scholarly journals Microwave-assisted extraction of Taxol and 10-Deacetyl baccatin III from the leaves and branches of red pine (Taxus wallichiana Zucc.)

2016 ◽  
Vol 19 (3) ◽  
pp. 76-87
Author(s):  
Phung Thi Kim Le ◽  
Quan Thi Hong Vu ◽  
Tuyet Thi Ngoc Nguyen ◽  
Khoa Anh Tran ◽  
Kien Anh Le
Keyword(s):  
Chloroform Extract ◽  
Red Pine ◽  
Solvent Ratio ◽  
Microwave Assisted Extraction ◽  
Baccatin Iii ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction ◽  
Short Time

Taxol has been by far the most well-known worldwide as an effective anticancer natural drug. With the great treatment abilities at low concentration, Taxol is a considerable interest of many scientists in various fields. The commercial products of Taxol can be isolated directly from Taxus species or can be synthesized from 10-deacetylbaccatin III (10-DAB III) or baccatin III (BC III), which are known as precursors of Taxol, by using semisynthetic methods. In this study, the extraction of Taxol and 10-DAB III from the leaves and branches of red pine cultivated in Lam Dong Province, Vietnam was carried out. Some traditional methods such as Soxhlet, maceration as well as the modern methods such as microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE) were used to extract and evaluate the extraction efficiency. The concentration of 10-DAB III experienced at over 90% as compared to maceration and the amount of Taxol accounted for approximately 80% of Soxhlet. MAE was thus more suitable for recovering both Taxol and 10-DAB III than the others because of its short time and less solvent consumption. Besides, four parameters including solvent nature (MeOH and EtOH), extraction time, material/solvent ratio (1:10, 1:15, 1:20 and 1:25) and microwave power (40W, 240W and 440W) were investigated the effects of these elements on the content of 10-DAB III and Taxol. The results illustrated that the optimal conditions providing 95.85% 10-DAB III were as follows: 240W, 1:15 ratio and 20 minutes for extraction. To get the highest amount of Taxol (79.83%), extraction was subjected at these conditions concluding 40W, 1:25 ratio and 20 minutes. Diaion, NP-silicagel were used to enrich taxol and 10-DAB III from methanol extract and chloroform extract, respectively. The highest concentration of Taxol and 10-DAB III constituted at 0.64% and 19.76% with NP-silicagel (PE: aceton, 7:3).

scholarly journals EKSTRAKSI FLAVONOID DARI DAUN KERSEN (Muntinga calabura L) MENGGUNAKAN PELARUT ETANOL DENGAN METODE MAE (Microwave Assisted Extraction) DAN UAE (Ultrasonic Assisted Extraction)

2021 ◽  
Vol 6 (2) ◽  
pp. 105
Author(s):  
Siti Indana Isdiyanti ◽  
Laeli Kurniasari ◽  
Farikha Maharani
Keyword(s):  
Solvent Ratio ◽  
Maximum Point ◽  
Microwave Assisted Extraction ◽  
Flavonoid Content ◽  
Ultrasound Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction ◽  
Ultrasound Assisted

Kersen (Muntinga calabura L) adalah pohon yang memiliki buah kecil dan manis. Tumbuhan ini dimanfaatkan antara lain sebagai  obat penurun panas, mengobati pembengkakan kelenjar prostat dan mengobati penyakit asam urat, selain itu juga dapat dimanfaatkan sebagai antiseptic, antioksidan, antimikroba, dan anti inflamasi. Berbagai macam metode ekstraksi telah dikembangkan dari yang konvensional ke metode modern, dimana salah satunya yaitu Microwave Assisted Extraction (MAE) dan Ultrasound Assisted Extraction (UAE). Penelitian ini bertujuan untuk menganalisa pengaruh metode ekstraksi yang dilakukan terhadap rendemen, menganalisa pengaruh rasio solid berbanding liquid serta waktu terhadap rendemen, dan menguji kadar flavonoid yang dihasilkan dari kedua metode tersebut. Percobaan dilakukan dengan variabel rasio-pelarut 1:5 – 1:25, sedangkan variabel waktu 5 menit – 25 menit. Hasil percobaan menunjukan bahwa ke dua variabel berpengaruh terhadap konsentrasi flavonoid hasil ekstraksi. Secara umum meningkat seiring kenaikan variabel sampai maksimum di titik tertentu, kemudian turun. Konsentrasi maksimum diperoleh pada variabel umpan-pelarut MAE sebesar 1:25 dengan kadar flavonoid 132,41 mg/ml dan rendemen sebesar 39%. Adapun waktu terbaik adalah 5 menit dengan kadar flavonoid 91,669 mg/ml dan rendemen sebesar 22,7%. Sedangkan pada proses UAE (Ultrasonic Assisted Extraction) dengan rasio umpan-pelarut maksimal sebesar 1:10 dengan kadar flavonoid 47,5899 mg/ml dan rendemen 26%. Sedangkan waktu terbaik  10 menit dengan kadar flavonoid 56,7769 mg/ml dan rendemen sebesar 19,65%. AbstracKersen  (Muntinga calabura L) is a tree with small and sweet fruit. The fruit is used, among others, as a febrifuge, treating swelling of the prostate gland and treating gout. Besides the fruit, its leaves also has potential as an antiseptic, antioxidant, antimicrobial, and anti-inflammatory. Various extraction methods have been developed from conventional to modern methods, one of which is Microwave Assisted Extraction (MAE) and Ultrasound Assisted Extraction (UAE). The research will extract the kersen leaves and aim to analyze the effect of the extraction method on the yield, the effect of the solid to liquid ratio and time on the yield, and the flavonoid content produced by both methods. The experiment was carried out with a solvent-ratio variable of 1:5 – 1:25, while the time variable was 5 – 25 minutes. The results of the experiment showed that the two variables had an effect on the concentration of flavonoids extracted. In general, it increases as the variables increase to a maximum point, then decreases. The maximum concentration obtained in the feed-solvent variable MAE was 1:25 with a flavonoid content of 132.41 mg/ml, with a yield of 39% and the best time was 5 minutes with a flavonoid content of 91.669 mg/ml, with a yield of 22.7%. While in the UAE Ultrasonic Assisted Extraction process with a maximum feed-solvent ratio of 1:10 with a flavonoid content 47.5899 mg/ml and yield  26%, while the best time of process was 10 minutes with  flavonoid content 56.7769 mg/ml and yield 19, 65%.Kata kunci: Flavonoid, Ekstrak Kersen, MAE, UA

scholarly journals Extraction Optimization of 5,7-Dihydroxy-6,8,4′-trimethoxyflavonol, a Bioactive Flavonoid from Rubus rosifolius (Rosaceae) Leaves

2019 ◽  
Vol 14 (1) ◽  
pp. 1934578X1901400
Author(s):  
Janieire Lorraine da Rocha Pittarello ◽  
Marcel Petreanu ◽  
Valdir Cechinel Filho ◽  
Clóvis Antonio Rodrigues ◽  
Luiz Carlos Klein-Júnior ◽  
...  
Keyword(s):  
Extraction Method ◽  
High Performance ◽  
Solvent Ratio ◽  
Flavonoid Content ◽  
Box Behnken Design ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction ◽  
Addition Level

Recently, it has been demonstrated that a rare flavonoid, 5,7-dihydroxy-6,8,4′-trimethoxyflavonol (1), isolated from the leaves of Rubus rosifolius showed significant antiproliferative activity against different cancer cell lines and had a diuretic effect. This study aimed to determine the best extraction method evaluating dynamic maceration extraction (DME), ultrasonic assisted extraction (UAE), and microwave assisted extraction (MAE). In addition, level optimization for different factors was focused using Design of Experiments. The flavonoid content was estimated by high performance liquid chromatography (HPLC). The results indicated that UAE provided the highest content with the shortest extraction time. For optimization, response surface methodology (RSM) was used, applying a Box-Behnken Design (BBD) with three factors at three levels. It was possible to observe that the flavonoid content significantly increased with the increase of temperature (max. 70°C). The time had little influence on the extraction and, for this reason, was set at 25 min. With regard to the drug/solvent ratio, it was observed that this had an impact on yield with a ratio of 3.5%. A model was built and a determination coefficient of 0.98 was obtained. After validation, an optimized method was developed, able to extract 1 with a lower time, lower solvent consumption, and affording higher contents.

scholarly journals Ekstraksi Polifenol Total dari Daun Kadamba (Mitragyna speciosa Korth) Menggunakan Metode Choline Chloride-Sorbitol based Microwave Assisted Extraction

2019 ◽  
Vol 10 ◽  
pp. 131-134
Author(s):  
Ramila Hidayati ◽  
Dewi Rahmawati ◽  
Islamudin Ahmad
Keyword(s):  
Choline Chloride ◽  
Solvent Ratio ◽  
Microwave Assisted Extraction ◽  
Green Solvent ◽  
Microwave Assisted ◽  
Extract Solution ◽  
Mitragyna Speciosa ◽  
Assisted Extraction ◽  
Total Extraction

This research was conducted to determine the influence of the use of the choline chloride-sorbitol based microwave assisted extraction (MAE) method against the total extraction of polyphenols from the leaf Kadamba (Mitragyna speciosa Korth). Simplisia extracted using solvent (green solvent) choline chloride-sorbitol with a ratio of 1:1; 1:2 and 1:3 g/g with sample and solvent ratio of 1:10 g/mL, microwave strength is 50% watt and performed for 10 minutes, the extract solution has been obtained and then done determination of total levels of polyphenols by using folin ciocalteu reagents and further measured its absorption with the UV-VIS spectrophotometer device at a wavelength of 770 nm. Based on the results of the study, obtained the levels of polyphenols from each comparison of solvent used against the sample of 334 mg of GAE/g samples (1:1 g/g solvent); 372 mg GAE/g samples (1:2 g/g solvents) and 299 mg of GAE/g samples (1:3 g/g solvents). Based on the results of the study can be concluded that the method of choline chloride-sorbitol based microwave assisted extraction (MAE) can be used to extract the polyphenols from Kadamba leaves easily, quickly and efficiently.

scholarly journals Optimisation of microwave-assisted extraction of flavonoids and phenolics from celery (Apium graveolens L.) leaves by response surface methodology

2016 ◽  
Vol 34 (No. 4) ◽  
pp. 341-349 ◽  
Author(s):  
Q. He ◽  
Y. Li ◽  
P. Zhang ◽  
A. Zhang ◽  
H. Wu
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Apium Graveolens ◽  
Solvent Ratio ◽  
Microwave Assisted Extraction ◽  
Inhibition Rate ◽  
Microwave Assisted ◽  
Assisted Extraction

The conditions for microwave-assisted extraction (MAE) of total flavonoids (TF) and total phenols (TP), and antioxidant activity from celery (Apium graveolens L.) leaves by response surface methodology (RSM) were optimised. The 3-level, 3-factorial Box-Behnken design (BBD) was employed to study three main extraction conditions: microwave power (300–500 W), solid-solvent ratio (15–30 ml/g), and ethanol concentration (50–80%). It was found that microwave power of 500 W at 30 ml/g solid-solvent ratio with 75.6% (v/v) ethanol concentration was the most optimum conditions for the extraction of TF and TP from celery leaves with the consequent high antioxidant activity measured by the DPPH inhibition rate. Using the optimum extraction conditions, the extraction yields of TF and TP were 0.62 g RUE/100 g DW, 3.01 g GAE/100 g DW, respectively, and the DPPH inhibition rate was 88%. The results indicated that the nutritional quality of celery (Apium graveolens L.) leaves could be improved significantly by optimising the extraction process of MAE using response surface methodology.

scholarly journals EKSTRAKSI TANIN DARI KULIT KAYU AKASIA DENGAN MENGGUNAKAN MICROWAVE: PENGARUH DAYA MICROWAVE, WAKTU EKSTRAKSI DAN JENIS PELARUT

2017 ◽  
Vol 6 (3) ◽  
pp. 52-57
Author(s):  
Iriany ◽  
Florentina Pandiangan ◽  
Christina Eka P
Keyword(s):  
Atomic Absorption ◽  
Adsorption Capacity ◽  
Microwave Power ◽  
Polar Solvent ◽  
Extraction Time ◽  
Solvent Ratio ◽  
Microwave Assisted Extraction ◽  
Power Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction

Tannin is one of complex polyphenol compound which soluble in polar solvent. Tannin could be extracted from acacia bark. This study aims to examine microwave-assisted extraction of tannins under influence of microwave power, extraction time, and solvent and its application to adsorb Cd and Cu. Tannin was extracted from acacia bark with feed-to-solvent ratio (1:20 g/ml). Power of microwave was adjusted 100 W, 180 W, 300 W, 450 W, and 600 W for 1 minute, 3 minutes and 5 minutes respectively using aquadest and ethanol as the solvent. The extracts were analyzed using UV-Vis spectrophotometer. Furthermore, the extract with the highest yield of tannins are used as the adsorbent. The concentration of Cd and cu were analyzed using Atomic Absorption Spectrophotometer. The result showed the highest yield of tannin 26.606 mg/g at 100 W and 3 minutes extraction time using ethanol as the solvent. The result showed that the adsorption capacity of adsorbent from tannin was 3.81 mg/g and 2.26 mg/g for Cd and Cu respectively.

scholarly journals Optimization of Anthocyanin Extraction from Cockspur Coral (Erythrina Crista-Galli L.) Petals with Microwave-Assisted Extraction (MAE) using Response Surface Methodology

2021 ◽  
Vol 21 (2) ◽  
pp. 143
Author(s):  
Astrilia Damayanti ◽  
Bayu Triwibowo ◽  
Megawati Megawati ◽  
Miftahuddin Azhari ◽  
Sandra Anggita Fadriana
Keyword(s):  
Response Surface ◽  
Extraction Process ◽  
Operating Conditions ◽  
Solvent Ratio ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Surface Method ◽  
Assisted Extraction ◽  
Order Polynomial ◽  
Independent Variable

Cockspur coral (Erythrina crista-galli L) petals are flowers that contain anthocyanins and active compounds of flavonoids and tannins. This study aims to determine the optimum conditions for the anthocyanin extraction process of cockspur coral petals using Microwave-Assisted Extraction (MAE), analyzed through the Response Surface Method (RSM). This process uses ethanol-hydrochloric acid solvents and a Box-Behnken experimental design involving three factors, namely the solvent ratios (w/v) (1:5, 1:15, and 1:25), microwave powers (300, 450, and 600 watts), and extraction times (3, 9, and 15 minutes). As a result, the second-order polynomial model was enhanced and sufficient to explain the variation of the data that denoted the significant correlation with the independent variables and the response. Derringer's desired function methodology was used for optimizing studies and generated ideal conditions for each or combined an independent variable.  The optimum anthocyanin extract of 5.82 mg/L was obtained at a power condition of 325,5 Watts, an extraction time of 3.05 minutes, and a solvent ratio of 20.5. Meanwhile, the operating conditions at a power of 310.8 Watts, a time of 14.94 minutes, and a solvent ratio of 24.96 resulted in the optimum color intensity (IC) of 1040.26. In the meantime, the optimum antioxidant activity was obtained at a power of 585.97 Watts, a time of 4.93 minutes, and a solvent ratio of 5.43 with IC50 of 0.115.

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