Identification of Structurally Similar Phytochemicals to Quercetin with High SIRT1 Binding Affinity and Improving Diabetic Wound Healing by Using In silico Approaches

Diabetes Mellitus is the most prevalent metabolic disorder that is increasing at an alarming rate worldwide. The unregulated glucose level leads to various types of health disorders, and one of the major diabetic complications is delayed wound healing. Due to the more side effects of synthetic drugs, there is a need to explore plants and their phytochemicals for medicinal purposes. It was found that Quercetin, a flavonoid, increases the rate of diabetic wound healing by enhancing the expression of SIRT1. This demands more insight towards Quercetin and its similar compounds, as it is hypothesized that similar compounds may have similar biological properties. Thus similarity searching was done to identify the most similar compounds of Quercetin, and then the molecular docking of the screened compounds was performed using AutoDock Vina. The unique ligands were docked into the active site of SIRT1 protein (PDB ID: 4ZZJ). The binding free energy of the interacting ligand with the protein was estimated. Six compounds were identified which possess the maximum structural similarity with Quercetin, and upon docking, it was found that gossypetin and herbacetin have similar binding modes and binding energy as that of Quercetin (-7.5 kcal/mol). Therefore, the hypothesis has been validated by in silico analysis. Our study identified two phytochemicals, Gossypetin, and Herbacetin which can prove beneficial for improving diabetic wound healing but needs to be validated further by in vitro and in vivo studies.

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Synergistic Effects of Photobiomodulation Therapy with Combined Wavelength on Diabetic Wound Healing In Vitro and In Vivo

Photobiomodulation Photomedicine and Laser Surgery ◽

10.1089/photob.2021.0068 ◽

2021 ◽

Author(s):

Weijie Cai ◽

Musha Hamushan ◽

Yubo Zhang ◽

Zhengyu Xu ◽

Zun Ren ◽

...

Keyword(s):

Wound Healing ◽

Synergistic Effects ◽

Photobiomodulation Therapy ◽

Diabetic Wound ◽

Diabetic Wound Healing

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A Comprehensive Review on Physiological Effects of Curcumin

Drug Research ◽

10.1055/a-1207-9469 ◽

2020 ◽

Vol 70 (10) ◽

pp. 441-447

Author(s):

Rabiya Ahsan ◽

Md Arshad ◽

Mohammad Khushtar ◽

Mohd Afroz Ahmad ◽

Mohammad Muazzam ◽

...

Keyword(s):

Wound Healing ◽

Curcuma Longa ◽

Biological Properties ◽

In Vivo Studies ◽

Intracellular Signalling Pathways ◽

Excellent Safety Profile ◽

Cardiac Disorders ◽

Indian Food

AbstractTurmeric (Curcuma longa Linn) is an herbal medicine which is traditionally used as a spice, food colouring or flavouring agent and widely used for several diseases such as biliary disorders, cough, hepatic disorders, rheumatism, wound healing, sinusitis, diabetes, cardiac disorders and neurological disorder. It belongs to the Zingiberaceae family. Turmeric is a popular domicile remedy used in Indian food, is mainly a native of south-east Asia, is widely cultivated in India, Sri Lanka, Indonesia, China, Jamaica , Peru, Haiti and Taiwan and it is very less expensive. Curcumin is the main principle of turmeric. Curcumin has shown various biological properties pre-clinically and clinically. Curcumin is a highly pleiotropic molecule which can be modulators of various intracellular signalling pathways that maintain cell growth. It has been reported as anti-inflammatory, anti-angiogenic, antioxidant, wound healing, anti-cancer, anti-Alzheimer and anti-arthritis and possesses an excellent safety profile. All previous review articles on curcumin have collected the biological/pharmacological activities but this review article summarises the most interesting in vitro and in vivo studies of curcumin on most running diseases around the whole world.

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Shedding Light on a New Treatment for Diabetic Wound Healing: A Review on Phototherapy

The Scientific World JOURNAL ◽

10.1155/2014/398412 ◽

2014 ◽

Vol 2014 ◽

pp. 1-13 ◽

Cited By ~ 39

Author(s):

Nicolette N. Houreld

Keyword(s):

Wound Healing ◽

Laser Irradiation ◽

Diabetic Wound ◽

Impaired Wound Healing ◽

Diabetic Wound Healing ◽

New Treatment ◽

Underlying Mechanisms ◽

Intensity Laser

Impaired wound healing is a common complication associated with diabetes with complex pathophysiological underlying mechanisms and often necessitates amputation. With the advancement in laser technology, irradiation of these wounds with low-intensity laser irradiation (LILI) or phototherapy, has shown a vast improvement in wound healing. At the correct laser parameters, LILI has shown to increase migration, viability, and proliferation of diabetic cellsin vitro; there is a stimulatory effect on the mitochondria with a resulting increase in adenosine triphosphate (ATP). In addition, LILI also has an anti-inflammatory and protective effect on these cells. In light of the ever present threat of diabetic foot ulcers, infection, and amputation, new improved therapies and the fortification of wound healing research deserves better prioritization. In this review we look at the complications associated with diabetic wound healing and the effect of laser irradiation bothin vitroandin vivoin diabetic wound healing.

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Simvastatin nanoparticles loaded polymeric film as a potential strategy for diabetic wound healing: in vitro and in vivo evaluation

Current Drug Delivery ◽

10.2174/1567201818666210720150929 ◽

2021 ◽

Vol 18 ◽

Author(s):

Saima Tufail ◽

Muhammad Irfan Siddique ◽

Muhammad Sarfraz ◽

Muhammad Farhan Sohail ◽

Muhammad Nabeel Shahid ◽

...

Keyword(s):

Wound Healing ◽

Healing Process ◽

Chitosan Nanoparticles ◽

Albino Rats ◽

Wound Healing Process ◽

Diabetic Wound ◽

In Vivo Evaluation ◽

Diabetic Wound Healing

Introduction: The pleiotropic effects of statins are recently explored for wound healing through angiogenesis and lymph-angiogenesis that could be of great importance in diabetic wounds. Aim: Aim of the present study is to fabricate nanofilm embedded with simvastatin loaded chitosan nanoparticles (CS-SIM-NPs) has been reported herein to explore the efficacy of SIM in diabetic wound healing. Methods: The NPs, prepared via ionic gelation, were 173nm ± 2.645 in size with a zeta potential -0.299 ± 0.009 and PDI 0.051 ± 0.088 with excellent encapsulation efficiency (99.97%). The optimized formulation (CS: TPP, 1:1) that exhibited the highest drug release (91.64%) was incorporated into polymeric nanofilm (HPMC, Sodium alginate, PVA), followed by in vitro characterization. The optimized nanofilm was applied to the wound created on the back of diabetes-induced (with alloxan injection 120 mg/kg) albino rats. Results: The results showed significant (p < 0.05) improvement in the wound healing process compared to the diabetes-induced non-treated group. The results highlighted the importance of nanofilms loaded with SIM-NPs in diabetic wound healing through angiogenesis promotion at the wound site. Conclusion: Thus, CS-SIM-NPs loaded polymeric nanofilms could be an emerging diabetic wound healing agent in the industry of nanomedicines.

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Nanofiber/hydrogel Core-shell Scaffolds With Three-dimensional Multilayer Patterned Structure for Accelerating Diabetic Wound Healing

10.21203/rs.3.rs-952440/v1 ◽

2021 ◽

Author(s):

Jiankai Li ◽

Tianshuai Zhang ◽

Mingmang Pan ◽

Feng Xue ◽

Fang Lv ◽

...

Keyword(s):

Wound Healing ◽

Three Dimensional ◽

Vapor Permeability ◽

Core Shell ◽

Diabetic Wound ◽

3D Network ◽

Diabetic Wound Healing ◽

Diabetic Wounds

Abstract Impaired angiogenesis is one of the predominant reasons for non-healing diabetic wounds. Herein, a nanofiber/ hydrogel core-shell scaffold with three-dimensional (3D) multilayer patterned structure (3D-PT-P/GM) was introduced for promoting diabetic wound healing with improved angiogenesis. The results showed that the 3D-PT-P/GM scaffolds possessed multilayered structure with interlayer spacing of about 15-80 μm, and the hexagonal micropatterned structures were uniformly distributed on the surface of each layer. The nanofibers in the scaffold exhibited distinct core-shell structures with Gelatin methacryloyl (GelMA) hydrogel as the shell and Poly (D, L-lactic acid) (PDLLA) as the core. The results showed that the porosity, water retention time and water vapor permeability of the 3D-PT-P/GM scaffolds increased to 1.6 times, 21 times, and 1.9 times than that of the two-dimensional (2D) PDLLA nanofibrous scaffolds, respectively. The in vitro studies showed that the 3D-PT-P/GM scaffolds could significantly promote cell adhesion, proliferation, infiltration and migration throughout the scaffolds, and the expression of cellular communication protein-related genes, as well as angiogenesis-related genes in the same group, was remarkably upregulated. The in vivo results further demonstrated that the 3D-PT-P/GM scaffolds could not only effectively absorb exudate and provide a moist environment for the wound sites, but also significantly promote the formation of a 3D network of capillaries. As a result, the healing of diabetic wounds was accelerated with enhanced angiogenesis, granulation tissue formation, and collagen deposition. These results indicate that nanofiber/ hydrogel core-shell scaffolds with 3D multilayer patterned structures could provide a new strategy for facilitating chronic wound healing.

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Biological properties of terpinolene evidenced by in silico, in vitro and in vivo studies: A systematic review

Phytomedicine ◽

10.1016/j.phymed.2021.153768 ◽

2021 ◽

Vol 93 ◽

pp. 153768

Author(s):

Isis Oliveira Menezes ◽

Jackelyne Roberta Scherf ◽

Anita Oliveira Brito Pereira Bezerra Martins ◽

Andreza Guedes Barbosa Ramos ◽

Jullyana de Souza Siqueira Quintans ◽

...

Keyword(s):

Systematic Review ◽

In Silico ◽

Biological Properties ◽

In Vivo Studies

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Nanotechnology-based Therapeutic Applications: In Vitro, In Vivo Clinical Studies for Diabetic Wound Healing

Biomaterials Science ◽

10.1039/d1bm01211h ◽

2021 ◽

Author(s):

Sheikh Tanzina Haque ◽

Subbroto Kumar Saha ◽

Md. Enamul Haque ◽

Nirupam Biswas

Keyword(s):

Wound Healing ◽

Side Effects ◽

Conventional Treatment ◽

Treatment Modalities ◽

Diabetic Wound ◽

Chronic Complications ◽

Diabetic Wound Healing ◽

Diabetic Wounds

Diabetic wounds often presage chronic complications that are difficult to treat. Unfortunately, existing conventional treatment modalities often warrant unpremeditated side effects, given the need to develop alternative therapeutic phenotypes that...

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Fibrin Glue Enhances Adipose-Derived Stromal Cell Cytokine Secretion and Survival Conferring Accelerated Diabetic Wound Healing

Stem Cells International ◽

10.1155/2018/1353085 ◽

2018 ◽

Vol 2018 ◽

pp. 1-8 ◽

Cited By ~ 2

Author(s):

Ursula Hopfner ◽

Matthias M. Aitzetmueller ◽

Philipp Neßbach ◽

Michael S. Hu ◽

Hans-Guenther Machens ◽

...

Keyword(s):

Wound Healing ◽

Fibrin Glue ◽

Stromal Cell ◽

Wound Closure ◽

Chronic Wounds ◽

Imaging System ◽

Diabetic Wound ◽

Diabetic Wound Healing

Introduction. Although chronic wounds are a major personal and economic burden, treatment options are still limited. Among those options, adipose-derived stromal cell- (ASC-) based therapies rank as a promising approach but are restricted by the harsh wound environment. Here we use a commercially available fibrin glue to provide a deliverable niche for ASCs in chronic wounds. Material and Methods. To investigate the in vitro effect of fibrin glue, cultivation experiments were performed and key cytokines for regeneration were quantified. By using an established murine chronic diabetic wound-healing model, we evaluated the influence of fibrin glue spray seeding on cell survival (In Vivo Imaging System, IVIS), wound healing (wound closure kinetics), and neovascularization of healed wounds (CD31 immunohistochemistry). Results. Fibrin glue seeding leads to a significantly enhanced secretion of key cytokines (SDF-1, bFGF, and MMP-2) of human ASCs in vitro. IVIS imaging showed a significantly prolonged murine ASC survival in diabetic wounds and significantly accelerated complete wound closure in the fibrin glue seeded group. CD31 immunohistochemistry revealed significantly more neovascularization in healed wounds treated with ASCs spray seeded in fibrin glue vs. ASC injected into the wound bed. Conclusion. Although several vehicles have shown to successfully act as cell carrier systems in preclinical trials, regulatory issues have prohibited clinical usage for chronic wounds. By demonstrating the ability of fibrin glue to act as a carrier vehicle for ASCs, while simultaneously enhancing cellular regenerative function and viability, this study is a proponent of clinical translation for ASC-based therapies.

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In Vivo Biocompatibility of Electrospun Biodegradable Dual Carrier (Antibiotic + Growth Factor) in a Mouse Model—Implications for Rapid Wound Healing

Pharmaceutics ◽

10.3390/pharmaceutics11040180 ◽

2019 ◽

Vol 11 (4) ◽

pp. 180 ◽

Cited By ~ 14

Author(s):

Charu Dwivedi ◽

Himanshu Pandey ◽

Avinash C. Pandey ◽

Sandip Patil ◽

Pramod W. Ramteke ◽

...

Keyword(s):

Wound Healing ◽

Growth Factor ◽

Drug Loading ◽

Physicochemical Characterization ◽

Diabetic Wound ◽

Scanning Calorimetry ◽

Rapid Healing ◽

Diabetic Wound Healing

Tissue engineering technologies involving growth factors have produced one of the most advanced generations of diabetic wound healing solutions. Using this approach, a nanocomposite carrier was designed using Poly(d,l-lactide-co-glycolide) (PLGA)/Gelatin polymer solutions for the simultaneous release of recombinant human epidermal growth factor (rhEGF) and gentamicin sulfate at the wound site to hasten the process of diabetic wound healing and inactivation of bacterial growth. The physicochemical characterization of the fabricated scaffolds was carried out using scanning electron microscopy (SEM) and X-ay diffraction (XRD). The scaffolds were analyzed for thermal stability using thermogravimetric analysis and differential scanning calorimetry. The porosity, biodegradability, and swelling behavior of the scaffolds was also evaluated. Encapsulation efficiency, drug loading capacity, and in vitro drug release were also investigated. Further, the bacterial inhibition percentage and detailed in vivo biocompatibility for wound healing efficiency was performed on diabetic C57BL6 mice with dorsal wounds. The scaffolds exhibited excellent wound healing and continuous proliferation of cells for 12 days. These results support the applicability of such systems in rapid healing of diabetic wounds and ulcers.

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