Molecular Study of Fluoroquinolones Resistance Staphylococcus Aureus Isolated from Different Clinical Sources

2020 ◽  
Vol 12 (03) ◽  
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Study

scholarly journals Biofilm formation and molecular analysis of intercellular adhesion gene cluster (icaABCD) among Staphylococcus aureus strains isolated from children with adenoiditis

2021 ◽  
Author(s):  
Rasoul Ghaioumy ◽  
Fatemehalsadat Tabatabaeifar ◽  
Karamat Mozafarinia ◽  
Aliasghar Arabi Mianroodi ◽  
Elham Isaei ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Polymerase Chain Reaction ◽  
Gene Cluster ◽  
Biofilm Formation ◽  
Molecular Study ◽  
Adenoid Hypertrophy ◽  
Chain Reaction ◽  
Biofilm Production ◽  
Polymerase Chain ◽  
The Relationship

Background and Objectives: It is well known that Staphylococcus aureus biofilm plays an important role in adenoiditis and biofilm resistance frequently results in failure of therapy. The goal of this study was to evaluate the biofilm production of S. aureus isolates obtained from adenoid specimens and assess the relationship between biofilm formation ability and ica operon genes. Materials and Methods: A total of 112 adenoid samples were obtained from patients under 15 years old with adenoid hypertrophy. All S. aureus isolates were initially identified by standard microbiological tests and amplification of nuc by polymerase chain reaction (PCR) technique. Biofilm formation of S. aureus isolates was evaluated and icaADBC genes were detected by PCR technique. Results: There were 46 isolates (41%) identified as S. aureus. The ability to produce biofilm was detected among total S. aureus isolates. Molecular study of ica operon revealed that 2 (6.3%) and 19 (59.4%) isolates carried icaA and icaD, respec- tively. The prevalence of icaA + icaD was seen among 11 (34.4%) S. aureus isolates, while icaC and icaB were not detected. Conclusion: Our findings indicated that icaABCD operon are associated with biofilm formation in S. aureus isolates, how- ever the absence of these genes may not necessarily exclude this property.  

scholarly journals The Prevalence, Molecular Characterization and Antimicrobial Susceptibility of S. aureus Isolated from Impetigo Cases in Duhok, Iraq

2017 ◽  
Vol 11 (1) ◽  
pp. 22-29
Author(s):  
Marwan K. Al Zebary ◽  
Samira Y. Yousif ◽  
Mahde S. Assafi
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Susceptibility ◽  
Genomic Dna ◽  
Inverse Relationship ◽  
Molecular Study ◽  
Multi Drug Resistance ◽  
Opportunistic Pathogens ◽  
E Coli ◽  
The Common

Background:Staphylococcus aureusis one of the most important opportunistic pathogens. Impetigo is the common contagious bacterial infection of the skin caused byS. aureus.Method:Samples were taken from 204 patients with impetigo disease.S. aureusisolates were tested for their antimicrobial susceptibility. Genomic DNA ofS. aureuswas used to transformE. coliHB101 strain and expression capability ofS. aureusplasmids in transformedE. coliwas investigated. 68.62% (140/204) of the specimens were nonbullous impetigo and 31.38% (64/204) were bullous impetigo.S. aureusstrains were isolated from 41.66% (85/204) of impetigo cases (82.35% from nonbullous and 17.65% from the bullous impetigo). There was an inverse relationship between the incidence ofS. aureusisolated and age.Result:Three biotypes ofS. aureuswere identified based on their fermentation of different sugars. All isolates were resistant to penicillin and most isolates were resistant to ampicillin (95.3%), amoxicillin, (94.11%) and cephalexin (90.95%). Most isolates were sensitive against vancomycin and rifampicin (98.83%). 5.88% (5/85) ofS. aureusisolates were identified as MRSA. A maximum of 5 markers fromS. aureusisolates were capable to be expressed in transformedE. coliHB101 strains. The incidence of impetigo caused byS. aureusis comparable with reports from elsewhere.S. aureusisolates showed multidrug resistance against antibiotics.Conclusion:Plasmids ofS. aureusare capable to show its expression inE. coliHB101. Molecular study is needed to investigate the role of plasmids in different patterns of multi drug resistance.

scholarly journals Molecular study on methicillin-resistant Staphylococcus aureus strains isolated from dogs and associated personnel in Jordan

2015 ◽  
Vol 5 (11) ◽  
pp. 902-908 ◽  
Author(s):  
Yaser Hamadeh Tarazi ◽  
Ahmed Mahmoud Almajali ◽  
Mustafa Mohammad Kheer Ababneh ◽  
Humam Shawket Ahmed ◽  
Adnan Saleem Jaran
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Molecular Study ◽  
Methicillin Resistant

Cytochemical Studies of Cell Wall Biosynthesis in Staphylococcus Aureus

1972 ◽  
Vol 30 ◽  
pp. 328-329
Author(s):  
Masaatsu Koike ◽  
Koichi Nakashima ◽  
Kyoko Iida
Keyword(s):  
Staphylococcus Aureus ◽  
Periodate Oxidation ◽  
Early Time ◽  
The Other ◽  
Penicillin G ◽  
Cell Wall Biosynthesis ◽  
Septal Wall ◽  
Peptidoglycan Biosynthesis ◽  
Gram Positive Bacteria ◽  
Cross Linkage

Penicillin exerts the activity to inhibit the peptide cross linkage between each polysaccharide backbone at the final stage of wall-peptidoglycan biosynthesis of bacteria. Morphologically, alterations of the septal wall and mesosome in gram-positive bacteria, which were occurred in early time after treatment with penicillin, have been observed. In this experiment, these alterations were cytochemically investigated by means of silver-methenamine staining after periodate oxidation, which is applied for detection of localization of wall mucopolysaccharide.Staphylococcus aureus strain 209P treated with 100 u/ml of penicillin G was divided into two aliquotes. One was fixed by Kellenberger-Ryter's OSO4 fixative at 30, 60 and 120 min after addition of the antibiotic, dehydrated through alcohol series, and embedded in Epon 812 (Specimen A). The other was fixed by 21 glutaraldehyde, dehydrated through glycolmethacrylate series and embedded in glycolmethacrylate mixture, according to Bernhard's method (Specimen B).

Gold labeling of teichoic acid on adjacent surfaces of staphylococcus aureus

1990 ◽  
Vol 48 (3) ◽  
pp. 578-579
Author(s):  
Margaret Hukee
Keyword(s):  
Staphylococcus Aureus ◽  
Teichoic Acid ◽  
Protein Labeling ◽  
Minimal Amount ◽  
Section Thickness ◽  
Double Labeling ◽  
Parallel Surfaces ◽  
Supporting Membrane

Gold labeling of two antigens (double labeling) is often done on two section surfaces separated by section thickness. Whether labeling is done on both sides of the same section or on two parallel surfaces separated by section thickness (PSSST), comparable results are dependent on an equal number of epitopes being exposed at each surface. We propose a method to study protein labeling within the same field of proteins, by examining two directly adjacent surfaces that were split during sectioning. The number of labeling sites on adjacent surfaces (AS) were compared to sites on PSSST surfaces in individual bacteria.Since each bacteria needed to be recognizable in all three section surfaces, one-hole grids were used for labeling. One-hole grids require a supporting membrane and excessive handling during labeling often ruptures the membrane. To minimize handling, a labeling chamber was designed that is inexpensive, disposable, minimizes contamination, and uses a minimal amount of solution.

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