INTENSITY OF FREE RADICAL OXIDATION IN ORAL FLUID OF PATIENTS WITH PERIODONTAL DISEASES

2017 ◽  
Vol 19 (7) ◽  
pp. 121-123
Author(s):  
I.A. Sevostianov ◽  
◽  
A.G. Uvarova ◽  
O.V. Shvets ◽  
◽  
...  
Keyword(s):  
Free Radical ◽  
Oral Fluid ◽  
Periodontal Diseases ◽  
Free Radical Oxidation ◽  
Radical Oxidation

scholarly journals CHARACTERISTICS OF CYTOKINE PROFILE OF ORAL FLUID IN PATIENTS WITH CHRONIC SIMPLE MARGINAL GINGIVITIS

2021 ◽  
Vol 11 (3) ◽  
pp. 75-79
Author(s):  
Adel Isaeva ◽  
Sergey Averyanov ◽  
Ilgiz Iskhakov ◽  
Shamil Galimov ◽  
Elmira Galimova ◽  
...  
Keyword(s):  
Free Radical ◽  
Oral Fluid ◽  
Periodontal Diseases ◽  
Free Radical Oxidation ◽  
Mechanisms Of Action ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Periodontal Tissues ◽  
Inflammatory Processes ◽  
Effective Drugs

Biomarkers of inflammation can be used as indicators for monitoring the treatment of periodontal diseases, as well as for finding the safest and most effective drugs with different mechanisms of action. The concentration of biomarkers in saliva was determined by enzyme immunoassay and chemiluminescent analysis. The obtained results reflect the degree of activity of inflammatory processes in periodontal tissues that occur in gingivitis and reveal an imbalance in the processes of free radical oxidation and antioxidant protection of the oral cavity.

scholarly journals PECULIARITIES OF FREE RADICAL OXIDATION AND ANTIOXIDANT PROTECTION PARAMETERS OF THE ORAL FLUID IN CHILDREN WITH CHRONIC CATARRHAL GINGIVITIS WITH UNDERLYING DIABETES MELLITUS

2021 ◽  
Vol 74 (4) ◽  
pp. 887-890
Author(s):  
Liudmyla F. Kaskova ◽  
Valentyna A. Honcharenko ◽  
Oksana V. Klitynska
Keyword(s):  
Diabetes Mellitus ◽  
Free Radical ◽  
Oral Fluid ◽  
Lipid Peroxide ◽  
Free Radical Oxidation ◽  
Antioxidant Protection ◽  
State Of Health ◽  
General State ◽  
Peroxide Oxidation ◽  
Radical Oxidation

The aim: Objective of the research was to study peculiarities of free radical oxidation and antioxidant protection parameters of the oral fluid in children with chronic catarrhal gingivitis with underlying diabetes mellitus. Materials and methods: Oral fluid was examined in 105 children aged 12 including somatically healthy ones with intact periodontium and chronic catarrhal gingivitis, suffering from diabetes mellitus up to 5 years and more than 5 years with chronic catarrhal gingivitis. The parameters of lipid peroxide oxidation (level of diene conjugates, Malone dialdehyde) and antioxidant protection (activity of SOD and catalase, POM, whole protein; ceruloplasmin; НS-groups) of the oral fluid were determined. Results: The results obtained are indicative of available probable difference in the examined parameters among children depending on their general state of health and periodontal tissue condition. The best parameters were found among somatically healthy children with intact periodontium. They deteriorated in children with chronic catarrhal gingivitis available irrespective of their general state of health. They reached the most critical level in children with diabetes mellitus lasting longer than 5 years. Conclusions: The course of chronic catarrhal gingivitis in children, both somatically healthy and those suffering from diabetes mellitus, is associated with increased parameters of lipid peroxide oxidation and decreased enzymatic activity of the antioxidant protection system of the oral fluid. The most considerable changes are found in patients suffering from diabetes mellitus lasting more than 5 years.

scholarly journals FEATURES OF CLINICAL AND LABORATORY PARAMETERS AT THE PATIENS WITH RECURRENT ORAL AFTS AND CONDOMITANT ACID-DEPENDENT DISEASES OF THE GASTROINTESTINAL TRACT

2020 ◽  
Vol 16 (3) ◽  
pp. 23-29
Author(s):  
Irina Galimova ◽  
Rita Yunusova ◽  
Irina Usmanova ◽  
Larisa Gerasimova ◽  
Yigal Granot ◽  
...  
Keyword(s):  
Free Radical ◽  
Gastrointestinal Tract ◽  
Oral Cavity ◽  
Oral Hygiene ◽  
Oral Fluid ◽  
Free Radical Oxidation ◽  
The State ◽  
Radical Oxidation ◽  
Laboratory Parameters ◽  
Litmus Paper

Subject. Some features of the clinical picture, hygienic and microbiological state of the oral cavity, acidity and free-radical oxidation of the oral fluid in patients with recurrent aphthates on the background of acid-dependent diseases of the gastrointestinal tract are considered. The aim is to assess the relationship between clinical and laboratory parameters in patients with recurrent oral aphthae associated with acid-dependent diseases of the gastrointestinal tract. Methodology. A comprehensive clinical and laboratory examination of 58 young people with revealed recurrent aphthae of the oral cavity against the background of acid-dependent diseases of the gastrointestinal tract was carried out. The level of oral hygiene was assessed using the ONI-s index, the state of free-radical oxidation of the oral fluid - the "Chemilumimer-003" device, individual representatives of the normal flora were identified by inoculating the contents from the surface of aphthous elements on special differential diagnostic media of the HiCrome series. The acidity of the oral fluid was determined using a universal indicator litmus paper pH-1-14, short-term intragastric pHmetry was performed directly during fibrogastroduodenoscopy. Results. In 46.4 % of those examined, recurrent aphthae were found on the oral mucosa. In persons with chronic gastritis and duodenitis, the frequency of recurrences once a year was 37.9 %, 2-3 times a year ― 62.0, with the presence of chronic pancreatitis against the background of hyperacid gastric secretion ― 44.8 and 55.2, respectively. In patients with recurrent aphthae, a low level of oral hygiene, a violation of its microbiome, and a change in the acidity of the oral fluid were revealed. Conclusions. The presence of recurrent aphthae complicates individual oral hygiene, because accompanied by severe pain and paresthetic symptoms. This is manifested by an increase in the ONI-S index and a disturbance in the state of the normobiome in the form of an increase in the growth of resident microflora against the background of changes in the acidity of the oral fluid. Chemiluminescence indices correlate with the hygienic state of the oral cavity, qualitative changes in the normobiota and pH of the oral fluid in the studied clinical groups.

scholarly journals FREE RADICAL LIPID OXIDATION AS A LEADING MECHANISM IN DEVELPOING PERIODONTITIS

2020 ◽  
Vol 20 (1) ◽  
pp. 40-42
Author(s):  
G.Yu. Ostrovskaya ◽  
N.V. Rozkolupa ◽  
T.A. Petrova ◽  
E.G. Kolot ◽  
A.A. Kapustianskaya
Keyword(s):  
Free Radical ◽  
Periodontal Diseases ◽  
Dynamic Balance ◽  
Free Radical Oxidation ◽  
Occurrence Rate ◽  
Membrane Structures ◽  
Radical Oxidation ◽  
Local Factors ◽  
Periodontal Tissues ◽  
Skeletal Bone

Generalized periodontitis is a disease, which occurrence rate increases with age and manifests as a destructive inflammatory process based on the complex biochemical and pathophysiological changes. Active resorption of the alveolar ridge is influenced by both local factors and the overall body condition and systemic diseases. Development of generalized periodontitis is associated with loss of skeletal bone mass and characterized by predominance of resorption over osteosynthesis. Key moments in the pathogenesis of periodontal diseases are impairment of the dynamic balance of the prooxidate-antioxidant system that leads to the accumulation of reactive oxygen species and toxic metabolites. Along with the general mechanisms, local factors are involved into the pathogenesis of periodontitis. As a result of the "respiratory burst" of neutrophils, there is an effect of enzymatic lipid peroxidation burst with the following formation of superoxide anion radical and eicosanoids, damage to periodontal structures, immunological disorders. Generalized periodontitis is a polyetiological disease associated with the pathology of internal organs. The mechanisms of inflammatory and destructive periodontal lesions are mediated by genetic and local factors, chronic concomitant pathology, free radical aggression, and secondary immunodeficiency states. In the mechanism of local destruction of periodontal tissues, an important role is played by periodontopathogenic microflora, which triggers reactions accompanied by intensification of the processes of free radical oxidation of lipids and proteins of cell membrane structures.

scholarly journals Indices of Free Radical Oxidation in the Oral Fluid as Markers of Athletes’ Functional State

2017 ◽  
Vol 9 (3) ◽  
pp. 82
Author(s):  
K.N. Kontorshchikova ◽  
Y.R. Тikhomirova ◽  
A.N. Ovchinnikov ◽  
T.I. Kolegova ◽  
N.N. Churkina ◽  
...  
Keyword(s):  
Free Radical ◽  
Functional State ◽  
Oral Fluid ◽  
Free Radical Oxidation ◽  
Radical Oxidation

State of the enzyme link of antioxidant protection in boys with hypoandrogenism

2020 ◽  
Vol 40 (4) ◽  
pp. 32-36
Author(s):  
L. K. Parkhomenko ◽  
◽  
L. A. Strashok ◽  
S. I. Turchina ◽  
G. V. Kosovtsova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Free Radical ◽  
Glutathione Peroxidase ◽  
Free Radical Oxidation ◽  
Conjugated Dienes ◽  
Delayed Puberty ◽  
Control Group ◽  
Antioxidant Protection ◽  
Radical Oxidation

Recently, interest in the problem of free radical oxidation in biological membranes, which is directly related to both the normal functioning of cells and the occurrence, course and outcome of many pathological conditions, has increased again in clinical medicine. The aim was to determine the role and impact of antioxidant defense in boys with hypoandrogenism. The study involved 75 adolescents with hypoandrogenism aged 13–18 years, who underwent a complex of clinical and laboratory examinations. All patients were conducted complex of anthropometric research and determination of the degree of delayed puberty, laboratory and instrumental examination. Free radical oxidation was determined by the levels of malondialdehyde, conjugated dienes, carbonated proteins, superoxide dismutase and catalase in the serum, and restored glutathione and glutathione peroxidase in whole blood. Based on their determination, the coefficient of oxidative stress was calculated. Statistical processing of results was performed using parametric and nonparametric methods. The study of indicators of the free radical oxidation process found that adolescents with hypoandrogenism have multidirectional changes in the oxidation of proteins and lipids, namely: the level of conjugated dienes increases, the concentration of malondialdehyde remains at the level of the control group, and the level of carbonated proteins tends to decrease. As for the activity of antioxidant protection enzymes, a significant decrease in the level of glutathione peroxidase was detected, while the level of superoxide dismutase and catalase remained at the level of normative indicators. Oxidative stress accompanies and is one of the pathogenetic links in the formation or maintenance of the state of hypoandrogenism in boys. This requires the use of antioxidants, the complex of which must be selected individually.

Adaptogenic correction of free radical brain damage in subchronic exposure to sodium fl uoride

2020 ◽  
pp. 381-386
Author(s):  
A. G. Zhukova ◽  
L. G. Gorokhova ◽  
A. S. Kazitskaya ◽  
T. K. Yadykina ◽  
N. N. Mikhailova ◽  
...  
Keyword(s):  
Cerebral Cortex ◽  
Free Radical ◽  
Brain Tissue ◽  
Free Radical Oxidation ◽  
Radical Oxidation ◽  
Free Radical Processes ◽  
Subchronic Exposure ◽  
Antioxidant Defense Enzymes ◽  
Radical Processes ◽  
Complex Drug

Introduction. Fluorine compounds in small doses, but with prolonged exposure, cause various disorders in organs at the cellular and molecular levels. Activation of free-radical processes plays an important role in the damaging eff ect of fl uorides. Th erefore, one of the most eff ective ways to limit fl uorine-induced damage is to directly aff ect free-radical processes using herbal preparations with antioxidant properties.The aim of the study is to study the eff ect of a dihydroquercetin-based drug on the activity of free radical processes in brain tissue under subchronic exposure to sodium fl uoride (NaF).Materials and methods. Th e work was performed on white male laboratory rats weighing 200-250 g. Th e rats were divided into 3 groups: 1 — control; 2 — rats with chronic exposure to sodium fl uoride (NaF) for 9 weeks; 3 — rats receiving a NAF solution with simultaneous administration of a complex drug based on dihydroquercetin at a dose of 3 mg/kg in 1% starch gel for 3, 6 and 9 weeks. The activity of free radical oxidation and antioxidant defense enzymes — superoxide dismutase (SOD) and catalase-was determined in the cerebral cortex. Th e level of expression of hypoxia-induced transcription factor HIF — 1A and inducible forms of proteins HSP72 and HSP32 were determined in the cytosolic fraction of brain tissue.Results. In the early stages of subchronic fl uoride exposure (1-3 weeks), the expression of protective proteins HIF-1α, HSP72, HSP32 and catalase was shown in the rat cortex, as a result of which the activity of free-radical processes was maintained at the control level. An increase in the timing of fl uoride intake to 9 weeks led to a decrease in antioxidant protection and signifi cant activation of free radical oxidation in brain tissue. Daily administration of a complex drug with dihydroquercetin for 3, 6 and 9 weeks to rats with subchronic fl uoride exposure led to a decrease in the severity of pro- and antioxidant balance disorders in the cerebral cortex. At the same time, the greatest protective eff ect of dihydroquercetin with fl uoride exposure was manifested by the 9th week of its administration.Conclusions. When subchronic intake of fl uorides in the body, the drug based on dihydroquercetin has a neuroprotective eff ect, which is manifested by an increase in the activity of antioxidant enzymes of fr ee radical oxidation and catalase and the resistance of the cortex to induced fr ee radical oxidation.

Gender differences in biochemical measurement reflecting the state of free-radical oxidation and antioxidant protection among workers in metallurgical production

2019 ◽  
Vol 1 (10) ◽  
pp. 877-881
Author(s):  
I. A. Umnyagina ◽  
L. A. Strakhova ◽  
T. V. Blinova
Keyword(s):  
Oxidative Stress ◽  
Gender Differences ◽  
Free Radical ◽  
Biochemical Parameters ◽  
Free Radical Oxidation ◽  
The Body ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Metallurgical Production ◽  
Production Factors

Introduction. To date, age and sex differences have been established for many biochemical parameters. Gender differences in indicators for systems such as antioxidant, thiol-disulfide, oxidative stress and inflammation systems are absent or under study.The aim of the study was to identify gender differences in biochemical parameters reflecting the functioning of antioxidant systems of the body and free radical oxidation in workers of metallurgical production, in contact with harmful production factors.Materials and methods. The blood of men and women working at the metallurgical enterprise of the Nizhny Novgorod region (n=80) under the influence of a complex of physical and chemical production factors was studied. Total oxidative stress, total antioxidant capacity of serum, glutathione levels were studied by photometric biochemical methods. Levels of C-reactive protein and 8-hydroxy–2-deoxyguanosine were studied by ELISA.Results. The average amount of peroxides in the serum of women exceeded 1.6 times this figure in men. In the group of men, the content of 8-Ondg was higher by 26% (p=0.012), the level of GS-by 12% (p=0.019), the activity of SOD — by 1.5–2 times (p=0.0001), the level of CRP — by 2 times (p=0.008) compared to similar indicators in women.Conclusions. Studies of gender differences in workers under the influence of harmful production factors will allow more effective approach to the etiology, treatment and prognosis of production-related diseases. Indicators of oxidative stress and antioxidant protection can be indicators of the health of workers under the influence of harmful industrial factors and be important in the prevention of diseases associated with oxidative stress.

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