Synergistic in-vitro anthelmintic potentials of Vernonia amygdalina Delile stem and Carica papaya Lin. seeds

2017 ◽  
Author(s):  
A Quartey ◽  
A Oppong ◽  
I Ayensu ◽  
J Apenteng ◽  
D Mintah ◽  
...  
Keyword(s):  
Carica Papaya ◽  
Vernonia Amygdalina

scholarly journals Evaluating the Synergistic Effects of Carica papaya Linn, Vernonia amygdalina Delile, Artemisinin and Iodine As Potential Agents in the Management of Breast Cancer

2018 ◽  
Vol 4 (Supplement 2) ◽  
pp. 122s-122s
Author(s):  
J. Nowak
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Leaf Extract ◽  
Carica Papaya ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Vernonia Amygdalina

Background: The rise in breast cancer prevalence rate across the globe and in Africa is a public health issue. Accessibility to the effective and affordable therapeutic options for the management of breast cancer in sub-Saharan Africa is challenging due to high costs, questionable effectiveness and toxicity of treatments available. Studies have documented the use of complimentary and alternative medicine in contemporary cancer treatment in form of standardized phytomedicines. However, the scientific evidence on their efficacies, synergy, safety and adequacy is still lacking. Vernonia amygdalina Delile (leaf extract). Furthermore, Carica papaya Linn (leaf extract), artemisinin and molecular iodine have been previously reported to have activity against breast cancer but their synergistic effect has not been validated. Aim: This study seeks to evaluate synergistic effects of Carica papaya Linn (leaf extract), Vernonia amygdalina Delile (leaf extract), artemisinin and molecular iodine against breast cancer cell lines in vitro and safety profile in selected animal models in vivo. Four specific objectives where stated: (i) to determine inhibitory concentration (IC50) of the plant and compound combinations in vitro against breast cancer cell lines; (ii) to determine effective dose (ED50) of most effective combination(s) against cancer cell lines using animal models (in vivo); (iii) to assess the acute, subacute, chronic toxicity profile of most efficacious combination against breast cancer cell lines; (iv) to identify and characterize bioactive compounds in the combination that shows the highest activity against breast cancer cell lines. Methods: Breast cancer cell lines: MCF 7, MDA-MB-231 will be used to determine IC50 of combinations in vitro. MTT assay will be applied for quantification of viable cells in culture. Both positive and negative controls will be adopted in the study. Mice and rats animal models will be used for toxicity study and determination of ED50 of most active combinations. Bioactive compounds in combination(s) will be identified using chromatographic and spectroscopic methods. Standard procedures according to WHO. Graph Prism computer software will be used to obtain LD50. The results will be statistically analyzed using Student t-test and one-way analysis of variance (ANOVA) using SPSS 16 (Statistical Package for Social Sciences) software. Results: Study in progress. Conclusion: This study will provide a significant contribution into aspects of herbal medicine in breast cancer research, as there is limited research and development efforts in respect to new products to treat breast cancer in the African context. The development of natural, efficacious, cost effective, safer, easier accessible breast cancer management alternative is anticipated as a result of the study. Some of the compounds found in the chosen plants may be used as templates for development of new anticancer product.

Phytochemical analysis and salivary amylase inhibition activities of Carica papaya leaf and Garcinia mangostana pericarp extracts and partially purified fractions

2017 ◽  
Vol 6 (1) ◽  
pp. 34
Author(s):  
Michael Russelle Alvarez ◽  
Paolo Robert Bueno ◽  
Raymond Oliver Cruz ◽  
Richard Macapulay ◽  
Francis Jayson Vallesfin ◽  
...  
Keyword(s):  
Crude Extract ◽  
Carica Papaya ◽  
Uv Light ◽  
Digestive Enzyme ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Salivary Amylase ◽  
Garcinia Mangostana ◽  
Leaf Extracts

Plant-derived digestive enzyme inhibitors particularly those targeted to carbohydrate metabolism has been the focus of recent studies as natural supplements for weight control and diabetes. The present study explores the salivary amylase inhibition activity of Garcinia mangostana (Linn.) pericarp extracts and Carica papaya (Linn.) leaf extracts and fractions, as well as perform phytochemical screening and quantification, and thin layer – and high performance liquid chromatographic profiling. ­Results show that crude extracts and purified fractions were able to inhibit salivary amylase, with C. papaya fraction 1 being the most active at 30.89% inhibition. Phytochemical screening of all extracts tested ­positive for tannins, glycosides, phenolics, flavonoids and alkaloids. Quantification of phenolics showed that extracts contained high levels of phenolics, with C. papaya crude extract having the highest content with 219.0±12.7 mg GAE/g extract followed by G. mangostana crude extract with 247.1±18.0 mg GAE/g extract. Quantification of total flavonoids also showed C. papaya crude extract to contain the highest content with 55.12±0.679 mg QE/g extract. All extracts contained negligible alkaloid content, though. HPLC and TLC profiling showed several peaks and bands, when viewed in 210 nm and UV light, respectively. These results demonstrate in vitro the salivary amylase inhibitory activity of both plants and their potential as antidiabetic drug candidates; however, further studies need to be done, like isolation and structure elucidation of active components and toxicity assays. Keywords: Amylase inhibition, phytochemical quantification, Carica papaya, Garcinia mangostana

scholarly journals Growth and yield performances of rice (Oryza sativa var. nerica) after exposure to biosynthesized nanoparticles

2021 ◽  
Vol 45 (1) ◽  
Author(s):  
B. Ikhajiagbe ◽  
F. A. Igiebor ◽  
M. C. Ogwu
Keyword(s):  
Oryza Sativa ◽  
Moringa Oleifera ◽  
Carica Papaya ◽  
Heavy Metal Contamination ◽  
Physiological Stress ◽  
Standard Procedure ◽  
Growth And Yield ◽  
Vernonia Amygdalina ◽  
Ag Nps ◽  
Viable Seeds

Abstract Background Rice (Oryza sativa L.) is a common staple food in Nigeria. However, cultivation is impaired by heavy metal contamination, particularly iron (Fe). This study aimed to investigate the impacts of biosynthesized nanoparticles (NPs) in enhancing the growth and yield components of rice sown in ferruginous soil. Viable seeds of O. sativa var. nerica were sown in ferruginous and non-ferruginous soils. After four weeks, the plants were exposed to foliar sprays of biosynthesized NPs from silver nitrate, using extracts of leaves of Carica papaya, Vernonia amygdalina, Moringa oleifera, and Azadirachta indica; and the flowers of Hibiscus sabderiffa, following standard procedure. The originally prepared stock solution was diluted to give 5, 15, and 30% concentrations of each synthesized NP. Results Results showed that soil ferrugenicity impeded the growth and yield of rice. Azadirachta-synthesized NPs was better enhanced in the ferruginous soils, which might be due to Fe interaction and activities. Moreover, there was increased antioxidant activity in the ferruginous rice compared to the non-ferruginous rice, thus it is evidence that ferrugenicity is a major source of physiological stress for the rice plant. Conclusion The study provided evidence that Ag-NPs can enhance plant yield by huge proportions in ferruginous soil, a condition (ferrugenicity) that was hitherto inimical to yield disposition of rice.

scholarly journals Carica papaya induces in vitro thrombopoietic cytokines secretion by mesenchymal stem cells and haematopoietic cells

2015 ◽  
Vol 15 (1) ◽  
Author(s):  
Jazli Aziz ◽  
Noor Lide Abu Kassim ◽  
Noor Hayaty Abu Kasim ◽  
Nazmul Haque ◽  
Mohammad Tariqur Rahman
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Carica Papaya ◽  
Haematopoietic Cells ◽  
Cytokines Secretion

Papaya shoot tip associated endophytic bacteria isolated from in vitro cultures and host–endophyte interaction in vitro and in vivo

2007 ◽  
Vol 53 (3) ◽  
pp. 380-390 ◽  
Author(s):  
Pious Thomas ◽  
Sima Kumari ◽  
Ganiga K. Swarna ◽  
T.K.S. Gowda
Keyword(s):  
Culture Medium ◽  
Carica Papaya ◽  
In Vitro Cultures ◽  
Dependent Manner ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis ◽  
Single Organism ◽  
Dose Dependent

Fourteen distinct bacterial clones were isolated from surface-sterilized shoot tips (~1 cm) of papaya (Carica papaya L. ‘Surya’) planted on Murashige and Skoog (MS)-based papaya culture medium (23/50 nos.) during the 2–4 week period following in vitro culturing. These isolates were ascribed to six Gram-negative genera, namely Pantoea ( P. ananatis ), Enterobacter ( E. cloacae ), Brevundimonas ( B. aurantiaca ), Sphingomonas , Methylobacterium ( M. rhodesianum ), and Agrobacterium ( A. tumefaciens ) or two Gram-positive genera, Microbacterium ( M. esteraromaticum ) and Bacillus ( B. benzoevorans ) based on 16S rDNA sequence analysis. Pantoea ananatis was the most frequently isolated organism (70% of the cultures) followed by B. benzoevorans (13%), while others were isolated from single stocks. Bacteria-harboring in vitro cultures often showed a single organism. Pantoea, Enterobacter, and Agrobacterium spp. grew actively on MS-based normal papaya medium, while Microbacterium, Brevundimonas, Bacillus, Sphingomonas, and Methylobacterium spp. failed to grow in the absence of host tissue. Supplying MS medium with tissue extract enhanced the growth of all the organisms in a dose-dependent manner, indicating reliance of the endophyte on its host. Inoculation of papaya seeds with the endophytes (20 h at OD550 = 0.5) led to delayed germination or slow seedling growth initially. However, the inhibition was overcome by 3 months and the seedlings inoculated with Pantoea, Microbacterium, or Sphingomonas spp. displayed significantly better root and shoot growths.

In vitro somatic embryogenesis from suspension cultures of Carica papaya L.

2012 ◽  
Vol 136 ◽  
pp. 43-49 ◽  
Author(s):  
R. Anandan ◽  
D. Sudhakar ◽  
P. Balasubramanian ◽  
Antonia Gutiérrez-Mora
Keyword(s):  
Somatic Embryogenesis ◽  
Carica Papaya ◽  
Suspension Cultures

scholarly journals Establishment of in vitro fast-growing normal root culture of Vernonia amygdalina - a potent African medicinal plant

2009 ◽  
Vol 8 (21) ◽  
pp. 5952-5957 ◽  
Author(s):  
M Khalafalla M ◽  
M Daffalla H ◽  
A El Shemy H ◽  
Abdellatef E
Keyword(s):  
Medicinal Plant ◽  
Root Culture ◽  
Vernonia Amygdalina ◽  
Fast Growing

In Vitro Polyembryony in Carica papaya L. Ovules

1981 ◽  
Vol 104 (3) ◽  
pp. 285-288 ◽  
Author(s):  
Richard E. Litz ◽  
Robert A. Conover
Keyword(s):  
Carica Papaya

In vitro propagation of shoot buds of Carica papaya L. (caricaceae) var. Honey Dew

1990 ◽  
Vol 8 (10) ◽  
pp. 609-612 ◽  
Author(s):  
Mousumi Mondal ◽  
Sukumar Gupta ◽  
Barid Baran Mukherjee
Keyword(s):  
In Vitro Propagation ◽  
Carica Papaya ◽  
Shoot Buds

scholarly journals Optimation of In vitro Lateral Shoots Multiplication of Papaya (Carica papaya L.) “Callina” with BAP and NAA

2017 ◽  
Vol 4 (3) ◽  
pp. 102-107
Author(s):  
Darda Efendi ◽  
Mirza R Putra
Keyword(s):  
Carica Papaya ◽  
Clonal Propagation ◽  
Block Design ◽  
Shoot Multiplication ◽  
Plant Materials ◽  
Randomized Block Design ◽  
Lateral Shoots ◽  
Shoot Production ◽  
Set Up

Papaya is a popular fruit and is grown commercially in many subtropical and tropical countries. Papayas are generally grown from seeds; therefore the offsprings are not true-to-type and could come in three sexes, female, male, and hermaphrodite. Clonal propagation is required to obtain to grow true-to-type hermaphrodite papayas. In this research, we developed an in vitro protocol for shoot multiplication from lateral shoots from in vitro germinated papaya seedlings. The in vitro propagated plant materials could potentially be used as a source of papaya micro cuttings, or as scion for papaya grafting. The experiment was set up as a factorial experiment with NAA at 0, 0.1 and 0.5 mg.L-1, and BAP at 0, 0.1, 0.5, and 1.0 mg.L-1 in a completely randomized block design. BAP interacted with NAA in affecting the shoot production per explant. The optimum BAP and NAA concentration to produce lateral shoots was 0.54 mg. L-1 and 0.1 mg.L-1, respectively. Media without NAA reduced the number of lateral shoots and number of leaf per explant at any BAP concentration.Keywords: hermaphrodite, seeds, true to type, clonal propagation, micro cuttings.

Sign in / Sign up

Export Citation Format

Share Document