for many biochemical parameters. Gender differences in indicators for systems such as antioxidant, thiol-disulfide, oxidative stress and inflammation systems are absent or under study.The aim of the study was to identify gender differences in biochemical parameters reflecting the functioning of antioxidant systems of the body and free radical oxidation in workers of metallurgical production, in contact with harmful production factors.Materials and methods. The blood of men and women working at the metallurgical enterprise of the Nizhny Novgorod region (n=80) under the influence of a complex of physical and chemical production factors was studied. Total oxidative stress, total antioxidant capacity of serum, glutathione levels were studied by photometric biochemical methods. Levels of C-reactive protein and 8-hydroxy–2-deoxyguanosine were studied by ELISA.Results. The average amount of peroxides in the serum of women exceeded 1.6 times this figure in men. In the group of men, the content of 8-Ondg was higher by 26% (p=0.012), the level of GS-by 12% (p=0.019), the activity of SOD — by 1.5–2 times (p=0.0001), the level of CRP — by 2 times (p=0.008) compared to similar indicators in women.Conclusions. Studies of gender differences in workers under the influence of harmful production factors will allow more effective approach to the etiology, treatment and prognosis of production-related diseases. Indicators of oxidative stress and antioxidant protection can be indicators of the health of workers under the influence of harmful industrial factors and be important in the prevention of diseases associated with oxidative stress.

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