Variability of Seed Fatty Acid Composition to Growing Degree-Days in High Oleic Acid Sunflower Genotypes

Helia ◽  
2015 ◽  
Vol 38 (62) ◽  
Author(s):  
Claudio Ferfuia ◽  
Maurizio Turi ◽  
Gian Paolo Vannozzi
Keyword(s):  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Composition ◽  
Inbred Line ◽  
Acid Content ◽  
Growing Degree Days ◽  
Environment Interaction ◽  
Oleic Acid Content ◽  
Degree Days

AbstractHigh temperature enhances the oleic acid content in the oil of normal cultivars but conflicting results are reported on temperature effects on oleic acid content in HO cultivars: either no effect or an increase in oleic acid content with temperature. To investigate the effects of temperature on HO genotypes under natural field conditions, a three-year field trial was conducted using two sowing dates and three HO genotypes (two inbred lines and one hybrid). To compare our results with previous works, growing degree-days (GDD) were computed (base temperature=6°C). GDD accumulated during the “flowering – 25 days after flowering” period influenced fatty acid composition of seed. Oleic and linoleic acid contents were affected by accumulated GDD in two HO genotypes (one inbred line and the hybrid). There was an increase of about 3% in oleic acid content as response to more high GDD accumulated. Their content was not modified by GDD in the other inbred line. There was a genotype×environment interaction that we suppose depending on modifier genes. These genetic factors affected oleic acid content. This indicated the importance of breeding targeted to select hybrids with a stable oleic acid content and higher than 90%. Saturated fatty acids (palmitic and stearic) were also influenced by temperature, and there was genetic variability among genotypes.

scholarly journals THE INFLUENCE OF YIELD ON FATTY ACID COMPOSITION OF PECAN NUTS

HortScience ◽  
1995 ◽  
Vol 30 (3) ◽  
pp. 432d-432
Author(s):  
J.B. Storey ◽  
L.J. Grauke ◽  
Laurence Sistrunk ◽  
Tommy E. Thompson
Keyword(s):  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Acid Composition ◽  
Acid Content ◽  
Linoleic Acid Content ◽  
Oleic Acid Content ◽  
High Quality ◽  
Kernel Color

Four cultivars of pecan [Carya illinoinensis (Wangenh.) K. Koch] were studied for 3 years to determine if variations in yield influence fatty acid composition of kernels. Trees used in the study are part of the U.S. Dept. of Agriculture, Agricultural Research Service Historical Block, a test orchard planted in randomized block design with four blocks, having one single-tree replication per block and containing 36 cultivars. Four trees of each of four cultivars (`Cheyenne', `Mohawk', `Pawnee', and `Osage') were used in this test. Trees were in their 5th to 7th leaf from grafting and showed patterns of increasing yield over time for each cultivar. `Osage' was earliest to mature nuts each year and produced nuts with the lowest linoleic acid content. `Cheyenne' was latest to mature nuts and had nuts with the highest linoleic acid content. Oleic acid composition varied with yield in `Osage' and `Pawnee': as yield (kilogram/square decimeter trunk area) increased, oleic acid content decreased. Kernel color, as determined by a Hunter LabScan 5100 Spectrocolorimeter, varied in relation to fatty acid composition for `Osage' and `Pawnee': as oleic acid content increased, kernel lightness decreased. High oleic acid content and light kernel color are associated with high-quality pecans. The pattern of decreasing oleic acid content associated with increasing kernel lightness raises questions concerning the role kernel color evaluation should play in selecting high-quality pecan cultivars.

scholarly journals Overexpression of Soybean Transcription Factors GmDof4 and GmDof11 Significantly Increase the Oleic Acid Content in Seed of Brassica napus L.

Agronomy ◽  
2018 ◽  
Vol 8 (10) ◽  
pp. 222 ◽  
Author(s):  
Qinfu Sun ◽  
Jueyi Xue ◽  
Li Lin ◽  
Dongxiao Liu ◽  
Jian Wu ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Transcription Factors ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Brassica Napus ◽  
Acid Content ◽  
Fatty Acid Biosynthesis ◽  
Oleic Acid Content ◽  
Acid Biosynthesis ◽  
Brassica Napus L

Rapeseed (Brassica napus L.) with substantial lipid and oleic acid content is of great interest to rapeseed breeders. Overexpression of Glycine max transcription factors Dof4 and Dof11 increased lipid accumulation in Arabidopsis and microalgae, in addition to modifying the quantity of certain fatty acid components. Here, we report the involvement of GmDof4 and GmDof11 in regulating fatty acid composition in rapeseeds. Overexpression of GmDof4 and GmDof11 in rapeseed increased oleic acid content and reduced linoleic acid and linolenic acid. Both qPCR and the yeast one-hybrid assay indicated that GmDof4 activated the expression of FAB2 by directly binding to the cis-DNA element on its promoters, while GmDof11 directly inhibited the expression of FAD2. Thus, GmDof4 and GmDof11 might modify the oleic acid content in rapeseed by directly regulating the genes that are associated with fatty acid biosynthesis.

GLC analysis of Indian rapeseed-mustard to study the variability of fatty acid composition

2000 ◽  
Vol 28 (6) ◽  
pp. 581-582 ◽  
Author(s):  
N. Kaushik ◽  
A. Agnihotri
Keyword(s):  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Erucic Acid ◽  
Linolenic Acid ◽  
Acid Composition ◽  
Acid Content ◽  
Erucic Acid Content ◽  
High Erucic Acid ◽  
Low Erucic Acid

Rapeseed-mustard is one of the most economically important oilseed crops in India. Speciality oils having high amounts of a specific fatty acid are of immense importance for both nutritional and industrial purposes. Oil high in oleic acid has demand in commercial food-service applications due to a long shelf-life and cholesterol-reducing properties. Both linoleic and linolenic acids are essential fatty acids; however, less than 3% linolenic acid is preferred for oil stability. High erucic acid content is beneficial for the polymer industry, whereas low erucic acid is recommended for food purposes. Therefore, it is important to undertake systematic characterization of the available gene pool for its variable fatty acid profile to be utilized for specific purposes. In the present study the Indian rapeseed-mustard germplasm and some newly developed low-erucic-acid strains were analysed by GLC to study the fatty acid composition in these lines. The GLC analysis revealed that the rapeseed-mustard varieties being commonly grown in India are characterized by high erucic acid content (30–51%) in the oil with low levels of oleic acid (13–23%). However, from among the recently developed low-erucic-acid strains, several lines were identified with comparatively high oleic acid (60–70%), moderate to high linoleic acid (13–40%) and low linolenic acid (< 10%) contents. Work is in progress at TERI (New Delhi, India) to utilize these lines for development of strains with particular fatty acid compositions for specific purposes.

scholarly journals Influence of different irrigation and nitrogen levels on crude oil and fatty acid composition of maize (Zea mays L.)

2017 ◽  
Vol 68 (3) ◽  
pp. 207 ◽  
Author(s):  
M. Kaplan ◽  
H. Kale ◽  
K. Karaman ◽  
A. Unlukara
Keyword(s):  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Crude Oil ◽  
Acid Composition ◽  
Acid Content ◽  
Field Capacity ◽  
Fertilizer Treatment ◽  
Treatment Combination ◽  
Abundant Fatty Acid

The effect of irrigation and nitrogen fertilizer levels on the crude oil and fatty acid composition of maize cultivars was studied. Three levels of irrigation (50, 75 and 100% of field capacity) and nitrogen (100, 200 and 300 kg·ha-1) were used for treatment groups. After harvest, the crude oils were extracted and fatty acid profiles were determined by Gas Chromatography system. The study was repeated for two years and the interaction effects of fertilizer and irrigation were determined. Our results show that the crude oil content was affected positively by the fertilizer and the irrigation applications. As expected, the most abundant fatty acid was linoleic and the harvest year did not alter it. The highest linoleic acid content value was obtained with a 50% field capacity and 300 kg·ha-1 fertilizer treatment combination. In addition, fatty acid contents varied with the changing of interaction effects except for myristic and palmitic acid. Oleic acid was the second abundant fatty acid in the oil samples and the lowest oleic acid value was obtained with a 50% field capacity and 300 kg·ha-1 fertilizer treatment combination. Oleic acid content tended to increase with 75% field capacity but 100% field capacity treatment decreased in it.

scholarly journals Time-course effects of protein malnutrition on hepatic fatty acids Δ6 and Δ5 desaturation in the growing rat

1988 ◽  
Vol 60 (2) ◽  
pp. 389-402 ◽  
Author(s):  
Michel Narce ◽  
Jean-Pierre Poisson ◽  
Jacques Belleville ◽  
Bernard Chanussot
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Composition ◽  
Time Course ◽  
Protein Diet ◽  
Oleic Acid Content ◽  
Total Lipids ◽  
Δ5 Desaturase

1. In growing rats, the time-course effects of giving a normal-protein diet (200 g casein/kg; NP) for 52 d, a low-protein diet (20 g casein/kg; LP) for 52 d and a LP diet for 26 d followed by balanced refeeding (200 g casein/kg; BR) for 26 d, on the fatty acid composition of liver total lipids and microsomal phospholipids were investigated together with Δ6- and Δ5-microsomal desaturase activities.2. The oleic acid content (mg/g tissue) of liver total lipids increased progressively with the LP diet, while linoleic acid was increased only at days 7 and 52. 20:3ω6, 20:4ω6, 22:5ω6 and 22:6ω3 fatty acids decreased during the period on the LP diet. BR for 7 d was sufficient to restore the fatty acid composition of total lipids to control values. Changes in the fatty acid composition of liver microsomal L-α-phosphatidylcholines were observed only after 52 d on the LP diet; the proportions (% w/w total fatty acids) of 18:0, 20:3ω6 and 20:4ω6 fatty acids decreased while oleic acid increased. The fatty acid composition of L-α-phosphatidylethanolamines was less affected.3. Δ6- and Δ5-desaturase activities decreased to 20–30% of their original values after 2 d on the LP diet; a smaller deficit prevailed after 14 d but disappeared after 25 d, to appear again after 52 d. As early as day 2 of BR, desaturase activities were greatly recovered and returned to control values at day 13.4. The present work shows that modifications in microsomal Δ6- and Δ5-desaturase activities are not strictly paralleled by the changes in the composition of fatty acids of liver total lipids and microsomal phospholipids.

scholarly journals Variability of Essential and Nonessential Fatty Acids of Irish Rapeseed Oils as an Indicator of Nutritional Quality

2022 ◽  
Vol 2022 ◽  
pp. 1-10
Author(s):  
Rebecca Coughlan ◽  
Siobhan Moane ◽  
Tracey Larkin
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Acid Composition ◽  
Acid Content ◽  
Fatty Acid Content ◽  
The Third ◽  
Saturated Fatty Acid Content

The low saturated fatty acid content of rapeseed oil has resulted in it being classed as one of the most health-benefiting culinary oils. This study determines whether Irish rapeseed oils contain identical fatty acid profiles or whether distinct profiles exist between producers and producers’ successive oil batches. The fatty acid content of Irish rapeseed oils was determined in terms of the desirable MUFA and PUFA and saturated content of these oils. The fatty acid composition demonstrated significant differences in individual unsaturated fatty acid content, while total saturation had insignificant differences. Saturated fatty acid content ranged from 6.10 to 15.8%, while unsaturated fatty acids ranged from 84.20 to 90.10%. Moreover, individual fatty acid content exhibited significant differences ( p < 0.05 ). Oleic acid (C18:1), linoleic acid (C18:2), and stearic acid (C18:0) contents were considered significantly different from other fatty acids detected. The third successive batch from each producer exhibited lower oleic acid content, and the third batch contained higher linoleic acid content, at the same time maintaining a desirable unsaturated fatty acid composition. Studies suggest that differences in the fatty acid composition may be due to cultivation practices such as climate, soil composition, sowing and harvesting, processing techniques, and oxidation reactions.

scholarly journals Heritability of oleic acid content in sunflower seed oil of recombinant inbred lines

2020 ◽  
Vol 183 (3) ◽  
pp. 27-30
Author(s):  
Ya.N. Demurin ◽  
◽  
Yu.V. Chebanova ◽  
O.M. Borisenko ◽  
T.A. Kovalenko ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Recombinant Inbred ◽  
Acid Content ◽  
Sunflower Seed ◽  
Seed Oil ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Oleic Acid Content

The study of the heritability of the oleic acid content in seed oil in recombinant inbred lines is the genetic basis for effective breeding work on the quality of sunflower oil. The experiments were carried out under field and laboratory conditions in VNIIMK, Krasnodar, Russian Federation in 2016-2020. We used 17 recombinant inbred sunflower lines of I4 and I5 generations obtained from crossing a medium-oleic LG27 line and a high-oleic LG26 line with subsequent self-pollination. The fatty acid composition of sunflower seed oil was analyzed using the method of gas-liquid chromatography of methyl esters on the Chromatek-Kristall 5000 device. Seventeen recombinant inbred sunflower lines in generation I4 showed a wide variation in the content of oleic acid in the oil of average seed samples from 39.00 % (RIL-1) to 92.24 % (RIL-42) and linoleic acid – from 43.28 to 1.35 %, respectively. In 2016, three lines were characterized 28 by an average oleic acid content of 55.64-65.54 %. Analysis of the fatty acid composition of oil in individual seeds of the next generation I5 of these lines confirmed, in general, their phenotypic ranks with a range of variability from 32.18 to 92.15 % in the content of oleic acid. The middle oleic lines RIL-21, RIL29 and RIL-30 also showed belonging to their phenotypic class in 2019 in the range of values from 59.80 to 63.14 %. The study of the conjugate variability of oleic acid values in the parent-progeny series in generations of I4–I5 revealed the presence of a significant strong positive correlation r = 0.97. At the same time, the coefficient of determination, defined as the square of the correlation coefficient which evaluates the degree of heritability of a trait, was 0.95, which indicates a significant influence of the genotype factor in general phenotypic variation.

scholarly journals Modification of fatty acid composition in soybean seeds to improve soybean oil quality and functionality

2011 ◽  
Author(s):  
◽  
Anh Tung Pham
Keyword(s):  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Content ◽  
Oil Quality ◽  
Soybean Seeds ◽  
Oleic Acid Content ◽  
High Oleic Acid ◽  
Plant Introductions ◽  
High Oleic

The purpose of this project is to modify the fatty acid composition in soybean seeds to improve soybean oil quality and functionality. By sequencing the FAD2-1A and FAD2-1B genes in 24 plant introductions, we identified two novel mutant alleles: one for each gene that is responsible for the elevated oleic acid content in four plant introductions. The combination of the newly identified mutant FAD2-1B allele with existing or the novel mutant FAD2-1A alleles created soybean lines with more than 80% oleic acid content. Combination of two mutant FAD2-1A and FAD2-1B with mutant FAD3A or mutant FAD3C or both resulted in high oleic acid content of 80 - 85% and linolenic acid content in the range from 1.5 - 4%. Perfect molecular markers associated with these mutant alleles were designed to help select the soybean lines with genotypes of interest in early generations in breeding. The high oleic acid and high oleic acid low linolenic soybeans produced have an improved stability across growing environments compared to existing sources.

scholarly journals Cholesterol Content, Fatty Acid Composition and Sensory Analysis of Deep Fried and Roasted Abon Ikan (Fish Floss/Shredded Fish Flesh)

2020 ◽  
Vol 147 ◽  
pp. 03009
Author(s):  
A Suhaeli Fahmi ◽  
Lukita Purnamayati
Keyword(s):  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Palmitic Acid ◽  
Acid Composition ◽  
Sensory Analysis ◽  
Cholesterol Content ◽  
Deep Frying ◽  
Oleic Acid Content

Abon ikan (fish floss/shredded fish) commonly processed by deep frying in cooking oil after fish meat were steamed and mixed with condiments. Deep frying technique used in abon ikan processing caused high rancidity risk of abon ikan during storage. In this research, deep frying and roasting method were compared. Fresh catfish (Clarias gariepinus) were used as raw material, after steamed and mixed with condiments, then mixed dough were processed with the treatments (roasted on pan or deep fried with frying oil). Cholesterol content was tested by Bohac test. Fatty acid composition was analyzed with Gas Chromatography. Roasted abon ikan contained moisture, fat and protein about 9.94%, 22.39% and 29.66% respectively while fried abon ikan contained about 4.98%, 23.19% and 27.50% respectively. Roasted abon ikan contained cholesterol about 0.28 mg/g and fried abon ikan contained about 0.74 mg/g. Fatty acid profile analysis show that in both samples unsaturated fatty acid were dominated by oleic acid and linoleic acid while saturated fatty acid were dominated by palmitic acid. Roasted abon ikan were lower in palmitic acid and oleic acid content but higher in palmitoleic acid and linoleic acid. Sensory analysis showed that roasted abon ikan gave better texture, flavor and odor while deep fried abon ikan was better in appearance.

scholarly journals TILLING-by-Sequencing+ Reveals the Role of Novel Fatty Acid Desaturases (GmFAD2-2s) in Increasing Soybean Seed Oleic Acid Content

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1245
Author(s):  
Naoufal Lakhssassi ◽  
Valéria Stefania Lopes-Caitar ◽  
Dounya Knizia ◽  
Mallory A. Cullen ◽  
Oussama Badad ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Content ◽  
Edible Oils ◽  
Fatty Acid Desaturase ◽  
Soybean Seed ◽  
Oleic Acid Content ◽  
Fatty Acid Production ◽  
Tilling By Sequencing

Soybean is the second largest source of oil worldwide. Developing soybean varieties with high levels of oleic acid is a primary goal of the soybean breeders and industry. Edible oils containing high level of oleic acid and low level of linoleic acid are considered with higher oxidative stability and can be used as a natural antioxidant in food stability. All developed high oleic acid soybeans carry two alleles; GmFAD2-1A and GmFAD2-1B. However, when planted in cold soil, a possible reduction in seed germination was reported when high seed oleic acid derived from GmFAD2-1 alleles were used. Besides the soybean fatty acid desaturase (GmFAD2-1) subfamily, the GmFAD2-2 subfamily is composed of five members, including GmFAD2-2A, GmFAD2-2B, GmFAD2-2C, GmFAD2-2D, and GmFAD2-2E. Segmental duplication of GmFAD2-1A/GmFAD2-1B, GmFAD2-2A/GmFAD2-2C, GmFAD2-2A/GmFAD2-2D, and GmFAD2-2D/GmFAD2-2C have occurred about 10.65, 27.04, 100.81, and 106.55 Mya, respectively. Using TILLING-by-Sequencing+ technology, we successfully identified 12, 8, 10, 9, and 19 EMS mutants at the GmFAD2-2A, GmFAD2-2B, GmFAD2-2C, GmFAD2-2D, and GmFAD2-2E genes, respectively. Functional analyses of newly identified mutants revealed unprecedented role of the five GmFAD2-2A, GmFAD2-2B, GmFAD2-2C, GmFAD2-2D, and GmFAD2-2E members in controlling the seed oleic acid content. Most importantly, unlike GmFAD2-1 members, subcellular localization revealed that members of the GmFAD2-2 subfamily showed a cytoplasmic localization, which may suggest the presence of an alternative fatty acid desaturase pathway in soybean for converting oleic acid content without substantially altering the traditional plastidial/ER fatty acid production.

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