scholarly journals Modification of fatty acid composition in soybean seeds to improve soybean oil quality and functionality

2011 ◽  
Author(s):  
◽  
Anh Tung Pham
Keyword(s):  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Content ◽  
Oil Quality ◽  
Soybean Seeds ◽  
Oleic Acid Content ◽  
High Oleic Acid ◽  
Plant Introductions ◽  
High Oleic

The purpose of this project is to modify the fatty acid composition in soybean seeds to improve soybean oil quality and functionality. By sequencing the FAD2-1A and FAD2-1B genes in 24 plant introductions, we identified two novel mutant alleles: one for each gene that is responsible for the elevated oleic acid content in four plant introductions. The combination of the newly identified mutant FAD2-1B allele with existing or the novel mutant FAD2-1A alleles created soybean lines with more than 80% oleic acid content. Combination of two mutant FAD2-1A and FAD2-1B with mutant FAD3A or mutant FAD3C or both resulted in high oleic acid content of 80 - 85% and linolenic acid content in the range from 1.5 - 4%. Perfect molecular markers associated with these mutant alleles were designed to help select the soybean lines with genotypes of interest in early generations in breeding. The high oleic acid and high oleic acid low linolenic soybeans produced have an improved stability across growing environments compared to existing sources.

scholarly journals CRISPR/Cas9-Induced fad2 and rod1 Mutations Stacked With fae1 Confer High Oleic Acid Seed Oil in Pennycress (Thlaspi arvense L.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Brice A. Jarvis ◽  
Trevor B. Romsdahl ◽  
Michaela G. McGinn ◽  
Tara J. Nazarenus ◽  
Edgar B. Cahoon ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Content ◽  
Seed Oil ◽  
Oleic Acid Content ◽  
Wild Type ◽  
High Oleic Acid ◽  
High Oleic ◽  
Thlaspi Arvense ◽  
Trade Offs

Pennycress (Thlaspi arvense L.) is being domesticated as an oilseed cash cover crop to be grown in the off-season throughout temperate regions of the world. With its diploid genome and ease of directed mutagenesis using molecular approaches, pennycress seed oil composition can be rapidly tailored for a plethora of food, feed, oleochemical and fuel uses. Here, we utilized Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 technology to produce knockout mutations in the FATTY ACID DESATURASE2 (FAD2) and REDUCED OLEATE DESATURATION1 (ROD1) genes to increase oleic acid content. High oleic acid (18:1) oil is valued for its oxidative stability that is superior to the polyunsaturated fatty acids (PUFAs) linoleic (18:2) and linolenic (18:3), and better cold flow properties than the very long chain fatty acid (VLCFA) erucic (22:1). When combined with a FATTY ACID ELONGATION1 (fae1) knockout mutation, fad2 fae1 and rod1 fae1 double mutants produced ∼90% and ∼60% oleic acid in seed oil, respectively, with PUFAs in fad2 fae1 as well as fad2 single mutants reduced to less than 5%. MALDI-MS spatial imaging analyses of phosphatidylcholine (PC) and triacylglycerol (TAG) molecular species in wild-type pennycress embryo sections from mature seeds revealed that erucic acid is highly enriched in cotyledons which serve as storage organs, suggestive of a role in providing energy for the germinating seedling. In contrast, PUFA-containing TAGs are enriched in the embryonic axis, which may be utilized for cellular membrane expansion during seed germination and seedling emergence. Under standard growth chamber conditions, rod1 fae1 plants grew like wild type whereas fad2 single and fad2 fae1 double mutant plants exhibited delayed growth and overall reduced heights and seed yields, suggesting that reducing PUFAs below a threshold in pennycress had negative physiological effects. Taken together, our results suggest that combinatorial knockout of ROD1 and FAE1 may be a viable route to commercially increase oleic acid content in pennycress seed oil whereas mutations in FAD2 will likely require at least partial function to avoid fitness trade-offs.

scholarly journals Soybean as a Model Crop to Study Plant Oil Genes: Mutations in FAD2 Gene Family

2021 ◽  
Author(s):  
Sy M. Traore ◽  
Guohao He
Keyword(s):  
Oleic Acid ◽  
Gene Family ◽  
Acid Content ◽  
Oil Quality ◽  
Model Organism ◽  
Integral Approach ◽  
Oleic Acid Content ◽  
High Oleic Acid ◽  
High Oleic ◽  
Fad2 Gene

Plants have numerous fatty acid desaturase (FAD) enzymes regulating the unsaturation of fatty acids, which are encoded by a FAD gene family. The FAD2 genes belong to such family and play a vital role in converting monounsaturated oleic acid to polyunsaturated linoleic acid. Oleic acid has the health benefits for humans, such as reduction in cholesterol level, antioxidation property, and industrial benefits like longer shelf life. The development of genotypes with high oleic acid content in seeds has become one of the primary goals in breeding oilseed plants. The identification and characterization of the FAD2 genes in plants have been an important step to better manipulate gene expression to improve the seed oil quality. The induction of mutations in FAD2 genes to reduce FAD2 enzyme activity has been an integral approach to generate genotypes with high oleic acid. This chapter will describe the FAD2 gene family in the model organism soybean and the correction of mutations in FAD2 genes with the increase of oleic acid content. Leveraging advanced research of FAD2 gene family in soybean promotes the study of FAD2 genes in other legume species, including peanut. The future perspectives and challenges associated with mutations in FAD2 genes will be discussed.

Genetic Factors Influencing High Oleic Acid Content in Spanish Market‐Type Peanut Cultivars

Crop Science ◽  
2001 ◽  
Vol 41 (1) ◽  
pp. 51-56 ◽  
Author(s):  
Yolanda López ◽  
Olin D. Smith ◽  
Scott A. Senseman ◽  
William L. Rooney
Keyword(s):  
Oleic Acid ◽  
Acid Content ◽  
Genetic Factors ◽  
Oleic Acid Content ◽  
High Oleic Acid ◽  
High Oleic ◽  
Factors Influencing ◽  
High Oleic Acid Content ◽  
Peanut Cultivars

Variability of Seed Fatty Acid Composition to Growing Degree-Days in High Oleic Acid Sunflower Genotypes

Helia ◽  
2015 ◽  
Vol 38 (62) ◽  
Author(s):  
Claudio Ferfuia ◽  
Maurizio Turi ◽  
Gian Paolo Vannozzi
Keyword(s):  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Composition ◽  
Inbred Line ◽  
Acid Content ◽  
Growing Degree Days ◽  
Environment Interaction ◽  
Oleic Acid Content ◽  
Degree Days

AbstractHigh temperature enhances the oleic acid content in the oil of normal cultivars but conflicting results are reported on temperature effects on oleic acid content in HO cultivars: either no effect or an increase in oleic acid content with temperature. To investigate the effects of temperature on HO genotypes under natural field conditions, a three-year field trial was conducted using two sowing dates and three HO genotypes (two inbred lines and one hybrid). To compare our results with previous works, growing degree-days (GDD) were computed (base temperature=6°C). GDD accumulated during the “flowering – 25 days after flowering” period influenced fatty acid composition of seed. Oleic and linoleic acid contents were affected by accumulated GDD in two HO genotypes (one inbred line and the hybrid). There was an increase of about 3% in oleic acid content as response to more high GDD accumulated. Their content was not modified by GDD in the other inbred line. There was a genotype×environment interaction that we suppose depending on modifier genes. These genetic factors affected oleic acid content. This indicated the importance of breeding targeted to select hybrids with a stable oleic acid content and higher than 90%. Saturated fatty acids (palmitic and stearic) were also influenced by temperature, and there was genetic variability among genotypes.

scholarly journals Overexpression of Soybean Transcription Factors GmDof4 and GmDof11 Significantly Increase the Oleic Acid Content in Seed of Brassica napus L.

Agronomy ◽  
2018 ◽  
Vol 8 (10) ◽  
pp. 222 ◽  
Author(s):  
Qinfu Sun ◽  
Jueyi Xue ◽  
Li Lin ◽  
Dongxiao Liu ◽  
Jian Wu ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Transcription Factors ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Brassica Napus ◽  
Acid Content ◽  
Fatty Acid Biosynthesis ◽  
Oleic Acid Content ◽  
Acid Biosynthesis ◽  
Brassica Napus L

Rapeseed (Brassica napus L.) with substantial lipid and oleic acid content is of great interest to rapeseed breeders. Overexpression of Glycine max transcription factors Dof4 and Dof11 increased lipid accumulation in Arabidopsis and microalgae, in addition to modifying the quantity of certain fatty acid components. Here, we report the involvement of GmDof4 and GmDof11 in regulating fatty acid composition in rapeseeds. Overexpression of GmDof4 and GmDof11 in rapeseed increased oleic acid content and reduced linoleic acid and linolenic acid. Both qPCR and the yeast one-hybrid assay indicated that GmDof4 activated the expression of FAB2 by directly binding to the cis-DNA element on its promoters, while GmDof11 directly inhibited the expression of FAD2. Thus, GmDof4 and GmDof11 might modify the oleic acid content in rapeseed by directly regulating the genes that are associated with fatty acid biosynthesis.

scholarly journals Agronomic Traits and Fatty Acid Composition of High‑Oleic Acid Cultivar Hosim

2018 ◽  
Vol 6 (1) ◽  
pp. 44-50 ◽  
Author(s):  
Jeong-Dong Lee ◽  
Minsu Kim ◽  
Krishnanand P. Kulkarni ◽  
Jong Tae Song
Keyword(s):  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Composition ◽  
Agronomic Traits ◽  
High Oleic Acid ◽  
High Oleic

scholarly journals IDENTIFICATION OF HIGH SEED OIL YIELD AND HIGH OLEIC ACID CONTENT IN BRAZILIAN GERMPLASM OF WINTER SQUASH (Cucurbita moschata D.)

2021 ◽  
Author(s):  
Ronaldo Silva Gomes ◽  
Ronaldo Machado Júnior ◽  
Cleverson Freitas de Almeida ◽  
Rebeca Lourenço de Oliveira ◽  
Rafael Ravaneli Chagas ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Acid Content ◽  
Seed Oil ◽  
Linoleic Acid Content ◽  
Oleic Acid Content ◽  
Cucurbita Moschata ◽  
High Oleic Acid ◽  
Genetic Breeding ◽  
High Oleic ◽  
High Oleic Acid Content

Cucurbita moschata D. seed oil contains approximately 75% unsaturated fatty acids, with high levels of monounsaturated fatty acids and antioxidant compounds such as vitamin E and carotenoid, constituting a promising food in nutritional terms. Associated to this, the Brazilian germplasm of C. moschata exhibits remarkable variability, representing an important source for the genetic breeding of this vegetable and other cucurbits. In this context, the present study evaluated the productivity and profile of the seed oil of 91 C. moschata accessions from different regions of Brazil and maintained in the Vegetable Germplasm Bank of the Federal University of Viçosa (BGH-UFV). A field experiment was conducted between January and July 2016. The tested C. moschata accessions showed high genetic variability in terms of characteristics related to seed oil productivity (SOP), such as the mass of seeds per fruit and productivity of seeds, providing predicted selection gains of 29.39 g and 0.26 t ha -1 , respectively. Based on the phenotypic and genotypic correlations, greater SOP can be achieved while maintaining high oleic acid content and low linoleic acid content, providing oil of better nutritional and chemical quality. In variability analysis, the accessions were clustered into five groups, which presented different averages for SOP and fatty acid content of seed oil; approach that will guide the use of appropriate germplasm in programs aimed at genetic breeding for SOP and seed oil profile. Per se analysis identified BGH-4610, BGH-5485A, BGH-6590, BGH-5556A, BGH-5472A, and BGH-5544A as the most promising accessions in terms of SOP, with average (m+g) of approximately 0.20 t ha -1 . The most promising accessions for higher oleic acid content of seed oil were BGH-5456A, BGH-3333A, BGH-5361A, BGH-5472A, BGH-5544A, BGH-5453A, and BGH-1749, with average (m+g) of approximately 30%, and almost all of these accessions were also the most promising in terms of lower linoleic acid content of seed oil, with average (m+g) of approximately 45%. Overall, part of the C. moschata accessions evaluated in the present study can serve as a promising resource in genetic breeding programs for SOP and fatty acid profile, aiming at the production of oil with better nutritional and physicochemical quality.

scholarly journals ‘Zeitoun Ennour’: A new olive (Olea europaea L.) cultivar in Tunisia with high oil quality

2021 ◽  
Vol 2 (4) ◽  
pp. 1-6
Author(s):  
Fathi Ben Amar ◽  
Imen Guellaoui ◽  
Mohamed Ayadi ◽  
Olfa Elloumi ◽  
Mohamed Ali Triki ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Oil Quality ◽  
Oleic Acid Content ◽  
Dual Purpose ◽  
High Oleic ◽  
Local Cultivar ◽  
Self Compatibility ◽  
Late Maturity

An olive breeding program was started in Tunisia in 1993 in order mainly to improve the fatty acid composition of the local cultivar ‘Chemlali Sfax’. ‘Zeitoun Ennour’ is a new cultivar obtained from a cross between ‘Chemlali Sfax’ and the local dual-purpose use cultivar ‘Chemchali Gafsa’. The morphological study of this cultivar showed that eleven characters dealing with fruit and endocarp differed from ‘Chemlali Sfax’, mainly regarding to their respective weights. This new cultivar had the same sensitivity to Verticillium dahliae Kleb and earlier bearing than the original variety. Its olive production was considered as high as for ‘Chemlali Sfax’ but with partial self-compatibility and late maturity. The new cultivar realized a net improvement in comparison with the original cultivar particularly regarding its fatty acid composition with very high oleic acid content (>75 %) and low palmitic and linoleic acid contents (<10 %). The new cultivar was recently released and will be available for growers as soon as possible.

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