Tissue quantification of radioiodine thyroid uptake in humans by an isotopic imaging technique on slides

2020 ◽  
pp. 1-13
Author(s):  
Achwak Cherif ◽  
Manel Nouira ◽  
Nicole Barthe ◽  
Radhouane Mani ◽  
Wassim Kermani ◽  
...  
Keyword(s):  
Radioactive Iodine ◽  
Imaging System ◽  
Thyroid Tissue ◽  
Image Resolution ◽  
Good Spatial Resolution ◽  
High Image Resolution ◽  
Thyroid Metabolism ◽  
Iodine 131 ◽  
Volume Unit

BACKGROUND: Thyroid metabolism involves iodine, which allows us to use radioactive iodine for diagnostic and therapy purposes. The efficiency of radioiodine therapy depends on several parameters; the ability of thyroid tissue to uptake radioactive iodine is one of them. OBJECTIVE: The objective of this work is to quantify the radioactive iodine uptake on thyroid tissue. METHODS: In this work, we developed a method to quantify the in vivo uptake of iodine-131 on sections of thyroid glands removed by thyroidectomies. We performed an analysis of histological sections of the thyroid tissue by beta imaging. We had the opportunity to quantify the fixed radioactivity and to analyze its distribution in the thyroid gland, thanks to the good spatial resolution available with the type of detector used. RESULTS: The results gave a high image resolution showing the heterogeneity of iodine-131 fixation by the thyroid tissue. We were able to quantify the tissue radioactivity in mega Becquerel (MBq) per volume unit. CONCLUSION: This work has shown that the direct quantification of the thyroid tissue uptake is possible using the beta imaging system.

scholarly journals In vivo Validation of Reconstruction-Based Resolution Recovery for Human Brain Studies

2009 ◽  
Vol 30 (2) ◽  
pp. 381-389 ◽  
Author(s):  
Jurgen EM Mourik ◽  
Mark Lubberink ◽  
Floris HP van Velden ◽  
Reina W Kloet ◽  
Bart NM van Berckel ◽  
...  
Keyword(s):  
Imaging System ◽  
Volume Of Distribution ◽  
Image Resolution ◽  
Good Recovery ◽  
Good Correspondence ◽  
Resolution Recovery ◽  
Positron Emission ◽  
Quantitative Accuracy ◽  
Spread Function

The aim of this study was to validate in vivo the accuracy of a reconstruction-based partial volume correction (PVC), which takes into account the point spread function of the imaging system. The NEMA NU2 Image Quality phantom and five healthy volunteers (using [11C]flumazenil) were scanned on both HR+ and high-resolution research tomograph (HRRT) scanners. HR+ data were reconstructed using normalization and attenuation-weighted ordered subsets expectation maximization (NAW-OSEM) and a PVC algorithm (PVC-NAW-OSEM). HRRT data were reconstructed using 3D ordinary Poisson OSEM (OP-OSEM) and a PVC algorithm (PVC-OP-OSEM). For clinical studies, parametric volume of distribution ( VT) images were generated. For phantom data, good recovery was found for both OP-OSEM (0.84 to 0.97) and PVC-OP-OSEM (0.91 to 0.98) HRRT reconstructions. In addition, for the HR+, good recovery was found for PVC-NAW-OSEM (0.84 to 0.94), corresponding well with OP-OSEM. Finally, for clinical data, good correspondence was found between PVC-NAW-OSEM and OP-OSEM-derived VT values (slope: 1.02±0.08). This study showed that HR+ image resolution using PVC-NAW-OSEM was comparable to that of the HRRT scanner. As the HRRT has a higher intrinsic resolution, this agreement validates reconstruction-based PVC as a means of improving the spatial resolution of the HR+ scanner and thereby improving the quantitative accuracy of positron emission tomography.

Effect of acetazoleamide on thyroid metabolism

1959 ◽  
Vol 196 (2) ◽  
pp. 291-294 ◽  
Author(s):  
D. T. Krieger ◽  
A. Moses ◽  
H. Ziffer ◽  
J. L. Gabrilove ◽  
L. J. Soffer
Keyword(s):  
Carbonic Anhydrase ◽  
Radioactive Iodine ◽  
Thyroid Tissue ◽  
Inhibitory Effect ◽  
Iodine Uptake ◽  
Human Thyroid ◽  
Inhibitory Potency ◽  
Thyroid Metabolism ◽  
Radioactive Iodine Uptake

Previous studies showed Diamox (acetazoleamide) to be an effective depressant of thyroidal radioactive iodine uptake. It was hypothesized that this effect was mediated via carbonic anhydrase inhibition. The presence of carbonic anhydrase was demonstrated in rat and human thyroid tissue. Diamox and other sulfonamide like drugs were tested for their relative carbonic anhydrase inhibitory potency and depressant effect on in vitro radioactive iodine uptake. No correlation between these two effects could be observed, and carbonic anhydrase addition did not reverse the inhibitory effect of Diamox. These experiments therefore fail to provide an explanation of the mechanism of depression of thyroidal radioactive iodine uptake by Diamox.

scholarly journals Misdiagnosis of Paraganglioma by 123I-mIBG Without Stable Iodine Blockade of Thyroidal Radioiodine Uptake

2020 ◽  
Vol 4 (9) ◽  
Author(s):  
Stanley M Chen Cardenas ◽  
Daisy Duan ◽  
Lisa M Rooper ◽  
Prasanna Santhanam ◽  
David S Cooper ◽  
...  
Keyword(s):  
Radioactive Iodine ◽  
Thyroid Tissue ◽  
Elevated Plasma ◽  
Radioiodine Uptake ◽  
Catecholamine Excess ◽  
Iodine 131 ◽  
Iodine 123 ◽  
High Doses ◽  
The Masses ◽  
Stable Iodine

Abstract Iodine-123/iodine-131 (123I/131I)-metaiodobenzylguanidine (mIBG) scan is an established tool for the localization and treatment of neuroendocrine tumors such as paragangliomas (PGL). To minimize thyroid irradiation by the radioactive iodine in the mIBG preparation, blockade of thyroidal iodine uptake with high doses of stable iodine used to be given routinely as part of all mIBG protocols. As 123I is now more frequently utilized than 131I, concern about thyroid radiation has lessened and thyroid blockade is often considered unnecessary. However, in certain situations, the lack of thyroid blockade can significantly impact treatment decisions. This report describes 2 patients who had mediastinal masses incidentally discovered on CT scans, and on further evaluation were found to have symptoms suggesting catecholamine excess with mildly elevated plasma normetanephrine levels. 123I-mIBG scans were performed without thyroid blockade, which demonstrated accumulation of tracer in the masses that were therefore deemed positive for PGL. Both patients underwent surgical resection of the masses with their surgical pathology revealing ectopic thyroid tissue (ETT). These cases illustrate that if appropriate thyroid blockade is not performed, ETT concentrating radioiodine from mIBG can lead to falsely positive mIBG scans and unnecessary surgical procedures. We conclude that in the setting of a mass suspicious for PGL in a location potentially representing ETT, such as the mediastinum, thyroid blockade should be employed for mIBG protocols to avoid false positive scans caused by ETT.

An angiographic method for in vivo study of arteries of the circle of Willis in small animals

1992 ◽  
Vol 263 (5) ◽  
pp. H1616-H1622 ◽  
Author(s):  
D. R. Harder ◽  
M. L. Schulte ◽  
A. V. Clough ◽  
C. A. Dawson
Keyword(s):  
Imaging System ◽  
Circle Of Willis ◽  
External Carotid ◽  
Small Animals ◽  
X Ray ◽  
Small Vessels ◽  
Good Spatial Resolution ◽  
X Ray Imaging ◽  
X Ray Absorption

An X-ray imaging technique designed to allow sequential diameter measurements of the cerebral vessels in intact, anesthetized small animals under relatively physiological conditions is described. The ferret and the rabbit were chosen as potentially useful animal models for studying the cerebrovascular system because of the advantageous anatomic characteristics of these relatively small species. A commercially available and relatively inexpensive X-ray imaging system with a small focal spot provides good spatial resolution. An external carotid perfusion loop allows for 1) the introduction of low-osmolality contrast medium without changing perfusion pressure or flow and 2) measurement of internal carotid and circle of Willis pressures at the same time that the vessel images are obtained. In the present study, detection of small changes in the diameters of the small vessels is facilitated by an algorithm utilizing the X-ray absorption by the entire vessel cross section. This avoids some of the problems of edge detection for small cylindrical vessels wherein the contrast is less than optimal and diminishes as the vessel perimeter is approached.

Multi-mode light microscopy of microtubule assembly dynamics and chromosome movement in vivo and In vitro

1996 ◽  
Vol 54 ◽  
pp. 730-731
Author(s):  
E. D. Salmon ◽  
J. C. Waters ◽  
C. Waterman-Storer
Keyword(s):  
Imaging System ◽  
Ccd Camera ◽  
Transmitted Light ◽  
Microtubule Assembly ◽  
Live Cells ◽  
Optical Efficiency ◽  
Multiple Band ◽  
Multi Mode

We have developed a multi-mode digital imaging system which acquires images with a cooled CCD camera (Figure 1). A multiple band pass dichromatic mirror and robotically controlled filter wheels provide wavelength selection for epi-fluorescence. Shutters select illumination either by epi-fluorescence or by transmitted light for phase contrast or DIC. Many of our experiments involve investigations of spindle assembly dynamics and chromosome movements in live cells or unfixed reconstituted preparations in vitro in which photodamage and phototoxicity are major concerns. As a consequence, a major factor in the design was optical efficiency: achieving the highest image quality with the least number of illumination photons. This principle applies to both epi-fluorescence and transmitted light imaging modes. In living cells and extracts, microtubules are visualized using X-rhodamine labeled tubulin. Photoactivation of C2CF-fluorescein labeled tubulin is used to locally mark microtubules in studies of microtubule dynamics and translocation. Chromosomes are labeled with DAPI or Hoechst DNA intercalating dyes.

TUMOURS OF THE HYPOPHYSIS AND THE THYROID IN MICE

1955 ◽  
Vol 18 (4) ◽  
pp. 445 ◽  
Author(s):  
O. Mühlbock
Keyword(s):  
Thyroid Gland ◽  
Radioactive Iodine ◽  
Thyroid Tissue ◽  
Prolonged Treatment ◽  
Genetic Constitution ◽  
Thiouracil Derivatives

SUMMARY Hypophyseal tumours in mice develop after prolonged treatment with oestrogens, after injection of radioactive iodine in doses which destroy the thyroid tissue and after prolonged treatment with thiouracil-derivatives. The tumours of the hypophysis only occur in certain strains of mice and hence the genetic constitution is of importance. The origin of this difference between strains has hitherto remained completely obscure. The hypophyseal tumours in mice seen following administration of oestrogens and those observed after destruction of the thyroid gland are morphologically similar. They consist of cells staining blue to varying degrees, and they may thus be termed amphophile tumours. The possible mechanism of development of the hypophyseal tumours has been discussed.

Methodology for TEM Analysis of Barrier Profiles

2002 ◽  
Author(s):  
Tan-Chen Lee ◽  
Jui-Yen Huang ◽  
Li-Chien Chen ◽  
Ruey-Lian Hwang ◽  
David Su
Keyword(s):  
Sample Preparation ◽  
Profile Analysis ◽  
Image Interpretation ◽  
Sample Thickness ◽  
Image Resolution ◽  
Specimen Preparation ◽  
Tem Analysis ◽  
Preparation Methods ◽  
High Image Resolution ◽  
Common Technique

Abstract Device shrinkage has resulted in thinner barriers and smaller vias. Transmission Electron Microscopy (TEM) has become a common technique for barrier profile analysis because of its high image resolution. TEM sample preparation and image interpretation becomes difficult when the size of the small cylindrical via is close to the TEM sample thickness. Effects of different sample thickness and specimen preparation methods, therefore, have been investigated. An automatic FIB program has been shown to be useful in via sample preparation. Techniques for imaging a TEM specimen will be discussed in the paper. Conventional TEM bright field (BF) image is adequate to examine the barrieronly via; however, other techniques are more suitable for a Cu filled via.

scholarly journals Nanotechnology: from In Vivo Imaging System to Controlled Drug Delivery

2017 ◽  
Vol 12 (1) ◽  
Author(s):  
Maria Mir ◽  
Saba Ishtiaq ◽  
Samreen Rabia ◽  
Maryam Khatoon ◽  
Ahmad Zeb ◽  
...  
Keyword(s):  
Drug Delivery ◽  
In Vivo Imaging ◽  
Imaging System ◽  
Controlled Drug Delivery ◽  
In Vivo Imaging System

scholarly journals MSCs-engineered biomimetic PMAA nanomedicines for multiple bioimaging-guided and photothermal-enhanced radiotherapy of NSCLC

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Yipengchen Yin ◽  
Yongjing Li ◽  
Sheng Wang ◽  
Ziliang Dong ◽  
Chao Liang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Membranes ◽  
Imaging System ◽  
Fluorescence Signal ◽  
Bimodal Imaging ◽  
Red Blood Cell Membranes ◽  
Magnetic Resonance Imaging Mri ◽  
Tumor Accumulation

Abstract Background The recently developed biomimetic strategy is one of the mostly effective strategies for improving the theranostic efficacy of diverse nanomedicines, because nanoparticles coated with cell membranes can disguise as “self”, evade the surveillance of the immune system, and accumulate to the tumor sites actively. Results Herein, we utilized mesenchymal stem cell memabranes (MSCs) to coat polymethacrylic acid (PMAA) nanoparticles loaded with Fe(III) and cypate—an derivative of indocyanine green to fabricate Cyp-PMAA-Fe@MSCs, which featured high stability, desirable tumor-accumulation and intriguing photothermal conversion efficiency both in vitro and in vivo for the treatment of lung cancer. After intravenous administration of Cyp-PMAA-Fe@MSCs and Cyp-PMAA-Fe@RBCs (RBCs, red blood cell membranes) separately into tumor-bearing mice, the fluorescence signal in the MSCs group was 21% stronger than that in the RBCs group at the tumor sites in an in vivo fluorescence imaging system. Correspondingly, the T1-weighted magnetic resonance imaging (MRI) signal at the tumor site decreased 30% after intravenous injection of Cyp-PMAA-Fe@MSCs. Importantly, the constructed Cyp-PMAA-Fe@MSCs exhibited strong photothermal hyperthermia effect both in vitro and in vivo when exposed to 808 nm laser irradiation, thus it could be used for photothermal therapy. Furthermore, tumors on mice treated with phototermal therapy and radiotherapy shrank 32% more than those treated with only radiotherapy. Conclusions These results proved that Cyp-PMAA-Fe@MSCs could realize fluorescence/MRI bimodal imaging, while be used in phototermal-therapy-enhanced radiotherapy, providing desirable nanoplatforms for tumor diagnosis and precise treatment of non-small cell lung cancer.

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