scholarly journals Status of Extended Spectrum Beta-Lactamase (ESBL) Producing Bacteria Isolated from Surgical and Burn Wound at Tertiary Care Hospital in Dhaka City

2018 ◽  
Vol 5 (1) ◽  
pp. 21-26
Author(s):  
Mostaqimur Rahman ◽  
Hafiza Sultana ◽  
Md Abdullahil Mosawuir ◽  
Latifa Akhter ◽  
Md Abdullah Yusuf
Keyword(s):  
Tertiary Care ◽  
Care Hospital ◽  
Beta Lactamase ◽  
Surgical Wound ◽  
Burn Wound ◽  
Klebsiella Species ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
Proteus Species

Background: Surgical and burn wound infection are the most common infection in the hospital settings. Objective: The aim of the present study was to see the status of extended spectrum beta-lactamase (ESBL) producing bacteria isolated from patients presented with surgical and burn wound infection.Methodology: This cross-sectional study was conducted in the Department of Microbiology, BSMMU from January to December 2006, at a period of one year. This study was carried out to detect extended spectrum β-lactamase producing Gram negative bacteria rapidly by using a kit containing chromogenic cephalosporin directly from primary culture by comparison with phenotypic confirmatory method.Result: Total 181 samples were collected from patients with wound infections of which 170(93.9%) bacteria were isolated. Among individual samples ESBLs positive strains were highest in surgical wound which was 22(31.42%) and 24(28.24%) isolates respectively. From surgical wound swab ESBL was found 3(42.9%) isolates from Klebsiella species. ESBL producing E. coli was found in 12(35.3%) isolates. Pseudomonas species showed in 2(22.2%) isolates and 1(33.3%) isolate of Acinetobactor species. ESBL positive E. coli was found in 5(45.45%) isolates from burn wound. ESBL positive Proteus species was detected in 11(28.94%) isolates from burn wound.Conclusion: Most common bacteria isolated from the infected surgical and burn wound are E. coli and Proteus species, though Klebsiella species is the most common ESBL producing bacteria isolated from both infected surgical and burn wound.Bangladesh Journal of Infectious Diseases 2018;5(1):21-26

PHENOTYPIC DETECTION OF EXTENDED SPECTRUM BETA LACTAMASE AND THEIR ANTIBIOTIC SENSITIVITY PATTERN IN VARIOUS CLINICAL ISOLATES OF ENTEROBACTERIACEAE

2021 ◽  
pp. 54-58
Author(s):  
Deepali Gupta ◽  
Vipin Kumar Varshney ◽  
Shachi Shachi
Keyword(s):  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Research Centre ◽  
Care Hospital ◽  
Beta Lactamase ◽  
Klebsiella Species ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum ◽  
Sensitivity Pattern ◽  
Esbl Producers

Extended-spectrum β-lactamases (ESBLs) are a group of plasmid-mediated, diverse, complex and rapidly evolving enzymes that are posing a major therapeutic challenge today in the treatment of hospitalized and community-based patients. Enterobacteriaceae group is the main cause of bacterial infection and in this family Escherichia coli and Klebsiella species are the most common causes of nosocomial infections. ESBLs represents a major threat among multidrug-resistant (MDR) bacteria isolates. These ESBL producing pathogens are now recognized globally as major causes of nosocomial and community-acquired infections. ESBL detection is important from a therapeutic point of view and for infection control purposes. Carbapenems are often used to treat infections caused by ESBL producing E. coli and Klebsiella. This study was conducted in indoor patient department of Teerthanker Mahaveer Medical College & Research Centre, Moradabad, U.P., which is a tertiary care hospital. This study was conducted during the period from April 2015 to December 2017.A total no. of 350 gram negative Enterobacteriaceae were isolated in 800 no. of samples. They were screened for the beta-lactamase production. Among the 350 isolates 210 strains were ESBL producers. The major ESBL producers were E.coli (32%) followed by Klebsiella, Enterobacter, Citrobacter, Proteus. Multidrug resistance to Fluoroquinolones and Aminoglycosides were observed in the ESBL producing organism. The most common ESBL producing organism were from ICU.

scholarly journals Isolation and identification of various Extended Spectrum Beta-Lactamase producing uropathogens in a tertiary care hospital, Chhattisgarh

2019 ◽  
Vol 10 (4) ◽  
pp. 3244-3250
Author(s):  
Rajesh Kumar Sahu ◽  
Sagarika Pradhan ◽  
Shweta Sao ◽  
Ramanesh Murthy ◽  
Ekta Agrawal ◽  
...  
Keyword(s):  
Tertiary Care ◽  
Medical Science ◽  
Care Hospital ◽  
Human Beings ◽  
Isolation And Identification ◽  
Klebsiella Species ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
Esbl Producers

Extended Spectrum B-Lactamase producing bacteria has become a worldwide problem and made the treatment very much complicated due to their resistance ability against the wide number of antimicrobial drug. This study was undertaken to measure the emergence of ESBL producing bacteria in urine collected from the patient Chhattisgarh Institute of Medical Science, Bilaspur, C.G. A total of 141 Eterobactericeae groups isolates and identified. A Total of 81 ESBL producing uropathogens were identified. The most prevalence of ESBL producing bacteria was E. coli 59(41.84%) followed by Klebsiella species 12(8.51%), and Enterobacter species were 06(4.26%). ESBL producers are confirmed by the Phenotyping confirmatory disc diffusion test. Antimicrobial susceptibility testing by Kirby-Bauer method showed most resistance were ampicillin (88.9%), Ciprofloxacin (69.1%), amoxycillin clavulanic acid (76.5%) resistance to ESBL producers and least resistant to imipenem 15(18.5%). We are confident that this research will be helpful in human beings and further medical microbiological study.

scholarly journals Detection of Extended Spectrum Beta-lactamase (ESBL) Producing Gram Negative Bacteria from Clinical Specimens of Sir Salimullah Medical College and Mitford Hospital.

2017 ◽  
Vol 10 (1) ◽  
pp. 8-12
Author(s):  
Shikha Paul ◽  
Sanya Tahmina Jhora ◽  
Prashanta Prasun Dey ◽  
Bilkis Ara Begum
Keyword(s):  
Tertiary Care ◽  
Gram Negative Bacteria ◽  
Care Hospital ◽  
Beta Lactamase ◽  
Gram Negative ◽  
Clinical Specimens ◽  
Extended Spectrum Beta Lactamase ◽  
Medical College ◽  
Extended Spectrum ◽  
Esbl Producers

Detection of Extended spectrum beta lactamase (ESBL) enzyme producing bacteria in hospital settings is vital as ESBL genes are transmissible. This study was carried out to determine the distribution of ESBL producing gram negative isolates at a tertiary care hospital in Dhaka city which deals with the patients hailing from relatively low socioeconomic status.Onehundred and twenty four gram negative bacteria isolated from different clinical specimens from outpatient and inpatient departments of Sir Salimullah Medical College and Mitford Hospital (SSMC & MH) were tested for ESBL by E test ESBL method in the department of microbiology of Sir Salimullah medical college (SSMC) from March 2013 to August 2013.Out of 124 gram negative bacteria 69 (55.65%) were positive for ESBL. Among the ESBL producers, Esch.coli was the highest (46.38%) which was followed by Serratia spp (11.59%), Enterobacter spp (10.14%), Proteus spp, (8.70%), Acinetobacter spp.(7.24%) and Klebsiella spp.(5.79%). Out of 32 Esch.coli isolated from outpatient department, 10 (31.25%) were positive for ESBL. On the other hand out of 27 Esch. coli isolated from inpatient department, 22 (81.48%) were positive for ESBL. The difference was statistically significant (p<0.001).So the present study reveals that the distribution of ESBL producers is more among the hospitalized patients than the patients of the community.Bangladesh J Med Microbiol 2016; 10 (1): 8-12

scholarly journals Detection of CTX-M gene in extended spectrum beta lactamase (ESBL) producing Escherichia coli and Klebsiella species of different hospitals.

2012 ◽  
Vol 4 (2) ◽  
pp. 28-31 ◽  
Author(s):  
Nawshad Muhammad Wahidur Rahman ◽  
Afzalunnessa Binte Lutfor ◽  
Sanya Tahmina Jhora ◽  
Mahmuda Yasmin ◽  
Jalaluddin Ashraful Haq
Keyword(s):  
Escherichia Coli ◽  
Tertiary Care ◽  
Beta Lactamase ◽  
M Gene ◽  
Klebsiella Species ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum ◽  
Synergy Test ◽  
Tertiary Care Hospitals ◽  
Klebsiella Spp

A total of 200, non-duplicate ESBL producing strains (171 Escherichia coli and 29 Klebsiella spp.) from three tertiary care hospitals were detected using screening test & double disc synergy test. All isolates were screened for the detection of CTX-M type Extended spectrum Beta-lactamase (ESBL) using PCR. Among them 133 (66.5%) were positive for CTX-M type ESBLs which include 114 (66.66%) E.coli and 19 (65.51%) Klebsiella spp. This is the first report of identifying CTX-M gene in ESBL producing Escherichia coli and Klebsiella species of different hospitals.DOI: http://dx.doi.org/10.3329/bjmm.v4i2.10829 

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