Comparative efficacy of antioxidant drugs in an in vitro experiment

The aim of this work was to study the antioxidant activity of 3-hydroxypyridine derivatives: Mexidol-Vet® and Emycidin in a model oxidation system. Using the chemiluminescence method, it was found that the introduction of Mexidol-Vet® into the test system at a dose of 1 mg reliably suppresses the amplitude of a fast flash and decreases the formation of photons (light sum value), compared with the test system / placebo and the test system / Emicidin. The antioxidant activity of Mexidol-Vet® is significantly higher than Emicidin by 28.1%.

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“EFFECT OF HOMOEOPATHIC MEDICINE TRIBULUS TERRESTRIS Q, 6C, 12C, 30C, 200C, 1M AS AN INHIBITOR OF CALCIUM OXALATE AND CALCIUM PHOSPHATE CRYSTALLIZATION IN VITRO STUDY”

10.36106/ijsr/3404116 ◽

2021 ◽

pp. 45-48

Author(s):

Sharada Shankar Gowda ◽

Tanveer A. Khan ◽

Ajay Namdeo ◽

Chetan H. Shinde

Keyword(s):

Antioxidant Activity ◽

Calcium Phosphate ◽

Calcium Oxalate ◽

Radical Scavenging ◽

Medical Attention ◽

Tribulus Terrestris ◽

Vitro Experiment ◽

In Vitro Experiment ◽

Maximum Inhibition

Background: Urolithiasis is one of the common conditions in the society and it needs medical attention due to its increase in prevalence. The use of the homeopathic medicines has found to be,of great importance in the treatment of urolithiasis and certainly homoeopathy is a promising eld in this condition. Objectives: The aim of this study was to evaluate the mechanism of urolithiasis and the inhibitory action of homeopathic drug Tribulus terrestris by in vitro experiment. Materials and Method: Homoeopathic preparation of Tribulus terrestris Q, 6C, 12C, 30C, 200C, 1M was planned to evaluate in vitro calcium oxalate and calcium phosphate crystallization using spectro-photometric and colorimetric assay respectively. Considering the role of reactive oxygen species as one of the etiological factors in stone formation, effective antioxidant activity of Tribulus terrestris was also performed by 2,2- diphenyl -1-picrylhydrazyl (DPPH) free radical scavenging assay. Result: Tribulus terrestris Q, 200C and 1M exerted maximum inhibition as 33.62%, 23.89% and 23.00% respectively to calcium oxalate nucleation assay whereas, Tribulus terrestris Q, 6C, 12C, 30C, 200C, 1M exerted maximum inhibition to calcium oxalate aggregation assay up to 76.19%. Tribulus terrestris 12C and 30C showed maximum inhibition as 82.28% and 16. 21% to calcium and phosphate ions respectively. Presence of antioxidant activity by DPPH radical assay for Tribulus terrestris Q and 12C which showed percentage inhibition as 33.11% and 0.95% respectively. Conclusions:Homoeopathic preparation Tribulus terrestris has potential effect on inhibition of calcium oxalate and calcium phosphate crystallization and also homoeopathic preparation of Tribulus terrestris is capable of showing presence of phytochemicals; anti-oxidant activity when performed by in vitro experiment.

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In Silico and in Vitro Evaluation of Deamidation Effects on the Stability of the Fusion Toxin DAB389IL-2

Current Proteomics ◽

10.2174/1570164616666190131150033 ◽

2019 ◽

Vol 16 (4) ◽

pp. 307-313 ◽

Cited By ~ 2

Author(s):

Nasrin Zarkar ◽

Mohammad Ali Nasiri Khalili ◽

Fathollah Ahmadpour ◽

Sirus Khodadadi ◽

Mehdi Zeinoddini

Keyword(s):

In Silico ◽

Catalytic Domain ◽

Md Simulations ◽

Special Importance ◽

Native Form ◽

Vitro Experiment ◽

In Vitro Experiment ◽

Pharmaceutical Protein ◽

The Stability

Background: DAB389IL-2 (Denileukin diftitox) as an immunotoxin is a targeted pharmaceutical protein and is the first immunotoxin approved by FDA. It is used for the treatment of various kinds of cancer such as CTCL lymphoma, melanoma, and Leukemia but among all of these, treatment of CTCL has special importance. DAB389IL-2 consists of two distinct parts; the catalytic domain of Diphtheria Toxin (DT) that genetically fused to the whole IL-2. Deamidation is the most important reaction for chemical instability of proteins occurs during manufacture and storage. Deamidation of asparagine residues occurs at a higher rate than glutamine residues. The structure of proteins, temperature and pH are the most important factors that influence the rate of deamidation. Methods: Since there is not any information about deamidation of DAB389IL-2, we studied in silico deamidation by Molecular Dynamic (MD) simulations using GROMACS software. The 3D model of fusion protein DAB389IL-2 was used as a template for deamidation. Then, the stability of deamidated and native form of the drug was calculated. Results: The results of MD simulations were showed that the deamidated form of DAB389IL-2 is more unstable than the normal form. Also, deamidation was carried by incubating DAB389IL-2, 0.3 mg/ml in ammonium hydrogen carbonate for 24 h at 37o C in order to in vitro experiment. Conclusion: The results of in vitro experiment were confirmed outcomes of in silico study. In silico and in vitro experiments were demonstrated that DAB389IL-2 is unstable in deamidated form.

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