MiRNA373 induces cervical squamous cell carcinoma SiHa cell apoptosis

Background:Esophageal squamous cell carcinoma (ESCC), the most prevalent histologic subtype of esophageal cancer, is an aggressive malignancy with poor prognosis and a high incidence in the East. Corilagin, an active component present in Phyllanthus niruri L., has been shown to suppress tumor growth in various cancers. However, the effects of corilagin on ESCC and the mechanisms for its tumor suppressive function remain unknown.Methods:Cell proliferation was measured by Cell Counting Kit-8 assay and colony formation assays. Annexin V/PI double-staining was performed to assess cell apoptosis. Immunofluorescence staining and western blotting were used to evaluate the protein expression. A xenograft mice model was used to assess the in vivo antitumor effects of corilagin alone or in combination with cisplatin.Results:We for the first time showed that corilagin was effectively able to inhibit ESCC cell proliferation and induce cell apoptosis. Additionally, our results validated its antitumor effects in vivo using a xenograft mouse model. Mechanistically, we found that corilagin caused significant DNA damage in ESCC cells. We found that corilagin could significantly attenuate the expression of the E3 ubiquitin ligase RING finger protein 8 (RNF8) through ubiquitin-proteasome pathway, leading to the inability of DNA damage repair response and eventually causing cell apoptosis. Furthermore, we also showed that corilagin substantially enhanced the antitumor effects of chemotherapy drug cisplatin both in vitro and in vivo.Conclusion:Our results not only provided novel and previously unrecognized evidences for corilagin-induced tumor suppression through inducing DNA damage and targeting RNF8 in ESCC, but also highlighted that corilagin might serve as an adjunctive treatment to conventional chemotherapeutic drugs in ESCC patients.

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Immunohistochemical staining of podoplanin is helpful in determining the microinvasion of cervical squamous cell carcinoma

Annals of Diagnostic Pathology ◽

10.1016/j.anndiagpath.2020.151493 ◽

2020 ◽

Vol 46 ◽

pp. 151493

Author(s):

Xiaowen Qiao ◽

Yuhong Sun ◽

Ying Zou ◽

Jiaxiang Lin ◽

Wei Xu ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Immunohistochemical Staining ◽

Cervical Squamous Cell Carcinoma

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Screening of genes related to survival prognosis of cervical squamous cell carcinoma and construction of prognosis prediction model

Journal of Obstetrics and Gynaecology Research ◽

10.1111/jog.14827 ◽

2021 ◽

Author(s):

Rui Qin ◽

Lu Cao ◽

Cong Ye ◽

Junrong Wang ◽

Ziqian Sun

Keyword(s):

Squamous Cell Carcinoma ◽

Prediction Model ◽

Cell Carcinoma ◽

Squamous Cell ◽

Cervical Squamous Cell Carcinoma ◽

Survival Prognosis ◽

Prognosis Prediction ◽

Prognosis Prediction Model

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Significance of miR-141 and miR-340 in cervical squamous cell carcinoma

Open Medicine ◽

10.1515/med-2021-0281 ◽

2021 ◽

Vol 16 (1) ◽

pp. 864-872

Author(s):

Wenting Li ◽

Bo Yang ◽

Yiqun Li ◽

Cuicui Wang ◽

Xinzhi Fang

Keyword(s):

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Target Gene ◽

Squamous Epithelium ◽

Figo Stage ◽

Cervical Squamous Cell Carcinoma ◽

Squamous Intraepithelial Lesion ◽

Nerve Invasion ◽

Intraepithelial Lesion

Abstract Background We investigated the expression and clinical significance of miR-141 and miR-340 in cervical squamous cell carcinoma (CSCC). Methods Expression of miR-141 and miR-340 in CSCC, high-grade squamous intraepithelial lesion (HSIL), and normal cervical squamous epithelium were detected by qRT-PCR. PTEN was assessed by immunohistochemistry. Their relationship with clinicopathological features was analyzed. Results The changes of miR-141 and miR-340 were different in CSCC, HSIL, and normal squamous epithelium (P = 0.030). miR-141 expression was statistically significant in gross type, differentiation, uterine corpus invasion, nerve invasion, vagina invasion, and FIGO stage in CSCC (P < 0.05). miR-340 expression was related to tumor size, differentiation, nerve invasion, lymph node metastasis, and FIGO stage in CSCC (P < 0.05). miR-141 and miR-340 expressions were statistically significant in different ages (P < 0.05) in HSIL. The AUC of miR-141 in CSCC diagnosis and that of miR-340 in HSIL diagnosis were 0.893 and 0.764, respectively. The sensitivity and the specificity of miR-141 for diagnosis of CSCC were 95.0% and 60.8%, respectively, while those of miR-340 for diagnosis of HSIL were 90.0 and 48.6%, respectively. miR-141 and miR-340 expressions are associated with PTEN expression (P = 0.002 and P < 0.001). Conclusion miR-141 and miR-340 may be associated with their target gene PTEN and involved in the carcinogenesis of cervical squamous epithelium.

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Pathologic Determination of Multifocality in pT1a1 Squamous Cell Carcinoma of the Cervix: A Retrospective Analysis

International Journal of Surgical Pathology ◽

10.1177/1066896921990726 ◽

2021 ◽

pp. 106689692199072

Author(s):

Jingjing Hu ◽

Kojo R. Rawish ◽

Mariah Leivo ◽

Dennis Adams ◽

Somaye Y. Zare ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Residual Disease ◽

Cervical Squamous Cell Carcinoma ◽

Tnm Staging ◽

Stromal Invasion ◽

Lateral Extent ◽

Clinicopathologic Parameters

When more than one focus of stromal invasion is present in a superficially invasive cervical squamous cell carcinoma (SCC), determination of the tumoral lateral extent/horizontal extension, and hence tumor-nodes-metastases (TNM) staging, can be problematic. In recent years, a diagnostic approach to distinguish multifocal pT1a1 from pT1b cases has gained increased attention. These criteria call for classifying SCC as multifocal when invasive foci are separated by blocks of uninvolved cervical tissues, and/or are located on separated cervical lips in a tumor that is discontinuous, and/or are situated far apart (≥2 mm) from each other. In this study, we assess our experience with multifocal stage pT1a1 cervical SCC that was retrospectively classified as such using these criteria. Slides from the loop electrosurgical excision or conization specimens, comprising 212 pT1a1, 173 pT1a2, and 206 pT1b cases, were reviewed. Twenty-four (11%) of the 212 pT1a1 cases were classified as multifocal after review. The 24 multifocal pT1a1 cases were compared with the 188 unifocal pT1a1 cases regarding a variety of clinicopathologic parameters. Notably, these 2 groups showed no significant differences regarding all parameters that were evaluated, including patient age, recurrence rate, primary tumoral features in the primary excision specimen (rate of positive margins, median depth of stromal invasion, frequency of lymphovascular invasion), and frequency of residual disease in additional excisions. In summary, we demonstrate comparably favorable patient outcomes in both unifocal and multifocal cases of pT1a1 SCC of the cervix, and, accordingly, we conclusively affirm the validity of the aforementioned criteria for establishing multifocality.

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