scholarly journals COMPARATIVE CHARACTERISTICS OF CHANGES IN HEMO - AND LYMPHOMICROCIRCULATORY VESSELS OF ILEUM ACCUMULATED LYMPHOID NODES OF RATS IN THE CASE OF APPLICATION OF INFUSION SOLUTIONS IN EXPERIMENTAL BURN DISEASE

2021 ◽  
Vol 17 (2) ◽  
pp. 3-11
Author(s):  
I.V. Dzevulska ◽  
R.M. Matkivska ◽  
A.M. Sinitska ◽  
L.V. Prysiazhnjuk ◽  
A.Y. Yanchyshyn
Keyword(s):  
Sodium Chloride ◽  
Chloride Solution ◽  
Sodium Chloride Solution ◽  
Vascular Wall ◽  
The Body ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Isotonic Sodium Chloride ◽  
Hyperosmolar Solutions ◽  
Burn Disease

Relevance. Thermal burns of the skin cause the development of burns, the main factor of which is endogenous intoxication. The infusion of detoxification solutions is a mandatory component of the treatment of burns, as it corrects its course and prevents the development of certain stages and complications. According to the stage of the burn disease and the different direction of its links in its pathogenesis, infusion therapy should not only restore water-electrolyte balance and detoxify the body, but also contribute to the normalization and stabilization of vital (including immune) body functions. Objective: study of changes in the vessels of the hemo- and lymphomicrocirculatory system of Peyer's patches in burnt rats at the stages of burn disease, under conditions of intravenous infusion of isotonic sodium chloride solution and combined colloidal-hyperosmolar solutions (lactoprotein and sorbitolum). Materials and methods. The experiment was performed on 72 white rats. Experimental animals were divided into 4 groups (18 animals in each group). I, II, III - rats with skin burn trauma, which received a separate infusion of isotonic sodium chloride (I), lactoprotein with sorbitol (II) and hecoton (III), at a dose of 10 ml / kg. IV - intact animals (control group). Simulation of burn disease was performed by inflicting burn injury by applying to the lateral surfaces of the body of animals four copper plates, which were previously kept for 6 minutes in water with a constant temperature of 100 ° C. Histological and electron microscopic examination were performed. Light microscopy was used. Results. Intravenous administration of the applied infusion solutions caused various phase changes of the hemo- and lymphomicrocirculatory tract of Peyer's patches of the ileum of burnt rats, depending on the applied solution. In the case of infusion of burnt rats with 0.9% sodium chloride solution, the negative effects of burn disease were exacerbated, which were manifested by damage to the vascular wall of the hemo- and lymphocapillary channels, resulting in plasma and lymph seepage through the affected areas into the perivascular space. This process was accompanied by a violation of the rheological properties of blood, the formation of edema. The established "membrane-plastic effect" of lactoprotein with sorbitol revealed clear organ-specific features, which had manifestations of penetration through the damaged wall of microvascular electron-dense impregnations, which contributed to a significant thickening of the basement membrane in the wall of the blood capillary formation and its gradual formation of rounded membranous structure. This structure of variable electron density not only strengthened the vascular wall, but also served as a closure, helping to close the vascular lumen at the site of largest damage. The difference between the perinodal lymphatic capillaries in burnt rats infused with lactoprotein and sorbitol is characterized by the phenomenon of "overflow" of the lymphocapillary channel with cells in different functional states. In the case of infusion of hecoton solution, the effect of "overflow" of the lymphocapillary channel was not observed, which indicates adequate implementation of the immune function of immunocompetent cells. Conclusions. Intravenous infusion of colloid-hyperosmolar solutions causes various changes in the hemo- and lymphomicrocirculatory tract of Peyer's patches. Infusion of a  0.9% sodium chloride solution leads to a deepening of the negative consequences of burn disease, which manifests itself in the form of damage to the vascular wall of the hemo- and lymphocapillary channels. Infusion of solutions of lactoprotein with sorbitol and hecoton helps to preserve the vascular wall of the nodular lymphatic capillaries. When a solution of lactoprotein with sorbitol is applied around the damaged vessels of blood capillaries and venules, annular membrane formations are formed, which promote selective recirculation of structurally intact lymphocytes.

scholarly journals Reactive and destructive changes of Peyer’s patches in rats with experimental burn disease under infusion of detoxification solutions

2019 ◽  
Vol 25 (2) ◽  
pp. 56-63
Author(s):  
V.G. Cherkasov ◽  
R.M. Matkivska ◽  
E.V. Cherkasov ◽  
R.F. Kaminskyi ◽  
L.M. Yaremenko
Keyword(s):  
Endoplasmic Reticulum ◽  
Dendritic Cells ◽  
Intravenous Infusion ◽  
Burn Injury ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Experimental Burn ◽  
Isotonic Sodium Chloride ◽  
Hyperosmolar Solutions ◽  
Burn Disease

The pathogenesis of burn immune dysfunction and burn enteropathy needs further clarification given that the cellular lesions of lymphoid tissue associated with mucous membranes are the least studied. The purpose of the study was to establish reactive and destructive changes in Peyer’s patches of rats after burn injury of the skin with the use of intravenous infusion of isotonic sodium chloride solution and combined colloid-hyperosmolar solutions. White male rats weighing 160-200 g at 6 months of age were divided into 4 groups (18 animals in each group): I, II, III – rats with burn skin injury (grade II-III burn with an area of 23% of body surface area and the development of moderate-severity shock state) which was administered a separate intravenous infusion once a day for the first 7 days of the experiment with isotonic sodium chloride, lactoprotein with sorbitol and HAES-LX-5%, in each case at a dose of 10 ml/kg; IV – intact animals. The material was collected from rats under deep thiopental intraperitoneal anesthesia after 1, 3, 7, 14, 21 and 30 days after burn injury. Biopsies from Peyer’s patches for histological and electron microscopic examination were processed using conventional methods. Investigation of histological preparations stained with hematoxylin-eosin was performed on an Olympus BX51 microscope. Ultrathin sections were contrasted on copper support meshes with uranyl acetate and lead citrate according to Reynolds and studied using a PEM-125K electron microscope. Electron and light microscopy data indicate that intravenous infusion of colloidal-hyperosmolar solutions (lactoprotein with sorbitol and HAES-LX-5%) promotes suppression of inflammatory response, inhibits necrosis, and optimizes lymphoid apoptosis at Peyer’s patches of rats with experimental burn disease caused by burn injury to the skin of 21-23% of the body surface. Apoptotic lymphocytes and their apoptotic bodies are effectively phagocytosed by macrophages and are digestible in heterophagolysosomes. The apoptotic altered dendritic cells in Peyer’s patches are characterized by osmiophilic cytoplasm and a nucleus with high electron density amorphous nucleoplasm. In the cytoplasm are located mitochondria with enlightened matrix and destroyed cristae, irregularly expanded tubules of variable configuration of a granular endoplasmic reticulum with electronically transparent content and numerous derivatives of their vacuole transformation, which are sharply darkened. The fusion of vacuoles leads to the formation of large electron-luminous cavities filled with various residues of compacted degraded cellular structures. Vacualization promotes site segmentation of condensed cytoplasm of apoptotic dendritic cells and formation of apoptotic blebs, which are subject to entrapment and subsequent sequential degradation with the participation of neighboring macrophages. The structural changes of the organelles of the protein-synthesizing apparatus found in Peyer’s plaque cells in rats with experimental burn disease can be regarded as a manifestation caused by functional overload of the granular endoplasmic reticulum (ER-stress). The consequence of optimal development of ER-stress and subsequent unfolded protein response is the apoptotic degradation of the corresponding cell, the course of which is modified by the use of colloid-hyperosmolar solutions.

scholarly journals Effects of Granisetron on Spinal Anesthesia-induced Hypotension

2021 ◽  
Author(s):  
Cem Koray Çataroğlu ◽  
Alp Alptekin ◽  
Aysel Gezer ◽  
Murat Sayın ◽  
Aslı Dönmez
Keyword(s):  
Heart Rate ◽  
Sodium Chloride ◽  
Spinal Anesthesia ◽  
Chloride Solution ◽  
Sodium Chloride Solution ◽  
Elective Surgery ◽  
Demographic Data ◽  
Hyperbaric Bupivacaine ◽  
Significant Difference ◽  
Isotonic Sodium Chloride

Objective: It was aimed to evaluate the effect of intravenous (IV) granisetron used for nausea and vomiting prophylaxis on hypotension and bradycardia caused by spinal anesthesia. Methods: 120 ASA 1-2 patients undergoing elective surgery under spinal anesthesia were randomly divided into Group G (Ganisetron; n=60) and Group P (Placebo; n=60) groups. Five minutes before spinal anesthesia, Group G received 1 mg intravenous granisetron diluted in 10 mL of isotonic sodium chloride solution and Group P received 10 mL of isotonic sodium chloride solution. Spinal anesthesia with hyperbaric bupivacaine 0.5%, 15 mg at the level of L4-5 was applied for both groups. Hemodynamic data, sensory and motor block parameters were recorded before and after spinal anaesthesia every 5 minutes during 20 minutes of surgery. Results: There was no difference in the demographic data of both groups. Although hemodynamic data showed a decrease in both groups according to initial values, blood pressure measurements in group G were significantly higher than the first measure values. There was no significant difference in heart rate values between the groups. Conclusion: Intravenous granisetron reduces hypotension after spinal anesthesia, but it has no significant effect on heart rate.

Endotracheal Suctioning With or Without Instillation of Isotonic Sodium Chloride Solution in Critically Ill Children

2003 ◽  
Vol 12 (3) ◽  
pp. 212-219 ◽  
Author(s):  
Debra A. Ridling ◽  
Lynn D. Martin ◽  
Susan L. Bratton
Keyword(s):  
Sodium Chloride ◽  
Oxygen Saturation ◽  
Endotracheal Tube ◽  
Critically Ill ◽  
Nosocomial Pneumonia ◽  
Chloride Solution ◽  
Sodium Chloride Solution ◽  
Critically Ill Children ◽  
Endotracheal Tubes ◽  
Isotonic Sodium Chloride

• Background Instillation of isotonic sodium chloride solution for endotracheal tube suctioning is controversial. Research has focused on the effect of such instillation in adults; no studies in children have been published.• Objectives (1) To describe differences in oxygen saturation depending on whether or not isotonic sodium chloride solution is instilled during suctioning and (2) to describe the rates of occlusion of endotracheal tubes and nosocomial pneumonia.• Methods A convenience sample of 24 critically ill patients were enrolled before having suctioning and after informed consent had been given. Ages ranged from 10 weeks to 14 years. Patients were randomized to 1 of 2 groups. In group 1, subjects received between 0.5 and 2.0 mL of isotonic sodium chloride solution, depending on their age, once per suctioning episode. In group 2, subjects received no such solution. A total of 104 suctioning episodes were analyzed. Oxygen saturation was recorded at predetermined intervals before and for 10 minutes after suctioning. Occlusion of endotracheal tubes and rates of nosocomial pneumonia also were compared.• Results Patients who had isotonic sodium chloride solution instilled experienced significantly greater oxygen desaturation 1 and 2 minutes after suctioning than did patients who did not. No occlusions of endotracheal tubes and no cases of nosocomial pneumonia occurred in either group.• Conclusions Results of this study support a growing body of evidence that instillation of isotonic sodium chloride solution during endotracheal tube suctioning may not be beneficial and actually may be harmful.

scholarly journals QUANTITATIVE DISTRIBUTION OF PARTICULATE MATERIAL (MANGANESE DIOXIDE) ADMINISTERED INTRAVENOUSLY TO THE CAT

1921 ◽  
Vol 33 (1) ◽  
pp. 77-98 ◽  
Author(s):  
Cecil K. Drinker ◽  
Louis A. Shaw
Keyword(s):  
Blood Pressure ◽  
Sodium Chloride ◽  
Manganese Dioxide ◽  
Vascular Endothelium ◽  
Chloride Solution ◽  
Sodium Chloride Solution ◽  
Particulate Material ◽  
The Body ◽  
Quantitative Distribution ◽  
Intravenous Injections

1. Manganese dioxide suspended in an acacia-sodium chloride solution provides a non-toxic injection which in the present experiments has contained no particles larger than 1µ and which, when deposited in the body, can be determined quantitatively and seen microscopically. 2. Intravenous injections have been made under precautions which preclude removal from the blood or deposition in organs through simple capillary blockage. 3. In nine experiments out of thirteen the circulating blood contained no manganese after 18 minutes. In the four remaining instances there was a steady slight elimination which was incomplete at the end of 1 hour. Within certain limits the rate of removal from the circulating blood and the sites of deposition in the animal are not influenced by the concentration of the suspension, the blood pressure, or antecedent introduction of acacia or histamine. 4. In the cat amounts of manganese dioxide varying between 9.8 and 3.9 mg. of manganese and containing from 50,000,000,000 to 10,000,000,000 particles, if injected intravenously, permit recovery at the end of 1 hour of 90 per cent of the material in the lungs, liver, and spleen in the following proportions: lungs 47 per cent; liver 38.3 per cent; spleen 4.3 per cent. 5. These experiments, coupled with correlative results by other investigators, make it clear that in certain organs—the lungs, liver, and spleen of the cat—the vascular endothelium possesses phagocytic power rendering the capillaries permeable to particulate material as well as to gases, liquids, and dissolved substances.

scholarly journals Peculiarities of the wound healing process in the acute period of burn disease depending on the antiseptic used

2019 ◽  
Vol 2 (2) ◽  
pp. 14-22
Author(s):  
Vasyl Nahaichuk ◽  
Oleksandr Nazarchuk ◽  
Roman Chornopyshchuk ◽  
Pavlo Hormash ◽  
Yuliana Babina
Keyword(s):  
Wound Healing ◽  
Sodium Chloride ◽  
Granulation Tissue ◽  
Chloride Solution ◽  
Sodium Chloride Solution ◽  
Povidone Iodine ◽  
Pathogenic Microorganisms ◽  
Wound Surface ◽  
Acute Period ◽  
Burn Disease

Background. Burn injury, as one of the most common types of trauma, requires a comprehensive approach to treatment, including surgical and conservative treatment with effective post-burn wound protection. One of the main obstacles to wound healing is purulent-infectious complications, for prevention of which antiseptics are widely used. The aim is to study the microbiological, histological features of the wound healing course in the acute period of burn disease with the use of various antiseptics. Materials and methods. In the research there were involved 44 patients with burns (burn area 10–20 % of the body surface), treated in the Clinical Center for Thermal Injury and Plastic Surgery of Communal Non-Commercial Enterprise “Vinnytsia Regional Clinical Hospital named after M. I. Pirogov” (2018–2019). Depending on the antiseptic therapy, these patients were divided into three observation groups. Patients in the 1st study group (n = 15; mean age 48.07 ± 12.05 years) were treated with 2.0 % povidone-iodine during dressing. In the 2nd group (n = 15; mean age – 48.53 ± 14.76 years) antiseptic based on 0.02 % decamethoxin was used. In patients of the comparison group (n = 14; mean age – 47.71 ± 12.39 years) a solution of 10.0 % NaCl was administrated for topical wound management. Microbiological assessment of the condition of the wound surface (on 3, 7, 14, 21 days) and histological examination of tissue samples from the bottom of the wound (on 3, 7, 14 days) were carried out by means of standard methods. Results. Under the use of antiseptic agents based on 2.0 % povidone-iodine and 0.02 % decamethoxin during the treatment period in patients with burns up to 14 days, an effective reduction in the number of conditionally pathogenic microorganisms on the wound surface to lg (4.8 ± 0.4) and lg (3.06 ± 0.5) CFU/ml, respectively, was registered and it was significantly 1.5 times lower than that based on 10 % sodium chloride solution (p < 0.05). When applying decamethoxin for 14 days at the same time as the eradication of conditionally pathogenic microorganisms, moderate colonization of wounds was registered by representatives of normal skin microbiota (Corynebacterium spp., S. epidermidis). Histologically, there was found that with the use of 2.0 % povidone-iodine, the wound healing course was accompanied by a marked decrease in inflammation signs, as well as a pronounced inhibition of the formation of granulation tissue (10–14 days). The use of 0.02 % decamethoxin was accompanied by the formation of granulation tissue of sufficient maturity for atodermoplasty, in the presence of signs of inflammatory cellular reaction with the presence in the superficial parts of the bottom of the post-burn wounds of the fibrinoid layer, with signs of permanent formation of new hemocapillaries in it, surrounded by a thin argyrophilic mesh. When using a hypertonic sodium chloride solution before autodermoplasty, the granulation tissue had signs of uneven inflammatory cell infiltration. Signs of leukocyte-necrotic tissue changes, fibrinoid-leukocyte layers with fragments of tissue detritus, signs of resuscularization of granulation tissue were recorded. Conclusion. Microbiological data strongly suggest the efficacy of 0.02 % decamethoxin and 2.0 % povidone-iodine in reducing microbial wound colonization infection compared to 10.0 % NaCl. The use of antiseptics and hypertonic sodium chloride solution in the acute period of burn disease provides a marked reduction in histological signs of inflammation in the wound with a noticeable positive effect of decamethoxin and 10.0 % NaCl on the formation of granulations and their revascularization compared with povidone-iodine (p < 0.001).

scholarly journals Combination of Zerumbone and Cisplatin to Treat Cervical Intraepithelial Neoplasia in Female BALB/c Mice

2009 ◽  
Vol 19 (6) ◽  
pp. 1004-1010 ◽  
Author(s):  
Ahmad Bustamam Abdul ◽  
Siddig Ibrahim Abdelwahab ◽  
Johari Bin Jalinas ◽  
Adel Sharaf Al-Zubairi ◽  
Manal Mohamed Elhassan Taha
Keyword(s):  
Sodium Chloride ◽  
Cervical Intraepithelial Neoplasia ◽  
Interleukin 6 ◽  
Chloride Solution ◽  
Sodium Chloride Solution ◽  
Antitumor Effect ◽  
Intraepithelial Neoplasia ◽  
Nuclear Antigen ◽  
Isotonic Sodium Chloride

Recent in vitro and in vivo studies have demonstrated that zerumbone (ZER) possesses anticancer properties. The main objective of this study was to examine the effectiveness of the combination of ZER and cisplatin (CIS) to treat cervical intraepithelial neoplasia (CIN) in vivo. Microculture tetrazolium assay and immunohistochemistry of proliferating cellular nuclear antigen were used to study the antitumor effect of ZER. Prenatally exposed female BALB/c mice were used as a model. The progenies with CIN were injected peritoneally with isotonic sodium chloride solution (positive control), CIS, ZER, and a combination of both compounds. All treated and untreated mice were humanely killed, and serum and cervix were obtained for interleukin 6 analysis and histopathologic studies using hematoxylin and eosin staining, respectively. Zerumbone has revealed an antitumor effect on human cervical cancer cells and downregulates immunoexpression of proliferating cellular nuclear antigen (P < 0.05). In vivo study indicates that ZER at 16 mg/kg and CIS at 10 mg/kg have a regressing effect on CIN. The combination of ZER and CIS also showed similar effectiveness in regressing CIN. Our results indicate that the combination of ZER and CIS has modulated the serum level of interleukin 6 when compared with that in mice treated with isotonic sodium chloride solution (P < 0.05). The effectiveness of combining ZER and CIS could be further explored as a new therapeutic intervention of early precancerous stages of carcinogenesis before the invasive stage begins.

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