scholarly journals Intelligent Integrative Platform for Sharing Heterogenuous Stem Cell Research Data

2021 ◽  
Author(s):  
Kirill Borziak ◽  
Irena Parvanova ◽  
Joseph Finkelstein
Keyword(s):  
Stem Cell ◽  
Cancer Stem Cell ◽  
Language Processing ◽  
Stem Cell Research ◽  
Heterogeneous Data ◽  
Research Data ◽  
Data Sets ◽  
Ease Of Access ◽  
Data Elements

Recent studies demonstrated that comparative analysis of stem cell research data sets originating from multiple studies can produce new information and help with hypotheses generation. Effective approaches for incorporating multiple diverse heterogeneous data sets collected from stem cell projects into a harmonized project-based framework have been lacking. Here, we provide an intelligent informatics solution for integrating comprehensive characterizations of stem cells with research subject and project outcome information. Our platform is the first to seamlessly integrate information from iPSCs and cancer stem cell research into a single platform, using a multi-modular common data element framework. Heterogeneous data is validated using predefined ontologies and stored in a relational database, to ensure data quality and ease of access. Testing was performed using 103 published, publicly-available iPSC and cancer stem cell projects conducted in clinical, preclinical and in vitro evaluations. We validated the robustness of the platform, by seamlessly harmonizing diverse data elements, and demonstrated its potential for knowledge generation through the aggregation and harmonization of data. Future aims of this project include increasing the database size using crowdsourcing and natural language processing functionalities. The platform is publicly available at https://remedy.mssm.edu/.

scholarly journals Introducing a Platform for Integrating and Sharing Stem Cell Research Data

2021 ◽  
Author(s):  
Kirill Borziak ◽  
Irena Parvanova ◽  
Joseph Finkelstein
Keyword(s):  
Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Heterogeneous Data ◽  
Knowledge Generation ◽  
Common Data Elements ◽  
Shared Data ◽  
Stem Cell Product ◽  
Induced Pluripotent ◽  
Data Elements

Advancements in regenerative medicine have highlighted the need for increased standardization and sharing of stem cell products to help drive these innovative interventions toward public availability and to increase collaboration in the scientific community. Although numerous attempts and numerous databases have been made to store this data, there is still a lack of a platform that incorporates heterogeneous stem cell information into a harmonized project-based framework. The aim of the platform described in this study, ReMeDy, is to provide an intelligent informatics solution which integrates diverse stem cell product characteristics with study subject and omics information. In the resulting platform, heterogeneous data is validated using predefined ontologies and stored in a relational database. In this initial feasibility study, testing of the ReMeDy functionality was performed using published, publically-available induced pluripotent stem cell projects conducted in in vitro, preclinical and intervention evaluations. It demonstrated the robustness of ReMeDy for storing diverse iPSC data, by seamlessly harmonizing diverse common data elements, and the potential utility of this platform for driving knowledge generation from the aggregation of this shared data. Next steps include increasing the number of curated projects by developing a crowdsourcing framework for data upload and an automated pipeline for metadata abstraction. The database is publically accessible at https://remedy.mssm.edu/.

scholarly journals Isolation and Establishment of a Highly Proliferative, Cancer Stem Cell-Like, and Naturally Immortalized Triple-Negative Breast Cancer Cell Line, KAIMRC2

Cells ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1303
Author(s):  
Rizwan Ali ◽  
Hajar Al Zahrani ◽  
Tlili Barhoumi ◽  
Alshaimaa Alhallaj ◽  
Abdullah Mashhour ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cell ◽  
Cancer Stem Cell ◽  
Cell Line ◽  
Cancer Cell ◽  
Triple Negative Breast Cancer ◽  
Breast Cancer Cell Line ◽  
Triple Negative ◽  
Cancer Cell Line

In vitro studies of a disease are key to any in vivo investigation in understanding the disease and developing new therapy regimens. Immortalized cancer cell lines are the best and easiest model for studying cancer in vitro. Here, we report the establishment of a naturally immortalized highly tumorigenic and triple-negative breast cancer cell line, KAIMRC2. This cell line is derived from a Saudi Arabian female breast cancer patient with invasive ductal carcinoma. Immunocytochemistry showed a significant ratio of the KAIMRC2 cells’ expressing key breast epithelial and cancer stem cells (CSCs) markers, including CD47, CD133, CD49f, CD44, and ALDH-1A1. Gene and protein expression analysis showed overexpression of ABC transporter and AKT-PI3Kinase as well as JAK/STAT signaling pathways. In contrast, the absence of the tumor suppressor genes p53 and p73 may explain their high proliferative index. The mice model also confirmed the tumorigenic potential of the KAIMRC2 cell line, and drug tolerance studies revealed few very potent candidates. Our results confirmed an aggressive phenotype with metastatic potential and cancer stem cell-like characteristics of the KAIMR2 cell line. Furthermore, we have also presented potent small molecule inhibitors, especially Ryuvidine, that can be further developed, alone or in synergy with other potent inhibitors, to target multiple cancer-related pathways.

scholarly journals Forced expression of Nanog with mRNA synthesized in vitro to evaluate the malignancy of HeLa cells through acquiring cancer stem cell phenotypes

2016 ◽  
Vol 35 (5) ◽  
pp. 2643-2650 ◽  
Author(s):  
YAN DING ◽  
AI QING YU ◽  
XIAO LI WANG ◽  
XING RONG GUO ◽  
YA HONG YUAN ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cancer Stem Cell ◽  
Hela Cells ◽  
Cell Phenotypes

658 CD44, Pancreatic Cancer Stem Cell Marker, Positive Cells Are Responsible for the Acquiring Gemcitabine Induced Chemoresistance In Vitro

2008 ◽  
Vol 134 (4) ◽  
pp. A-96
Author(s):  
Sung Pil Hong ◽  
Jeong Youp Park ◽  
Jing Wen ◽  
Jin Wook Yoon ◽  
Kyung Hwa Park ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Stem Cell ◽  
Cancer Stem Cell ◽  
Stem Cell Marker ◽  
Cancer Stem Cell Marker ◽  
Cell Marker

Applications of Advanced Nanotechnology in Stem Cell Research

2021 ◽  
Vol 13 (2) ◽  
pp. 188-198
Author(s):  
Chih-Hui Yang ◽  
Shu-Ling Huang ◽  
Yi-Ting Wang ◽  
Chun-Ho Chang ◽  
Ya-Chi Tsai ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Research ◽  
Biomedical Applications ◽  
Academic Research ◽  
Review Article ◽  
Technical Problems ◽  
Biomedical Industry ◽  
Significant Attention

Nanotechnology gives rise to new breakthroughs and developments in various fields. The applications of advanced nanotechnology may resolve the current technical problems encountered in stem cell research. Nanotechnology has gained significant attention in both academic research and the biomedical industry in recent years. In this mini-review article, the progress of nanotechnology-aided stem cell studies has been surveyed, and the in vitro and in vivo applications of nanotechnology have been introduced. The in vitro studies are divided into three categories: isolation, detection, and regulation. The progress of in vivo studies and trends in biomedical applications have also been addressed.

Creating Life

2021 ◽  
pp. 53-77
Author(s):  
Courtney S. Campbell
Keyword(s):  
Stem Cell ◽  
Stem Cell Research ◽  
Moral Agency ◽  
Human Life ◽  
Genetic Diagnosis ◽  
Sex Selection ◽  
Vitro Fertilization ◽  
Genetic Technologies ◽  
And Gender

This chapter considers ecclesiastical teaching and personal experience regarding new reproductive technologies, such as in vitro fertilization, repro-genetic options, such as preimplantation genetic diagnosis, and stem cell research ethics. The principal argument is that the ethics of medical technologies used to treat and provide remedies for infertility turns primarily on questions of moral agency, familial integrity, and medical professionalism, including safety and efficacy. The Church of Jesus Christ of Latter-day Saints (LDS) procreative imperative can make the experience of infertility a wrenching personal ordeal that necessitates re-storying personal and gender identities relative to spiritual entrustment. Repro-genetic technologies find a moral point through disease prevention but are ethically controversial when their intent is for nonmedical purposes, such as sex selection or enhanced capacities. Despite firm convictions about the sanctity of human life, LDS teaching shows surprising acceptance regarding use of embryos for stem cell research.

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