Determination of yellow-green fluorescence in corn grain

2020 ◽  
Vol 29 (5) ◽  
pp. 52-53
Author(s):  
A.V. Yaitskikh ◽  
◽  
O.I. Bundina ◽  
D.S. Stepanenko ◽  
◽  
...  
Keyword(s):  
Green Fluorescence ◽  
Corn Grain

Automated Method for the Analysis of Riboflavin in Milk, with Application to Other Selected Foods

1974 ◽  
Vol 57 (5) ◽  
pp. 1085-1088
Author(s):  
James R Kirk
Keyword(s):  
Standard Deviation ◽  
Continuous Flow ◽  
Quantitative Method ◽  
Green Fluorescence ◽  
Internal Standards ◽  
Automated Method ◽  
Sodium Hydrosulfite ◽  
Automated Technique ◽  
Good Agreement

Abstract A continuous flow automated technique was developed for the determination of riboflavin in milk. The determination is based on the measurement of the natural yellow-green fluorescence of riboflavin at an excitation of 436 nm and emission of 510 nm. Blank values are determined for each sample after sodium hydrosulfite reduction of the riboflavin. Mean recovery and standard deviation for riboflavin in milk determined by the continuous flow procedure using internal standards were 9 7% and ± 2.42%, respectively. The recovery value was in good agreement with that determined using a manual procedure, while the standard deviation was 33% less than that found when using the manual procedure. The results from this study indicate that the continuous flow automated procedure for the determination of riboflavin in milk is a simple, quantitative method which eliminates many of the time-consuming analytical steps.

Automated Nitrogen Method for Feeds

1973 ◽  
Vol 56 (5) ◽  
pp. 1096-1105 ◽  
Author(s):  
Charles W Gehrke ◽  
Larry L Wall ◽  
Joseph S Absheer
Keyword(s):  
Hydrogen Peroxide ◽  
Copper Metal ◽  
Wet Digestion ◽  
Automated Method ◽  
Large Numbers ◽  
Mixed Feeds ◽  
Empirical Standard ◽  
Representative Samples ◽  
Corn Grain

Abstract A totally automated method for the determination of nitrogen in feeds has been developed. The apparatus consists of Technicon Auto-Analyzer modules. The SOLIDprep Sampler II has been interfaced with the continuous digestor to provide complete solubilization and digestion of dry samples of feed, grain, and forage materials. Hydrogen peroxide is used as an aid for the wet digestion in the continuous digestor. Mercury, selenium, or copper metal catalysts were not effective for increasing the recovery of nitrogen during the continuous digestion. Nitrogen recoveries of 88–90%, relative to ammonium sulfate, have been obtained on several materials, i.e., corn grain, wheat, barley, rice, alfalfa, fescue, mixed feeds, and feed concentrates. The recoveries from these materials average about 100±2% when an empirical standard, soy hydrolysate, is used. The system operates at 20 samples/hr and has provided precise and accurate nitrogen analyses on collaborative samples. Other laboratories cooperated with us in evaluating the method by providing representative samples and their Kjeldahl analyses. This method provides a significant reduction in the labor required for large numbers of nitrogen analyses in feeds.

scholarly journals PSVIII-5 Determination of true digestibility and the endogenous outputs of magnesium in corn for growing pigs by using the regression analysis technique

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 285-285
Author(s):  
Hongzhi Wu ◽  
Xindi Yin ◽  
Zeyu Yang ◽  
Tania Archbold ◽  
Wenyi Fan ◽  
...  
Keyword(s):  
Regression Analysis ◽  
Bone Mineralization ◽  
Block Design ◽  
Growing Pigs ◽  
Linear Relationships ◽  
Diet Formulation ◽  
Analysis Technique ◽  
Dietary Magnesium ◽  
Corn Grain

Abstract Dietary magnesium (Mg) is essential to bone mineralization. Supplemental Mg is typically not considered in commercial swine diets by assuming high bioavailability of Mg from bulky feed ingredients such as corn grain. The objectives of this study were to determine true ileal and fecal digestibility and the endogenous losses of Mg associated with corn in growing pigs by the regression analysis technique. A total of 48 barrows, with an average initial body weight (BW) 32 kg, were randomly assigned to 6 grower pig diets and were fed close to ad libitum for 10 d, with 8-d adaptation and 2-d collection fecal and the terminal ileal digesta samples, according to a randomized complete block design. Six cornstarch-based diets, containing 6 levels of Mg at 0.22, 0.32, 0.38, 0.51, 0.71 and 0.79 g/kg dry matter intake (DMI) of diets, were formulated from corn. There were linear relationships (P < 0.05), expressed as g/kg DMI, between the ileal and fecal outputs of Mg and the total intake of dietary Mg, suggesting that true ileal and fecal Mg indigestibility values (94.8±12.5 vs. 89.2±17.7%); and the ileal and fecal endogenous Mg outputs (0.16±0.02 vs. 0.21±0.11, g/kg DMI of diets) associated with corn could be estimated by the regression analysis. Our results have shown that Mg associated with conventional corn grain was very poorly digested and the gastrointestinal endogenous fecal loss of Mg was significant in the grower pig. Thus, Mg bioavailability in feeds for pigs should be assessed and supplemental of Mg may be warranted in swine diet formulation.

A spectrofluorimetric method for the determination of acitretin in pharmaceuticals

2009 ◽  
Vol 63 (4) ◽  
Author(s):  
Suling Feng ◽  
Yi Zhang ◽  
Jing Fan
Keyword(s):  
Fluorescence Spectra ◽  
Hplc Method ◽  
Relative Fluorescence Intensity ◽  
Optimum Conditions ◽  
Green Fluorescence ◽  
Spectrofluorimetric Method ◽  
Highly Sensitive ◽  
Relative Standard ◽  
Effects Of Ph

AbstractA simple, rapid, and highly sensitive spectrofluorimetric method for the determination of acitretin was developed based on the strong green fluorescence of acitretin. Influence of organic solvents on the fluorescence spectra of acitretin was studied. Effects of pH, standing time, and foreign ions on the determination of acitretin were also examined. Under the optimum conditions, linear relationship between the relative fluorescence intensity and the concentration of acitretin in the range of 30.0–1100 ng mL−1 was obtained. Detection limit of this method is 9.56 ng mL−1 for acitretin. Relative standard deviation for the determination of 480 ng mL−1 of acitretin was 1.70 %. This method was used for the determination of acitretin in pharmaceuticals and the results were compared with those obtained by the HPLC method.

scholarly journals Quality control of agricultural products (corn grain and sunflower seeds) criterion for the content of pesticide residues

2016 ◽  
pp. 278-283
Author(s):  
L.N. Chervyakova ◽  
T.P. Panchenko ◽  
N.M. Adamenko
Keyword(s):  
Quality Control ◽  
Mass Fraction ◽  
Phosphorous Acid ◽  
Agricultural Products ◽  
Photometric Method ◽  
Sunflower Seeds ◽  
Method Of Determination ◽  
Active Substances ◽  
Corn Grain

The method of determination of phosphorous acid and aluminum phosphite photometric method which allows to evaluate the mass fraction of active substances in the grain of corn and sunflower seeds, and gives the ability to control their content at the level of hygienic standards.

scholarly journals Variability Among Four Laboratories of the Filter Bag Technique to Determine Acid and Neutral Detergent Fiber Contents in Ruminant Feeds from Argentina

2009 ◽  
Vol 92 (2) ◽  
pp. 371-374
Author(s):  
José P Fay ◽  
María S Guaita ◽  
José L Danelón ◽  
Sonia Chifflet ◽  
Marisa Wawrzckiewicz ◽  
...  
Keyword(s):  
Neutral Detergent Fiber ◽  
Ring Test ◽  
Animal Science ◽  
Cornell University ◽  
Catholic University ◽  
Wide Range ◽  
Agricultural Experiment ◽  
Natural Grassland ◽  
Corn Grain

Abstract The objective of this work was to evaluate the filter bag technique (FBT) in an ANKOM 200 fiber analyzer to assess its accuracy for the determination of acid and neutral detergent fiber (ADF and NDF, respectively) contents in common ruminant feeds from the temperate region of Argentina. The evaluation was done in a ring test of 4 laboratories. The 3 Argentine laboratories were from the College of Agronomy, University of Buenos Aires, the College of Agricultural Sciences of the Catholic University of Argentina, and the Balcarce Agricultural Experiment Station of the National Institute of Agricultural Technology (INTA). The fourth laboratory was from the Department of Animal Science of Cornell University, Ithaca, NY. The feeds utilized were soybean meal expeller, whole sunflower seed, corn grain, herbages of alfalfa, ryegrass, tall wheatgrass, weeping lovegrass, guinea and barley grasses, and silages of corn and of a natural grassland. Results indicated that values obtained in the 4 laboratories were consistent regardless of the laboratory where the analyses were performed. It was concluded that the FBT in the ANKOM instrument gave satisfactory results when used for the determination of both ADF and NDF across samples having a wide range of fiber contents, and that the among-laboratories variability of this methodology was low.

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