scholarly journals EKSTRAKSI FLAVONOID DARI DAUN KERSEN (Muntinga calabura L) MENGGUNAKAN PELARUT ETANOL DENGAN METODE MAE (Microwave Assisted Extraction) DAN UAE (Ultrasonic Assisted Extraction)

2021 ◽  
Vol 6 (2) ◽  
pp. 105
Author(s):  
Siti Indana Isdiyanti ◽  
Laeli Kurniasari ◽  
Farikha Maharani
Keyword(s):  
Solvent Ratio ◽  
Maximum Point ◽  
Microwave Assisted Extraction ◽  
Flavonoid Content ◽  
Ultrasound Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction ◽  
Ultrasound Assisted

Kersen (Muntinga calabura L) adalah pohon yang memiliki buah kecil dan manis. Tumbuhan ini dimanfaatkan antara lain sebagai  obat penurun panas, mengobati pembengkakan kelenjar prostat dan mengobati penyakit asam urat, selain itu juga dapat dimanfaatkan sebagai antiseptic, antioksidan, antimikroba, dan anti inflamasi. Berbagai macam metode ekstraksi telah dikembangkan dari yang konvensional ke metode modern, dimana salah satunya yaitu Microwave Assisted Extraction (MAE) dan Ultrasound Assisted Extraction (UAE). Penelitian ini bertujuan untuk menganalisa pengaruh metode ekstraksi yang dilakukan terhadap rendemen, menganalisa pengaruh rasio solid berbanding liquid serta waktu terhadap rendemen, dan menguji kadar flavonoid yang dihasilkan dari kedua metode tersebut. Percobaan dilakukan dengan variabel rasio-pelarut 1:5 – 1:25, sedangkan variabel waktu 5 menit – 25 menit. Hasil percobaan menunjukan bahwa ke dua variabel berpengaruh terhadap konsentrasi flavonoid hasil ekstraksi. Secara umum meningkat seiring kenaikan variabel sampai maksimum di titik tertentu, kemudian turun. Konsentrasi maksimum diperoleh pada variabel umpan-pelarut MAE sebesar 1:25 dengan kadar flavonoid 132,41 mg/ml dan rendemen sebesar 39%. Adapun waktu terbaik adalah 5 menit dengan kadar flavonoid 91,669 mg/ml dan rendemen sebesar 22,7%. Sedangkan pada proses UAE (Ultrasonic Assisted Extraction) dengan rasio umpan-pelarut maksimal sebesar 1:10 dengan kadar flavonoid 47,5899 mg/ml dan rendemen 26%. Sedangkan waktu terbaik  10 menit dengan kadar flavonoid 56,7769 mg/ml dan rendemen sebesar 19,65%. AbstracKersen  (Muntinga calabura L) is a tree with small and sweet fruit. The fruit is used, among others, as a febrifuge, treating swelling of the prostate gland and treating gout. Besides the fruit, its leaves also has potential as an antiseptic, antioxidant, antimicrobial, and anti-inflammatory. Various extraction methods have been developed from conventional to modern methods, one of which is Microwave Assisted Extraction (MAE) and Ultrasound Assisted Extraction (UAE). The research will extract the kersen leaves and aim to analyze the effect of the extraction method on the yield, the effect of the solid to liquid ratio and time on the yield, and the flavonoid content produced by both methods. The experiment was carried out with a solvent-ratio variable of 1:5 – 1:25, while the time variable was 5 – 25 minutes. The results of the experiment showed that the two variables had an effect on the concentration of flavonoids extracted. In general, it increases as the variables increase to a maximum point, then decreases. The maximum concentration obtained in the feed-solvent variable MAE was 1:25 with a flavonoid content of 132.41 mg/ml, with a yield of 39% and the best time was 5 minutes with a flavonoid content of 91.669 mg/ml, with a yield of 22.7%. While in the UAE Ultrasonic Assisted Extraction process with a maximum feed-solvent ratio of 1:10 with a flavonoid content 47.5899 mg/ml and yield  26%, while the best time of process was 10 minutes with  flavonoid content 56.7769 mg/ml and yield 19, 65%.Kata kunci: Flavonoid, Ekstrak Kersen, MAE, UA

scholarly journals Extraction Optimization of 5,7-Dihydroxy-6,8,4′-trimethoxyflavonol, a Bioactive Flavonoid from Rubus rosifolius (Rosaceae) Leaves

2019 ◽  
Vol 14 (1) ◽  
pp. 1934578X1901400
Author(s):  
Janieire Lorraine da Rocha Pittarello ◽  
Marcel Petreanu ◽  
Valdir Cechinel Filho ◽  
Clóvis Antonio Rodrigues ◽  
Luiz Carlos Klein-Júnior ◽  
...  
Keyword(s):  
Extraction Method ◽  
High Performance ◽  
Solvent Ratio ◽  
Flavonoid Content ◽  
Box Behnken Design ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction ◽  
Addition Level

Recently, it has been demonstrated that a rare flavonoid, 5,7-dihydroxy-6,8,4′-trimethoxyflavonol (1), isolated from the leaves of Rubus rosifolius showed significant antiproliferative activity against different cancer cell lines and had a diuretic effect. This study aimed to determine the best extraction method evaluating dynamic maceration extraction (DME), ultrasonic assisted extraction (UAE), and microwave assisted extraction (MAE). In addition, level optimization for different factors was focused using Design of Experiments. The flavonoid content was estimated by high performance liquid chromatography (HPLC). The results indicated that UAE provided the highest content with the shortest extraction time. For optimization, response surface methodology (RSM) was used, applying a Box-Behnken Design (BBD) with three factors at three levels. It was possible to observe that the flavonoid content significantly increased with the increase of temperature (max. 70°C). The time had little influence on the extraction and, for this reason, was set at 25 min. With regard to the drug/solvent ratio, it was observed that this had an impact on yield with a ratio of 3.5%. A model was built and a determination coefficient of 0.98 was obtained. After validation, an optimized method was developed, able to extract 1 with a lower time, lower solvent consumption, and affording higher contents.

scholarly journals Microwave-assisted extraction of Taxol and 10-Deacetyl baccatin III from the leaves and branches of red pine (Taxus wallichiana Zucc.)

2016 ◽  
Vol 19 (3) ◽  
pp. 76-87
Author(s):  
Phung Thi Kim Le ◽  
Quan Thi Hong Vu ◽  
Tuyet Thi Ngoc Nguyen ◽  
Khoa Anh Tran ◽  
Kien Anh Le
Keyword(s):  
Chloroform Extract ◽  
Red Pine ◽  
Solvent Ratio ◽  
Microwave Assisted Extraction ◽  
Baccatin Iii ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction ◽  
Short Time

Taxol has been by far the most well-known worldwide as an effective anticancer natural drug. With the great treatment abilities at low concentration, Taxol is a considerable interest of many scientists in various fields. The commercial products of Taxol can be isolated directly from Taxus species or can be synthesized from 10-deacetylbaccatin III (10-DAB III) or baccatin III (BC III), which are known as precursors of Taxol, by using semisynthetic methods. In this study, the extraction of Taxol and 10-DAB III from the leaves and branches of red pine cultivated in Lam Dong Province, Vietnam was carried out. Some traditional methods such as Soxhlet, maceration as well as the modern methods such as microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE) were used to extract and evaluate the extraction efficiency. The concentration of 10-DAB III experienced at over 90% as compared to maceration and the amount of Taxol accounted for approximately 80% of Soxhlet. MAE was thus more suitable for recovering both Taxol and 10-DAB III than the others because of its short time and less solvent consumption. Besides, four parameters including solvent nature (MeOH and EtOH), extraction time, material/solvent ratio (1:10, 1:15, 1:20 and 1:25) and microwave power (40W, 240W and 440W) were investigated the effects of these elements on the content of 10-DAB III and Taxol. The results illustrated that the optimal conditions providing 95.85% 10-DAB III were as follows: 240W, 1:15 ratio and 20 minutes for extraction. To get the highest amount of Taxol (79.83%), extraction was subjected at these conditions concluding 40W, 1:25 ratio and 20 minutes. Diaion, NP-silicagel were used to enrich taxol and 10-DAB III from methanol extract and chloroform extract, respectively. The highest concentration of Taxol and 10-DAB III constituted at 0.64% and 19.76% with NP-silicagel (PE: aceton, 7:3).

scholarly journals Microwave-Assisted Extraction and Ultrasound-Assisted Extraction of Bioactive Compounds from Grape Pomace

2020 ◽  
Vol 16 (1-2) ◽  
Author(s):  
Cassiano Brown da Rocha ◽  
Caciano Pelayo Zapata Noreña
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Grape Pomace ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Ultrasound Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Ultrasound Assisted

AbstractThe grape pomace is a by-product from the industrial processing of grape juice, which can be used as a source of bioactive compounds. The aim of this study was to separate the phenolic compounds from grape pomace using an acidic aqueous solution with 2 % citric acid as a solvent, using both ultrasound-assisted extraction, with powers of 250, 350 and 450 W and times of 5, 10 and 15 min, and microwave-assisted extraction using powers of 600, 800 and 1,000 W and times of 5, 7 and 10 min. The results showed that for both methods of extraction, the contents of total phenolic compounds and antioxidant activity by ABTS and DPPH increased with time, and microwave at 1,000 W for 10 min corresponded to the best extraction condition. However, the contents of phenolic compounds and antioxidant activity were lower than exhaustive extraction using acidified methanol solution.

scholarly journals Ziziphus lotus (L.) Lam. plant treatment by ultrasounds and microwaves to improve antioxidants yield and quality: An overview

2021 ◽  
Vol 5 (12) ◽  
pp. 53-68
Author(s):  
Farida Berkani ◽  
Maria Luísa Serralheiro ◽  
Farid Dahmoune ◽  
Malik Mahdjoub ◽  
Nabil Kadri ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Extraction Yield ◽  
Point Of View ◽  
Microwave Assisted Extraction ◽  
Ultrasound Assisted Extraction ◽  
Microwave Assisted ◽  
Yield And Quality ◽  
Assisted Extraction ◽  
Industrialization Process ◽  
Ultrasound Assisted

The purpose of this review is to compile the literature published about different aspects of microwave-assisted extraction (MAE) use and ultrasound-assisted extraction (UAE) applied on jujube worldwide and to compare the results on the antioxidant activity obtained for each extraction method. As a result of the increased consumers demand for natural products, as well as for those of agro-food, nutraceutical, cosmetic industries, and green extraction techniques are nowadays trending to be potential alternatives that can improve antioxidant yield and its quality from an economical and environmental point of view by reducing time, energy, and solvent consumption. Ultrasounds and microwaves are widely used methods in the extraction of active principles due to their cavitation and dipolar rotation effect, respectively. These two techniques provide efficiency of extraction while minimizing the time and preserving the quality of the food matrix, overcoming the disadvantages of conventional techniques characterized by their consumption of large quantities of solvents and providing a sparse quantity of extraction. Jujube, a shrub with a high antioxidant potential, which can be affected by various extraction conditions can be the target of UAE and MAE to increase the antioxidant extraction yield. Exploiting the beneficial properties such as the antioxidant activity can lead to an industrialization process, replacing therefor synthetic antioxidants with natural compounds. These can also help in the development of new nutraceuticals and can be used, for instance, in agro-food industries as preservatives. Keywords : Microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE), antioxidants, Ziziphus lotus (L.) Lam plant.

scholarly journals Aqueous extraction of anions from coal and fly ash followed by ion-chromatographic determination

2016 ◽  
Vol 81 (12) ◽  
pp. 1441-1453
Author(s):  
Aleksandra Tasic ◽  
Ivana Sredovic-Ignjatovic ◽  
Ljubisa Ignjatovic ◽  
Danijel Djuranovic ◽  
Malisa Antic
Keyword(s):  
Fly Ash ◽  
Ph Value ◽  
Chromatographic Determination ◽  
Aqueous Extraction ◽  
Microwave Assisted Extraction ◽  
Ultrasound Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Coal Particles ◽  
Ultrasound Assisted

Three different techniques were applied for the aqueous extraction of anions from coal and fly ash: rotary mixer- and ultrasonic-assisted extraction with different duration time, and microwave-assisted extraction at different temperatures. Validation showed that the ion-chromatographic method was suitable for the analysis of anions in coal and fly ash extracts. The variations in the amounts of anions using different extraction times during rotary-assisted extraction were minimal for all investigated anions. The efficiency of ultrasound-assisted extraction of anions from coal depended on the sonication time and was highest at 30 min. The ultrasound-assisted extraction was less efficient for the extraction of anions from fly ash than rotary-assisted extraction. Increase of temperature in the microwave-assisted extraction had a positive effect on the amounts of all anions extracted from coal and sulphate from fly ash, while the amounts of fluoride and chloride in fly ash extracts decreased. The microwave-assisted extraction of coal at 150?C was compared with standard ASTM methods, and results were in good agreement only for chloride. Changes in the pH value and conductivity during ultrasound-assisted extraction were measured in order to explain changes on the surface of coal particles in contact with water and different processes that occur under environmental conditions.

scholarly journals Optimization Ultrasonic Assisted Extraction (UAE) of Bioactive Compound and Antibacterial Potential from Sea Urchin (Diadema Setosum)

2020 ◽  
pp. 556-569
Author(s):  
Muhammad Yusuf ◽  
Nur Fitriani Usdyana Atthamid ◽  
Sri Indriati ◽  
Rahmawati Saleh ◽  
Mahyati Latief ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Sea Urchin ◽  
Bioactive Compound ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction ◽  
Ultrasound Assisted ◽  
Diadema Setosum

Sea urchins can be used in medicine, has potential as a new type of antibiotic to developed in the pharmaceutical field, which is rich in bioactive compounds as a steroid, triterpenoids, saponins, and antioxidant properties. Conventional extraction generally takes a long time, is less environmentally friendly, and potentially triggers bioactive compound damage, so it needs alternative methods such as Ultrasound-Assisted Extraction (UAE). The extracting technology, including ultrasonic-assisted extraction (UAE) and solvent variation (ethyl acetate and methanol) of bioactive compounds from sea urchin (Diadema setosum) were optimized and compared. The purpose of this study was to study the application of UAE and solvent variation methods for sea urchin extraction from the Barrang Lompo Island in South Sulawesi. Comparing the maceration and ultrasonic assisted extraction methods. Optimization of extraction with UAE was carried out on the variable of extraction duration and type of solvent. Gas Chromatography-Mass Spectrometry results show that ultrasound-assisted extraction generally produces compounds of CHOLEST-5-EN-3-OL (3. BETA.), palmitic acid, 9-Octadecenoic acid (Z) -, methyl ester, stearic acid, oleic acid, flavonoids, phenols, pentadecanoic acid and batilol and streoid, which has a function as an antioxidant, anti-inflammatory, anti-tumor, anti-cancer agents and antibacterial. The results showed the best results using ultrasound-assisted extraction with a duration of 30 minutes and using solvent ethyl acetate. These results implied that extracts obtained by sonication showed the highest bioactive compounds and antioxidant activity, thus proving that this activity depends directly on the antibacterial properties.

Sign in / Sign up

Export Citation Format

Share Document