scholarly journals EFEK ANTIBAKTERI EKSTRAK ETANOL DAUN AFRIKA (VERNONIA AMYGDALINA) SEBAGAI BAHAN ALTERNATIF MEDIKAMEN SALURAN AKAR TERHADAP PORPHYROMONAS GINGIVALIS

2014 ◽  
Vol 18 (1) ◽  
pp. 48
Author(s):  
Cut Nurliza ◽  
Fitri Yunita Batubara ◽  
Tiurma Sitompul
Keyword(s):  
Porphyromonas Gingivalis ◽  
Vernonia Amygdalina ◽  
Drop Plate

Perawatan saluran akar memerlukan bahan medikamen saluran akar untuk mengeliminasi mikroorganisme yangtidak dapat dicapai dengan teknik preparasi chemo-mechanical. Salah satu bakteri yang sering ditemukan padainfeksi endodontik primer adalah Porphyromonas gingivalis. Daun Afrika (Vernonia amygdalina) merupakansalah satu bahan alami yang bersifat antibakteri yang dapat dijadikan sebagai bahan alternatif medikamensaluran akar. Tujuan penelitian eksperimental laboratoris ini adalah untuk mengetahui efek antibakteri ekstraketanol daun Afrika terhadap bakteri Porphyromonas gingivalis. Penelitian ini dimulai dengan melakukanekstraksi daun Afrika sebanyak 2 kg dengan pelarut etanol 70% hingga diperoleh ekstrak kental. Pengujian efekantibakteri menggunakan metode dilusi dengan mengencerkan ekstrak etanol daun Afrika dalam media MHBdimulai dari konsentrasi 100, 50, 25, 12,5, 6,25, dan 3,125% yang direplikasi 4 kali. Setiap konsentrasiditambahkan 1 ml suspensi bakteri, divorteks, dan diinkubasi 37°C selama 24 jam pada inkubator CO2 kemudiankekeruhan diamati dan dibandingkan dengan kontrol Mc Farland secara visual untuk menentukan nilai KHM.Kemudian dilanjutkan penghitungan jumlah koloni bakteri dengan metode Drop Plate Milles Misra untukmenentukan KBM yaitu setiap konsentrasi diambil 50 µl, diteteskan ke media MHA, direplikasi 4 petri,kemudian diinkubasi 37°C selama 24 jam. Sebagai kesimpulan, ekstrak etanol daun Afrika memiliki efekantibakteri terhadap Porphyromonas gingivalis secara in vitro dengan nilai KBM pada konsentrasi 50% dannilai KHM tidak representatif sehingga tidak dapat diketahui hasilnya.

Synergistic in-vitro anthelmintic potentials of Vernonia amygdalina Delile stem and Carica papaya Lin. seeds

2017 ◽  
Author(s):  
A Quartey ◽  
A Oppong ◽  
I Ayensu ◽  
J Apenteng ◽  
D Mintah ◽  
...  
Keyword(s):  
Carica Papaya ◽  
Vernonia Amygdalina

Lethal photosensitization of Porphyromonas gingivalis by their endogenous porphyrins under anaerobic conditions: An in vitro study

2013 ◽  
Vol 10 (4) ◽  
pp. 677-682 ◽  
Author(s):  
Christopher K. Hope ◽  
Jonathan A. Hindley ◽  
Zeeshan Khan ◽  
Elbert de Josselin de Jong ◽  
Susan M. Higham
Keyword(s):  
Porphyromonas Gingivalis ◽  
In Vitro Study ◽  
Vitro Study ◽  
Anaerobic Conditions

scholarly journals Abrogation of Neuraminidase Reduces Biofilm Formation, Capsule Biosynthesis, and Virulence of Porphyromonas gingivalis

2011 ◽  
Vol 80 (1) ◽  
pp. 3-13 ◽  
Author(s):  
Chen Li ◽  
Kurniyati ◽  
Bo Hu ◽  
Jiang Bian ◽  
Jianlan Sun ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Biofilm Formation ◽  
Adult Population ◽  
Transcriptional Analysis ◽  
Wild Type ◽  
Content Type ◽  
Multiple Organs ◽  
Biochemical Analyses

ABSTRACTThe oral bacteriumPorphyromonas gingivalisis a key etiological agent of human periodontitis, a prevalent chronic disease that affects up to 80% of the adult population worldwide.P. gingivalisexhibits neuraminidase activity. However, the enzyme responsible for this activity, its biochemical features, and its role in the physiology and virulence ofP. gingivalisremain elusive. In this report, we found thatP. gingivalisencodes a neuraminidase, PG0352 (SiaPg). Transcriptional analysis showed thatPG0352is monocistronic and is regulated by a sigma70-like promoter. Biochemical analyses demonstrated that SiaPgis an exo-α-neuraminidase that cleaves glycosidic-linked sialic acids. Cryoelectron microscopy and tomography analyses revealed that thePG0352deletion mutant (ΔPG352) failed to produce an intact capsule layer. Compared to the wild type,in vitrostudies showed that ΔPG352 formed less biofilm and was less resistant to killing by the host complement.In vivostudies showed that while the wild type caused a spreading type of infection that affected multiple organs and all infected mice were killed, ΔPG352 only caused localized infection and all animals survived. Taken together, these results demonstrate that SiaPgis an important virulence factor that contributes to the biofilm formation, capsule biosynthesis, and pathogenicity ofP. gingivalis, and it can potentially serve as a new target for developing therapeutic agents againstP. gingivalisinfection.

scholarly journals Establishment of in vitro fast-growing normal root culture of Vernonia amygdalina - a potent African medicinal plant

2009 ◽  
Vol 8 (21) ◽  
pp. 5952-5957 ◽  
Author(s):  
M Khalafalla M ◽  
M Daffalla H ◽  
A El Shemy H ◽  
Abdellatef E
Keyword(s):  
Medicinal Plant ◽  
Root Culture ◽  
Vernonia Amygdalina ◽  
Fast Growing

scholarly journals Porphyromonas gingivalis lipopolysaccharide stimulation in human periodontal ligament stem cells: role of epigenetic modifications to the inflammation

2017 ◽  
Vol 61 (3) ◽  
Author(s):  
Francesca Diomede ◽  
Soundara Rajan Thangavelu ◽  
Ilaria Merciaro ◽  
Monica D'Orazio ◽  
Placido Bramanti ◽  
...  
Keyword(s):  
Stem Cells ◽  
Porphyromonas Gingivalis ◽  
Inflammatory Disease ◽  
Periodontal Ligament ◽  
Model System ◽  
Epigenetic Mechanism ◽  
Periodontal Ligament Stem Cells ◽  
Porphyromonas Gingivalis Lipopolysaccharide

<p>Periodontitis is a chronic oral inflammatory disease produced by bacteria. Gingival retraction and bone and connective tissues resorption are the hallmarks of this disease. Chronic periodontitis may contribute to the risk of onset or progression of neuroinflammatory pathological conditions, such as Alzheimer’s disease. The main goal of the present study was to investigate if the role of epigenetic modulations is involved in periodontitis using human periodontal ligament stem cells (hPDLSCs) as an <em>in vitro</em> model system. hPDLSCs were treated with lipopolysaccharide of <em>Porphyromonas gingivalis</em> and the expression of proteins associated with DNA methylation and histone acetylation, such as DNMT1 and p300, respectively, and inflammatory transcription factor NF-kB, were examined. Immunofluorescence, Western blot and next generation sequencing results demonstrated that <em>P. gingivalis </em>lipopolysaccharide significantly reduced DNA methylase DNMT1, while it markedly upregulated the level of histone acetyltransferase p300 and NF-kB in hPDLSCs. Our results showed that <em>P. gingivalis </em>lipopolysaccharide markedly regulate the genes involved in epigenetic mechanism, which may result in inflammation induction. We propose that <em>P. gingivalis </em>lipopolysaccharide-treated hPDLSCs could be a potential in vitro model system to study epigenetics modulations associated with periodontitis, which might be helpful to identify novel biomarkers linked to this oral inflammatory disease.</p>

scholarly journals Glycation of Host Proteins Increases Pathogenic Potential of Porphyromonas gingivalis

2021 ◽  
Vol 22 (21) ◽  
pp. 12084
Author(s):  
Michał Śmiga ◽  
John W. Smalley ◽  
Paulina Ślęzak ◽  
Jason L. Brown ◽  
Klaudia Siemińska ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Biofilm Formation ◽  
Disease Process ◽  
Human Keratinocytes ◽  
Bacterial Biofilm ◽  
Pathogenic Potential ◽  
Glycated Proteins ◽  
Sds Page

The non-enzymatic addition of glucose (glycation) to circulatory and tissue proteins is a ubiquitous pathophysiological consequence of hyperglycemia in diabetes. Given the high incidence of periodontitis and diabetes and the emerging link between these conditions, it is of crucial importance to define the basic virulence mechanisms employed by periodontopathogens such as Porphyromonas gingivalis in mediating the disease process. The aim of this study was to determine whether glycated proteins are more easily utilized by P. gingivalis to stimulate growth and promote the pathogenic potential of this bacterium. We analyzed the properties of three commonly encountered proteins in the periodontal environment that are known to become glycated and that may serve as either protein substrates or easily accessible heme sources. In vitro glycated proteins were characterized using colorimetric assays, mass spectrometry, far- and near-UV circular dichroism and UV–visible spectroscopic analyses and SDS-PAGE. The interaction of glycated hemoglobin, serum albumin and type one collagen with P. gingivalis cells or HmuY protein was examined using spectroscopic methods, SDS-PAGE and co-culturing P. gingivalis with human keratinocytes. We found that glycation increases the ability of P. gingivalis to acquire heme from hemoglobin, mostly due to heme sequestration by the HmuY hemophore-like protein. We also found an increase in biofilm formation on glycated collagen-coated abiotic surfaces. We conclude that glycation might promote the virulence of P. gingivalis by making heme more available from hemoglobin and facilitating bacterial biofilm formation, thus increasing P. gingivalis pathogenic potential in vivo.

Sign in / Sign up

Export Citation Format

Share Document