scholarly journals The Protective Effect of Croton zambesicus against Carbon Tetrachloride-induced Renal Toxicity in Rats

2019 ◽  
Vol 13 (1) ◽  
pp. 5-8
Author(s):  
Rashidat Oluwafunke Ayanniyi ◽  
◽  
Hidayah Ayodeji Olumoh-Abdul ◽  
Fatimoh Idowu Ojuade ◽  
Rasheed Abdullahi ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Kidney Disease ◽  
Protective Effect ◽  
Leaf Extract ◽  
Renal Toxicity ◽  
Kidney Tissue ◽  
Albino Rats ◽  
Serum Electrolytes ◽  
Butanol Fraction ◽  
Aqueous Leaf Extract

Background: The leaf extract of Croton zambesicus (CZ) is used in traditional medicine for the management of various conditions including kidney disease. The purpose of this study was to determine the protective effects of leaf extract of CZ on rat kidney toxicity induced by carbon tetrachloride. Method: Male albino rats were divided into 6 groups of 5 rats and treated for 5 days with aqueous extract (200-400 mg/kg) and n-butanol fraction (20-40 mg/kg) of CZ. Carbon tetrachloride (2 ml/kg of 40% in olive oil) was administered subcutaneously for 3 days and the rats were treated with the extract and fraction for 3 days. Animals were euthanized on day 8 with intraperitoneal injection of ketamine (60 mg/kg) and xylazine (7.5 mg/kg). Blood sample was collected for the analysis of serum electrolytes, urea and creatinine. Kidney tissue was harvested to determine the antioxidant enzyme activity. Results: Carbon tetrachloride produced increased serum sodium ion, chloride ion, urea and creatinine with decreased superoxide, catalase, glutathione, oxidized glutathione and an increase in malondialdehyde concentration. Treatment of rats with the aqueous leaf extract and n-butanol fraction attenuated the toxic effects of carbon tetrachloride on kidney with a significant decline in serum electrolytes, urea, creatinine and a significant increase in the concentration of antioxidant enzymes. Conclusion: Results from this study revealed that the aqueous leaf extract and n-butanol fraction of CZ had protective effect against carbon tetrachloride-induced renal toxicity in rats, thus justifying the consideration of this plant for the management of kidney disease.

scholarly journals HEPATOPROTECTIVE AND ANTI-HEPATOTOXIC ACTIVITIES OF AQUEOUS LEAF EXTRACT OF TACAZZEA BARTERI AGAINST CARBON TETRACHLORIDE INDUCED HEPATOTOXICITY IN ALBINO RATS

2013 ◽  
Vol 2 (9) ◽  
pp. 60-65 ◽  
Author(s):  
Obioha Mary Quinette Uru ◽  
Ilodigwe Emmanuel Emeka ◽  
Ajaghaku Daniel Lotanna ◽  
Umeokoli Blessing Ogechukwu
Keyword(s):  
Carbon Tetrachloride ◽  
Leaf Extract ◽  
Albino Rats ◽  
Aqueous Leaf Extract

scholarly journals Hepatoprotective Effect of Senna mimosoides Aqueous Leaf Extract Against Carbon Tetrachloride Induced Hepatotoxicity in Albino Rats

2020 ◽  
Vol 13 (3) ◽  
pp. 100-106
Author(s):  
Nworah Florence N ◽  
Nkwocha Chinelo Ch ◽  
Nwachukwu Justus Nma ◽  
Nwodo Okwesili Fred Chile
Keyword(s):  
Carbon Tetrachloride ◽  
Leaf Extract ◽  
Hepatoprotective Effect ◽  
Albino Rats ◽  
Aqueous Leaf Extract

scholarly journals Protective Effect of N-Acetyl Cysteine on Moringa Oleifera Aqueous Leaf Extract-Induced Hepatic Toxicity in Wistar Albino Rats

2020 ◽  
Vol 8 (3) ◽  
pp. 34-39
Author(s):  
Namaganda Agnes ◽  
Kasolo N Josephine ◽  
Bbosa S. Godfrey ◽  
Lukande Robert ◽  
Kimuli Ivan ◽  
...  
Keyword(s):  
Protective Effect ◽  
Total Protein ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Albino Rats ◽  
P Value ◽  
Protein Levels ◽  
Aqueous Leaf Extract ◽  
Acetyl Cysteine ◽  
Wistar Albino Rats

Background: Moringa oleifera (M. oleifera) is a commonly used medicinal and nutritive plant. The aqueous leaf extract from this plant contains a high concentration of alkaloids and they are toxic to body organs especially the liver leading to hepatotoxicity after long term exposure. However, if N-Acetyl cysteine (NAC) is administered together with M. oleifera extract, it may have a hepatotoxic protective effect. Objective: To establish the protective effect of N-Acetyl cysteine against M. oleifera aqueous leaf extract-induced hepatotoxicity in the Wistar albino rats. Methods: An experimental laboratory-based study conducted at department of Physiology Makerere University, College of Health Sciences. Three treatment groups of six Wistar albino rats each, were dosed intragastrically once a day for 28 days. Group I; negative control, received 8.05g/kg bwt of M. oleifera extract plus 1ml of normal saline (NS), Group II; test group, received 8.05g/kg bwt of M. oleifera extract plus 50mg/kg of NAC. Group III; positive control, received 750mg/kg bwt of Paracetamol plus 50mg/kg of NAC. On the 14th and 29th day, three animals selected randomly from each group were sacrificed; blood samples were collected, the liver was harvested for histopathological analysis.  Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), bilirubin and total protein levels were determined. Results: There was an increase in serum ALT, AST and ALP levels in the M. oleifera extract plus NS group which was a sign of hepatotoxicity. The M. oleifera extract plus NAC group showed normal serum ALT, AST and ALP levels with no significant changes in the bilirubin (P-value = 0.9089) and total protein levels (P-value = 0.8858). Conclusion: The results have provided evidence that NAC administration with M. oleifera extract effectively prevents the occurrence of M. oleifera leaves xtract- induced hepatotoxicity.  

scholarly journals In vitro Antioxidant and In vivo Hepatocurative and Nephrocurative Activities of Aqueous Leaf Extract of Newbouldia laevis in Albino Rats

2021 ◽  
pp. 114-125
Author(s):  
Mohammed A. Sulaiman ◽  
Mahmoud S. Jada ◽  
Augustine Elizabeth ◽  
Abubakar Umar Modibbo
Keyword(s):  
Oral Administration ◽  
Leaf Extract ◽  
Kidney Diseases ◽  
Albino Rats ◽  
Protein Levels ◽  
Ameliorative Effect ◽  
Liver And Kidney ◽  
Aqueous Leaf Extract

The in vitro antioxidant activity and in vivo hepatocurative and nephrocurative potential of Newbouldia laevis aqueous leaf extract (NLALE) was evaluated. The study used 30 male, albino rats (Rattus norvegicus) weighing 180 ± 20 g, of which 25 were intoxicated by oral administration of a single dose of diclofenac (100 mg/kg b. wt.). Animals were treated by oral administration of silymarin (200 mg/kg b. wt.), furosemide (1.5 mg/kg b. wt.) and NLALE (200 mg/kg and 400 mg/kg b. wt.) for seven consecutive days before animals were sacrificed on the 8th day and serum/plasma was analyzed for biochemical markers of hepatotoxicity and nephrotoxicity. Phytochemical screening of NLALE revealed the presence of alkaloids, flavonoids, glycosides, phenols, saponins, steroids and tannins. The extract scavenged DPPH radical, reduced Fe3+ and inhibited TBARs in comparable manner to ascorbic acid in vitro. NLALE also attenuated diclofenac-induced liver and kidney intoxication as indicated by the significantly (p<0.05) reduced levels of serum biomarkers of hepatotoxicity: ALT, AST, bilirubin, but increased total protein levels and nephrotoxicity: urea, creatinine, Na+ and K+. The observed effects are dose dependent as the 400 mg/kg b. wt. appeared to be more potent than the 200 mg/kg b. wt. dose. It may be concluded from this study that Newbouldia laevis leaf has ameliorative effect against diclofenac-induced hepatotoxicity and nephrotoxicity probably through antioxidative mechanism and the curative claim and the folkloric use of the plant in the treatment of liver and kidney diseases have been scientifically validated

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