scholarly journals The PHARMACOGNOSTICAL, PHYSICOCHEMICAL AND HIGH-PERFORMANCE THIN LAYER CHROMATOGRAHY (HPTLC) EVALUATION OF ERANDAMULADI KALKA CHOORNA

2020 ◽  
Vol 9 (5) ◽  
Author(s):  
Swathi N. ◽  
Anup B. Thakar ◽  
Harisha C.R. ◽  
V.J. Shukla
Keyword(s):  
Pharmaceutical Analysis ◽  
High Performance ◽  
Water Soluble ◽  
Phytochemical Analysis ◽  
Low Back ◽  
Soluble Extract ◽  
Physico Chemical ◽  
Common Ailment ◽  
Back Ache ◽  
Water Soluble Extract

Introduction: Erandamuladi Niruha Basti is mentioned in Charaka Samhita which is indicated in Trika, Prishta Shoola (low back ache) and acts as Maruta Nigraha. Low backache is the most common ailment in today’s busy life. Erandamuladi Kalka used in Basti contains 9 drugs in which most of the drugs are having Ushna Veerya and are Shoolahara in nature. Method: Sookshma Choorna of Erandamuladi Kalka was evaluated for their pharmacognostic and pharmaceutical analysis. Results: Pharmacognostic study showed the presence of contents such as cluster crystal of Hapusha, starch grains of Musta, spiral vessels of Bala etc. Physico-chemical analysis showed that the loss on drying 6.28%, Ash value 13.26%, Water soluble extract 19.34%, Alcohol soluble extract 4.24%, pH 7.0 Discussion and Conclusion: The pharmacognostical and phytochemical analysis of Erandamuladi Kalka Choorna confirmed the purity and genuinity of drug, which can be espoused for laying down the standards of it. Keywords: Erandamuladi Kalka Choorna, Pharmacognostic, Phytochemical analysis, HPTLC

scholarly journals Pharmacognostical, phytochemical and HPTLC evaluation of Erandamuladi Kwatha Churna - A polyherbal formulation for pharmaceutical standardization

2020 ◽  
Vol 5 (05) ◽  
pp. 191-195
Author(s):  
Swathi N. ◽  
Anup B. Thakar ◽  
Harisha C.R. ◽  
V.J. Shukla
Keyword(s):  
Pharmaceutical Analysis ◽  
Study Data ◽  
Pollen Grain ◽  
Water Soluble ◽  
Low Back ◽  
Soluble Extract ◽  
Polyherbal Formulation ◽  
Physico Chemical ◽  
Back Ache ◽  
Water Soluble Extract

Introduction: Erandamuladi Kwatha is mentioned in Ayurveda classics as a therapeutic formulation of Basti mainly to treat Trika-prishta Shoola (low back ache). The most common disorder which affects the movement of leg particularly in the productive period of life is low backache. Erandamuladi Kwatha used in Basti contains 15 drugs in which most of the drugs are having Ushna Veerya and are Vatakaphahara in nature. Materials and Methods: Yavakuta Choorna of Erandamuladi Kwatha was evaluated for their pharmacognostic and pharmaceutical analysis. Results and Discussion: Pharmacognostic study showed the presence of contents such as starch grains of Kantakari, rosette crystal of Rasna, pollen grain of Atibala etc. Physico-chemical analysis showed that the loss on drying 7.74%, Ash value 6.78%, Water soluble extract 20.12%, Alcohol soluble extract 4.3%, pH 7.2. Conclusion: From the study, data developed can be espoused for laying down the standards of Erandamuladi Kwatha.

scholarly journals Pharmacognostical and pharmaceutical analysis of Punarnava guggulu an ayurvedic polyherbal formulation

2020 ◽  
Vol 7 (6) ◽  
pp. 989
Author(s):  
Chaitali Kakadiya ◽  
Mandip Goyal ◽  
C. R. Harisha ◽  
V. J. Shukla
Keyword(s):  
Pharmaceutical Analysis ◽  
High Performance ◽  
Ph Value ◽  
Water Soluble ◽  
Soluble Extract ◽  
Polyherbal Formulation ◽  
Weight Variation ◽  
Physico Chemical ◽  
Microscopic Evaluation ◽  
Water Soluble Extract

Background: Punarnava Guggulu is a polyherbal formulation mentioned in Bhaishajya Ratnavali containing various Ayurvedic medicinal drugs and specially indicated for the treatment of Amavata and Vatarakta. For assurance of quality of herbal compounds pharmacognostical and pharmaceutical analysis should be done.   Methods: Punarnava Guggulu was subjected to microscopic evaluation for Pharmacognostical study, physiochemical analysis like hardness, weight variation, loss on drying, ash value, acid insoluble extract, pH value, water soluble extract, alcohol soluble extract, high performance thin layer chromatography (HPTLC).Results: Pharmacognostical study showed the presence of certain identifying characters of all of the ingredients of Punarnava Guggulu that is Punarnava, Erandamula, Shunthi, Guggulu, Eranda Tail, Trivruta, Danti, Guduchi, Haritaki, Bibhitaki, Amalaki, Maricha, Pippali, Chitraka, Bhallataka and Vidanga. In pharmaceutical study, preliminary physiochemical analysis showed that hardness of the Vati was 4.05 Kg/cm2, ash value 12.84% w/w, acid insoluble ash value 1.56% w/w, loss on drying 1.6% w/w, water soluble extract 35.93% w/w, alcohol soluble extract 22.14% w/w and HPTLC showed 13 spots in 254nm and 8 spots in 366nm.Conclusions: Present work was carried out to standardize the polyherbal formulation Punarnava Guggulu in terms of its identity, quality and purity. Pharmacognostical and physico-chemical observations revealed the specific characters of all active constituents in the preparation were present in it.

scholarly journals Pharmacognostical and pharmaceutical analysis of Triphala Kajjali tablet-an ayurvedic herbomineral formulation for metabolic syndrome

2021 ◽  
Vol 8 (2) ◽  
pp. 195
Author(s):  
Jaynika S. Garasia ◽  
Mandip Goyal ◽  
Harisha C. R. ◽  
Vinay J. Shukla
Keyword(s):  
Pharmaceutical Analysis ◽  
High Performance ◽  
Ph Value ◽  
Fine Powder ◽  
Water Soluble ◽  
Soluble Extract ◽  
Terminalia Bellerica ◽  
Weight Variation ◽  
Microscopic Evaluation ◽  
Water Soluble Extract

Background: Triphala is one of the most easily available and commonly used medicine which contains fine powder of three fruits viz. Terminalia chebula Retz. (Haritaki), Terminalia bellerica Roxb. (Bibhitaki) and Embilica officinalis Gaertn. (Amalaki) and is indicated as one of the drugs for management of disorders of Kapha and Meda. Due to bitter and astringent taste of Triphala in the powder form is a major complaint of the patients. So, to discover that form of Triphala, which is easy to take, effective in low dose, has long shelf life and simple to dispense is the need. For assurance of quality of herbal compounds pharmacognostical and pharmaceutical analysis should be done. Methods: Triphala Kajjali was subjected to microscopic evaluation for pharmacognostical, physiochemical analysis like hardness, weight variation, loss on drying, ash value, acid insoluble extract, pH value, water soluble extract, alcohol soluble extract and high-performance thin layer chromatography (HPTLC).Results: Pharmacognostical study showed the presence of certain identifying characters of all of the ingredients of Triphala Kajjali that is Haritaki, Bibhitaki and Amalaki. In pharmaceutical study, preliminary physiochemical analysis showed that hardness of the tablet was 2.05 kg/cm2, ash value 4.03%w/w, loss on drying 5.5%w/w, water soluble extract 5.89%w/w, alcohol soluble extract 25.96%w/w and HPTLC showed 7 spots in 254 nm and 7 spots in 366 nm.Conclusions: Pharmacognostical and physico-chemical observations revealed the specific characters of all active constituents of Triphala Kajjali and confirmed the purity and genuinity of the drug.

scholarly journals Quality Control Evaluation of an Ayurvedic Herbomineral Compound – Mukkadi Bidalaka

The Healer ◽  
2021 ◽  
Vol 2 (1) ◽  
pp. 52-57
Author(s):  
Sangita ◽  
Neethu Kesavan ◽  
D.B. Vaghela
Keyword(s):  
High Performance ◽  
Ph Value ◽  
Morphological Characters ◽  
Water Soluble ◽  
Physicochemical Study ◽  
Soluble Extract ◽  
Parenchyma Cells ◽  
Prismatic Crystal ◽  
Control Evaluation ◽  
Water Soluble Extract

Background: Mukkadi Bidalaka Yoga is a herbomineral compound which is claimed to be effective in Madhumehajanya Timira (Diabetic Retinopathy).  In present study, it has been used for Bidalaka Karma.Objective: Present study was designed to watch out on herbal and mineral drugs used in the preparation of Mukkadi Bidalaka Yoga to assess the quality, purity, safety and efficacy of the drug by pharmacognostical (microscopic) study, physicochemical study and chromatographic evaluation. Methods: Pharmacognostical study i.e. morphological characters, organoleptic characters and powder microscopy was done for identification and authentication of all the raw drugs. Physicochemical study and HPTLC of final product were done. Results: Pharmacognostical characters of Mukkadi Bidalaka observed under the microscope were epicarp cells and lignified stone cell of Haritaki, Trichome and lignified scleroids of Bibhitaki,  Scleroids, silica deposition of Amalaki, Parenchyma cells and starch grain of Haridra, Prismatic crystal, pitted vessels and fibers of Daruharidra, Cork cells  of Nimba, Brown content of Sariva, Trichome, oil globule of Usheera, Stone cells, scleroids, prismatic crystal and fibers of Lodhra, Cork cells , fibers  and stone cells of Vata, Parenchyma cells and oil globule of Durva, Border pitted vessels, lignified stone cells oil globule and lignified fiber passing through medullary rays of Chandana, Particles of Girika. Border pitted vessels, lignified fiber, lignified fiber passing through medullary rays of Raktachandana. Pharmaceutical evaluation of Mukkadi Bidalaka Yoga showed results loss on drying at 110C 5.85 % w/w, pH value 6.0, water soluble extract 21.60% w/w, methanol soluble extract 14.28% w/w, Ash value 3% w/w acid insoluble ash 0.39% w/w. High Performance Thin Layer Chromatography result shows 9 spots at 254 nm and 7 spots at 366 nm. Conclusion: Identification and authentication of herbal drug used in the preparation of Mukkadi Bidalaka Yoga was done. Pharmacognostical, physicochemical and HPTLC evaluation of prepared drug has been carried out.

PHARMACOGNOSTICAL, PHYSICOCHEMICAL AND HPTLC EVALUATION OF AMRUTADHYA GUGGULU A POLYHERBAL FORMULATION FOR PHARMACEUTICAL STANDARDIZATION

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Divya Zala ◽  
Dilip Prajapati ◽  
Anup Thakar ◽  
Harisha CR ◽  
V.J.Shukla
Keyword(s):  
Pharmaceutical Analysis ◽  
Quality Standard ◽  
Water Soluble ◽  
Soluble Extract ◽  
Traditional Medicines ◽  
Polyherbal Formulation ◽  
Characteristic Features ◽  
Increasing Demand ◽  
Water Soluble Extract

Background: Standardization of herbal formulation is needed in order to assess of quality of drug. Maintaining the quality standard of a polyherbal formulation is a difficult task. In the era of increasing demand for traditional medicines, maintaining quality standards is the need of the hour. Aim: The present work was carried out to standardize the finished product Amrutadhya Guggulu to confirm its identity, quality and purity. Material and Method: Amrutadhya Guggulu is mentioned in the treatment of Sthaulya (Obesity), and it was prepared according to the method as described in Chakradatta. Amrutadhya Guggulu powder was evaluated for its pharmacognostic and pharmaceutical analysis. It contains drug like Guduchi, Ela, Kutaja Phala, Kutaj twaka etc. Result: Epicarp and stone cell of Vidanga, silica deposition of Amalaki, oil globule of Ela etc were the characteristic features observed in the microscopy of Amrutadhya Guggulu powder .Results found in pharmaceutical parameters of Amrutadhya Guggulu powder like Loss on drying8.07% w/w, Ash value 3.07% w/w, Water soluble extract 22.5 % w/w, alcohol soluble extract 13.44%w/w and pH 4 Conclusion: The Obtained values of all parameters for the finish product can be adopted to set down new standards. Keywords:  Amrutadhya Guggulu, HPTLC, Pharmacognostical, Pharmaceutical analysis

scholarly journals PHARMACOGNOSTICAL, ANALYTICAL STUDY AND HPTLC EVALUATION OF SHAMPAKADI KWATHA, A POLYHERBAL FORMULATION FOR PHARMACEUTICAL STANDARDIZATION

2019 ◽  
Vol 8 (6) ◽  
Author(s):  
Rajdip Rao ◽  
Anup Thakar ◽  
Harisha CR ◽  
V.J. Shukla
Keyword(s):  
Back Pain ◽  
Pharmaceutical Analysis ◽  
Analytical Study ◽  
Study Data ◽  
Low Back ◽  
Soluble Extract ◽  
Cassia Fistula ◽  
Lumbar Spondylosis ◽  
Polyherbal Formulation ◽  
Common Ailment

Background: Shampakadi kwatha is mentioned in Ayurvedic classics as a therapeutic formulation of Basti to treat Musculo-skeletal diseases like Katishool (Lumbar spondylosis). Back pain is the most common ailment in today’s busy life. Majority of us have experienced one or more episodes of back pain in our lives and many of us live with chronic symptoms. Incidences of low back pain affects 60-85% in adults and lumbar Spondylosis is responsible for about 10% of all back pain. Shampakadi kwatha contains Aragvadha (Cassia fistula Linn.), Eranda (Ricinus communis.Linn), Punarnava (Boerhavi diffusa Linn) etc. which have Shoolaghna and Vatahara property. Method: Shampakadi kwatha powder was evaluated for their pharmacognostic and pharmaceutical analysis. Results: Microscopic characters were found of all the contents of Shampakadi kwatha. Results obtained in pharmaceutical parameters of Shampakadi kwatha powder like loss on drying 12.18%, Ash value 9.06%, Alcohol soluble extract 62.15% w/v etc. HPTLC profile of Shampakadi kwatha powder showed similarities in number of spots. Conclusion: From the study, data developed can be espoused for laying down the standards for Shampakadi kwatha. Keywords:  HPTLC, Pharmacognostical, Pharmaceutical analysis, Shampakadi Kwatha

Pharmacognostical Standardization and Pharmacological Potential of Elaeocarpus ganitrus Leaves as an Antiulcer Agent

2018 ◽  
Vol 11 (4) ◽  
pp. 4162-4169
Author(s):  
Mayank Kulshreshtha ◽  
Manjul Pratap Singh
Keyword(s):  
Skin Diseases ◽  
Scientific Data ◽  
Water Soluble ◽  
Histopathological Analysis ◽  
Phytochemical Analysis ◽  
Dependent Manner ◽  
Soluble Extract ◽  
Biochemical Studies ◽  
Pharmacological Potential ◽  
Dose Dependent

Elaeocarpus ganitrus Roxb, (E. ganitrus) known as Rudraksha belongs to family- Eleocarpaceae. It has a reflecting position in Hinduism and Ayurveda whereas traditionally it has mentioned to cure various health problems like fever, skin diseases, mental problems, wound healing etc. The present study was designed to study the microscopic and macroscopic analysis, physiochemical parameters, quantitative microscopy, phytochemical screening of E. ganitrus leaves as per WHO guidelines and evaluate the antiulcer potential of aqueous extract of E. ganitrus (AEEG) and ethanolic extract of E. ganitrus (EEEG) at the doses of 200 mg/kg and 400 mg/kg using pylorus ligation induced ulcers model, biochemical parameters. Hepatic, cardiac, hematological parameters have also done to find out the effect of different extracts on other major organs. Microscopic analysis proved the presence of covering trichomes, upper epidermis, lower epidermis, stomata, phloem, xylem etc. Ash value, water soluble ash, acid soluble ash, water soluble extract, alcohol soluble extract, loss on drying, swelling index, foaming index found to be 4.3 ± 0.52, 0.2 ± 0.33, 2.0 ± 0.2, 13.7 ± 0.25, 12.5 ± 0.55, 9.8 ± 0.23, 3.6 ± 0.04, more than 100. Different quantitative parameters were found out. Phytochemical analysis of different extracts showed the presence of various primary and secondary metabolite like alkaloids, glycosides, tannin, phenolic compounds etc. Pharmacological potential showed that extracts treated, and sucralfate treated groups showed significantly decreases in ulcer index in all above-mentioned models, biochemical studies clearly showed significant decreases in volume, pH, free acidity, total acidity of gastric content and increases in gastric mucus parameters like protein, total hexoses, hexosamine, fucose, sialic acid and DNA level. The level of antioxidant enzymes like LPO (Lipid peroxidation), SOD (Superoxide dimutase) were decreased and CAT (Catalase) level was increased. Level of PC (Plasma corticosterone) was decreased. Hematological, hepatic, cardiac parameters found to be normal during extracts treatment. Histopathological analysis clearly supports the biochemical studies at various doses and it was found to be effective in dose dependent manner. The obtained scientific data may be helpful to prepare the monograph of the plant and E. ganitrus has antiulcer potential in a dose dependent. Detailed study needed for better exposure of plant.

Prebiotic frozen dessert processed with water‐soluble extract of rice byproduct: Vegan and nonvegan consumers perception using preferred attribute elicitation methodology and acceptance

2021 ◽  
Vol 86 (2) ◽  
pp. 523-530
Author(s):  
Jiuliane Martins da Silva ◽  
Carlos Eduardo Barão ◽  
Erick Almeida Esmerino ◽  
Adriano Gomes Cruz ◽  
Tatiana Colombo Pimentel
Keyword(s):  
Water Soluble ◽  
Soluble Extract ◽  
Frozen Dessert ◽  
Water Soluble Extract

Activation of Selective Transcription Factors and Cytokines by Water-Soluble Extract from Lentinus lepideus

2003 ◽  
Vol 228 (6) ◽  
pp. 749-758 ◽  
Author(s):  
Mirim Jin ◽  
Hyung Jin Jung ◽  
Jeong June Choi ◽  
Hyang Jeon ◽  
Jin Hwan Oh ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Post Treatment ◽  
Water Soluble ◽  
Dependent Manner ◽  
Soluble Extract ◽  
Transient Transfection Assay ◽  
Gm Csf ◽  
Tnf Α ◽  
Water Soluble Extract

We isolated a water-soluble extract, PG101, from cultured mycelia of Lentinus lepideus. Treatment of human peripheral blood mononuclear cells (PBMCs) with PG101 increased levels of TNF-α, IL-1β, IL-10, and IL-12 by 100- to 1000-fold, whereas GM-CSF and IL-18 were activated by an order of magnitude. On the contrary, IFN-γ and IL-4 were not affected. The response to PG101 occurred in a dose- and time-dependent manner. From the human PBMCs treated with PG101, TNF-α was a first cytokine to be activated, detectable at 2 hr post-treatment followed by IL-1β at 6 hr post-treatment. IL-12 and IL-10 were the next to follow. GM-CSF and IL-18 both showed significant increases 24 hr after treatment. When PBMCs were sorted into various cell types, monocyte/macrophages, but not T and B cells, were the major target cell type responsive to PG101. Consistent with this result, the profile of cytokine expression upon PG101 treatment was comparable between PBMCs and a human promonocytic cell line (U937), whereas cell lines of T cell and myeloid origins did not respond to PG101. Data from a transient transfection assay involving specific reporter plasmids indicated that cellular transcription factor such as NF-κB, but not AP-1, was highly activated by PG101. Results from a gel retardation assay and the experiment involving a specific NF-κB inhibitor confirmed the involvement of NF-κB. Despite its significant biological effect on various cytokines, PG101 remained nontoxic in both rats and PBMCs even at a biological concentration approximately 20 times greater. PG101 demonstrates great potential as a therapeutic immune modulator.

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