scholarly journals Diagnostic and prognostic value of immune indicators of inflammation in the course and progression of gastroesophageal reflux disease and autoimmune thyroiditis in young people

2019 ◽  
Vol 45 (4) ◽  
pp. 54-59
Author(s):  
T. M. Pasiieshvili
Keyword(s):  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Inflammatory Reaction ◽  
Autoimmune Thyroiditis ◽  
Reflux Disease ◽  
Young Patients ◽  
Thyroid Stimulating Hormone ◽  
Endocrine Effect ◽  
Tnf Α ◽  
Wide Range

Cytokines are considered as “microendocrine system” due to the fact that they have a triple mechanism of action. First, they affect the cell-producer (autocrine effect); secondly, they influence neighboring (adjacent) cells (paracrine effect); thirdly, they affect the distant cells of organs and tissues (endocrine effect). Most cytokines have a wide range of biological activity, which is associated with their synthesis and secretion of various types of cells. Aim of study was to determine the role and prognostic values of tumor necrosis factor (TNF) α, interleukin (IL) 1β and IL-18 in young patients with autoimmune thyroiditis (AIT) and gastroesophageal reflux disease (GERD). Materials and methods. Study involved 83 patients (students) with GERD and AIT. The average age for the group was 22.1±2.11 years. The comparison group was represented by 30 patients of similar age with isolated GERD. The enzyme immunoassay was used to study the content of TNF-α, IL-1β and IL-18. Results. Upon evaluating the complaints of patients, it was found that the main manifestation of GERD was heartburn of varying severity, duration and frequency of occurrence. It has been shown that the course of GERD occurs against the background of the development of a systemic inflammatory reaction (increased synthesis of proinflammatory cytokines IL-1β and TNF-α), and also depends on the form of esophagitis: more pronounced in the erosive variant of the mucous membrane of the esophagus. At the same time, there was an increase in the synthesis of IL-18, which, with isolated GERD, is associated with a general inflammatory reaction, and with the addition of AIT, the inclusion of immune mechanisms. Relationship between the level of TNF-α in peripheral blood and the content of thyroid-stimulating hormone was studied. However, unlike the results of other scientists, the authors have not identified such a relationship.

scholarly journals CASPASE-DEPENDENT ACTIVATION OF CASCADE CYTOKINES IN YOUNG PATIENTS WITH AUTOIMMUNE THYROIDITIS IN COMBINATION WITH GASTROESOPHAGEAL REFLUX DISEASE

2021 ◽  
pp. 58-63
Author(s):  
Tamara Pasiieshvili ◽  
Lyudmila Pasiieshvili ◽  
Natalia Zhelezniakova ◽  
Olga Kovalyova
Keyword(s):  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Autoimmune Thyroiditis ◽  
Reflux Disease ◽  
Young Patients ◽  
Prognostic Indicator ◽  
Esophageal Mucosa ◽  
Caspase 1 ◽  
Local Methods ◽  
Commercial Kits

The aim of the study: to assess the role of caspase-1 and IL-18 in the implementation of the inflammatory response in young patients with autoimmune thyroiditis (AIT) associated with gastroesophageal reflux disease (GERD). Materials and methods. The study was conducted in two groups of patients homogeneous by gender and age. The first group included 42 patients with an isolated AIT and the second group – 120 patients with a comorbid course of AIT and GERD. The contingent of the surveyed was students. Median age was from 18 to 25 years: 23.1±1.2 years in group with isolated AIT and 21.9±2.7 years in group with combined pathology. The activity of caspase-1 was determined using “Elabscience” kits, USA (China) by immunoassay method. The content of IL-18 was investigated using commercial kits “Bender MedSystems GmbH” (Austria) according to the proposed methods by immunoassay method. Statistical data processing was carried out using the local universal software package Statistica Basic Academic 13 for Windows En Local. Methods of non-parametric statistics were used: Kruskal-Wallis test, median test, Mann-Whitney test. Results. It has been found that caspase-1 is activated in patients both in the isolated course of AIT and in its combination with GERD. At the same time, a significant increasing in the synthesis of IL-18 was established, which has pro-inflammatory and autoimmune effects. Re-examination after 2 months of caspase-1 and IL-18 in examined persons determined decreasing their levels, but control results were not achieved. Conclusion. In young patients, the combination of GERD and AIT, as well as an isolated AIT is accompanied by an increasing the levels of caspase-1 and IL-18 in blood serum related to inflammatory process in the thyroid gland and esophageal mucosa with autoimmune component. These biomarkers may reflect the severity of clinical course of diseases and serve as prognostic indicator of outcome in case of comorbidity.

scholarly journals Gastroesophageal reflux disease and autoimmune thyroiditis: features of pathomorphological manifestation in young people

2021 ◽  
pp. 4-8
Author(s):  
Tamara Pasiieshvili ◽  
Natalia Zhelezniakova ◽  
Tetiana Bocharova ◽  
Lyudmila Pasiyeshvili
Keyword(s):  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Autoimmune Thyroiditis ◽  
Reflux Disease ◽  
Intercellular Space ◽  
Basal Layer ◽  
Young Patients ◽  
Esophageal Mucosa ◽  
Leukocyte Infiltration ◽  
Morphological Form

The aim of the work. To study the effect of concomitant autoimmune thyroiditis (AIT) on the pathomorphological features of lesions of the esophageal mucosa in young patients with gastroesophageal reflux disease (GERD). Material and research methods. The study included 165 individuals. The contingent of the surveyed was students of Kharkov higher educational institutions. The main group consisted of 120 patients with a combined course of GERD and AIT, the comparison group included 65 individuals with an isolated GERD. The morphological form of the GERD was revealed during esophagogastroduodenoscopy (“Fuginon” system). A histomorphological study of the obtained biopsy material from the mucous membrane of the esophagus was carried out. Samples were studied on an Olympus BX-41 microscope. Morphometric study of the esophageal mucosa was performed using the Olympus DP-Soft. Research results. Histological examination of biopsy specimens revealed that the main pathomorphological signs of GERD in both groups were hyperplasia of the basal zone, lengthening of epithelial papillae, leukocyte infiltration, intercellular edema, expansion of the intercellular space, dystrophic changes, submucous fibrosis, the presence of severe inflammatory infiltration in the submucosal layer. Presence of concomitant AIT was associated with a statistically higher frequency of occurrence of certain signs: hyperplasia of the basal layer of the epithelium, elongation of the papillae, epithelial edema, expansion of the intercellular space, dystrophic changes in the epithelium (p<0.05). Conclusions. The presence of concomitant AIT in young patients with GERD does not affect the incidence of erosive GERD, but is associated with a significant increase in the severity of erosive esophagitis. The comorbid course of GERD and AIT in the student population is accompanied by a significant increase in the incidence and statistically significant intensification of the severity of hyperplasia of the basal layer of the epithelium, elongation of connective tissue papillae and leukocyte infiltration compared with isolated GERD

scholarly journals TOTAL ANTIOXIDANT ACTIVITY AND INFLAMMATION MARKERS DURING PROGRESSION OF GASTROESOPHAGEAL REFLUX DISEASE IN YOUNG PATIENTS WITH AUTOIMMUNE THYROIDITIS

2021 ◽  
Vol 21 (1) ◽  
pp. 44-48
Author(s):  
T.M. Pasiieshvili
Keyword(s):  
Antioxidant Activity ◽  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Autoimmune Thyroiditis ◽  
Reflux Disease ◽  
Young Patients ◽  
Total Antioxidant Activity ◽  
Histological Changes ◽  
Oesophageal Mucosa ◽  
Total Antioxidant

The aim of this study is to establish the content of inflammation markers (tumour necrosis factor alpha and interleukin 18) and the index of total antioxidant activity in young patients with comorbidity between gastroesophageal reflux disease and autoimmune thyroiditis and to determine their dependence on morpho-histological changes in the oesophageal mucosa. Materials and methods. The study involved 182 students aged 18 to 25 years: 120 patients with comorbidity between gastroesophageal reflux disease and autoimmune thyroiditis, 42 those having an isolated course of autoimmune thyroiditis. The methodology included complex clinical, instrumental, immunological, statistical methods. Results. Determining the activity of proinflammatory cytokines has revealed their statistically significant increase. The level of these cytokines depends on morpho-histological changes in the mucous membrane and reaches the maximum during the erosive process. At the same time, the indicator of total antioxidant activity significantly decreases. In this case, the erosive form of the disease is accompanied by more expressive changes in this indicator. Conclusions. The inflammatory process in the oesophagus is accompanied by the activation of the pro-inflammatory segment of immunity against the background of the suppression of the antioxidant system, which is dependent on morphological changes in the oesophageal mucosa. These changes will contribute to the development of pathological apoptosis and the progression of nosologies.

scholarly journals VEGETATIVE REACTIVITY AND VEGETATIVE SUPPLY IN DIFFERENT VARIANTS OF GASTROESOPHAGEAL REFLUX DISEASE IN YOUNG PATIENTS WITH AUTOIMMUNE INFLAMMATION

2020 ◽  
Vol 20 (1) ◽  
pp. 115-119
Author(s):  
T.M. Pasiieshvili
Keyword(s):  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Mucous Membrane ◽  
Autonomic Dysfunction ◽  
Autoimmune Thyroiditis ◽  
Reflux Disease ◽  
Young Patients ◽  
Clinical Manifestations ◽  
Autonomic Reactivity ◽  
Almost All

Aim: to determine the state and role of autonomic reactivity and autonomic supply in patients with different manifestations of gastroesophageal reflux disease in combination with autoimmune thyroiditis through psychological testing by using a questionnaire of A.M. Vejn. Materials and methods. 87 patients with gastroesophageal reflux disease and autoimmune thyroiditis and 45 persons with an isolated gastroesophageal reflux disease were enrolled in the study. All the examined individuals were students. Results. It has been shown that the course of diseases is accompanied by a layering of clinical manifestations of autonomic dysfunction, which are more pronounced in cases of comorbidities. It has been found out that vegetative supply prevails in a number of indicators in the patients with an erosive form of gastroesophageal reflux disease. It has been proven that in almost all the cases of erosion (8 people - 72.7%), the individuals experienced some clinical manifestations of vegetative provision, such as general weakness, increased fatigue, lability of the pulse and blood pressure, and palpitation. At the same time, the “vegetative tail” was recorded only in a third (34.7%) of the studied without erosive lesions of the esophageal mucous membrane. The patients with an erosive form of the disease have a long period of experiencing some manifestations of autonomic dysfunction (up to 3 months) that considerably affects the quality of their life. Conclusions. Coupling of autoimmune thyroiditis and gastroesophageal reflux disease leads to the emergence of autonomic reactivity. Vegetative “supply” of the course of comorbidity between gastroesophageal reflux disease and autoimmune thyroiditis becomes more pronounced in the presence of an erosive process in the mucous membrane, which is also known as one of the factors of its possible manifestations.

scholarly journals Gene Expression Profiling of Inflammatory Cytokines in Esophageal Biopsies of Different Phenotypes of Gastroesophageal Reflux Disease: a cross-sectional study.

2020 ◽  
Author(s):  
Monica Zavala-Solares ◽  
Gabriela Fonseca-Camarillo ◽  
Miguel Valdovinos ◽  
Julio Granados ◽  
Guido Grajales-Figueroa ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Reflux Disease ◽  
Cross Sectional Study ◽  
Control Group ◽  
Esophageal Mucosa ◽  
Cross Sectional ◽  
Tnf Α ◽  
And Control

Abstract Background: Patients clinical endoscopic phenotypes in gastroesophageal reflux disease (GERD) are classified as: Barrett's esophagus (BE), erosive esophagitis (EE) and non-erosive gastroesophageal reflux disease (NERD). NERD are subclassified in Abnormal acid exposure (AAE) and Normal acid exposure (NAE) according to pH monitoring study. The aim of this study was to characterize genes involved in the pathophysiology and immune response of GERD.Methods: This is an observational and cross-sectional study. All patients with BE, EE, AAE, NAE and control group were subjected to a superior endoscopy (with biopsies of esophageal mucosa). The cytokine mRNA relative quantification of target genes was conducted by RT-PCR. Changes in gene expression were assessed of the genes associated with inflammation in each disease phenotype. Statistical analysis of differential gene expression was performed by using Dunn's Multiple Comparison non-parametric test. A p value < 0.05 was considered as significant. Results: A total of 82 patients were included and they were divided into the following groups: Group BE 16 (19.51%), Group EE 23 (28.04%), Group AAE 13 (15.86%), NAE (15.86%) and Control Group 17 (20.73%). When comparing with control group we found: patients with BE showed an increased expression of IL-8 (P<0.005) and higher levels of: IL-10 and MMP-3, MMP-9 as well; patients with EE had higher levels of IL-1B, IL-6 and IL-10 (P<0.005), patients with AAE showed an increased expression of Il-1B, Il-6, IFN-γ and TNF-α (P<0.005). AAE had a higher expression of Il-1B and TNF-α than NAE (P<0.005). Conclusions: This study demonstrates the differential expression of mediators of inflammation in the esophageal mucosa of patients in GERD endoscopic phenotypes. MMP3 could be implicated in damage to esophageal mucosa. IL-1B and TNF-α could be a differential diagnosis between AAE and NAE in the non-erosive phenotype from endoscopic biopsies.

scholarly journals ACTIVITY OF MITOCHONDRIAL ANTIOXIDANT DEFENSE SYSTEM IN YOUNG PATIENTS WITH GASTROESOPHAGEAL REFLUX DISEASE

2020 ◽  
Vol 7 (4) ◽  
pp. 164-167
Author(s):  
Olga Kovalyova ◽  
Tamara Pasiieshvili
Keyword(s):  
Superoxide Dismutase ◽  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Reflux Disease ◽  
Manganese Superoxide Dismutase ◽  
Antioxidant Defense ◽  
Young Patients ◽  
Antioxidant Defense System ◽  
Defense System ◽  
Manganese Superoxide

Background. Despite numerous studies, the pathogenesis of gastroesophageal reflux disease remains unclear. Aim of research: assessment the activity of antioxidant defense system in young patients with GERD based on expression of biomarker associated with mitochondrial function. Material and methods. The study included 45 patients with gastroesophageal reflux disease. The examined contingent was presented by students age from 18 to 25 years. 20 healthy persons were included as control group. Levels of manganese superoxide dismutase were determined in blood serum of study persons with enzyme immunoassays (ELISA, Elabscience, USA). Statistical data processing by the Statistica Basic Academic 13 for Windows En local was made. Results. Gastroesophageal reflux disease in young patients is characterized by significantly increasing of manganese superoxide dismutase as compare to control group (7.1700 ng/ml vs 4.4720 ng/ml respectively, p<0.01). Presence of erosion in esophagus mucous doesn't accompanied by significant changes of evaluated parameter as compare with non-erosion form of disease in patients. Conclusion. The elevation in young patients with GERD the biomarker of mitohondrial antioxidant defense system we may speculate as adaptive response contributing to non-specific citoprotection. Taking to account the publishing facts about dual role of manganese superoxide dismutase it is necessary to monitoring antioxidant enzyme in patients with gastroesophageal reflux disease for prediction of possible complications and outcome.

scholarly journals The features of psycho-vegetative disorders and their role in the pathogenesis of gastroesophageal reflux disease with obesity

2015 ◽  
Vol 14 (2) ◽  
pp. 142-145
Author(s):  
Alex Oparin ◽  
Daria Korniienko ◽  
Anatoliy Oparin ◽  
Nataly Lavrova ◽  
Julia Dvoyashkina ◽  
...  
Keyword(s):  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Reflux Disease ◽  
Vegetative State ◽  
Medical Science ◽  
Young Patients ◽  
Developed Countries ◽  
Esophageal Wall ◽  
Significant Difference ◽  
The Developed Countries

Background: Gastroesophageal reflux disease (GERD) is a disease of the XXI century, affecting the population in the developed countries. There is a clear tendency to increasing of gastroesophageal reflux disease among people of young age.Aim: The aim of the study is to determine the characteristics and role of psychosomatic and vegetative state disorders in the pathogenesis of GERD with concomitant obesity.Materials and methods: 50 young patients with GERD were examined during the study. Depending on the presence or absence of concomitant obesity, two groups were formed. They underwent pH-metery, ultrasonic scanning. State of psycho-somatic, vegetative statuses and quality of life we determined by the questionnaires of Beck, Spielberg, Sheehan, Wayne.Results: During the ultrasound examination we determined the esophageal opening diameter, the thickness of esophageal wall, the esophagus width in the lower one third of the esophagus differed significantly in 2 groups of patients (p<0.05). In the group of the GERD patients with concomitant obesity the sharp increase of depression level was found, comparing with both the standard and the second group (p<0.05). It wasn’t found the significant difference amount other figures of vegetative and psychosomatic statuses in 2 groups of patients (p>0.05). The patients with GERD of both groups had significantly higher level of motor-evacuation and psycho-vegetative disorders comparing with healthy people (p<0.05).Conclusion: GERD patients have pronounced motor-evacuation disorders and significantly greater manifestations of psycho-vegetative disorders which were more pronounced in gastroesophageal reflux disease patients with concomitant obesity.Bangladesh Journal of Medical Science Vol.14(2) 2015 p.142-145

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