Application of image flow cytometry and photoacoustics for the characterization of red blood cell storage lesions

10.32920/ryerson.14651985 ◽

2021 ◽

Author(s):

Ruben N. Pinto

Keyword(s):

Flow Cytometry ◽

Structural Changes ◽

Blood Gas Analysis ◽

Gas Analysis ◽

In Situ Monitoring ◽

Image Flow ◽

Morphology Characterization

Significant functional/structural changes of red blood cells (RBCs) have been documented during its in vitro storage. Collectively termed as RBC storage lesions, changes include an increase in RBC oxygen saturation (SO2) and an increase in irreversibly damaged RBCs (spheroechinocytes). In this work, novel optical techniques are presented for determining the spheroechinocyte population as a function of storage time via automated image flow cytometry (IFC) morphology characterization, and the acquisition of RBC SO2 via an in situ photoacoustic (PA) method. Blood gas analysis (BGA) was used as the gold standard SO2 measure. Over the lifespan of seven blood bags, the IFC spheroechinocyte population – PA SO2 correlation was found to be strong (0.600.95) shows high potential for in situ monitoring of RBC storage lesions.

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Enlisting the Ixodes scapularis Embryonic ISE6 Cell Line to Investigate the Neuronal Basis of Tick—Pathogen Interactions

Pathogens ◽

10.3390/pathogens10010070 ◽

2021 ◽

Vol 10 (1) ◽

pp. 70

Author(s):

Lourdes Mateos-Hernández ◽

Natália Pipová ◽

Eléonore Allain ◽

Céline Henry ◽

Clotilde Rouxel ◽

...

Keyword(s):

Cell Line ◽

Peripheral Nerves ◽

Ixodes Scapularis ◽

Embryonic Cell ◽

Cell Subpopulation ◽

In Vivo Experiments

Neuropeptides are small signaling molecules expressed in the tick central nervous system, i.e., the synganglion. The neuronal-like Ixodes scapularis embryonic cell line, ISE6, is an effective tool frequently used for examining tick–pathogen interactions. We detected 37 neuropeptide transcripts in the I. scapularis ISE6 cell line using in silico methods, and six of these neuropeptide genes were used for experimental validation. Among these six neuropeptide genes, the tachykinin-related peptide (TRP) of ISE6 cells varied in transcript expression depending on the infection strain of the tick-borne pathogen, Anaplasma phagocytophilum. The immunocytochemistry of TRP revealed cytoplasmic expression in a prominent ISE6 cell subpopulation. The presence of TRP was also confirmed in A. phagocytophilum-infected ISE6 cells. The in situ hybridization and immunohistochemistry of TRP of I. scapularis synganglion revealed expression in distinct neuronal cells. In addition, TRP immunoreaction was detected in axons exiting the synganglion via peripheral nerves as well as in hemal nerve-associated lateral segmental organs. The characterization of a complete Ixodes neuropeptidome in ISE6 cells may serve as an effective in vitro tool to study how tick-borne pathogens interact with synganglion components that are vital to tick physiology. Therefore, our current study is a potential stepping stone for in vivo experiments to further examine the neuronal basis of tick–pathogen interactions.

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Non-Polarographic Blood Gas Analysis I. in Vitro Evaluation of Gas Chromatograph System

Biomaterials Medical Devices and Artificial Organs ◽

10.3109/10731197609118661 ◽

1976 ◽

Vol 4 (3-4) ◽

pp. 385-396 ◽

Cited By ~ 3

Author(s):

Thomas A. Massaro ◽

Jean Behrens-Tepper ◽

Stuart J. Updike

Keyword(s):

Blood Gas Analysis ◽

Gas Analysis ◽

Blood Gas ◽

Gas Chromatograph ◽

In Vitro Evaluation

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An instrumented sample holder for time-lapse microtomography measurements of snow under advective airflow

Geoscientific Instrumentation Methods and Data Systems ◽

10.5194/gi-3-179-2014 ◽

2014 ◽

Vol 3 (2) ◽

pp. 179-185 ◽

Cited By ~ 7

Author(s):

P. P. Ebner ◽

S. A. Grimm ◽

M. Schneebeli ◽

A. Steinfeld

Keyword(s):

Structural Changes ◽

Time Lapse ◽

Temperature Gradients ◽

Sample Holder ◽

Experimental Characterization ◽

Computational Fluid Dynamics Simulations ◽

Dynamics Simulations ◽

Snow Samples

Abstract. An instrumented sample holder was developed for time-lapse microtomography of snow samples to enable in situ nondestructive spatial and temporal measurements under controlled advective airflows, temperature gradients, and air humidities. The design was aided by computational fluid dynamics simulations to evaluate the airflow uniformity across the snow sample. Morphological and mass transport properties were evaluated during a 4-day test run. This instrument allows the experimental characterization of metamorphism of snow undergoing structural changes with time.

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Photoreflectance Characterization of the Semi-Insulating InP Substrate Interface with InGaAs and InAlAs Epilayers

MRS Proceedings ◽

10.1557/proc-281-121 ◽

1992 ◽

Vol 281 ◽

Author(s):

Weimin Zhou ◽

H. Shen ◽

J. Pamulapati ◽

M. Dutta ◽

B. R. Bennett ◽

...

Keyword(s):

Electron Transition ◽

In Situ Monitoring ◽

Signal Phase ◽

Mbe Growth ◽

Substrate Interface ◽

Inp Substrate ◽

Excitonic Transition ◽

Inp Substrates

ABSTRACTPhotoreflectance (PR) has been performed on a series of undoped and n-type, InGaAs and InAlAs molecular beam epitaxy (MBE) grown layers with different In mole fractions, and epilayer thicknesses on Fe-doped semi-insulating (SI)-InP substrates. From investigations of the temperature dependence, time constant dependence and an additional cw light beam intensity dependence, three substrate peaks are identified as an excitonic transition from the substrate, a free electron transition near the interface which gives a Franz-Keldysh oscillation (KFO), and a transition from the spin-orbit split-off valence band. The results are indicative of a redistribution of charge near the substrate interface in the process of MBE growth; the associated PR signal (phase) could be used for in-situ monitoring of epilayer growth on SI-InP wafers.

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