scholarly journals Anti-cancer effect of Gymnema sylvestre Leaf Extract against MG63, Human Osteosarcoma cell line - An in vitro analysis

2019 ◽  
Vol 11 (11) ◽  
pp. 18-24
Author(s):  
B . Packialakshmi ◽  
S. Raga Sowndriya
Keyword(s):  
Leaf Extract ◽  
Gymnema Sylvestre ◽  
Osteosarcoma Cell ◽  
Human Osteosarcoma Cell Line ◽  
Human Osteosarcoma ◽  
Anti Cancer ◽  
Human Osteosarcoma Cell ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals Formononetin Induces Apoptosis of Human Osteosarcoma Cell Line U2OS by Regulating the Expression of Bcl-2, Bax and MiR-375 In Vitro and In Vivo

2015 ◽  
Vol 37 (3) ◽  
pp. 933-939 ◽  
Author(s):  
Wei Hu ◽  
ZengMing Xiao
Keyword(s):  
Cell Line ◽  
Cell Apoptosis ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell ◽  
Human Osteosarcoma Cell Line ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cell ◽  
U2os Cells

Background/Aims: Phytoestrogens are known to prevent tumor progression by inhibiting proliferation and inducing apoptosis in cancer cells. Formononetin is one of the main components of red clover plants, and is considered as a typical phytoestrogen. This study investigates formononetin induction of apoptosis of human osteosarcoma cell line U2OS by regulating Bcl-2 and Bax expression in vitro and in vivo. Methods: U2OS cells were treated with different concentrations of formononetin and the proliferation of the cells was measured using an MTT assay. Cell apoptosis was examined by flow cytometry. The levels of miR-375, Bax and Bcl-2 protein expression in treated cells were determined by Western blot and RT-PCR. The antitumor activity of formononetin was also evaluated in vivo in nude mice bearing orthotopic tumor implants. Results: High concentrations of formononetin significantly suppress the proliferation of U2OS cells and induce cell apoptosis. Moreover, compared to control group the expression of Bcl-2 and miR-375 decreases with formononetin in the U2OS cells, while Bax increases. Conclusion: Formononetin has inhibitory effects on the proliferation of U2SO cells, both in vitro and in vivo. This antitumor effect is directly correlated with formononetin concentration.

In vitro evaluation of glass-glass ceramic thermoseed-induced hyperthermia on human osteosarcoma cell line

2011 ◽  
Vol 100A (1) ◽  
pp. 64-71 ◽  
Author(s):  
María Alcaide ◽  
Cecilia Ramírez-Santillán ◽  
María José Feito ◽  
María de la Concepción Matesanz ◽  
Eduardo Ruiz-Hernández ◽  
...  
Keyword(s):  
Cell Line ◽  
Glass Ceramic ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell ◽  
Human Osteosarcoma Cell Line ◽  
In Vitro Evaluation ◽  
Induced Hyperthermia ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cell

In Vitro Evaluation of the Cytotoxicity of Some New Titanium Alloys

2013 ◽  
Vol 587 ◽  
pp. 303-308 ◽  
Author(s):  
Andreea Carmen Bărbînţă ◽  
Kamel Earar ◽  
Carmen Iulia Crimu ◽  
Lucia Anişoara Drăgan ◽  
Corneliu Munteanu
Keyword(s):  
Titanium Alloys ◽  
Biomedical Applications ◽  
Colorimetric Assay ◽  
Osteosarcoma Cell ◽  
Human Osteosarcoma Cell Line ◽  
Cytotoxic Effects ◽  
Human Osteosarcoma ◽  
Mtt Colorimetric Assay ◽  
Human Osteosarcoma Cell

Titanium alloys are widely used in medical applications, due to their good mechanical properties, high corrosion resistance and biocompatibility. The aim of this paper was to investigate the cytotoxicity of novel titanium alloys: Ti21Nb6Zr15Ta, Ti25Nb10Zr8Ta, Ti17Nb5Zr5Al, Ti7Nb7Zr2Al with fibroblast-like cells derived from human osteosarcoma cell line (HOS). The results were compared with that of conventional biomedical alloys, like Ti6Al7Nb and Ti6Al4V. In vitro citotoxicity of titanium alloys was evaluated by fluorescence microscopy and MTT colorimetric assay. The results showed that the materials analyzed had no cytotoxic effects on HOS fibroblast-like cells, permitting their attachment and proliferation. Also the new titanium alloys present a higher cell viability than that of the conventional alloys. As a consequence, the TiNbZrTa and TiNbZrAl alloys can be considered as potential candidates for biomedical applications.

In vitro differentiation potential of a new human osteosarcoma cell line (HOS 58)

1998 ◽  
Vol 63 (2) ◽  
pp. 81-91 ◽  
Author(s):  
Heide Siggelkow ◽  
Christopher Niedhart ◽  
Wiebke Kurre ◽  
Angela Ihbe ◽  
Andreas Schulz ◽  
...  
Keyword(s):  
Cell Line ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell ◽  
In Vitro Differentiation ◽  
Human Osteosarcoma Cell Line ◽  
Differentiation Potential ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cell

scholarly journals Bioinspired-Metalloporphyrin Magnetic Nanocomposite as a Reusable Catalyst for Synthesis of Diastereomeric (−)-Isopulegol Epoxide: Anticancer Activity Against Human Osteosarcoma Cells (MG-63)

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 52 ◽  
Author(s):  
Lucas Dias ◽  
Ana Batista de Carvalho ◽  
Sara Pinto ◽  
Gilberto Aquino ◽  
Mário Calvete ◽  
...  
Keyword(s):  
Reusable Catalyst ◽  
Osteosarcoma Cell ◽  
Osteosarcoma Cells ◽  
Human Osteosarcoma Cell Line ◽  
Anticancer Effect ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells ◽  
Human Osteosarcoma Cell ◽  
Ic50 Values

In the present study, we developed a green epoxidation approach for the synthesis of the diastereomers of (−)-isopulegol benzyl ether epoxide using molecular oxygen as the oxidant and a hybrid manganese(III)-porphyrin magnetic reusable nanocomposite as the catalyst. High activity, selectivity, and stability were obtained, with up to four recycling cycles without the loss of activity and selectivity for epoxide. The anticancer effect of the newly synthesized isopulegol epoxide diastereomers was evaluated on a human osteosarcoma cell line (MG-63); both diastereomers showed similar in vitro potency. The measured IC50 values were significantly lower than those reported for other monoterpene analogues, rendering these epoxide isomers as promising anti-tumor agents against low prognosis osteosarcoma.

In Vitro Cytotoxicity of Binary Ti Alloys for Bone Implants

2009 ◽  
Vol 618-619 ◽  
pp. 295-298 ◽  
Author(s):  
Yun Cang Li ◽  
Cynthia Wong ◽  
Jian Yu Xiong ◽  
Peter D. Hodgson ◽  
Cui E Wen
Keyword(s):  
Weight Ratio ◽  
High Strength ◽  
In Vitro Cytotoxicity ◽  
Osteosarcoma Cell ◽  
Human Osteosarcoma Cell Line ◽  
Human Osteosarcoma ◽  
Ti Alloys ◽  
Human Osteosarcoma Cell ◽  
Cell Shapes

Interest in using titanium (Ti) alloys as load-bearing implant materials has increased due to their high strength to weight ratio, lower elastic modulus, and superior biocompatibility and enhanced corrosion resistance compared to conventional metals such as stainless steel and Co-Cr alloys. In the present study, the in vitro cytotoxicity of five binary titanium alloys, Ti15Ta, Ti15Nb, Ti15Zr, Ti15Sn and Ti15Mo, was assessed using human osteosarcoma cell line, SaOS-2 cells. The Cell proliferation and viability were determined, and cell adhesion and morphology on the surfaces of the binary Ti alloys after cell culture were observed by SEM. Results indicated that the Ti binary alloys of Ti15Ta, Ti15Nb and Ti15Zr exhibited the same level of excellent biocompatibility; Ti15Sn alloy exhibited a moderate biocompatibility while Ti15Mo alloy exhibited a moderate cytotoxicity. The SaOS-2 osteoblast-like cells had flattened and spread across the surfaces of the Ti15Ta, Ti15Nb, Ti15Zr and Ti15Sn groups; however, the cell shapes on the Ti15Mo alloy was shrinking and unhealthy. These results indicated that the Mo contents should be limited to a certain level in the design and development of new Ti alloys for implant material applications.

scholarly journals IN VIVO AND IN VITRO ANTITUMOR EFFECT OF NUTRIENT SYNERGY ON HUMAN OSTEOSARCOMA CELL LINE MNNG-HOS

2004 ◽  
Vol 12 (1/2) ◽  
pp. 135-145 ◽  
Author(s):  
M. Waheed Roomi ◽  
Vadim Ivanov ◽  
Tatiana Kalinovsky ◽  
Aleksandra Niedzwiecki ◽  
Matthias Rath
Keyword(s):  
Cell Line ◽  
Antitumor Effect ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell ◽  
Human Osteosarcoma Cell Line ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cell
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