Analisis Variasi Komponen Fotoprotein dan Aldehid Pada Reaksi Bioluminesen Bakteri Luminesen Vibrio ordalii Asal Perairan Laut Papua

Eva Papilaya; Rita Sinaga

doi:10.31957/jbp.563

Analisis Variasi Komponen Fotoprotein dan Aldehid Pada Reaksi Bioluminesen Bakteri Luminesen Vibrio ordalii Asal Perairan Laut Papua

JURNAL BIOLOGI PAPUA ◽

10.31957/jbp.563 ◽

2018 ◽

Vol 2 (2) ◽

pp. 57-61

Author(s):

Eva Papilaya ◽

Rita Sinaga

Keyword(s):

Vibrio Ordalii

Riset pemancaran cahaya yang dilakukan pada bakteri luminesen semakin banyak dipelajari. Hal ini disebabkan bakteri ini sangat mudah untuk dikerjakan di laboratorium, dan mudah diperoleh. Salah satu bakteri yang telah diisolasi dari laut lokal Papua adalah spesies Vibrio ordalii, dan karakteristik biologi dan fisikanya telah dilakukan. Pemancaran cahaya yang dilakukan oleh bakteri ini terjadi melalui reaksi kimia, yang dikenal sebagai reaksi bioluminesen, yang melibatkan tiga komponen fotoprotein, yaitu lusiferase, lusiferin, dan oksigen. Pada riset ini dilakukan variasi terhadap dua komponen, lusiferase dan lusiferin, dan aldehid sebagai komponen pendukung dalam reaksi bioluminesen. Tujuan yang dicapai adalah untuk mengetahui pengaruh variasi kedua komponen terhadap pemancaran cahaya bakteri. Metode yang digunakan adalah metode assay ditionit dan spektrometri dengan menggunakan spektrofotometer uv-vis. Hasil penelitian menunjukkan bahwa aldehid dengan konsentrasi di atas 0,01% tidak meningkatkan luminesen.Key words: bioluminescent, Vibrio ordalii, fotoprotein, aldehid, lusiferin, lusiferase.

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The Fish Pathogen Vibrio ordalii Under Iron Deprivation Produces the Siderophore Piscibactin

Microorganisms ◽

10.3390/microorganisms7090313 ◽

2019 ◽

Vol 7 (9) ◽

pp. 313 ◽

Cited By ~ 3

Author(s):

Pamela Ruiz ◽

Miguel Balado ◽

Juan Carlos Fuentes-Monteverde ◽

Alicia E. Toranzo ◽

Jaime Rodríguez ◽

...

Keyword(s):

Gene Cluster ◽

Iron Uptake ◽

Peptide Mass Fingerprinting ◽

Iron Limitation ◽

Fish Pathogen ◽

Fish Pathogens ◽

Iron Deprivation ◽

Peptide Mass ◽

Photobacterium Damselae ◽

Vibrio Ordalii

Vibrio ordalii is the causative agent of vibriosis, mainly in salmonid fishes, and its virulence mechanisms are still not completely understood. In previous works we demonstrated that V. ordalii possess several iron uptake mechanisms based on heme utilization and siderophore production. The aim of the present work was to confirm the production and utilization of piscibactin as a siderophore by V. ordalii. Using genetic analysis, identification by peptide mass fingerprinting (PMF) of iron-regulated membrane proteins and chemical identification by LC-HRMS, we were able to clearly demonstrate that V. ordalii produces piscibactin under iron limitation. The synthesis and transport of this siderophore is encoded by a chromosomal gene cluster homologous to another one described in V. anguillarum, which also encodes the synthesis of piscibactin. Using β-galactosidase assays we were able to show that two potential promoters regulated by iron control the transcription of this gene cluster in V. ordalii. Moreover, biosynthetic and transport proteins corresponding to piscibactin synthesis and uptake could be identified in membrane fractions of V. ordalii cells grown under iron limitation. The synthesis of piscibactin was previously reported in other fish pathogens like Photobacterium damselae subsp. piscicida and V. anguillarum, which highlights the importance of this siderophore as a key virulence factor in Vibrionaceae bacteria infecting poikilothermic animals.

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A probiotic strain of Vibrio alginolyticus effective in reducing diseases caused by Aeromonas salmonicida, Vibrio anguillarum and Vibrio ordalii

Journal of Fish Diseases ◽

10.1111/j.1365-2761.1995.tb01271.x ◽

1995 ◽

Vol 18 (1) ◽

pp. 93-96 ◽

Cited By ~ 224

Author(s):

B. AUSTIN ◽

L. F. STUCKEY ◽

P. A. W. ROBERTSON ◽

I. EFFENDI ◽

D. R. W. GRIFFITH

Keyword(s):

Vibrio Anguillarum ◽

Probiotic Strain ◽

Aeromonas Salmonicida ◽

Vibrio Alginolyticus ◽

Vibrio Ordalii

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Localization of the Replication Region of the pMJ101 Plasmid from Vibrio ordalii

Plasmid ◽

10.1006/plas.1994.1026 ◽

1994 ◽

Vol 31 (3) ◽

pp. 242-250 ◽

Cited By ~ 8

Author(s):

Carla Bidinost ◽

Jorge H. Crosa ◽

Luis A. Actis

Keyword(s):

Replication Region ◽

Vibrio Ordalii

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Cell-surface properties of Vibrio ordalii strains isolated from Atlantic salmon Salmo salar in Chilean farms

Diseases of Aquatic Organisms ◽

10.3354/dao02820 ◽

2015 ◽

Vol 113 (1) ◽

pp. 9-23 ◽

Cited By ~ 11

Author(s):

P Ruiz ◽

M Poblete ◽

AJ Yáñez ◽

R Irgang ◽

AE Toranzo ◽

...

Keyword(s):

Atlantic Salmon ◽

Cell Surface ◽

Surface Properties ◽

Salmo Salar ◽

Cell Surface Properties ◽

Atlantic Salmon Salmo Salar ◽

Vibrio Ordalii

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Structural investigation of the lipopolysaccharide core isolated from a virulent strain of Vibrio ordalii

Canadian Journal of Biochemistry and Cell Biology ◽

10.1139/o85-150 ◽

1985 ◽

Vol 63 (12) ◽

pp. 1199-1205 ◽

Cited By ~ 10

Author(s):

Joseph H. Banoub ◽

Howard J. Hodder

Keyword(s):

Nuclear Magnetic Resonance ◽

Nitrous Acid ◽

Structural Investigation ◽

Chromium Trioxide ◽

Virulent Strain ◽

Partial Hydrolysis ◽

Methylation Analysis ◽

Core Oligosaccharide ◽

The Core ◽

Vibrio Ordalii

The structure of the core oligosaccharide of Vibrio ordalii has been investigated. The studies involved the use of nuclear magnetic resonance, methylation analysis, partial hydrolysis with hydrochloric acid, nitrous acid deamination, partial hydrolysis with sulfuric acid, Smith degradation, and oxidation with chromium trioxide. As a result of these studies the following structure is proposed.[Formula: see text]

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Numerical taxonomy of Vibrionaceae from Newfoundland coastal waters

Canadian Journal of Microbiology ◽

10.1139/m94-058 ◽

1994 ◽

Vol 40 (5) ◽

pp. 355-361 ◽

Cited By ~ 10

Author(s):

Jennifer Martin-Kearley ◽

John A. Gow

Keyword(s):

Numerical Analysis ◽

Coastal Waters ◽

Numerical Taxonomy ◽

Incubation Temperature ◽

Placopecten Magellanicus ◽

Arginine Dihydrolase ◽

Alaria Esculenta ◽

The Family ◽

Vibrio Ordalii ◽

Positive Results

Numerical analysis was used to characterize 111 Vibrionaceae strains. These included 31 reference cultures belonging to the genera Aeromonas, Listonella, and Vibrio and 80 strains isolated from the seasonally cold coastal waters of Newfoundland. The sources of the regional strains were the brown alga Alaria esculenta and the giant scallop Placopecten magellanicus. Most of the regional strains grew at temperatures associated with psychrotrophs, while a small proportion may have been psychrophilic. All of the regional strains grew at 4 °C. A routine incubation temperature of 20 °C was chosen and in tests for utilization of organic components as sole sources of carbon and energy the strains were incubated for 3 weeks rather than the more common 6-day period. The treatment of weak positive results as weak positive, positive, or negative was investigated and it was decided that the general conclusions reached in the study would not be significantly altered by the interpretation of weak positive results. Using numerical analysis it was shown that most of the strains clustered according to source. Most reference cultures were more closely related to each other than they were to the regional strains. Some strains were phenotypically similar to Vibrio splendidus biovar I, which is arginine dihydrolase positive. Although there were differences, some strains were similar to the fish pathogen Vibrio ordalii, which is negative for arginine dihydrolase. Both species are reported to grow at 4 °C. It was shown that most of the regional Vibrionaceae strains studied were different from previously described species belonging to the family Vibrionaceae.Key words: numerical taxonomy, Vibrionaceae, Vibrio, marine bacteria, psychrotroph.

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