Comparative Evaluation of Total and Free Sialic Acid in Saliva of Smokers and Tobacco Chewers to Determine Sialic Acid as A Salivary Biomarker for Oral Cancer

2020 ◽  
Vol 12 (01) ◽  
Keyword(s):  
Sialic Acid ◽  
Oral Cancer ◽  
Comparative Evaluation ◽  
Salivary Biomarker ◽  
Free Sialic Acid

scholarly journals Micronuclei and sialic acid as markers of genotoxic damage in tobacco-related oral lesions

2013 ◽  
Vol 3 (5) ◽  
pp. 379-385
Author(s):  
S Dahal ◽  
K Bozaz ◽  
N Srikant ◽  
K Reshma ◽  
N Agrawal
Keyword(s):  
Squamous Cell Carcinoma ◽  
Sialic Acid ◽  
Oral Cancer ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Serum Free ◽  
Genotoxic Damage ◽  
Oral Cells ◽  
Free Sialic Acid

Background: Carcinogens and co-carcinogens act in an additive, synergistic and antagonistic manner. Tobacco is the most common carcinogen related to oral cancer. The aim of this study was to assess the effect of tobacco on the oral epithelium by evaluating genotoxic damage to cells as quantitated by micronuclei and also the alterations to the cell membrane were correlated by the measurement of free sialic acid and protein-bound sialic acid in saliva and in serum. Materials and Methods: Blood samples collected from 15 untreated oral squamous cell carcinoma patients, 15 patients with oral precancerous conditions, 15 tobacco chewers without clinically evident lesion and 15 non-tobacco chewers. Serum and salivary sialic acid levels both in free and bound form were measured spectrophotometrically. Smear was taken from the same patient from the suspicious site and assessment of micronuclei was done by acridine orange method. Results: Serum free sialic acid and micronuclei assessment showed greater level of signifi cance (P=?0.05) between all other groups. This implies it’s potential use as an indicator of oral cancer. Predictability of occurrence of oral Cancer = 0.561+ 0.005× serum protein-bound sialic acid+ 0.244 serum free sialic acid + 0.71 M, where M=mean of micronuclei in 500 exfoliated oral cells. Predictability of occurrence of oral Premalignancy = 0.13+ 0.909× serum free sialic acid+ 0.045 M, where M=mean of micronuclei in 500 exfoliated oral cells. Conclusion: Micronuclei, serum and salivary sialic acid levels may be a good marker for prediction of premalignancy and oral squamous cell carcinoma transformation. Journal of Pathology of Nepal (2013) Vol. 3, No.1, Issue 5, 379-385 DOI: http://dx.doi.org/10.3126/jpn.v3i5.7862

Free Sialic Acid Storage Disease Mimicking Cerebral Palsy and Revealed by Blood Smear Examination

2011 ◽  
Vol 158 (1) ◽  
pp. 165-165.e1 ◽  
Author(s):  
François-Guillaume Debray ◽  
Caroline Lefebvre ◽  
Stéphanie Colinet ◽  
Karin Segers ◽  
René Stevens
Keyword(s):  
Cerebral Palsy ◽  
Sialic Acid ◽  
Blood Smear ◽  
Storage Disease ◽  
Smear Examination ◽  
Blood Smear Examination ◽  
Sialic Acid Storage Disease ◽  
Free Sialic Acid

scholarly journals Mini-Review Free Sialic Acid Storage Disorder: Progress and Promise

2021 ◽  
pp. 135896
Author(s):  
Marjan Huizing ◽  
Mary E. Hackbarth ◽  
David R. Adams ◽  
Melissa Wasserstein ◽  
Marc C. Patterson ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Storage Disorder ◽  
Free Sialic Acid

scholarly journals Inhibitory effect of free sialic acid on complement activation and its significance in hypocomplementemic glomerulonephritis

1999 ◽  
Vol 13 (4) ◽  
pp. 173-179 ◽  
Author(s):  
Takayuki Fujita ◽  
Atsushi Satomura ◽  
Mutsuko Hidaka ◽  
Isao Ohsawa ◽  
Morito Endo ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Complement Activation ◽  
Inhibitory Effect ◽  
Free Sialic Acid

Hypogammaglobulinemia and imaging features in a patient with infantile free sialic acid storage disease (ISSD) and a novel mutation in the SLC17A5 gene

2018 ◽  
Vol 31 (10) ◽  
pp. 1155-1159
Author(s):  
Tamara Žigman ◽  
Danijela Petković Ramadža ◽  
Mario Lušić ◽  
Marija Zekušić ◽  
Dorotea Ninković ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Storage Disease ◽  
Novel Mutation ◽  
Lysosomal Storage Diseases ◽  
Imaging Features ◽  
Lysosomal Storage ◽  
Biochemical Tests ◽  
Radiographic Findings ◽  
Sialic Acid Storage Disease ◽  
Free Sialic Acid

Abstract Background Infantile free sialic acid storage disease (ISSD) is a severe multisystemic disorder characterized by the accumulation of free sialic acid in lysosomes. Case presentation The patient presented prenatally with fetal ascites and large scrotal hernias, without pleural or pericardial effusion. During the infantile period, he was diagnosed with permanent isolated immunoglobulin G (IgG) hypogammaglobulinemia, which thus far has rarely been associated with ISSD. The analysis of the SLC17A5 gene revealed a novel homozygous 94 bp gene deletion. We further provide a detailed description of pre- and postnatal clinical and radiographic findings. Conclusions Fetal ascites could be the first sign of several lysosomal storage diseases (LSDs), including ISSD. The analysis of LSD gene panels is an effective approach to diagnosis in the case of non-specific symptoms and when specific biochemical tests are not easily available.

Correction of the Delayed Fibrin Aggregation of Fetal Fibrinogen by Partial Removal of Sialic Acid

1979 ◽  
Author(s):  
D.K. Galanakis ◽  
M.W. Mosesson
Keyword(s):  
Sialic Acid ◽  
Acid Content ◽  
Acid Value ◽  
Full Term ◽  
Thrombin Time ◽  
Sialic Acid Content ◽  
Vibrio Cholera ◽  
The Mean ◽  
Fraction I ◽  
Free Sialic Acid

Human umbilical oord fibrinogen characteristically displays delayed fibrin aggregation under conditions of relatively high ionic strength. This delay is greater in fibrinogen obtained from premature (p) (24–35 weeks gestation) infants, as compared with that from full term (FT) infants. We compared the sialic acid content of (fraction I-2) fetal (P and FT) with adult (A) fibrinogen, obtained from pooled plasma. The mean sialic acid μg/100mg protein) values were: P, 818 (±135 SD, range 621–1030); FT, 720 (±212, range 505–1280); A, 626 (±110, range 487-806). One P fibrinogen preparation (thrombin time 22.6 seconds) was partially desialated (resulting sialic acid value 490) by incubation with Vibrio cholera neuraminidase, dialyzed , and precipitated with ethanol to remove free sialic acid. The thrombin time of the resulting preparation was 15.4 (range 15.2–15.8), compared to 16.1 (range 15.5–16.4) for untreated A fibrinogen. The results suggest that the delayed fibrin aggregation of fetal fibrinogen is attributable to its relatively high sialic acid content. Moreover, the intermediate sialic acid (and thrombin time) values of FT (as compared to those of p) fibrinogen intimate the presence of a mixture of adult and fetal fibrinogen in full term cord blood.

Prenatal detection of Salla disease based upon increased free sialic acid in amniocytes

1987 ◽  
Vol 28 (2) ◽  
pp. 377-384 ◽  
Author(s):  
Martin Renlund ◽  
Pertti Aula ◽  
John M. Opitz ◽  
James F. Reynolds
Keyword(s):  
Sialic Acid ◽  
Prenatal Detection ◽  
Salla Disease ◽  
Free Sialic Acid

DIFFERENCES IN THE BIOLOGICAL AND IMMUNOLOGICAL ACTIVITIES OF HUMAN CHORIONIC GONADOTROPHIN AFTER THE REMOVAL OF SIALIC ACID BY ENZYMIC HYDROLYSIS

1967 ◽  
Vol 38 (4) ◽  
pp. 395-399 ◽  
Author(s):  
W. A. BARR ◽  
J. G. COLLEE
Keyword(s):  
Biological Activity ◽  
Sialic Acid ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Enzymic Hydrolysis ◽  
Immunological Activity ◽  
Parallel Reduction ◽  
Free Sialic Acid

SUMMARY Incubation of a partially purified preparation of human chorionic gonadotrophin (HCG) with neuraminidase resulted in release of free sialic acid from the substrate. The biological activity of the HCG was considerably reduced after incubation with the enzyme, but a parallel reduction in immunological activity was not observed.

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