Numerical taxonomy of Vibrionaceae from Newfoundland coastal waters

1994 ◽  
Vol 40 (5) ◽  
pp. 355-361 ◽  
Author(s):  
Jennifer Martin-Kearley ◽  
John A. Gow
Keyword(s):  
Numerical Analysis ◽  
Coastal Waters ◽  
Numerical Taxonomy ◽  
Incubation Temperature ◽  
Placopecten Magellanicus ◽  
Arginine Dihydrolase ◽  
Alaria Esculenta ◽  
The Family ◽  
Vibrio Ordalii ◽  
Positive Results

Numerical analysis was used to characterize 111 Vibrionaceae strains. These included 31 reference cultures belonging to the genera Aeromonas, Listonella, and Vibrio and 80 strains isolated from the seasonally cold coastal waters of Newfoundland. The sources of the regional strains were the brown alga Alaria esculenta and the giant scallop Placopecten magellanicus. Most of the regional strains grew at temperatures associated with psychrotrophs, while a small proportion may have been psychrophilic. All of the regional strains grew at 4 °C. A routine incubation temperature of 20 °C was chosen and in tests for utilization of organic components as sole sources of carbon and energy the strains were incubated for 3 weeks rather than the more common 6-day period. The treatment of weak positive results as weak positive, positive, or negative was investigated and it was decided that the general conclusions reached in the study would not be significantly altered by the interpretation of weak positive results. Using numerical analysis it was shown that most of the strains clustered according to source. Most reference cultures were more closely related to each other than they were to the regional strains. Some strains were phenotypically similar to Vibrio splendidus biovar I, which is arginine dihydrolase positive. Although there were differences, some strains were similar to the fish pathogen Vibrio ordalii, which is negative for arginine dihydrolase. Both species are reported to grow at 4 °C. It was shown that most of the regional Vibrionaceae strains studied were different from previously described species belonging to the family Vibrionaceae.Key words: numerical taxonomy, Vibrionaceae, Vibrio, marine bacteria, psychrotroph.

Numerical analysis and the application of random amplified polymorphic DNA polymerase chain reaction to the differentiation of Vibrio strains from a seasonally cold ocean

1994 ◽  
Vol 40 (6) ◽  
pp. 446-455 ◽  
Author(s):  
Jennifer Martin-Kearley ◽  
John A. Gow ◽  
Marc Péloquin ◽  
Charles W. Greer
Keyword(s):  
Polymerase Chain Reaction ◽  
Numerical Analysis ◽  
Dna Polymerase ◽  
Random Amplified Polymorphic Dna ◽  
Placopecten Magellanicus ◽  
Chain Reaction ◽  
Phenotypic Properties ◽  
Arginine Dihydrolase ◽  
Rapd Pcr ◽  
Polymerase Chain

Eighty regional strains of Vibrio isolated from the seasonally cold waters of coastal Newfoundland, and a number of Vibrio reference cultures, were studied. The regional strains had been isolated from the brown macroalga Alaria esculenta and the giant scallop Placopecten magellanicus and were known to grow at 4 °C. The strains were grouped according to their arginine-dihydrolase reactions and examined by numerical analysis. According to phenotypic properties the arginine-dihydrolase positive strains closely resembled Vibrio splendidus biovar I. Most clusters of the arginine-dihydrolase negative strains appeared to be unique but the closest phenotypic resemblance among some strains was with Vibrio ordalii. Some strains were examined using the random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) technique for fingerprinting and it was shown that the regional strains were significantly different from either V. splendidus biovar I or V. ordalii. Generally, the strains from seaweed clustered separately from those that were from scallops. Strains in some clusters, especially those from the seaweed, were able to utilize most of the compounds that were tested as sole sources of carbon and energy.Key words: numerical taxonomy, marine bacteria, Vibrio, psychrotrophs, RAPD-PCR.

scholarly journals Isolation and Phylogenetic Analysis of Novel Viruses Infecting the Phytoplankton Phaeocystis globosa (Prymnesiophyceae)

2004 ◽  
Vol 70 (6) ◽  
pp. 3700-3705 ◽  
Author(s):  
C. P. D. Brussaard ◽  
S. M. Short ◽  
C. M. Frederickson ◽  
C. A. Suttle
Keyword(s):  
Phylogenetic Analysis ◽  
Dna Polymerase ◽  
Coastal Waters ◽  
Common Ancestor ◽  
Genetic Relatedness ◽  
Amino Acid Sequences ◽  
Polymerase Gene ◽  
Phaeocystis Globosa ◽  
The Family ◽  
Algal Host

ABSTRACT Viruses infecting the harmful bloom-causing alga Phaeocystis globosa (Prymnesiophyceae) were readily isolated from Dutch coastal waters (southern North Sea) in 2000 and 2001. Our data show a large increase in the abundance of putative P. globosa viruses during blooms of P. globosa, suggesting that viruses are an important source of mortality for this alga. In order to examine genetic relatedness among viruses infecting P. globosa and other phytoplankton, DNA polymerase gene (pol) fragments were amplified and the inferred amino acid sequences were phylogenetically analyzed. The results demonstrated that viruses infecting P. globosa formed a closely related monophyletic group within the family Phycodnaviridae, with at least 96.9% similarity to each other. The sequences grouped most closely with others from viruses that infect the prymnesiophyte algae Chrysochromulina brevifilum and Chrysochromulina strobilus. Whether the P. globosa viruses belong to the genus Prymnesiovirus or form a separate group needs further study. Our data suggest that, like their phytoplankton hosts, the Chrysochromulina and Phaeocystis viruses share a common ancestor and that these prymnesioviruses and their algal host have coevolved.

Caligids-potential pathogens for marine finfish aquaculture in Mexico?

2017 ◽  
Vol 44 (3) ◽  
pp. 433-441
Author(s):  
Francisco Neptalí Morales-Serna ◽  
Juan Manuel Martínez-Brown ◽  
Rosa Maria Medina-Guerrero ◽  
Emma Josefina Fajer-Ávila
Keyword(s):  
Coastal Waters ◽  
Host Location ◽  
Current Knowledge ◽  
Control Strategies ◽  
Sea Lice ◽  
Ecological Studies ◽  
Parasitic Copepods ◽  
The Family ◽  
And Control ◽  
Finfish Aquaculture

Parasitic copepods of the family Caligidae, the so-called sea lice, may be deleterious to marine or brackish finfish aquaculture. To date, biological and ecological studies of sea lice have been mostly restricted to species from cold or temperate regions. In Mexico there are some records of sea lice species on marine fishes; however, the research regarding their biology and ecology has been scarce. It is possible that a high biodiversity of sea lice is distributed in coastal waters of Mexico; therefore, their significance as pathogenic parasites should increase. The purpose of this review is to outline the current knowledge of the life cycle, host location, ecology, effect on fish health, and control strategies of sea lice in order to establish supportive basis for natural resource management and control of parasites and diseases of marine fish cultured in Mexico.

Distribution of Diplolaimella and Diplolaimelloides species from Patagonian lagoons and coastal waters (Nematoda: Monhysteridae), Chubut and Santa Cruz provinces (Argentina)

2009 ◽  
Vol 89 (4) ◽  
pp. 711-718 ◽  
Author(s):  
Catalina T. Pastor de Ward ◽  
Virginia Lo Russo
Keyword(s):  
New Species ◽  
Coastal Waters ◽  
East Coast ◽  
Santa Cruz ◽  
Coastal Salt Marsh ◽  
Salty Water ◽  
The Family ◽  
Two New Species ◽  
New Localities ◽  
First Time

This paper focuses on Diplolaimella and Diplolaimelloides two very cosmopolitan genera inhabiting lotic freshwater to salty water inland, groundwater and coastal waters systems, providing a review of their taxonomy with new keys, and their distribution on the east coast of Patagonia and inland Patagonia. Two new species belonging to the genus Diplolaimelloides of the family Monhysteridae are described from Patagonian salty lagoons and coastal areas, and the Chubut and Santa Cruz provinces of Argentina: Diplolaimelloides tehuelchus sp. nov. has four bursal and three postcloacal papillae, short gubernaculum, and spicules longer than 50 μm; it has been found in Patagonian salty lagoons and in high coastal salt marsh areas. Diplolaimelloides warwicki sp. nov. is characterized by the angular shape of the spicules and the long gubernaculum. Diplolaimella ocellata is recorded for the first time in Patagonian salty lagoons and new localities in coastal waters are recorded for Diplolaimella gerlachi Pastor de Ward, 1984.

A COPULA REGRESSION FOR MODELING MULTIVARIATE LOSS TRIANGLES AND QUANTIFYING RESERVING VARIABILITY

2013 ◽  
Vol 44 (1) ◽  
pp. 85-102 ◽  
Author(s):  
Peng Shi
Keyword(s):  
Numerical Analysis ◽  
Estimation Error ◽  
Copula Function ◽  
Predictive Distribution ◽  
Model Error ◽  
Predictive Uncertainty ◽  
Parametric Bootstrapping ◽  
Process Error ◽  
The Family ◽  
The Common

AbstractThis article proposes a claims reserving model for dependent lines of business with the accommodation of association among triangles by a copula function. We show that the family of elliptical copulas is a pretty convenient choice to capture the dependencies introduced by various sources, including the common calendar year effects. To quantify the associated reserving variability, we resort to parametric bootstrapping techniques for simulating the predictive distribution of outstanding liabilities and for calculating the three components of predictive uncertainty: the model error, the process error and the estimation error. Numerical analysis is performed for a portfolio of casualty insurance from a major U.S. insurer.

scholarly journals MEDED - a Novel Interactive Manual to Enhance Psychopharmacologic Care in Children and Adolescents

2009 ◽  
Vol 24 (S1) ◽  
pp. 1-1
Author(s):  
S. Kutcher
Keyword(s):  
Clinical Care ◽  
Opportunity To Learn ◽  
Clinical Settings ◽  
The Novel ◽  
Health Providers ◽  
Adolescent Psychopharmacology ◽  
The Family ◽  
Innovative Tool ◽  
Psychiatric Intervention ◽  
Positive Results

Child and Adolescent psychopharmacology is a complex yet frequently necessary psychiatric intervention that requires the participation of the patient, the family, other caregivers, other health providers as well as physicians. Provision of up to date information about and education in the use of psychotropics is an essential component of good psychopharmacologic treatment. To date there have been no methods of addressing these needs within a collaborative care framework that meets the interests of all parties and optimizes the application of best available knowledge in the clinical care of child and adoelscents with mental disorders. MEDED (C) was developed by a group of pharmacists and child psychiatrists as an innovative tool to meet this need. It has been extensively evaluated in a variety of clinical settings in Canada with substantial positive results. This presentation will review the novel collaborative approach to pharmacotherapy that MEDED supports, the process of MEDED development, the contents of MEDED and research on its use to date. Clinicians and program directors will have the opportunity to learn how to obtain further information abot the use of this tool.

scholarly journals Habitat segregation of Gerres japonicus and G. limbatus in early stages in the Tien Yen estuary, Northern Vietnam

2018 ◽  
Vol 40 (4) ◽  
Author(s):  
Thanh Trung Tran ◽  
Hau Duc Tran ◽  
Nam Hoang Chu ◽  
Thuy Thi Ta
Keyword(s):  
Coastal Waters ◽  
Early Life History ◽  
Nursery Ground ◽  
Middle Reach ◽  
Northern Vietnam ◽  
Early Stages ◽  
The Family ◽  
Salinity Preference ◽  
Commercially Important ◽  
The Difference

Silver-biddy (Gerreidae: Gerres) are commercially important euryhaline fishes in coastal waters; however, information about their distribution during early stages in estuarine environments is poorly known. The present study aims to elucidate the habitat utilization of two species of the family, Gerres japonicus and G. limbatus as larvae and juveniles based on monthly collections from 2013 to 2015 in the Tien Yen estuary, northern Vietnam. They occurred from April to June for G. japonicus and in April and May for G. limbatus with a peak in May for both the species. Both G. japonicus and G. limbatus used the Tien Yen estuary as a nursery ground; however, the former species that colonized the shallows along the banks of the estuary extended to near freshwater habitat, whereas the latter was found at the end of the upper reach and the middle reach. The difference habitat use between the two species in the early life history may be related to their specific salinity preference as G. japonicus prefer lower salinities than G. limbatus.

scholarly journals First record of a reticulated toadfish, Arothron reticularis (Tetraodontiformes: Tetraodontidae), in Korea

2020 ◽  
Vol 23 (1) ◽  
Author(s):  
Chung-Bae Kang ◽  
Sang-Hwa Lee ◽  
Tae-Sik Yu ◽  
Hae-Rim Lee ◽  
Kyeong-Ho Han
Keyword(s):  
Maximum Likelihood ◽  
Coastal Waters ◽  
Morphological Characteristics ◽  
First Record ◽  
The Body ◽  
Single Specimen ◽  
White Line ◽  
Maximum Likelihood Tree ◽  
White Spots ◽  
The Family

AbstractA single specimen of Arothron reticularis (398 mm in total length), belonging to the family Tetraodontidae, was collected in the coastal waters off Hansan island using a set net in May 2019. The morphological characteristics of the specimen are as follows: large eyes and mouth, white spots on the body, and a white line surrounding the eyes in an annular shape. A result of maximum likelihood tree showed that A. reticularis is related to A. hispidus (93.6%). We proposed the Korean name “Geu-murl-mu-nui-kkeo-kkeurl-bok,” in accordance with the characteristics of the specimen.

scholarly journals Taxonomy and morphology of four "ophrys-related" scuticociliates (Protista, Ciliophora, Scuticociliata), with the description of a new genus, Paramesanophrys gen. nov.

2016 ◽  
Author(s):  
Xuming Pan ◽  
Xinpeng Fan ◽  
Saleh A. Al-Farraj ◽  
Shan Gao ◽  
Ying Chen
Keyword(s):  
South China Sea ◽  
South China ◽  
Coastal Waters ◽  
Type Species ◽  
New Genus ◽  
Original Description ◽  
Basal Bodies ◽  
Ciliated Protozoa ◽  
Marine Habitat ◽  
The Family

Generally, “ophrys- related” scuticociliates belong to a specialised group of ciliated protozoa that may act as commensals or pathogens of fishes and crustaceans. In the present study, four “ophrys- taxa” scuticociliates, i.e., Paramesanophrys typica gen. et sp. nov., Mesanophrys carcini (Grolière & Léglise, 1977) Small & Lynn in Aescht, 2001, Metanophrys sinensis Song & Wilbert, 2000, and Metanophrys similis Song et al., 2002, were collected from Chinese coastal waters or mariculture ponds and investigated. Paramesanophrys gen. nov. is assigned to the family Orchitophryidae and differs from its other genera mainly by the position of the paroral membrane relative to membranelle 1–3, i.e., the membrane extends anteriorly to the posterior end of membranelle 3. The type species P. typica gen. et sp. nov., is defined by an elongated body with the posterior end depressed where the caudal cilium is located; 20 or 21 somatic kineties; double-rowed membranelle 1 with eight to ten basal bodies in each kinety; irregularly multi-rowed membranelle 2 and membranelle 3; scutica comprising c. seven or eight kinetosome pairs; a single macronuclear nodule; and marine habitat. The redescription of the three previously known species can be summarized as follows: 1) improved diagnosis is provided for Metanophrys sinensis Song & Wilbert, 2000 based on the original description and the present study; 2) some population-dependent characteristics of our new Mesanophrys carcini isolate are presented; 3) Metanophrys similis, collected from the South China Sea, resembles the original Qingdao population.

scholarly journals A case report of an undiagnosed COVID-19 infection

2020 ◽  
Author(s):  
zhiqian Wu ◽  
haibo Xia ◽  
ronghui zhu ◽  
Jing Cao
Keyword(s):  
Nucleic Acid ◽  
Early Stage ◽  
Diagnostic Process ◽  
Electrolyte Balance ◽  
Disease Treatment ◽  
The Family ◽  
New Type ◽  
Water And Electrolyte Balance ◽  
Coronavirus Infection ◽  
Positive Results

Abstract Background: A very serious new type of coronavirus infection has recently erupted in China. For new viruses, data and information in all aspects need to be improved.Case Presentations: During the early COVID-19 outbreak, a 38-year-old man experienced COVID-19 infection in Shenzhen (nonendemic area-related personnel) and underwent SARS-CoV-2 nucleic acid screening multiple times due to an initial positive COVID-19 screening at the beginning of the disease. The results of these additional tests were negative, and the initial SARS-CoV-2 nucleic acid screening result for this patient was suspected as being a false positive.Diagnosis: After nearly 17 days of hospitalization in two hospitals, a variety of tests were performed to diagnosis this new coronavirus infection (due to the positive results of the initial screening).Intervention: Early in the onset of the disease, treatment was given to maintain water and electrolyte balance, and antibiotics and proprietary Chinese medicines were given as the treatment. The symptoms were controlled at an early stage.Outcomes: The patient developed symptoms on the first day and had pneumonia in the lungs, as shown by a chest CT examination on the 7th day of onset. The subsequent prognosis was good. The family members living with this patient did not show similar symptoms.Conclusion: The diagnostic process of COVID-19 needs to be improved, and the development of SARS-CoV-2 diagnostic reagents needs to be strengthened under certain conditions. Screening should be performed at an early stage when a COVID-19 infection is suspected and should distinguish between patients with a COVID-19 infection and with a non-COVID-19 infection. Patients are more conducive to controlling the COVID-19 epidemic.

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