scholarly journals A Bradford multiplexing method for protein estimation in fermented foods: Soy sauces

2015 ◽  
Vol 11 (1) ◽  
pp. 24 ◽  
Author(s):  
Shruti Shukla
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Microtiter Plate ◽  
Soy Sauce ◽  
Total Protein Content ◽  
Fermented Foods ◽  
Easy Method ◽  
Assay Procedure ◽  
Bioassay Procedure

<p>Proteins for foods, in addition to providing nutrition, should also possess specific functional properties that facilitate processing and serve as the basis of product performance. Soy protein is a major component of the diet of food and is increasingly important in the human diet. Hence, here in the present article, we are focusing a rapid and easy method for quantitative determination of total protein content with multiplex samples in any food products such as soy sauce or other traditional fermented foods. We described a bioassay procedure (Bradford method) for the evaluation of total protein content in foods. This method involves measurement of the protein efficiency ratio under standardized conditions. The experiment will provide researchers a scientific way to determine pretentious quality of variety of foods and/or health supplements.</p><p> </p><p><strong>VIDEO CLIPS</strong></p><p><a href="https://www.youtube.com/v/xVzg0rq4VxE">Requirement and sample preparation method:</a>   3 min 26 sec</p><p><a href="https://www.youtube.com/v/6plwYgGGFuE">Assay procedure:</a>                                         6 min   4 sec</p><p><a href="https://www.youtube.com/v/bV2tGWLh4ug">Measurement of absorbance using an ELISA microtiter plate reader:</a>5 min 1 sec</p>

Retardation Signal for Fluorescent Determination of Total Protein Content via Rapid and Sensitive Chip Moving Reaction Boundary Electrophoretic Titration

2014 ◽  
Vol 86 (6) ◽  
pp. 2888-2894 ◽  
Author(s):  
Houyu Wang ◽  
Yongting Shi ◽  
Jian Yan ◽  
Jingyu Dong ◽  
Si Li ◽  
...  
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Total Protein Content

scholarly journals Analysis of Fatty acid and Determination of Total Protein, Alkaloid, Saponin, Flavonoid of Bangladeshi Bombax ceiba Linn Leaves and Seeds

2016 ◽  
Vol 4 (04) ◽  
pp. 23-28
Author(s):  
Shahin Aziz ◽  
Shahal Ahmed ◽  
Sharmin Akter Lisa ◽  
Tanzima Parvin
Keyword(s):  
Fatty Acid ◽  
Protein Content ◽  
Total Protein ◽  
Petroleum Ether Extract ◽  
Folk Medicine ◽  
Fatty Acid Analysis ◽  
Total Protein Content ◽  
Saponin Content ◽  
Kjeldahl Method

Bombax ceiba Linn belongs to the family of Bombacaceae and is an important medicinal plant. In Bangladesh, Bombax ceiba Linn is locally known as “Shimul tree”. The whole part of the plant used as traditional folk medicine for the treatment of antidysentric, anti diahorreal and antipyretic effects. The present communication attempts to evaluate fatty acid analysis by GC-MS spectrophotometer, total protein content by Kjeldahl method and to quantify some active constituents i.e. alkaloid, saponin and flavonoid. The fatty acid compositions of the petroleum ether extract of leaves and seeds of Bombax ceiba grown in Bangaladesh were determined by gas chromatography- mass spectrophotometer. 8 compounds were identified from leaves and 13 compounds were identified from the seeds. For both cases Palmitic acid showed higher value. The findings from present study showed the protein content for seeds have higher value (18.89%) than leaves of Bombax ceiba . The present investigation showed that both leaves and seeds of Bombax ceiba contain phytochemicals such as flavanoids, alakaloids and saponins in appreciable quantities. The flavonoid content of leaves was 5.97% and for the case of seeds (5.72%), the alkaloid content for leaves was (9.73%) and for seeds (31.44), the saponin content for the case of leaves (13.90%) and for the case of seeds was(43.58%).

Determination of total protein content in soya-bean milk via visual moving reaction boundary titration

2013 ◽  
Vol 31 (11) ◽  
pp. 1064 ◽  
Author(s):  
Chengye GUO ◽  
Houyu WANG ◽  
Lei ZHANG ◽  
Liuyin FAN ◽  
Chengxi CAO
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Soya Bean ◽  
Total Protein Content

scholarly journals Colorimetric determination of total protein content in serum based on the polydopamine/protein adsorption competition on microplates

Talanta ◽  
2019 ◽  
Vol 198 ◽  
pp. 15-22 ◽  
Author(s):  
Pasquale Palladino ◽  
Alvaro Brittoli ◽  
Emanuela Pascale ◽  
Maria Minunni ◽  
Simona Scarano
Keyword(s):  
Protein Content ◽  
Protein Adsorption ◽  
Total Protein ◽  
Total Protein Content ◽  
Colorimetric Determination

Seromucoid Determination: Variables and Modifications

1960 ◽  
Vol 6 (3) ◽  
pp. 189-198 ◽  
Author(s):  
T M Asher ◽  
G R Cooper
Keyword(s):  
Environmental Factors ◽  
Protein Content ◽  
Perchloric Acid ◽  
Total Protein ◽  
Standard Procedure ◽  
Total Protein Content ◽  
Inflammatory Condition ◽  
Freezing And Thawing ◽  
Significant Difference

Abstract The technic of Winzler (1) for the determination of seromucoid has been studied to measure its reproducibility and the effect of certain environmental factors. Values obtained are fairly reproducible (a difference of 45 mg./l00 ml. BSAP between two specimens indicates a significant difference at the P = 0.05 level). Variables such as temperature, freezing and thawing, speed of addition of perchloric acid, and total protein content of serum have no practical influence on the final SM values. A modification in technic involving determinations on three different dilutions of a specimen gives differences greater than those found with the standard procedure for sera from patients with an inflammatory condition.

scholarly journals Determination of total protein content in biomedical products by the PDMS-assisted lab-in-a-syringe assay using 3D printed scaffolds removal

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Ester Drastíková ◽  
Klára Konderlová ◽  
Andrea Šebestová ◽  
Daniel Baron ◽  
Petra Švecová ◽  
...  
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Low Cost ◽  
Total Protein Content ◽  
Fabrication Procedure ◽  
One Step ◽  
3D Printed ◽  
Bradford Assay ◽  
The One

AbstractThe aim of our work was to develop a low-cost, portable device for the fast and easy determination of total protein content by using PDMS-based lab-in-a-syringe technology with removal of 3D-printed channels. We proposed two designs with a one-step PDMS curing and a two-step PDMS-curing fabrication procedure. The one-step PDMS microdevices were found to be the best in the view of preparation, repeatability, and stability of the reagent. This design was then applied for the determination of total protein content in biomedical products using the Bradford assay.

Determination of fat and total protein content in milk using conventional digital imaging

Talanta ◽  
2014 ◽  
Vol 121 ◽  
pp. 144-152 ◽  
Author(s):  
Sergey Kucheryavskiy ◽  
Anastasiia Melenteva ◽  
Andrey Bogomolov
Keyword(s):  
Protein Content ◽  
Digital Imaging ◽  
Total Protein ◽  
Total Protein Content
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