scholarly journals Cytochrome c adsorption in a continuous flow system by using Cu(II)-chelated magnetic affinity particles

2021 ◽  
Vol 20 (1) ◽  
pp. 159-163
Author(s):  
Recep AKKAYA ◽  
Birnur AKKAYA

Background and methodology: In the current study magnetic poly(ethylene glycoldimethacrylate-N-methacryloyl-(L)-histidine methyl ester) poly(Egdma–Mah)) was used as ametal chelated affinity particles. Cu2+ ions loaded directly to MAH ligand of magnetic particlesfor the adsorption of cytochrome c (Cyt c) in a continuous flow system. Results: The maximumCyt c adsorption capacity on the magnetic particles and Cu2+-chelated magnetic particles were42 mg/g and 197 mg/g in phosphate buffer (pH 8.0), respectively. Cu2+ loading increased theCyt c adsorption capacity, significantly. Cyt c adsorption capacity decreased with increasedtemperature and with increasing magnetic field. According to reusability studies Cyt c moleculescould be reversibly adsorbed and desorbed five times. The binding isotherm was determined byscatchard analysis followed by application of Hill equation to the data obtained, then bindingconstant and nH Hill coefficient were calculated. Bangladesh Journal of Medical Science Vol.20(1) 2021 p.159-163

2021 ◽  
Vol 140 ◽  
pp. 107769
Author(s):  
Sujka Monika ◽  
Pankiewicz Urszula ◽  
Flisar Karel ◽  
Miklavčič Damijan ◽  
Haberl-Meglič Sasa

Author(s):  
Apisit Naramittanakul ◽  
Supacha Buttranon ◽  
A. Petchsuk ◽  
Pimchai Chaiyen ◽  
Nopphon Weeranoppanant

Immobilization methods have emerged as feasible solutions for increasing the re-usability of biocatalysts, and for simplifying their separation from the desired products. Immobilized biocatalysts can directly be applied to a...


2007 ◽  
Vol 90 (1) ◽  
pp. 85-91 ◽  
Author(s):  
Sung Hyun Kim ◽  
Sang Bum Kim ◽  
Gyung Soo Kim ◽  
Hyun Tae Jang ◽  
Sung Chang Hong

2018 ◽  
Vol 34 ◽  
pp. 02054 ◽  
Author(s):  
N. Idris ◽  
N.A. Lutpi ◽  
Y. S. Wong ◽  
T.N. Tengku Izhar

This research aims to study the acclimatization phase for biohydrogen production from palm oil mill effluent (POME) by adapting the microorganism to the new environment in continuous-flow system of thermophilic bioreactor. The thermophilic fermentation was continuously loaded with 0.4 L/day of raw POME for 35 days to acclimatize the microorganism until a steady state of biohydrogen production was obtained. The significance effect of acclimatization phase on parameter such as pH, microbial growth, chemical oxygen demand (COD), and alkalinity were also studied besides the production of biogas. This study had found that the thermophilic bioreactor reach its steady state with 1960 mL/d of biogas produced, which consist of 894 ppm of hydrogen composition.


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