Association between VEGF–2578 C>A Gene Polymorphism and Lifestyle in H. Pylori Positive Gastritis Patients

Abstract Background: The polymorphisms make the differences in virulence and pathogenicity of H. pylori, which lead to different clinical manifestations after H. pylori infection. The aim research to assess correlation between between VEGF–2578 C>A Gene Polymorphism and life style in H. Pylori (+) Gastritis Patients. Method: The design of this study is a cross sectional study of H. Pylori (+) Gastritis patients, independent variables is gene polymorphism VEGF-2578C>A and dependent variables are life style (age, gender, BMI and alcohol). Infection of H. Pylori detected 14C-UBT or rapid urease test. Examination of gene polymorphism VEGF-2578C>A by performing DNA extraction (Spin Column Method) with reagent: High Pure PCR Template through PCR procedure. Results: From 59 pasien Gastritis H. Pylori (+), there is no correlation between VEGF-2578 C>A gene polymorphism (consisting of CC, CA and AA) with lifestyle (gender, age consists of < 45 years and > 45 years, BMI consists of < 25 kg/m2 and > 25 kg/m2, alcoholic drinkers ) Conclusion: There is no correlation between VEGF–2578 C>A Gene Polymorphism and life style (age, sex, BMI and alcohol drinking) in H. Pylori (+) Gastritis Patients.

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Comparison of IL-6, IL-8 Concentrations in H. pylori- and non-H. pylori-associated Gastritis

The Indonesian Biomedical Journal ◽

10.18585/inabj.v6i3.29 ◽

2014 ◽

Vol 6 (3) ◽

pp. 163 ◽

Cited By ~ 1

Author(s):

Gontar Alamsyah Siregar ◽

Sahat Salim ◽

Ricky Rivalino Sitepu

Keyword(s):

Helicobacter Pylori ◽

Serum Levels ◽

Cross Sectional Study ◽

Bivariate Analysis ◽

Rapid Urease Test ◽

Serum Samples ◽

Cross Sectional ◽

Non Invasive ◽

Urease Test ◽

H Pylori

BACKGROUND: Helicobacter pylori is a non-invasive microorganism causing intense gastric mucosal inflammatory and immune reaction. The gastric mucosal levels of the proinflammatory cytokines Interleukin 6 (IL-6) and IL-8 have been reported to be increased in H. pylori infection, but the serum levels in H. pylori infection is still controversial. The purpose of this study was to investigate the serum levels of IL-6 and IL-8 in H. pylori infection.METHODS: A cross sectional study was done on eighty consecutive gastritis patients admitted to endoscopy units at Adam Malik General Hospital and Permata Bunda Hospital, Medan, Indonesia from May-October 2014. Histopathology was performed for the diagnosis of gastritis. Rapid urease test for diagnosis of H. pylori infection. Serum samples were obtained to determine circulating IL-6 and IL-8. Univariate and bivariate analysis (independent t test) were done.RESULTS: There were 41.25% patients infected with H. pylori. Circulatory IL-6 levels were significantly higher in H. pylori-infected patients compared to H. pylori negative, but there were no differences between serum levels of IL-8 in H. pylori positive and negative patients.CONCLUSION: The immune response to H. pylori promotes systemic inflammation, which was reflected in an increased level of serum IL-6. Serum levels of IL-8 were not significantly different between H. pylori positive and negative.KEYWORDS: Helicobacter pylori, gastritis, IL-6, IL-8, cytokine

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GENDER, AGE, ENDOSCOPIC FINDINGS, UREASE AND HELICOBACTER PYLORI: ALL UNCORRELATED WITHIN A SAMPLE OF A HIGH GASTRIC CANCER PREVALENCE POPULATION IN AMAZON

Arquivos de Gastroenterologia ◽

10.1590/s0004-2803.201900000-50 ◽

2019 ◽

Vol 56 (3) ◽

pp. 264-269

Author(s):

Ariney Costa de MIRANDA ◽

Cássio CALDATO ◽

Mira Nabil SAID ◽

Caio de Souza LEVY ◽

Claudio Eduardo Corrêa TEIXEIRA ◽

...

Keyword(s):

Gastric Cancer ◽

Helicobacter Pylori ◽

Cross Sectional Study ◽

Upper Gastrointestinal Endoscopy ◽

Histopathological Analysis ◽

Rapid Urease Test ◽

Cross Sectional ◽

Cancer Prevalence ◽

Urease Test ◽

H Pylori

ABSTRACT BACKGROUND: It is widely assumed that gender, age, gastritis and Helicobacter pylori , all have some degree of correlation and, therefore, can synergistically lead to the development of gastric cancer. OBJECTIVE: In this cross-sectional study, we expected to observe the above mentioned correlation in the analysis of medical records of 67 patients of both sexes (female, n=44), mean age ± standard deviation: 41±12 years old, all from Belém (capital of Pará State, Brazilian Amazon), a city historically known as one with the highest gastric cancer prevalence in this country. METHODS: All patients were submitted to upper gastrointestinal endoscopy for gastric biopsy histopathological analysis and rapid urease test. All diagnoses of gastritis were recorded considering its topography, category and the degree of inflammatory activity, being associated or not associated with H. pylori infection. RESULTS: The results show that no statistically relevant associations were found among the prevalences of the observed variables. CONCLUSION: The authors hypothesize that observed risk factors associated to gastric cancer might be lesser synergistic than is usually expected.

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H.PYLORI INFECTION

The Professional Medical Journal ◽

10.29309/tpmj/2016.23.03.1479 ◽

2016 ◽

Vol 23 (03) ◽

pp. 307-311

Author(s):

Shabnam Naveed ◽

Syed Masroor Ahmed ◽

Zeeshan Ali ◽

Romana Awan ◽

Humaira Zakir ◽

...

Keyword(s):

Duodenal Ulcer ◽

Gastric Ulcer ◽

Cross Sectional Study ◽

Rapid Urease Test ◽

Viral Etiology ◽

Cross Sectional ◽

Urease Test ◽

Cirrhotic Patients ◽

H Pylori ◽

Study Setting

Objectives: To study the frequency of H. pylori infection and its association as acause of upper G.I bleeding in cirrhosis. Study Design: Cross Sectional Study. Setting: MedicalUnit III, JPMC Karachi. Period: January 2014 to December 2014. Methods: 100 patients ofestablished cirrhosis with upper G.I bleeding were included in this study. These patients wereevaluated for viral etiology of cirrhosis. An upper G.I Endoscopy was performed in all patientsand antral biopsies were taken. Rapid urease test was performed on biopsy specimen fordetection of H.pylori infection. Results: Out of 100 patients 71 were males and 29 were femaleswith age ranging between 14 to 70 years. Among them 53% patients turned out to be positivefor H.Pylori infection with rapid urease test. H.pylori infection was detected in 68.7% of HBsAgpositive patients and in 50% of Anti HCV positive patients. On upper G.I endoscopy 96 patientshad PHG and among them 50 (52%) were positive for H.pylori. 4 patients did not have PHGand among them 3 (75%) were positive for H.pylori. Gastric ulcer was present in 17 patientsand amongst them H.pylori was detected in 10 (58.8%) cases. Duodenal ulcer was present in 5patients and among them 2 (40%) were positive for H.pylori. Gastritis was present in 17 casesamong them 11 (64%) were positive for H.pylori. Duodenitis was present in 13 cases amongthem 11 (84.6%) patients were positive for H.pylori infection. Conclusion: The frequency ofH.pylori infection was low in cirrhotic patients. No association was seen in H.pylori infectionand causes of upper G.I bleeding in cirrhosis including PHG, gastric ulcer and duodenal ulcer

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Association between VEGF-634G>C Gene Polymorphism with Gastric Premalignant Lesions and Serum VEGF Levels in Helicobacter pylori Gastritis Patients

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2018.266 ◽

2018 ◽

Vol 6 (8) ◽

pp. 1328-1334

Author(s):

Gontar Alamsyah Siregar ◽

Ida Parwati ◽

Tri Hanggono Achmad ◽

Yoni Fuadah Syukriani

Keyword(s):

Gene Polymorphism ◽

General Hospital ◽

Serum Levels ◽

Premalignant Lesions ◽

Rapid Urease Test ◽

Serum Samples ◽

Cross Sectional ◽

Increased Risk ◽

Urease Test ◽

H Pylori

AIM: To evaluate the association between VEGF-634G>C gene polymorphism with premalignant gastric lesions as well as the level of VEGF.METHODS: This cross-sectional study included patients with H. pylori gastritis at Haji Adam Malik General Hospital, Permata Bunda General Hospital, and Universitas Sumatera Utara Hospital, Medan, Indonesia. Detection of H. pylori infection was made using positive results of 14C-UBT, rapid urease test, and/or immunohistochemistry. Gastric premalignant lesion diagnosis was made when one or more of the following were present: chronic atrophic gastritis, intestinal metaplasia, or dysplasia. Real-time polymerase chain reaction (RT-PCR) was used to examine VEGF-634G>C gene polymorphism. Additionally, serum samples of patients with H. pylori gastritis were obtained to determine the level of circulating VEGF. Data were analysed using SPSS version 22.RESULTS: A total number of 87 patients with H. pylori gastritis were included in this study. Of all participants, 26 patients (29.9%) showed gastric premalignancy. There was a significant association between GG+GC genotype of VEGF-634G>C and gastric premalignant lesions (P = 0.003; OR (CI 95%) = 6.07 (1.88-41.71)). VEGF-634 G>C polymorphism also showed an association with VEGF serum levels (P = 0.005). Patients with the GG+GC genotype would be at risk of 3.16 times to have high VEGF levels compared to CC genotypes.CONCLUSION: VEGF-634G>C polymorphism, in particular, GG+GC genotype was associated with an increased risk of gastric premalignant transformation as well as having high VEGF levels in patients with H.pylori gastritis.

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Prevalence of Helicobacter Pylori Genotypes and Their Association with Upper Gastrointestinal Diseases: A Cross Sectional Study in Southern Iran

10.21203/rs.3.rs-137967/v2 ◽

2021 ◽

Author(s):

Rahim Raufi ◽

Reza Shahriarirad ◽

SeyedehMaryam Pishva ◽

Nikta Taghipour

Keyword(s):

Helicobacter Pylori ◽

Hiatal Hernia ◽

Gastrointestinal Diseases ◽

Cross Sectional Study ◽

Rapid Urease Test ◽

Cross Sectional ◽

Southern Iran ◽

Normal Esophagus ◽

Urease Test ◽

H Pylori

Abstract Background: Investigating the prevalence of vacuolating cytotoxin (vacA), cytotoxin associated gene A (cagA), glm M genotypes, and subtypes of vacA of Helicobacter pylori (H. pylori) isolate in Jahrom, Southern Iran.Methods: DNA extracted from H. pylori samples retrieved from gastric biopsy isolated from 113 dyspeptic patients with positive rapid urease test (RUT). Genotyping was done by polymerase chain reaction (PCR) technique, using primers for vacA (s1a, s1b, s1c, s1, s2, m2, and m1), cagA, and glmM. Endoscopy was done for all the patients to screen gastrointestinal (GI) pathologies. Results: GlmM was detected in 100% of the cases. VacA subtypes s1am2, s2m2, s1am1, s1b, and s1c were detected in 27.9%, 25.6%, 22.1%, 3.5% and 2.4% of the isolates, respectively, while cagA was detected in 60.5% of the isolates. VacA alleles m1, s1, and s2 were detected in 54%, 50%, and 44% of isolates, respectively. Also, 60.5% of the isolates were cagA-vacA-positive. A significant correlation was observed between vacAs1bm1 and gastroesophageal reflux disease (GERD), and glmM positive isolates had normal esophagus. The presence of vacAs1bm1 and vacAs1bm2 has a significant association with gastric erythema. The presence of cagA showed a significant association with normal esophagus and hiatal hernia.Conclusion: In our research, the number of glmM and cagA positive isolates is higher among other genotypes, and cagA is correlated with hiatal hernia, and normal esophageal finding is correlated with glmM genotype. There was no association between the age or sex of the patients and bacterial genotype.

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A Comparative Study Among Different Invasive Methods For The Diagnosis Of Helicobacter Pylori

Faridpur Medical College Journal ◽

10.3329/fmcj.v5i1.6809 ◽

1970 ◽

Vol 5 (1) ◽

pp. 21-24

Author(s):

MDU Islam ◽

SHZ Rahman ◽

SM Shamsuzzaman ◽

N Muazzam ◽

SG Kibria ◽

...

Keyword(s):

Cross Sectional Study ◽

Rapid Urease Test ◽

World Population ◽

College Hospital ◽

Cross Sectional ◽

Female Ratio ◽

Medical College ◽

Diagnostic Endoscopy ◽

Urease Test ◽

H Pylori

H. pylori infection occurs worldwide. Approximately 50% of the world population is infected with this organism. A cross-sectional study was conducted on 81 dyspeptic patients attending at Dhaka Medical College Hospital, for diagnostic endoscopy. H. pylori infection was measured by three invasive methods: culture, rapid urease test & histopathology. Among study patients maximum (34.57%) H. pylori infected were in 21-30 years age group, bearing male female ratio 1: 92.86. Forty four (54.32%) out of 81 were culture positive, 61 (75.31%) were rapid urease test positive and 62 (76.54%) were histopathology positive. By using 'gold standard' definition, 64 (79.02%) were H. pylori infected, 17 were un-infected. Finally comparing among three invasive methods, all are highly sensitive and specific to diagnose H. pylori infection. DOI: 10.3329/fmcj.v5i1.6809Faridpur Med. Coll. J. 2010;5(1):21-24

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Utility of Rapid urease test in diagnosis of Helicobacter pylori infection

International Journal Of Medical Science And Clinical Invention ◽

10.18535/ijmsci/v6i4.05 ◽

2019 ◽

pp. 4417-4422

Author(s):

Shaan Khetrapal ◽

Samarth Shukla ◽

Safia Rana ◽

Zeeba S Jairajpuri ◽

Sourya Acharya

Keyword(s):

Helicobacter Pylori ◽

Cross Sectional Study ◽

Helicobacter Pylori Infection ◽

Rapid Urease Test ◽

Cross Sectional ◽

Intestinal Lesions ◽

Common Diagnosis ◽

Urease Test ◽

H Pylori ◽

Haematoxylin And Eosin Stain

Aim: To evaluate the role of rapid urease test in diagnosis of Helicobacter pylori infection in various gastro intestinal lesions vis a vis conventional and special staining methods. Materials & Methods: This hospital based cross sectional study was conducted on 50 cases with gastro intestinal lesions. The obtained biopsies were then subjected to rapid urease test for detection of H. pylori by using the Pronto Dry Kit immediately. Further histopathology examination using conventional H & E (haematoxylin and eosin) stain and special Gimenez stain for H. pylori was done. Results: In this study gender wise distribution of patients showed 58% males and 42% females. Chronic gastritis was found to be the most common diagnosis (28%), followed by squamous cell carcinoma (20%) chronic inflammation (16%), H. pylori gastritis and adenocarcinoma stomach (6%), adenocarcinoma esophagus, dysplasia, erosion, esophagitis and perforation in 4%. Least common diagnosis was atrophic gastritis and carcinoid in 2%. We found that 26 cases were positive for H. pylori with Rapid urease test and 24 with special stain (Gimenez). Conclusion: This study amalgamates and incorporates the valuable clinical assessment along with the endoscopic findings, the surgical pathological (histopathology) evaluation of the biopsies along with the proto dry kit (rapid urease test), as a kind of “Gastric Triple Test” for declaring the patients as positive or negative for pathogenic infection with Helicobacter pylori.

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Serum VEGF Levels in Helicobacter pylori Infection and Correlation with Helicobacter pylori cagA and vacA Genes

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2017.031 ◽

2017 ◽

Vol 5 (2) ◽

pp. 137-141

Author(s):

Gontar Siregar ◽

Dina Sari ◽

Taufik Sungkar

Keyword(s):

Helicobacter Pylori ◽

Serum Levels ◽

Cross Sectional Study ◽

Rapid Urease Test ◽

Serum Samples ◽

Cross Sectional ◽

Urease Test ◽

H Pylori ◽

Endothelial Growth Factor ◽

Vegf Level

BACKGROUND: Helicobacter pylori vacA and cagA genes are associated with higher virulence. Vascular Endothelial Growth Factor (VEGF) is one important marker for neo-angiogenesis.AIM: The purpose of this study was to investigate the relationship between VEGF serum levels with cagA and vacA genes in H. pylori infection.METHODS: A cross-sectional study was done on eighty patients that consecutive admitted to endoscopy unit. The diagnosis of H. pylori infection was based on rapid urease test. Serum samples were obtained to determine circulating VEGF level. Polymerase chain reaction was done to examine H. pylori vacA and cagA genes. Data analysis were carried-out using SPSS version 22. RESULTS: A total of 80 patients were examined. There were 45 (56.3%) patients infected with Helicobacter pylori. There were 33 (73.3%) patients with H. pylori cagA positive. Serum VEGF levels in patients with the H. pylori positive were significantly higher compared to the patients that have no H. pylori. Serum levels of VEGF were significantly higher in cagA positive than negative.CONCLUSION: Serum VEGF level is correlated with H. pylori infection and its virulence status. The more virulence of H. pylori, cagA gene, the higher serum VEGF levels were found.

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Prevalence of Idiopathic Peptic Ulcer in Southeastern Iran

Zahedan Journal of Research in Medical Sciences ◽

10.5812/zjrms.119598 ◽

2021 ◽

Vol In Press (In Press) ◽

Author(s):

Alireza Bakhshipour ◽

Raheleh Rafaiee

Keyword(s):

Peptic Ulcer ◽

Underlying Disease ◽

Cross Sectional Study ◽

Rapid Urease Test ◽

Peptic Ulcers ◽

Igg Antibody ◽

Cross Sectional ◽

Urease Test ◽

History Of ◽

H Pylori

Background: The idiopathic peptic ulcers (IPU) or non-Helicobacter pylori, non-steroidal anti-inflammatory drug non-NSAIDs associated peptic ulcers are a serious therapeutic challenge. The prevalence of IPU is increasing in some parts of the world. Objectives: This study aimed to determine the prevalence of IPU in southeastern Iran. Methods: In this cross-sectional study, a total of 367 patients with active peptic ulcer were diagnosed by endoscopy between Jan 2018 and Feb 2019. The patients were assessed for history of medication use (including NSAIDs) in the past month, smoking, alcohol, as well as a complete history of health problems related to underlying disease such as cardiopulmonary, hepatic, renal problems, and chronic pancreatitis. Biopsy samples from antrum were performed for rapid urease test (RUT), and if RUT was negative, then biopsies were sent for histopathology. If both RUT and pathologic findings were negative for H. pylori, in patients who had treatment for H. pylori eradication, anti-H. pylori IgG antibody of blood samples was assessed. Patients were considered infected with H. pylori if any of the diagnostic tests had a positive result. Results: According to the results, 336 (91.3%) cases had at least one of the two main etiologic factors (H. pylori and NSAIDs) for peptic ulcer. While 323 (87.7%) patients were H. pylori-positive, 45 (12.3%) patients were H. pylori-negative. However, out of 45 H. pylori-negative cases, 13 patients had a positive history of using NSAIDs, and 32 (8.69%) patients were IPU. Conclusions: Our study showed that both H. pylori infection and NSAIDs use remain the main cause of peptic ulcers, and the prevalence of IPU is relatively high in Zahedan.

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Serum IL-10, MMP-7, MMP-9 Levels in Helicobacter pylori Infection and Correlation with Degree of Gastritis

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2016.099 ◽

2016 ◽

Vol 4 (3) ◽

pp. 359-363 ◽

Cited By ~ 5

Author(s):

Gontar Siregar ◽

Sahat Halim ◽

Ricky Sitepu

Keyword(s):

Helicobacter Pylori ◽

Serum Levels ◽

Cross Sectional Study ◽

Mucosal Inflammation ◽

Bivariate Analysis ◽

Rapid Urease Test ◽

Serum Samples ◽

Cross Sectional ◽

Urease Test ◽

H Pylori

AIM: Helicobacter pylori causes gastric mucosal inflammation and immune reaction. However, the increase of IL-10, MMP-7, and MMP-7 levels in the serum is still controversial. The objective of this study was to investigate the serum levels of IL-10, MMP-7 & MMP-9 in gastritis patients with H. pylori infection.MATERIALS AND METHODS: A cross-sectional study was done on seventy gastritis patients that consecutive admitted to endoscopy units. The diagnosis of gastritis was made based on histopathology and diagnosis of H. pylori infection was based on rapid urease test. Serum samples were obtained to determine to circulate IL-10, MMP-7, and MMP-9 level. Univariate and bivariate analysis were done by SPSS version 22.RESULTS: Forthy percentages of the patients were infected with H. pylori. The IL-10 level was significantly higher in H. pylori-infected patients compared to non-infected patients. However, there were no differences between serum levels of MMP-7 and MMP-9 in infected and non-infected H. pylori patients.CONCLUSIONS: The immune response to H. pylori promotes systemic inflammation, which was reflected by the increased levels of serum IL-10. However, there were no significant differences in MMP-7 and MMP-9 serum levels between positive and negative infected H. pylori patients.

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