scholarly journals ASSOCIATION OF E-CADHERIN EXPRESSION TO CLINICAL STAGE OF NASOPHARYNGEAL CARCINOMA

2019 ◽  
Vol 1 (1) ◽  
pp. 07-10
Author(s):  
Felicia Melani Triastuti ◽  
Rizalina A. Asnir ◽  
Ashri Yudhistira ◽  
Sutoyo Eliandy
Keyword(s):  
Squamous Cell Carcinoma ◽  
Lymph Node ◽  
Nasopharyngeal Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Clinical Stage ◽  
High Rate ◽  
Mesenchymal Transition ◽  
Histopathological Type ◽  
E Cadherin

Background: Nasopharyngeal carcinoma (NPC) has a high incidence rate in southern China and Southeast Asia and a high rate of metastasis in head and neck cancer. NPC is a squamous cell carcinoma that grows from the surface of the lateral wall of the nasopharynx. E-cadherin is known as a tumor suppressor protein and the loss of this expression in tumor cells is related to epithelial-mesenchymal transition that occurs during tumor development and metastasis. Objective:To determine the relationship between E-cadherin expression and clinical stage in patients with nasopharyngeal carcinoma. Methods: An analytical study with a cross-sectional study design, the number of samples was 30 patients with NPC who underwent immunohistochemical examination on KNF paraffin block. Result: Overexpression of E-cadherin was found in T1 and T2 of 15 subjects (100%). The overexpression of E-cadherin was found in lymph node N2 and N3 of 19 subjects (95%). The overexpression of E-cadherin was found in the histopathological type of Non-Keratinizing Squamous Cell Carcinoma of 24 subjects (96%). The overexpression of E-cadherin was found at an advanced stage of 26 subjects (100%). Conclusion: No significant association was found between E-cadherin expression and tumor size (p = 1,000), lymph node metastasis (p = 1,000), histopathological type (p = 1,000) and tumor stage (p = 0.133).

Reduced E-cadherin expression is associated with lymph node metastases in laryngeal squamous cell carcinoma

2012 ◽  
Vol 39 (2) ◽  
pp. 186-192 ◽  
Author(s):  
Jing-jia Li ◽  
Ge-hua Zhang ◽  
Xin-ming Yang ◽  
Shi-sheng Li ◽  
Xian Liu ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Lymph Node ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Lymph Node Metastases ◽  
Laryngeal Squamous Cell Carcinoma ◽  
Node Metastases ◽  
E Cadherin

(S,R)3-(4-Hydroxyphenyl)-4,5-Dihydro-5-Isoxazole Acetic Acid Methyl Ester Inhibits Epithelial-to-Mesenchymal Transition through TGF-β/Smad4 Axis in Nasopharyngeal Carcinoma

2021 ◽  
Vol 21 ◽  
Author(s):  
Qibing Chen ◽  
Yan Wang ◽  
Fen Li ◽  
Xiang Cheng ◽  
Yu Xiao ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Nasopharyngeal Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Epithelial To Mesenchymal Transition ◽  
Immunofluorescence Assay ◽  
Migration And Invasion ◽  
Mesenchymal Transition

Background: Macrophage migration inhibitory factor (MIF), originally reported as an inflammation regulating molecule, is elevated in various cancer cells, which may promote carcinogenesis. Meanwhile, ISO-1 is a potent small molecular inhibitor of MIF, which has not been investigated in nasopharyngeal carcinoma (NPC); hence the impact of ISO-1 on NPC cells remains to be illustrated. Objective: This study intended to explore the biological function of ISO-1 in NPC cells in vitro and prove a possibility of ISO-1 being a novel agent in NPC treatments. Methods: Gene expression of MIF in Head and Neck squamous cell carcinoma were obtained from The Cancer Genome Atlas (TCGA) database. Nasal pharyngeal tissues were collected from adult patients undergoing nasopharyngeal biopsy for MIF level detection. Proliferation of NPC cell lines 5-8B and 6-10B was studied using Cell Counting Kit-8 (CCK-8) assay and plate-colony-formation assay, apoptosis was determined by flow cytometry and TUNEL staining, migration and invasion capacities were measured by wound-healing assay and transwell assay, all to explore the function of ISO-1 in NPC cells in vitro. Epithelial-to-mesenchymal transition (EMT) level of NPC cells was determined by Western blot analysis and immunofluorescence assay. Results: Transcript level of MIF was significantly higher in head and neck squamous cell carcinoma. Protein MIF was overexpressed in human NPC tissues compared to non-cancerous ones, and its expression could be compromised by ISO-1 in vitro. 100μM ISO-1 significantly hindered NPC cells migration and invasion capacities in vitro but acted relatively poorly on proliferation and apoptosis. Immunofluorescence assay and Western blotting implied a down-regulated EMT level through TGF-β/Smad4 axis in ISO-1 treated NPC cells compared to the vehicle. Conclusion: This study indicated that MIF antagonist ISO-1 holds impact on NPC progression by influencing the migration and invasion of NPC cells ISO-1 inhibits the EMT process of NPC cells through TGF-β/Smad4 axis, supporting that prudent application of ISO-1 may be a potential adjuvant treatment for NPC.

scholarly journals N-cadherin and hyaluronan expression in head and neck squamous cell carcinoma, relation to patient outcomes

2017 ◽  
Vol 8 (1) ◽  
pp. 19
Author(s):  
Heba A. El Hendawy ◽  
Afaf Ibrahiem ◽  
El-Nagdy SY ◽  
Zedan W
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Epithelial Mesenchymal Transition ◽  
Clinical Stage ◽  
Nodal Metastasis ◽  
Large Tumor ◽  
Mesenchymal Transition ◽  
Oral Site

Background: Epithelial-mesenchymal transition (EMT) is regarded as an essential step for tumor invasion and metastasis. In squamous cell carcinoma of head and neck (HNSCC), N-Cadherin expression and its involvement in tumor progression remains a controversial topic.Aim of the study: The present study aimed to assess the expression of N-cadherin and HA in HNSCC and further study their relation to patients survival and outcomes.Material and methods: Fifty-eight retrospective selected cases of head and neck squamous carcinomas (HNSCCs) with available paraffin blocks. Complete clinico-pathological and follow-up data were recorded. Immune staining for N-cadherin and hyaluronan were done, also, we study the correlation of the results with patients survival data.Results: Squamous cell carcinoma islands demonstrated high N-cadherin expression in 55.2% and low expression in 44.8%. N-cadherin high expression was significantly (p < .05) associated with large tumor sizes, advanced TNM clinical stage, increased incidence of recurrence and patient’s death. A significant correlation was recorded between the presence of neural invasion and N-cadherin expression (p = .004). Strong intensity of stromal HA was significantly (p < .05) associated with an oral site, nodal metastasis, and higher TNM stage. Patients with high N-cadherin expression, diffuse hyaluronan, and strong stromal hyaluronanreaction had significantly lower DFS rates (p < .05). High N-cadherin expression, diffuse hyaluronan immunoreactivity, and strong stromal hyaluronan reaction intensity had significantly lower OS rates (p < .05).Conclusion: N-cadherin and hyaluronan could be important and promising biomarkers during surveillance of patients with HNSCC.

scholarly journals Sulfiredoxin may promote metastasis and invasion of cervical squamous cell carcinoma by epithelial–mesenchymal transition

Tumor Biology ◽  
2017 ◽  
Vol 39 (3) ◽  
pp. 101042831769594
Author(s):  
Xiaoyan Chen ◽  
Kangyun Lan ◽  
Qin Liu ◽  
Xue Yang ◽  
Hui Wang
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Epithelial Mesenchymal Transition ◽  
Cervical Squamous Cell Carcinoma ◽  
Expression Level ◽  
Migration Activity ◽  
Mesenchymal Transition ◽  
Siha Cells ◽  
E Cadherin

Sulfiredoxin (Srx), a novel oxidative stress-induced antioxidant protein, has been reported to be expressed in several human tumour tissues. However, the expression and functions of Srx in cervical squamous cell carcinoma remain unknown. Here, we proved that expression of Srx was upregulated in cervical tissues as revealed by immunohistochemistry, and revealed a close correlation between the protein’s expression and the expression level of one core epithelial–mesenchymal transition marker, E-cadherin. We demonstrated that Srx was overexpressed in cervical squamous cell carcinoma and its expression level was closely correlated with lymph node metastasis and invasion of cervical squamous cell carcinoma. Meanwhile, Srx expression was negatively correlated with E-cadherin expression. The remission time (tumour-free status after surgery) of the Srx strong staining group was significantly shorter than that of the Srx weak staining group. We silenced Srx by short hairpin RNA in HeLa and SiHa cells. Diminished Srx expression upregulated E-cadherin expression. The cell invasion and migration activity in the ShSrx group were obviously decreased in HeLa and SiHa cells. Moreover, Srx regulated the expression of the other marker of epithelial–mesenchymal transition, vimentin. In conclusion, the study suggested that Srx was highly expressed in cervical squamous cell carcinoma and may promote invasion and metastasis of cervical squamous cell carcinoma via regulating epithelial–mesenchymal transition.

scholarly journals Prognostic and Risk Factors in Patients with Locally Advanced Cutaneous Squamous Cell Carcinoma of the Trunk and Extremities

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
de Lima Vazquez Vinicius ◽  
Cristovam Scapulatempo ◽  
Natalia Martins Perpetuo ◽  
Faheez Mohamed ◽  
Teóclito Sachetto de Carvalho ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Locally Advanced ◽  
Cutaneous Squamous Cell Carcinoma ◽  
Primary Tumors ◽  
Node Metastasis ◽  
E Cadherin

55 patients with advanced cutaneous squamous cell carcinoma (CSCC) of the trunk and extremities were studied.A Tissue Microarraywas constructed using immunohistochemistry to quantify expression of the HER family, E-cadherins, and podoplanin. Clinical and histopathological factors related to lymph node metastasis and prognosis were also established. Primary tumor positivity was 25.5% for EGFR, 87.3% for HER-3, and 48.1% for HER-4. Metastases were positive for EGFR in 41.7%, for HER-3 in 83.3%, and HER-4 in 43.5%. HER-2 was negative in all samples. Membrane E-cadherin and cytoplasmic E-cadherin were positive in 47.3% and 30.2% of primary tumors and 45.5% and 27.3% of metastases, respectively. Podoplanin was positive in 41.8% of primary tumors and 41.7% of metastases. Intratumoral lymphocytic infiltrate was associated with lymph node metastasis. Patients with T3 tumors had better cancer-specific survival (CSS) than those with T4 tumors; patients with no lymph node involvement had better CSS than patients with N1 tumors. Undifferentiated tumors and hyperexpression of podoplanin were negative prognostic indicators on multivariate analysis.

scholarly journals Correlation of P38 Mitogen-Activated Protein Kinase Expression to Clinical Stage in Nasopharyngeal Carcinoma

2018 ◽  
Vol 6 (11) ◽  
pp. 1982-1985 ◽  
Author(s):  
Farhat Farhat ◽  
Elvita Rahmi Daulay ◽  
Jessy Chrestella ◽  
Rizalina Arwinati Asnir ◽  
Ashri Yudhistira ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Protein Kinase ◽  
Nasopharyngeal Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Clinical Stage ◽  
Mitogen Activated Protein Kinase ◽  
Mitogen Activated Protein ◽  
Keratinizing Squamous Cell Carcinoma ◽  
Kinase Expression

BACKGROUND: Nasopharyngeal carcinoma (NPC) is uncommon and usually diagnosed at the advanced stage. A subfamily of mitogen-activated protein kinase which is called p38 mitogen-activated protein kinase (MAPK) involved in response to stress, and plays an important role in cell regulation. There is a suggestion that p38 mitogen-activated protein kinase could be a potential biomarker to determine the clinical stage of nasopharyngeal carcinoma. AIM: The aim of this study is for observing and analysing the correlation of p38 mitogen-activated protein kinase expression in regards to nasopharyngeal carcinoma patient’s clinical stage. METHODS: This study involved 126 nasopharyngeal carcinoma patients admitted to Haji Adam Malik General Hospital. RESULTS: The result of this study indicates that nasopharyngeal carcinoma mostly found in the age group 41-60 years, male, non-keratinizing squamous cell carcinoma, and stage IV group. In immunohistochemistry evaluation, most of p38 mitogen-activated protein kinase overexpressed in non-keratinizing squamous cell carcinoma, T3-T4, N2-N3 and clinical stage III-IV. Spearman’s test for categorical correlation yield p-value of < 0.001. CONCLUSION: In conclusion, there is a significant correlation between p38 mitogen-activated protein kinase expression and the clinical stage of nasopharyngeal carcinoma.

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