scholarly journals Bioprospecting Studies of Halopholic Bacteria – Streptomyces sp. MA05 and Halobacterium sp. MA06

2020 ◽  
Vol 2 (1) ◽  
pp. 83
Keyword(s):  
Gene Sequencing ◽  
Halobacterium Salinarum ◽  
Enzyme Analysis ◽  
Streptomyces Sp ◽  
Growth State ◽  
Bioactive Natural Products ◽  
Salt Pan ◽  
Hypersaline Condition ◽  
Halophilic Actinomycetes ◽  
16Srrna Gene

Halophile organisms such as Halobacterium salinarum and halophilic Actinomycetes flourish under the hostile hypersaline condition and are well known for their ability to produce novel bioactive compounds for bioprospecting analysis such as antibacterial, antifungal, anticancer, and enzyme analysis. In this present study, the objective is to isolate two types of halophiles, namely Halobacterium sp. MA06 and Streptomyces sp. MA05 based on their 16SrRNA gene sequencing. The two potential halophiles were isolated from the salt pan of Chennai, India. In order to evaluate enzyme analysis and pigment production of both the organism, optimization of the growth state of both species was performed on complex medium with various additives and different concentrations of sodium chloride and magnesium sulfate. The result showed that Halobacterium sp. MA06 produced orange colored pigment after characterization using GC-MS analysis. For Streptomyces sp. MA06, it was found to produce the enzyme amylase, hence Streptomyces sp. MA06 was subjected to producing the enzyme amylase, and the produced amylase will be characterized. The antimicrobial and anticancer activity was then carried out on the extract of Streptomyces sp. MA05 and the pigment of Halobacterium sp. MA06. In conclusion, the pigment from Halobacterium sp. MA06 and the enzyme amylase from Streptomyces sp. MA05 could be a novel bio-sourced for bioprospecting of bioactive natural products.

scholarly journals Selective Translation of Low Abundance and Upregulated Transcripts in Halobacterium salinarum

mSystems ◽  
2020 ◽  
Vol 5 (4) ◽  
Author(s):  
Adrián López García de Lomana ◽  
Ulrike Kusebauch ◽  
Arjun V. Raman ◽  
Min Pan ◽  
Serdar Turkarslan ◽  
...  
Keyword(s):  
Ribosomal Proteins ◽  
Translational Regulation ◽  
Physiological State ◽  
Protein Composition ◽  
Ribosome Profiling ◽  
Halobacterium Salinarum ◽  
Active Growth ◽  
Content Type ◽  
Growth State ◽  
Selective Translation

ABSTRACT When organisms encounter an unfavorable environment, they transition to a physiologically distinct, quiescent state wherein abundant transcripts from the previous active growth state continue to persist, albeit their active transcription is downregulated. In order to generate proteins for the new quiescent physiological state, we hypothesized that the translation machinery must selectively translate upregulated transcripts in an intracellular milieu crowded with considerably higher abundance transcripts from the previous active growth state. Here, we have analyzed genome-wide changes in the transcriptome (RNA sequencing [RNA-seq]), changes in translational regulation and efficiency by ribosome profiling across all transcripts (ribosome profiling [Ribo-seq]), and protein level changes in assembled ribosomal proteins (sequential window acquisition of all theoretical mass spectra [SWATH-MS]) to investigate the interplay of transcriptional and translational regulation in Halobacterium salinarum as it transitions from active growth to quiescence. We have discovered that interplay of regulatory processes at different levels of information processing generates condition-specific ribosomal complexes to translate preferentially pools of low abundance and upregulated transcripts. Through analysis of the gene regulatory network architecture of H. salinarum, Escherichia coli, and Saccharomyces cerevisiae, we demonstrate that this conditional, modular organization of regulatory programs governing translational systems is a generalized feature across all domains of life. IMPORTANCE Our findings demonstrate conclusively that low abundance and upregulated transcripts are preferentially translated, potentially by environment-specific translation systems with distinct ribosomal protein composition. We show that a complex interplay of transcriptional and posttranscriptional regulation underlies the conditional and modular regulatory programs that generate ribosomes of distinct protein composition. The modular regulation of ribosomal proteins with other transcription, translation, and metabolic genes is generalizable to bacterial and eukaryotic microbes. These findings are relevant to how microorganisms adapt to unfavorable environments when they transition from active growth to quiescence by generating proteins from upregulated transcripts that are in considerably lower abundance relative to transcripts associated with the previous physiological state. Selective translation of transcripts by distinct ribosomes could form the basis for adaptive evolution to new environments through a modular regulation of the translational systems.

scholarly journals New Antibacterial Bagremycins F and G from the Marine-Derived Streptomyces sp. ZZ745

Marine Drugs ◽  
2018 ◽  
Vol 16 (9) ◽  
pp. 330 ◽  
Author(s):  
Di Zhang ◽  
Chenyan Shu ◽  
Xiaoyuan Lian ◽  
Zhizhen Zhang
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Natural Products ◽  
Spectroscopic Analysis ◽  
Optical Rotation ◽  
Marine Microorganisms ◽  
Streptomyces Sp ◽  
Bioactive Natural Products ◽  
Marine Actinomycete

As part of our research to discover novel bioactive natural products from marine microorganisms, five bagremycin analogues, including the previously unreported bagremycins F (1) and G (2), were isolated from a marine actinomycete Streptomyces sp. ZZ745. The structures of these compounds were determined by means of NMR spectroscopic analysis, HRESIMS data, and optical rotation. Both bagremycins F (1) and G (2) showed antibacterial activity against Escherichia coli, with MIC values of 41.8 and 61.7 μM, respectively.

scholarly journals Tay-Sachs carrier screening in the genomics age: Gene sequencing versus enzyme analysis in non-Jewish individuals

2013 ◽  
Vol 03 (01) ◽  
pp. 61-66 ◽  
Author(s):  
Charles M. Strom ◽  
Noh Jin Park ◽  
Craig Morgan ◽  
Raynah Lobo ◽  
Beryl Crossley ◽  
...  
Keyword(s):  
Gene Sequencing ◽  
Carrier Screening ◽  
Enzyme Analysis

scholarly journals Antimicrobial and Enzymatic potential of Streptomyces sp. KAS-1 isolated from the microbiologically unexplored estuary of Kali river ecosystem

2020 ◽  
Vol 11 (2) ◽  
pp. 1655-1666
Author(s):  
Sreenivasa Nayaka ◽  
Shashiraj Kareyallappa Nagaraja ◽  
Bidhayak Chakraborty ◽  
Meghashyama Prabhakara Bhat ◽  
Pallavi Sathyanarayana Swamy ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Carbon Sources ◽  
Hydrolytic Enzymes ◽  
River Estuary ◽  
Gene Sequencing ◽  
Rrna Gene ◽  
Pathogenic Microorganisms ◽  
Streptomyces Sp ◽  
Moderately Thermophilic ◽  
Kali River

In the present study, a Streptomyces sp. was isolated from sediment soil samples of the Kali river estuary, Karwar, Karnataka, India. The isolated Actinomycetes strains were screened against pathogenic microorganisms and for the production of hydrolytic enzymes. Later, the strongest strain Streptomyces sp.KAS-1 was taken for further analysis based on primary characterizations. The morphological, physiological and biochemical characterizations of Streptomyces sp.KAS-1 were carried out, followed by molecular characterization through 16S rRNA gene sequencing. The 16S ribosomal RNA gene sequencing and analysis of the phylogenetic tree showed a 98.97%sequence similarity with Streptomyces thermocarboxydus strain SDT64(KJ018992). Finally, the intracellular methanol extract was characterized through UV-Visible and FTIR spectrophotometer. The Streptomyces sp.is a gram-positive, aerobic, non-motile, spore-forming bacterium and it was designated as Streptomyces sp.KAS-1 and was found to possess a strong antimicrobial activity against pathogenic microorganisms. The characterizations revealed the Streptomyces sp.KAS-1was moderately thermophilic, neutrophile organism having the capacity to degrade a broad range of carbon sources. The UV-Visiblespectrum ofmethanol extract of Streptomyces sp.KAS-1revealed the presence of Muconomycin-Band the presence of different functional groups such as aldehydes, amines, and alkyl halides were indicated by the FTIR spectrum.

scholarly journals Discovery of Novel Glycerolated Quinazolinones from Streptomyces sp. MBT27

2018 ◽  
Author(s):  
Nataliia Machushynets ◽  
Changsheng Wu ◽  
Somayah S. Elsayed ◽  
Thomas Hankemeier ◽  
Gilles P. van Wezel
Keyword(s):  
Natural Products ◽  
Natural Product ◽  
Secondary Metabolite ◽  
Anthranilic Acid ◽  
Growth Conditions ◽  
Secondary Metabolite Production ◽  
Streptomyces Sp ◽  
Metabolite Production ◽  
Bioactive Natural Products ◽  
Interesting Family

AbstractActinobacteria are a major source of novel bioactive natural products. A challenge in the screening of these microorganisms lies in finding the favorable growth conditions for secondary metabolite production and dereplication of known molecules. Here, we report that Streptomyces sp. MBT27 produces 4-quinazolinone alkaloids in response to elevated levels of glycerol, whereby quinazolinones A (1) and B (2) form a new sub-class of this interesting family of natural products. Global Natural Product Social molecular networking (GNPS) resulted in a quinazolinone-related network that included anthranilic acid (3), anthranilamide (4), 4(3H)-quinazolinone (5) and 2,2-dimethyl-1,2-dihydroquinazolin-4(3H)-one (6). Actinomycins D (7) and X2 (8) were also identified in the extracts of Streptomyces sp. MBT27. The induction of quinazolinone production by glycerol combined with biosynthetic insights provide evidence that glycerol is integrated into the chemical scaffold. The unprecedented 1,4-dioxepane ring that is spiro-fused into the quinazolinone backbone, is most likely formed by intermolecular etherification of two units of glycerol. Our work underlines the importance of varying the growth conditions for the discovery of novel natural products and for understanding their biosynthesis.

scholarly journals High throughput 16SrRNA gene sequencing reveals the correlation between Propionibacterium acnes and sarcoidosis

2017 ◽  
Vol 18 (1) ◽  
Author(s):  
Meng-Meng Zhao ◽  
Shan-Shan Du ◽  
Qiu-Hong Li ◽  
Tao Chen ◽  
Hui Qiu ◽  
...  
Keyword(s):  
High Throughput ◽  
Propionibacterium Acnes ◽  
Gene Sequencing ◽  
16Srrna Gene

scholarly journals Isolation, Identification and Characterization of Streptomyces sp. SN-2

2017 ◽  
Vol 14 (4) ◽  
pp. 1401-1407
Author(s):  
Bantanahal Talari Vishwanatha ◽  
Girish Babu K ◽  
Padmashri Swathi B Malagi ◽  
Chethan J Dandin ◽  
Sreenivasa Nayaka
Keyword(s):  
Ribosomal Rna ◽  
Gene Sequence ◽  
Gene Sequencing ◽  
University Campus ◽  
Streptomyces Sp ◽  
Restriction Sites ◽  
16S Ribosomal Rna Gene ◽  
Identification And Characterization ◽  
Positive Results

ABSTRACT: The present investigation is carried out for the isolation and molecular characterization of actinomycetes from the soil samples from Karnatak University campus, Dharwad, Karnataka, India. Totally four actinomycetes isolated, out of four isolated only one actinomycetes strain (SN-2) showed positive results for antimicrobial activity. SN-2 isolated actinomycetes was further analysed for morphological, physiological, biochemical and 16S ribosomal RNA gene sequencing. The SN-2 strain gene sequence was predicted with secondary structure and restriction sites were identified with the help of Genebee and NEBcutter tools. This investigation clearly indicates that the isolated strain SN-2 as Streptomyces sp.

scholarly journals Correlation of Streptococcus Mutans by 16SrRNA Gene Sequencing in Children with Different Caries Experience- A PCR Based Study

2021 ◽  
pp. 27-36
Author(s):  
Dhanalakshmi Ravikumar ◽  
Pratibha Ramani ◽  
R. Gayathri ◽  
Selvaraj Jayaraman
Keyword(s):  
Early Childhood ◽  
Streptococcus Mutans ◽  
Negative Correlation ◽  
Early Childhood Caries ◽  
Gene Sequencing ◽  
Fold Change ◽  
Group I ◽  
16Srrna Gene ◽  
Severe Early Childhood Caries ◽  
Childhood Caries

Background and Objectives: Early Childhood Caries is considered as a common chronic disease in children. Since some research had concluded that children with higher levels of Streptococcus mutans are associated with a higher incidence of decayed, missing, and filled teeth, the present study was undertaken to analyze the salivary Streptococcus mutans of children with different caries status using species specific 16S rRNA gene sequencing technology to evaluate the quantity of Streptococcus mutans with respect to different caries status. Materials and Methods: Children between 3-6 years were selected and divided into 3 groups, Group I- Caries free, Group II-Early Childhood caries and Group III- Severe Early childhood caries. The caries status was assessed using dmfs and the severity of caries was assessed using pufa index. Salivary samples were collected to isolate DNA and Real time PCR was done with 16SrRNA primer specific to Streptococcus mutans to estimate the quantity of Streptococcus mutans in children with different caries status. Results: There was a significant difference in the mean CT values among the study groups (p < 0.001). Post hoc Tukey test revealed that, Group I had a significantly higher mean CT values than that of Groups II and III. Pearson's correlation analysis was carried out to correlate dmfs value with CT values and to correlate the pufa value with the fold change. There was a negative correlation between dmfs score and CT values and positive correlation between pufa score and fold change. Conclusion: There was a higher expression of 16SrRNA gene in Severe Early childhood caries group, followed by Early childhood caries and caries free group, indicating a high level of Streptococcus mutans in Severe Early childhood caries group and there was a negative correlation between dmfs score and CT values and a positive correlation between pufa score and fold change.

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